##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936404_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2857144 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.24304515278194 31.0 31.0 34.0 28.0 34.0 2 31.31001867599253 31.0 31.0 34.0 28.0 34.0 3 31.25111159955536 31.0 31.0 34.0 27.0 34.0 4 34.9135787345685 37.0 35.0 37.0 32.0 37.0 5 34.94937112025155 37.0 35.0 37.0 32.0 37.0 6 35.00715819713672 37.0 35.0 37.0 32.0 37.0 7 34.86913505234598 37.0 35.0 37.0 32.0 37.0 8 34.85022805990877 37.0 35.0 37.0 32.0 37.0 9 36.31982987206805 39.0 37.0 39.0 32.0 39.0 10-11 36.19364582254167 39.0 35.0 39.0 32.0 39.0 12-13 36.081058042576785 39.0 35.0 39.0 30.5 39.0 14-15 37.06546432381427 39.0 36.0 41.0 31.0 41.0 16-17 36.9747592350963 39.0 36.0 41.0 31.0 41.0 18-19 36.84585936165625 39.0 36.0 41.0 30.0 41.0 20-21 36.82096247161501 39.0 36.0 41.0 30.0 41.0 22-23 36.812483025006784 39.0 36.0 41.0 30.0 41.0 24-25 36.92686210425516 39.0 36.0 41.0 30.5 41.0 26-27 36.85561753275299 39.0 36.0 41.0 30.0 41.0 28-29 36.73935055425978 39.0 36.0 41.0 30.0 41.0 30-31 36.54687215625114 39.0 36.0 41.0 30.0 41.0 32-33 36.38472194611123 39.0 35.0 41.0 30.0 41.0 34-35 36.19183229826708 39.0 35.0 40.0 30.0 41.0 36-37 35.99240097803961 38.5 35.0 40.0 29.0 41.0 38-39 35.79954090518363 38.0 35.0 40.0 28.0 41.0 40-41 35.56862534754986 38.0 35.0 40.0 27.0 41.0 42-43 35.295521856791254 38.0 34.0 40.0 26.5 41.0 44-45 35.14845174061931 38.0 34.0 40.0 26.0 41.0 46-47 35.00540802283679 38.0 34.0 40.0 25.0 41.0 48-49 34.76805719277712 38.0 34.0 40.0 24.0 41.0 50-51 33.79725278109889 37.5 33.0 39.5 23.5 40.5 52-53 33.90574853770059 37.5 33.0 39.5 23.0 40.5 54-55 34.342549062980375 38.0 34.0 40.0 23.0 41.0 56-57 34.19928782028487 38.0 33.5 40.0 23.0 41.0 58-59 33.97403911038435 38.0 33.0 40.0 22.5 41.0 60-61 33.68827034969186 37.0 33.0 40.0 22.0 41.0 62-63 33.35347920860832 37.0 32.5 40.0 21.5 41.0 64-65 32.99159002836399 36.0 32.0 39.0 20.0 41.0 66-67 32.63010544795782 36.0 32.0 39.0 19.0 41.0 68-69 32.19985114505954 35.0 31.0 39.0 18.0 40.0 70-71 31.806984352206257 35.0 31.0 38.0 15.5 40.0 72-73 31.391437043425185 35.0 31.0 37.5 12.5 39.5 74-75 30.977350634059746 35.0 30.5 37.0 9.5 39.0 76-77 30.518555767577695 34.5 30.0 36.0 7.5 39.0 78-79 30.05817417673033 34.0 29.5 36.0 6.5 38.5 80-81 29.618350877659648 34.0 29.0 35.5 2.0 37.0 82-83 29.221873136250743 34.0 29.0 35.0 2.0 37.0 84-85 28.853306483677407 34.0 29.0 35.0 2.0 36.0 86-87 28.4927672528931 34.0 29.0 35.0 2.0 36.0 88-89 28.145001966999214 34.0 27.5 35.0 2.0 36.0 90-91 27.789102159359135 34.0 27.0 35.0 2.0 35.0 92-93 27.37361067555573 33.0 26.0 35.0 2.0 35.0 94-95 26.87850437359825 33.0 25.0 35.0 2.0 35.0 96-97 26.280424962830015 33.0 24.5 35.0 2.0 35.0 98-99 25.528565588573763 33.0 22.0 35.0 2.0 35.0 100-101 22.928507103597155 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5961802366940354 1101 2 0.529077127644566 1101 3 0.604632358093582 1101 4 0.6107254913231301 1101 5 0.538839073404354 1101 6 0.5124166010636628 1101 7 0.6351735467577626 1101 8 0.5754141431442648 1101 9 0.8175352511237293 1101 10-11 0.8006482770727956 1101 12-13 0.8811431928831297 1101 14-15 1.0704064882558484 1101 16-17 1.0534393566047058 1101 18-19 1.2424248715111617 1101 20-21 1.132408897873887 1101 22-23 1.0050097970611844 1101 24-25 0.8796231570229835 1101 26-27 0.8342385659728961 1101 28-29 0.8621865779013334 1101 30-31 0.7745403405473255 1101 32-33 0.7134777529539917 1101 34-35 0.7717257649428362 1101 36-37 0.6891276367691361 1101 38-39 0.8249290433491296 1101 40-41 