FastQCFastQC Report
Thu 26 May 2016
SRR936404_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936404_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2857144
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102230.35780485687805724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93440.3270398691840523No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57500.20124991950003218No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38110.13338494664602132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131700.028.1008471
GTATCAA180950.026.3073351
GTACATG129350.024.3876171
TACATGG133900.022.9158522
GAGTACT114850.022.84635712-13
ACATGGG131550.022.1309723
ATCAACG216400.021.9249743
TCAACGC217750.021.7660924
GTACTTT120350.021.72335614-15
TATCAAC220550.021.5796682
CAACGCA221500.021.397595
AACGCAG225700.021.0200826
AGTACTT120950.020.02534312-13
ACGCAGA249400.019.0416267
CGCAGAG252100.018.8188518
TAAGGTG10300.018.4429685
ACTTTTT144450.018.34577816-17
AGAGTAC204000.018.25098610-11
TACACCG2352.5348527E-618.1879085
GCAGAGT262450.017.8052889
Produced by FastQC (version 0.11.5)