##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936404_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2857144 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.770503341798662 31.0 31.0 34.0 30.0 34.0 2 31.93527767588893 31.0 31.0 34.0 30.0 34.0 3 32.01379244448302 31.0 31.0 34.0 30.0 34.0 4 35.733137006745196 37.0 35.0 37.0 35.0 37.0 5 35.55103767958493 37.0 35.0 37.0 33.0 37.0 6 35.641090543563784 37.0 35.0 37.0 33.0 37.0 7 35.577288719084514 37.0 35.0 37.0 33.0 37.0 8 35.54202203319119 37.0 35.0 37.0 33.0 37.0 9 37.144123292350685 39.0 37.0 39.0 33.0 39.0 10-11 37.0299617380153 39.0 37.0 39.0 33.0 39.0 12-13 36.91626095849561 39.0 37.0 39.0 33.0 39.0 14-15 38.01624454350218 40.0 37.0 41.0 33.0 41.0 16-17 37.904173363330656 40.0 37.0 41.0 33.0 41.0 18-19 38.056817052273175 40.0 37.0 41.0 33.0 41.0 20-21 38.07384909546036 40.0 37.0 41.0 33.0 41.0 22-23 37.98836180465528 40.0 37.0 41.0 33.0 41.0 24-25 37.851617209353115 40.0 37.0 41.0 32.5 41.0 26-27 37.64711491615403 39.0 37.0 41.0 32.0 41.0 28-29 37.558162101735164 39.0 36.5 41.0 32.0 41.0 30-31 37.5337510114996 39.0 36.5 41.0 32.0 41.0 32-33 37.378096273761486 39.0 36.0 41.0 32.0 41.0 34-35 37.206553292378686 39.0 36.0 41.0 31.0 41.0 36-37 37.00972719610912 39.0 36.0 41.0 31.0 41.0 38-39 36.839169464332215 39.0 35.0 41.0 30.5 41.0 40-41 36.642919992832006 39.0 35.0 40.5 30.0 41.0 42-43 36.358940431423825 39.0 35.0 40.0 30.0 41.0 44-45 36.08840226463909 38.5 35.0 40.0 29.5 41.0 46-47 35.72591860963256 38.0 35.0 40.0 28.5 41.0 48-49 35.34843973562411 38.0 34.5 40.0 27.0 41.0 50-51 35.05569005272398 38.0 34.0 40.0 26.5 41.0 52-53 34.6865462503815 38.0 34.0 40.0 26.0 41.0 54-55 34.4870115051954 38.0 33.0 40.0 25.0 41.0 56-57 34.17455648017741 37.5 33.0 40.0 24.5 41.0 58-59 33.78723648510541 37.0 33.0 40.0 23.0 41.0 60-61 33.37269280092288 36.5 32.0 39.0 22.5 41.0 62-63 33.03069463772214 36.0 32.0 39.0 22.0 40.0 64-65 32.596775836289666 35.5 31.0 39.0 21.5 40.0 66-67 32.16426613429354 35.0 31.0 38.0 20.0 40.0 68-69 31.789496434201425 35.0 31.0 38.0 19.0 40.0 70-71 31.409992986002806 35.0 30.0 37.5 18.0 39.5 72-73 30.94150644839742 34.0 30.0 37.0 17.0 39.0 74-75 30.42375060549976 34.0 29.0 36.0 12.5 39.0 76-77 28.959489091204365 32.5 28.0 35.0 11.0 37.0 78-79 29.730141882943247 34.0 29.0 35.0 9.0 37.0 80-81 29.51004394598242 34.0 29.0 35.0 7.0 37.0 82-83 29.202804968878013 34.0 29.0 35.0 6.0 36.5 84-85 28.80820655171738 33.5 29.0 35.0 2.0 36.0 86-87 28.392334793066084 33.0 28.0 35.0 2.0 36.0 88-89 27.903586763565293 33.0 27.0 35.0 2.0 35.0 90-91 27.409689186124325 33.0 26.0 35.0 2.0 35.0 92-93 26.75591114763554 32.0 25.0 34.0 2.0 35.0 94-95 26.16662530834988 32.0 24.5 34.0 2.0 35.0 96-97 25.38854639458144 32.0 21.5 34.0 2.0 35.0 98-99 24.41531543387383 31.0 10.0 34.0 2.0 35.0 100-101 21.12176092629563 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3458925782866693 