0.8685023378977377 1101 42-43 0.9395978793335331 1101 44-45 0.7865345802146564 1101 46-47 0.8077259767895058 1101 48-49 0.820584896917687 1101 50-51 0.7358011191022271 1101 52-53 0.9055443476270142 1101 54-55 0.9465490699859558 1101 56-57 1.0880523153423027 1101 58-59 0.807905252174919 1101 60-61 0.6944045599468112 1101 62-63 0.8757269863439845 1101 64-65 0.6499652701525349 1101 66-67 0.7776478634385313 1101 68-69 0.826962149165233 1101 70-71 0.7328386855069127 1101 72-73 0.7967482953934848 1101 74-75 0.7065147932536817 1101 76-77 0.7817737387183215 1101 78-79 0.7560916791600611 1101 80-81 0.8257919716719258 1101 82-83 0.8299585944197894 1101 84-85 0.8483917184883971 1101 86-87 0.9332999035522249 1101 88-89 0.9329329902763064 1101 90-91 0.7609422831466084 1101 92-93 0.7649442694040651 1101 94-95 1.0222691063906417 1101 96-97 0.9317724517555064 1101 98-99 1.031493331459039 1101 100-101 1.2692071624604466 1102 1 0.42502794647836595 1102 2 0.43415173769459514 1102 3 0.4673944901311984 1102 4 0.39541467732552604 1102 5 0.47301245899794964 1102 6 0.42032693977047586 1102 7 0.5462252449862177 1102 8 0.37914732419603325 1102 9 0.5942820110444416 1102 10-11 0.645124857983518 1102 12-13 0.5328280562025043 1102 14-15 0.7061096297810536 1102 16-17 0.3993910731135841 1102 18-19 0.5895802235582579 1102 20-21 0.6206879486840506 1102 22-23 0.44672531735438525 1102 24-25 0.6989668183305255 1102 26-27 0.5076001354597324 1102 28-29 0.3246167353945637 1102 30-31 0.217954227366711 1102 32-33 0.4006292721890219 1102 34-35 0.2020985362924037 1102 36-37 0.41457933146300974 1102 38-39 0.16731729395760198 1102 40-41 0.5322605592520233 1102 42-43 0.3920298648682561 1102 44-45 0.5273893746262601 1102 46-47 0.5029041445617324 1102 48-49 0.6542565375876919 1102 50-51 0.6020054919321112 1102 52-53 0.4180533428614055 1102 54-55 0.6858652167837604 1102 56-57 0.5624110234747661 1102 58-59 0.44413118043064514 1102 60-61 0.5027197739464029 1102 62-63 0.4840713606429361 1102 64-65 0.6055944505548183 1102 66-67 0.4028602559030219 1102 68-69 0.3954920006409992 1102 70-71 0.18964791915988144 1102 72-73 0.4576785752163772 1102 74-75 0.32896087845588085 1102 76-77 0.4873250754805696 1102 78-79 0.3446187029704397 1102 80-81 0.4762507769501596 1102 82-83 0.30551138367422226 1102 84-85 0.49142514072014265 1102 86-87 0.2394528371722231 1102 88-89 0.3177386517926095 1102 90-91 0.5078334252086272 1102 92-93 0.3977572276225523 1102 94-95 0.5038058873034572 1102 96-97 0.5446920659780012 1102 98-99 0.7634673787926083 1102 100-101 1.2010864940489476 1103 1 0.3663981774590539 1103 2 0.4360095033191662 1103 3 0.43411542751696075 1103 4 0.44907000618943727 1103 5 0.5223252714504767 1103 6 0.4874878940547802 1103 7 0.5390610806237746 1103 8 0.18900913516322504 1103 9 0.4360070147081174 1103 10-11 0.34570073956061975 1103 12-13 0.20292344061473244 1103 14-15 0.6719484398405129 1103 16-17 0.8396567258679468 1103 18-19 0.8360598468088938 1103 20-21 0.7877696709392836 1103 22-23 0.5983699992801874 1103 24-25 0.47493664574319894 1103 26-27 0.6535113386861298 1103 28-29 0.5679532734380146 1103 30-31 0.6188297520239203 1103 32-33 0.6324153800659005 1103 34-35 0.6549976716648302 1103 36-37 0.6257027353482769 1103 38-39 0.4199597304391176 1103 40-41 0.6215008926492303 1103 42-43 0.6779571077493145 1103 44-45 0.5526668075085226 1103 46-47 0.8451313805180476 1103 48-49 0.8659932918551334 1103 50-51 0.7644373784220306 1103 52-53 0.7333821101538192 1103 54-55 0.8295346796539533 1103 56-57 0.8763532302585517 1103 58-59 0.6919274188417432 1103 60-61 0.7358259325188996 