FastQCFastQC Report
Thu 26 May 2016
SRR936404_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936404_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2857144
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96380.33732986506805396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76310.2670848931660427No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44690.15641493743402504No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32180.11262995494801802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA161150.030.4609971
GGTATCA110350.027.5170971
ATCAACG189200.025.5440143
TATCAAC190500.025.5180322
TCAACGC190750.025.4129684
CAACGCA193800.024.9652865
AACGCAG198300.024.4706556
GTACATG132250.024.1102091
TACATGG134700.023.389042
GAGTACT95250.023.05157312-13
ACGCAGA219200.022.0940937
CGCAGAG222200.021.8206238
ACATGGG136850.021.8074153
GTACTTT102150.021.3294114-15
GCAGAGT231450.020.3947479
AGTACTT101550.020.00689712-13
GTGTAGC17350.019.720011
AGAGTAC184000.018.70273610-11
GTACCGT1801.2287237E-418.4828156
ACTTTTT124100.017.84274316-17
Produced by FastQC (version 0.11.5)