1101 2 0.2752518692621102 1101 3 0.3443079113209784 1101 4 0.2874988059005972 1101 5 0.23902724285287746 1101 6 0.23363294673401924 1101 7 0.28955794969997584 1101 8 0.22295905885716394 1101 9 0.2922186829245632 1101 10-11 0.37628782775360037 1101 12-13 0.3935026683240679 1101 14-15 0.5337386088044767 1101 16-17 0.4969147280850592 1101 18-19 0.511843471043143 1101 20-21 0.5268020933883335 1101 22-23 0.4059889573856381 1101 24-25 0.4609739829670403 1101 26-27 0.4895440948268046 1101 28-29 0.3362228687180746 1101 30-31 0.25970612813282656 1101 32-33 0.3086400457292058 1101 34-35 0.3412800672922103 1101 36-37 0.3784880735983336 1101 38-39 0.34977721507199533 1101 40-41 0.4258262361138705 1101 42-43 0.5035026857063158 1101 44-45 0.49800742443699875 1101 46-47 0.494735412068799 1101 48-49 0.3433240212355244 1101 50-51 0.33669610380785286 1101 52-53 0.40638293544324 1101 54-55 0.25783449288870486 1101 56-57 0.2829401673015184 1101 58-59 0.3222552481283145 1101 60-61 0.43608955482442724 1101 62-63 0.39040003991830474 1101 64-65 0.44147973885510083 1101 66-67 0.265929733774648 1101 68-69 0.17646679189128633 1101 70-71 0.23070752694174246 1101 72-73 0.5150085284144765 1101 74-75 0.41415628044300234 1101 76-77 0.24869435993019806 1101 78-79 0.4641640667946554 1101 80-81 0.456131450488261 1101 82-83 0.5378670128795342 1101 84-85 0.5621745847661295 1101 86-87 0.4249543110418301 1101 88-89 0.4016769451318254 1101 90-91 0.500817805180894 1101 92-93 0.5539175540320471 1101 94-95 0.5145869542091859 1101 96-97 0.6596058166358532 1101 98-99 0.4768690156595419 1101 100-101 1.0032754318857435 1102 1 0.27266454971620036 1102 2 0.22901799372025877 1102 3 0.21716282060648595 1102 4 0.18828806695762523 1102 5 0.163704090955882 1102 6 0.19954091174828648 1102 7 0.20868992269797815 1102 8 0.24044065832167405 1102 9 0.2952991813668646 1102 10-11 0.30115847034608123 1102 12-13 0.2468453609353034 1102 14-15 0.4399704630074055 1102 16-17 0.43520330569756993 1102 18-19 0.3521565908030624 1102 20-21 0.283009564500027 1102 22-23 0.2677355046791732 1102 24-25 0.3293209750655919 1102 26-27 0.3780399701355961 1102 28-29 0.406628036718395 1102 30-31 0.4637765856015932 1102 32-33 0.4428318221934191 1102 34-35 0.43946135930333696 1102 36-37 0.3990463607842045 1102 38-39 0.41419480160435285 1102 40-41 0.46208756541483353 1102 42-43 0.5068400488310161 1102 44-45 0.4498048973215063 1102 46-47 0.40610262694540467 1102 48-49 0.5534534145411385 1102 50-51 0.5069696638661867 1102 52-53 0.4606505286857825 1102 54-55 0.5444777503150533 1102 56-57 0.3097841006061728 1102 58-59 0.56242582483398 1102 60-61 0.6909922129955817 1102 62-63 0.6700567547123555 1102 64-65 0.4876455736984511 1102 66-67 0.277288443563954 1102 68-69 0.3830923101355239 1102 70-71 0.4948305834050295 1102 72-73 0.4995055215347435 1102 74-75 0.40439995119450245 1102 76-77 0.40493424978379267 