1103 62-63 0.9249284622429883 1103 64-65 0.8635951945629046 1103 66-67 0.7368470924354256 1103 68-69 0.9868863072077545 1103 70-71 0.9322633169062726 1103 72-73 0.9271867515215959 1103 74-75 0.8238647988845891 1103 76-77 0.7126203838815783 1103 78-79 0.7177456787386447 1103 80-81 0.8810232720558631 1103 82-83 0.7763081540564727 1103 84-85 0.877918710096214 1103 86-87 0.7643146239833136 1103 88-89 0.7060432849875014 1103 90-91 0.8521901024664054 1103 92-93 0.7823923872112069 1103 94-95 0.8793944239963132 1103 96-97 0.9753668855989837 1103 98-99 1.3783210759920586 1103 100-101 1.620899171495683 1104 1 0.37657837426921503 1104 2 0.40735448693100906 1104 3 0.5663631522999033 1104 4 0.6037534718565567 1104 5 0.45273368668231484 1104 6 0.3915115938227558 1104 7 0.4817962973955403 1104 8 0.46719077572999623 1104 9 0.61383520132825 1104 10-11 0.6694469834780961 1104 12-13 0.6925511975333194 1104 14-15 0.7407413383679753 1104 16-17 0.6852180623704314 1104 18-19 0.80351178343669 1104 20-21 0.884772279554582 1104 22-23 0.6656357255597598 1104 24-25 0.8186867845049548 1104 26-27 0.476033254585829 1104 28-29 0.327871805221271 1104 30-31 0.3214058468568197 1104 32-33 0.45774958709611724 1104 34-35 0.5128214242521452 1104 36-37 0.5206608784590614 1104 38-39 0.3944654689910365 1104 40-41 0.5372496172568901 1104 42-43 0.5787306635751079 1104 44-45 0.5766086817152001 1104 46-47 0.6095230627207471 1104 48-49 0.5448408091244943 1104 50-51 0.36375934354646944 1104 52-53 0.30491984838264585 1104 54-55 0.4804439312623714 1104 56-57 0.5801321912908648 1104 58-59 0.45555303042428363 1104 60-61 0.5053005239461399 1104 62-63 0.5787057739927093 1104 64-65 0.5522085792523512 1104 66-67 0.5521164995754262 1104 68-69 0.5517931893468457 1104 70-71 0.43786405857597543 1104 72-73 0.5828195061893311 1104 74-75 0.5413795351188462 1104 76-77 0.4430668700532969 1104 78-79 0.442248721347859 1104 80-81 0.5942184737430374 1104 82-83 0.4758614099200429 1104 84-85 0.5795792416430778 1104 86-87 0.5111074036778547 1104 88-89 0.41558120252295083 1104 90-91 0.6753625334814579 1104 92-93 0.544533855905815 1104 94-95 0.64889423041706 1104 96-97 0.6283068692626337 1104 98-99 0.5536135963474855 1104 100-101 1.1633201470717083 1105 1 0.35969560583712834 1105 2 0.29288690011592067 1105 3 0.3950119234940992 1105 4 0.5106936871679579 1105 5 0.5054972517998735 1105 6 0.43628604930454884 1105 7 0.4084152927199227 1105 8 0.4295206397876399 1105 9 0.3234919759834014 1105 10-11 0.47122051548362265 1105 12-13 0.4822052475175127 1105 14-15 0.7366058702237481 1105 16-17 0.5609097336128812 1105 18-19 0.5246330791321228 1105 20-21 0.4673817031722862 1105 22-23 0.3907137218658505 1105 24-25 0.3474054403894158 1105 26-27 0.4273929901887499 1105 28-29 0.45290185979789044 1105 30-31 0.29094059369121084 1105 32-33 0.3552378980497082 1105 34-35 0.245886554287587 1105 36-37 0.3932153302802277 1105 38-39 0.07874328559363875 1105 40-41 0.249871603479626 1105 42-43 0.06840062480574005 1105 44-45 0.14652113866123528 1105 46-47 0.3702081406754516 1105 48-49 0.458439309099532 1105 50-51 0.35433198202631644 1105 52-53 0.5165661528136738 1105 54-55 0.4282703302041142 1105 56-57 0.3584756329454706 1105 58-59 0.4474057442797488 1105 60-61 0.3417444539616099 1105 62-63 0.5209226786001011 1105 64-65 0.5589632673503289 1105 66-67 0.44949935952318043 1105 68-69 0.5326627753632565 1105 70-71 0.5453263291628403 1105 72-73 0.4327752042874664 1105 74-75 0.40745457380501193 1105 76-77 0.5844655414122215 1105 78-79 0.594159318861962 1105 80-81 0.5493651899945569 1105 82-83 0.24203004417352503 1105 84-85 0.4473937019502401 1105 86-87 0.4770336254119911 1105 88-89 0.5683852217246006 1105 90-91 0.5697169528561581 1105 92-93 0.6715916064507503 1105 94-95 0.5638869923598513 1105 96-97 0.8742922433953098 1105 98-99 1.0733119274345206 1105 100-101 1.3812989195970395 1106 1 0.2227126624766349 1106 2 -0.011641083238593808 1106 3 0.1533742244584424 1106 4 0.23756817322611568 1106 5 0.3627562909466704 1106 6 0.2011162304612597 1106 7 0.3485145925130979 1106 8 0.4444327401589234 1106 9 0.4537039692055984 1106 10-11 0.46892584303589047 1106 12-13 0.46365926685417946 1106 14-15 0.3604026233572384 1106 16-17 0.278553356996305 1106 18-19 0.5149331277229834 1106 20-21 0.14467123620161715 1106 22-23 0.03731080392211794 1106 24-25 -0.1299602494762766 1106 26-27 -0.13343078071154935 1106 28-29 0.057991908637937684 1106 30-31 0.028677785637590603 1106 32-33 0.16893927603679515 1106 34-35 0.24528025865577519 1106 36-37 -0.06727282102425391 1106 38-39 0.20808313508655374 1106 40-41 0.014602889767033389 1106 42-43 4.213818264915403E-4 1106 44-45 -0.37138942299932154 1106 46-47 -0.03897584427383549 1106 48-49 -0.04372127124982228 1106 50-51 -0.049796372612242124 1106 52-53 -0.033739876191340556 1106 54-55 0.17659410110442053 1106 56-57 0.20203267114536771 1106 58-59 0.018268512463947673 1106 60-61 0.2186913590972921 1106 62-63 0.236805817569973 1106 64-65 0.13771037058722868 1106 66-67 0.07422681460778335 1106 68-69 0.14571051277324187 1106 70-71 -0.0061208064679689755 1106 72-73 0.025723610756283932 1106 74-75 0.129497539342875 1106 76-77 0.05119279092008355 1106 78-79 0.1254985578542609 1106 80-81 0.1075493244541974 1106 82-83 0.10177765200786482 1106 84-85 0.42491030382908335 1106 86-87 0.5309184027661544 1106 88-89 0.5807368015506711 1106 90-91 0.5125228572692073 1106 92-93 0.6074044755544357 1106 94-95 0.624286683371789 1106 96-97 0.676321223485516 1106 98-99 0.7496930293258295 1106 100-101 1.293990946957436 1107 1 0.15271571763685898 1107 2 0.19405502503996885 1107 3 0.26409230993230537 1107 4 0.1689753738856652 1107 5 0.23844627355221348 1107 6 0.28280445980399804 1107 7 0.3314284170363635 1107 8 0.2713559174641489 1107 9 0.4347524572083401 1107 10-11 0.5209334210614571 1107 12-13 0.345131332096436 1107 14-15 0.04821268757701347 1107 16-17 0.21801190277074767 1107 18-19 0.4788096194164808 1107 20-21 0.2418067548188958 1107 22-23 0.3125526451858107 1107 24-25 0.26002696183794427 1107 26-27 0.18598683512738035 1107 28-29 0.2950189153331948 1107 30-31 0.27166957048061846 1107 32-33 0.2879697002127841 1107 34-35 0.2725582337140011 1107 36-37 0.21491298491627475 1107 38-39 0.41569340103901453 1107 40-41 0.2847019291571016 1107 42-43 0.1756038086676881 1107 44-45 0.16425339652975168 1107 46-47 0.3705316915321859 1107 48-49 0.34149390261029566 1107 50-51 0.3865928885763168 1107 52-53 0.32556861362299827 1107 54-55 0.5347436258658007 1107 56-57 0.6315358468232191 1107 58-59 0.5778293984297136 1107 60-61 0.5855035680702514 1107 62-63 0.4959917939239631 1107 64-65 0.6154221323400009 1107 66-67 0.6311450637399787 1107 68-69 0.5638368244579226 1107 70-71 0.507548348849312 1107 72-73 0.48852772585752646 1107 74-75 0.33864229406263746 1107 76-77 0.3402947733966677 1107 78-79 0.47548451025824434 1107 80-81 0.5122054500504589 1107 82-83 0.437718311277667 1107 84-85 0.6511160558206974 1107 86-87 0.7583740069893423 1107 88-89 0.630989274464909 1107 90-91 0.5390943073342243 1107 92-93 0.6979792294912173 1107 94-95 0.6035880495964498 1107 96-97 0.7027009200267393 1107 98-99 1.0102119152823796 1107 100-101 1.4549538290635624 1108 1 0.028579013005874288 1108 2 0.05096852181010547 1108 3 0.1613446527194995 1108 4 0.2286071654871975 1108 5 0.2217628579214974 1108 6 0.1922650397730692 1108 7 0.25028165062030894 1108 8 0.33516233665969963 1108 9 -0.04438122847357562 1108 10-11 0.17643178545274907 1108 12-13 0.18262476691608498 1108 14-15 0.6550898949250552 1108 16-17 0.7120323675234772 1108 18-19 0.6532434630762793 1108 20-21 0.5482532966747655 1108 22-23 0.6890725619786195 1108 24-25 0.4113116694134433 1108 26-27 0.4138148253344127 1108 28-29 0.31122260754926145 1108 30-31 0.46852231607005734 1108 32-33 0.41638788462508813 1108 34-35 0.412585104428544 1108 36-37 0.3463306813340594 1108 38-39 0.4639955006560683 1108 40-41 0.49066399171965003 1108 42-43 0.3923929392103602 1108 44-45 0.29602778384850836 1108 46-47 0.5151722298149366 1108 48-49 0.5159579357608379 1108 50-51 0.5153829620236294 1108 52-53 0.4434674147280191 1108 54-55 0.4747891582347208 1108 56-57 0.5558209141912727 1108 58-59 0.5490955684172221 1108 60-61 0.5380115703530706 1108 62-63 0.6929449299090038 1108 64-65 0.7114988425953257 1108 66-67 0.6636598173027508 1108 68-69 0.6953146413855436 1108 70-71 0.5432706841098138 1108 72-73 0.8057338228939912 1108 74-75 0.7368620446064611 1108 76-77 0.7876413032594485 1108 78-79 0.88269385369885 1108 80-81 0.8592406766370573 1108 82-83 0.9765623639479912 1108 84-85 0.906708710676245 1108 86-87 0.9640478219484407 1108 88-89 0.8772488275777341 1108 90-91 1.1080744505068516 1108 92-93 1.2595128039269206 1108 94-95 1.3253700773376984 1108 96-97 1.759770998932968 1108 98-99 1.878402375816858 1108 100-101 2.0084183315986515 1109 1 0.1066896405304334 1109 2 0.15788202482957558 1109 3 0.23867628664119422 1109 4 0.1036800874609014 1109 5 0.3298493070843449 1109 6 0.2924936300554677 1109 7 0.3095800390772965 1109 8 0.18814979016786282 1109 9 0.3951094001290585 1109 10-11 0.32485196512553216 1109 12-13 0.35262072197441796 1109 14-15 0.27285681760472613 1109 16-17 0.0825312559795961 1109 18-19 0.2385369593491049 1109 20-21 0.15455815660371997 1109 22-23 0.22520871458485914 1109 24-25 0.18129794139002087 1109 26-27 0.17101823395306326 1109 28-29 0.13082538167068236 1109 30-31 0.13424266345251823 1109 32-33 -0.0031884702238897944 1109 34-35 0.1467550045852093 1109 36-37 0.07489830467326186 1109 38-39 0.22367309424397774 1109 40-41 0.08634571762519272 1109 42-43 0.18474816246679637 1109 44-45 0.07553798075306162 1109 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-3.9623598377331355 2316 96-97 -5.534055178413649 2316 98-99 -7.326969842078018 2316 100-101 -8.181111266370838 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9420.0 3 3505.0 4 3676.0 5 3774.0 6 3918.0 7 4622.0 8 5671.0 9 6906.0 10 8636.0 11 10577.0 12 12603.0 13 14923.0 14 16340.0 15 19949.0 16 23427.0 17 28619.0 18 23500.0 19 18831.0 20 19229.0 21 20671.0 22 22927.0 23 25845.0 24 29599.0 25 34430.0 26 40359.0 27 47218.0 28 56427.0 29 67250.0 30 82073.0 31 100555.0 32 124962.0 33 157986.0 34 207909.0 35 279849.0 36 385710.0 37 499783.0 38 381614.0 39 53838.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.31642298964266 18.405948421005807 14.33719650993651 22.94043207941502 2 15.64115392196438 19.70580234435256 40.2586763136087 24.394367420074357 3 19.479016070934748 24.29046000469071 29.348341273702967 26.882182650671577 4 12.837369975726235 16.34999450392112 36.98423500533154 33.8284005150211 5 14.057669942747356 37.155754216229724 33.65964609366412 15.126929747358794 6 32.15818036493951 35.52521803488348 17.50790968344111 14.8086919167359 7 29.185563153846477 32.471170160152546 20.214757963844836 18.128508722156138 8 26.778864959680966 34.17538667018724 19.48438838926614 19.56135998086566 9 26.693095369933552 16.87426822857291 20.21830823377039 36.214328167723146 10-11 25.270062964641987 26.344413912631154 26.934487460389917 21.45103566233694 12-13 26.41158345134204 23.696685783231494 27.46124094452018 22.43048982090628 14-15 23.08445106440491 25.064451827707824 26.025530731870944 25.825566376016322 16-17 22.361959849550498 28.494167297858432 26.450436651633623 22.69343620095745 18-19 22.272128412241393 26.889692126818993 28.871779473545526 21.966399987394087 20-21 23.24446259319736 25.975451225119585 28.84032094354223 21.93976523814083 22-23 22.694849268306687 26.045681213722492 28.8755627804276 22.38390673754322 24-25 22.75479508114941 26.225923072156654 28.91561098273039 22.103670863963544 26-27 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26.858734270431157 27.61733060471055 22.797212856727352 52-53 23.00041440012432 26.95858308757493 27.367925710377712 22.673076801923038 54-55 22.72693784086723 26.65948583243573 27.55760344470043 23.05597288199661 56-57 22.820635368702607 27.071115450450822 27.362498339642542 22.74575084120403 58-59 22.98651208235381 26.706850467713334 27.409046115409364 22.897591334523494 60-61 22.860502002173945 26.577592487555652 27.957737075298226 22.60416843497218 62-63 22.750925334476694 26.776755947953763 27.773229614203338 22.699089103366205 64-65 22.614138636046647 26.83319983682918 27.633881360253838 22.918780166870338 66-67 22.55414557692486 27.070784707270622 27.39303053705363 22.982039178750885 68-69 22.471475550184078 26.8402573058765 27.65352154036317 23.034745603576255 70-71 22.61120123762073 26.789588725611154 27.564134199247835 23.035075837520278 72-73 22.74075452325195 26.693710987624737 27.64900821639284 22.916526272730472 74-75 22.496956301663236 27.185599438797336 27.289844794138173 23.027599465401252 76-77 22.650246714139456 26.998748618693142 27.31048242333517 23.040522243832235 78-79 22.78375704140485 26.980830785565495 27.297485056897475 22.93792711613218 80-81 22.54651115779481 27.31841706908166 27.288716155704197 22.84635561741933 82-83 22.634233725922844 27.19249089871135 27.284282570642837 22.888992804722974 84-85 22.6425568316255 27.076505161293028 27.117806705970803 23.163131301110663 86-87 22.783840078009362 27.016043929966578 27.151723797580267 23.048392194443792 88-89 22.77828829852041 26.966987031800905 27.018402722056546 23.236321947622145 90-91 22.66907665762862 27.032856719585315 27.273949153927614 23.024117468858456 92-93 22.810552562682464 26.861318442027393 27.354093863601875 22.974035131688264 94-95 22.508046182611636 26.861674617608333 27.297933571934507 23.332345627845523 96-97 22.592070455283313 26.85587872646335 27.21541839387647 23.33663242437687 98-99 22.58510805328352 26.839972477101153 26.946167737400316 23.628751732215004 100-101 22.491030549516022 26.737994028651137 27.157525219304773 23.613450202528075 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 216.0 1 192.5 2 262.5 3 376.0 4 330.0 5 345.0 6 646.0 7 1309.5 8 2239.5 9 2725.0 10 2488.5 11 2195.5 12 2260.0 13 2446.5 14 2685.5 15 2867.5 16 3068.5 17 3318.5 18 3475.5 19 3758.5 20 4186.5 21 4878.0 22 5856.0 23 7090.0 24 8636.0 25 10808.5 26 13799.0 27 17361.5 28 20639.5 29 24449.0 30 30186.0 31 36195.5 32 42206.5 33 47356.0 34 53215.5 35 58555.0 36 63814.0 37 69382.0 38 72061.0 39 74088.5 40 77511.0 41 84512.5 42 91726.5 43 97757.0 44 101991.5 45 105495.0 46 107020.0 47 109917.0 48 120732.5 49 120625.5 50 110295.0 51 103998.5 52 100356.0 53 99212.5 54 96426.0 55 90362.5 56 82908.5 57 74662.5 58 67987.5 59 60975.5 60 51799.5 61 42648.5 62 34820.0 63 27450.5 64 21078.5 65 16693.5 66 12937.5 67 10579.0 68 8022.0 69 5458.5 70 4176.0 71 3339.0 72 2736.5 73 2365.5 74 1914.5 75 1581.5 76 1169.0 77 689.5 78 416.5 79 278.0 80 191.5 81 137.5 82 93.5 83 61.0 84 46.5 85 34.0 86 21.5 87 16.0 88 12.0 89 10.0 90 7.0 91 4.0 92 5.0 93 3.5 94 3.0 95 3.0 96 2.5 97 1.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07234497106201157 2 0.029154988338004662 3 0.01518999392400243 4 0.019669992132003145 5 0.02386999045200382 6 0.02922498831000468 7 0.07405997037601185 8 0.054074978370008644 9 0.06702497319001073 10-11 0.05783747686500926 12-13 0.03743248502700599 14-15 0.039724984110006356 16-17 0.05022497991000804 18-19 0.04695248121900752 20-21 0.017657492937002826 22-23 0.012109995156001937 24-25 0.021804991278003488 26-27 0.0231174907530037 28-29 0.006037497585000966 30-31 8.924996430001429E-4 32-33 0.008084996766001294 34-35 0.010027495989001605 36-37 0.0035524985790005683 38-39 0.008242496703001319 40-41 0.0034474986210005516 42-43 0.0014524994190002324 44-45 1.7499993000002798E-4 46-47 3.324998670000532E-4 48-49 5.949997620000952E-4 50-51 8.749996500001401E-4 52-53 6.99999720000112E-5 54-55 5.24999790000084E-5 56-57 0.0027474989010004393 58-59 0.003692498523000591 60-61 0.004357498257000697 62-63 0.0024149990340003864 64-65 5.424997830000867E-4 66-67 2.974998810000476E-4 68-69 0.0027124989150004337 70-71 0.0017149993140002744 72-73 0.02094749162100335 74-75 0.014769994092002364 76-77 0.024517490193003925 78-79 0.01954749218100313 80-81 0.011532495387001846 82-83 0.004584998166000734 84-85 0.003779998488000605 86-87 0.0017849992860002857 88-89 0.0013824994470002213 90-91 0.005197497921000832 92-93 0.009257496297001481 94-95 0.012407495037001986 96-97 0.010377495849001661 98-99 0.021472491411003436 100-101 0.017604992958002816 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2857144.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.642867679711244 #Duplication Level Percentage of deduplicated Percentage of total 1 76.1236452770663 39.31243340340811 2 13.177099354724048 13.61006396756845 3 4.497053355196179 6.967221941129933 4 1.9907440084494905 4.112309176501401 5 1.0594707204845053 2.735705311425482 6 0.6747973113801649 2.090908095733846 7 0.43947848342658263 1.5887150417375424 8 0.30319858117147586 1.2526435366491775 9 0.21665947989876413 1.0070025166770242 >10 1.3106543282114722 12.788131342147297 >50 0.12862238885797045 4.651044609004007 >100 0.07352903278935177 7.0182808598902815 >500 0.0038880765620150164 1.3444218727738082 >1k 0.0010231780426101912 0.9127907655349219 >5k 1.3642373901469215E-4 0.6083275598187159 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9638 0.33732986506805396 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7631 0.2670848931660427 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4469 0.15641493743402504 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3218 0.11262995494801802 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 1.049999580000168E-4 7 0.0 0.0 0.0 0.0 1.049999580000168E-4 8 0.0 0.0 0.0 0.0 1.049999580000168E-4 9 0.0 0.0 0.0 3.49999860000056E-5 1.399999440000224E-4 10-11 0.0 0.0 0.0 3.49999860000056E-5 1.399999440000224E-4 12-13 0.0 0.0 0.0 3.49999860000056E-5 1.399999440000224E-4 14-15 0.0 0.0 0.0 3.49999860000056E-5 1.74999930000028E-4 16-17 0.0 0.0 0.0 3.49999860000056E-5 2.099999160000336E-4 18-19 0.0 0.0 0.0 6.99999720000112E-5 2.449999020000392E-4 20-21 0.0 0.0 0.0 6.99999720000112E-5 2.449999020000392E-4 22-23 0.0 0.0 0.0 1.049999580000168E-4 2.62499895000042E-4 24-25 0.0 0.0 0.0 1.049999580000168E-4 2.799998880000448E-4 26-27 0.0 0.0 0.0 1.5749993700002522E-4 2.799998880000448E-4 28-29 0.0 0.0 0.0 4.724998110000756E-4 2.974998810000476E-4 30-31 0.0 0.0 0.0 0.0010324995870001651 3.49999860000056E-4 32-33 0.0 0.0 0.0 0.002432499027000389 3.849998460000616E-4 34-35 0.0 0.0 0.0 0.003727498509000596 3.849998460000616E-4 36-37 0.0 0.0 0.0 0.005669997732000907 3.849998460000616E-4 38-39 1.74999930000028E-5 0.0 0.0 0.007874996850001259 3.849998460000616E-4 40-41 3.49999860000056E-5 0.0 0.0 0.009694996122001552 3.849998460000616E-4 42-43 3.49999860000056E-5 0.0 0.0 0.012582494967002013 3.849998460000616E-4 44-45 3.49999860000056E-5 0.0 0.0 0.016029993588002566 4.0249983900006437E-4 46-47 3.49999860000056E-5 0.0 0.0 0.021122491551003382 4.724998110000756E-4 48-49 3.49999860000056E-5 1.74999930000028E-5 0.0 0.027597488961004415 5.24999790000084E-4 50-51 3.49999860000056E-5 3.49999860000056E-5 0.0 0.03470248611900555 5.24999790000084E-4 52-53 3.49999860000056E-5 3.49999860000056E-5 0.0 0.0456049817580073 5.24999790000084E-4 54-55 3.49999860000056E-5 3.49999860000056E-5 0.0 0.06546747381301048 5.24999790000084E-4 56-57 3.49999860000056E-5 3.49999860000056E-5 0.0 0.0975799609680156 5.24999790000084E-4 58-59 3.49999860000056E-5 3.49999860000056E-5 0.0 0.13457494617002153 5.24999790000084E-4 60-61 3.49999860000056E-5 3.49999860000056E-5 0.0 0.1718674312530275 5.24999790000084E-4 62-63 3.49999860000056E-5 3.49999860000056E-5 0.0 0.21638741344503462 5.24999790000084E-4 64-65 3.49999860000056E-5 3.49999860000056E-5 0.0 0.26944739222104314 5.424997830000867E-4 66-67 3.49999860000056E-5 3.49999860000056E-5 0.0 0.34611486155405535 5.774997690000924E-4 68-69 3.49999860000056E-5 3.49999860000056E-5 0.0 0.4429073228370709 5.949997620000952E-4 70-71 6.99999720000112E-5 3.49999860000056E-5 0.0 0.5360072855970858 6.649997340001063E-4 72-73 6.99999720000112E-5 3.49999860000056E-5 0.0 0.6280397487841005 7.699996920001232E-4 74-75 6.99999720000112E-5 3.49999860000056E-5 0.0 0.730572207771117 7.699996920001232E-4 76-77 6.99999720000112E-5 3.49999860000056E-5 0.0 0.849344660262136 7.699996920001232E-4 78-79 6.99999720000112E-5 3.49999860000056E-5 0.0 0.9618346152661539 7.699996920001232E-4 80-81 6.99999720000112E-5 3.49999860000056E-5 0.0 1.087222065111174 8.224996710001315E-4 82-83 6.99999720000112E-5 3.49999860000056E-5 0.0 1.2305645077741967 8.7499965000014E-4 84-85 6.99999720000112E-5 3.49999860000056E-5 0.0 1.4046719381312247 8.7499965000014E-4 86-87 6.99999720000112E-5 3.49999860000056E-5 0.0 1.6213393514642593 8.7499965000014E-4 88-89 6.99999720000112E-5 3.49999860000056E-5 0.0 1.8782217487113004 8.924996430001428E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 16115 0.0 30.460997 1 GGTATCA 11035 0.0 27.517097 1 ATCAACG 18920 0.0 25.544014 3 TATCAAC 19050 0.0 25.518032 2 TCAACGC 19075 0.0 25.412968 4 CAACGCA 19380 0.0 24.965286 5 AACGCAG 19830 0.0 24.470655 6 GTACATG 13225 0.0 24.110209 1 TACATGG 13470 0.0 23.38904 2 GAGTACT 9525 0.0 23.051573 12-13 ACGCAGA 21920 0.0 22.094093 7 CGCAGAG 22220 0.0 21.820623 8 ACATGGG 13685 0.0 21.807415 3 GTACTTT 10215 0.0 21.32941 14-15 GCAGAGT 23145 0.0 20.394747 9 AGTACTT 10155 0.0 20.006897 12-13 GTGTAGC 1735 0.0 19.72001 1 AGAGTAC 18400 0.0 18.702736 10-11 GTACCGT 180 1.2287237E-4 18.482815 6 ACTTTTT 12410 0.0 17.842743 16-17 >>END_MODULE