1102 78-79 0.47364309706006225 1102 80-81 0.5248474197837893 1102 82-83 0.5377002800315047 1102 84-85 0.5751666026864584 1102 86-87 0.5267420914404752 1102 88-89 0.45550000949269176 1102 90-91 0.4941135872650939 1102 92-93 0.4622739680000514 1102 94-95 0.427116494199943 1102 96-97 0.7654303655210519 1102 98-99 0.7135422341889246 1102 100-101 1.2306953915583705 1103 1 0.18438138861590758 1103 2 0.19800606221409467 1103 3 0.15871663799598323 1103 4 0.17307294072586643 1103 5 0.25808822565462464 1103 6 0.2223766990736351 1103 7 0.12615366035621634 1103 8 0.10845716821521023 1103 9 0.23786648162136004 1103 10-11 0.2070362952014193 1103 12-13 0.22309496974057907 1103 14-15 0.25657225710826737 1103 16-17 0.2767333964225429 1103 18-19 0.19679551874432377 1103 20-21 0.16160365763416706 1103 22-23 0.05481885881359716 1103 24-25 0.18767804848351233 1103 26-27 0.10941719565109764 1103 28-29 0.08311770354126935 1103 30-31 0.2866218743287945 1103 32-33 0.2305664786372219 1103 34-35 0.20436688477111886 1103 36-37 0.18621120151762938 1103 38-39 0.22996015026017602 1103 40-41 0.30513777307682943 1103 42-43 0.40209208559338805 1103 44-45 0.3553065847597381 1103 46-47 0.3700327825160983 1103 48-49 0.20831934009846265 1103 50-51 0.35907937768074305 1103 52-53 0.3297693085554769 1103 54-55 0.34599749149042935 1103 56-57 0.13416040335840762 1103 58-59 0.2851341752778893 1103 60-61 0.26042561273136755 1103 62-63 0.42733388913249115 1103 64-65 0.43209146750928795 1103 66-67 0.13251579815358383 1103 68-69 0.21702326554174434 1103 70-71 0.2043443853990361 1103 72-73 0.2111734040587656 1103 74-75 0.07029270989163905 1103 76-77 0.3141640296465127 1103 78-79 0.09294017583527037 1103 80-81 0.32146314927404873 1103 82-83 0.4252738420855877 1103 84-85 0.7533108017585413 1103 86-87 0.7461916757675624 1103 88-89 0.6152917409111431 1103 90-91 0.576137579109993 1103 92-93 0.600870380120405 1103 94-95 0.6502884808464451 1103 96-97 0.6573140748039208 1103 98-99 0.655448086569347 1103 100-101 1.1508484553847111 1104 1 0.2209770663537043 1104 2 0.2734195088150777 1104 3 0.23624465518404847 1104 4 0.2725088226448449 1104 5 0.1804927570374062 1104 6 0.1471103135039229 1104 7 0.2351788518791622 1104 8 0.19338665821263135 1104 9 0.22887683425278027 1104 10-11 0.2868775898121356 1104 12-13 0.24724781467904933 1104 14-15 0.3392155348332935 1104 16-17 0.41979433593425597 1104 18-19 0.2816213471017974 1104 20-21 0.3431886668394881 1104 22-23 0.3376028964646025 1104 24-25 0.3298458380367535 1104 26-27 0.33340742766920783 1104 28-29 0.3828695816618293 1104 30-31 0.32024080843128644 1104 32-33 0.2711109195082244 1104 34-35 0.12731462217741552 1104 36-37 0.1634088652876855 1104 38-39 0.34045965240942166 1104 40-41 0.30731403317630424 1104 42-43 0.2581840914572169 1104 44-45 0.21449913617665572 1104 46-47 0.1561281778671102 1104 48-49 0.33788317462974504 1104 50-51 0.24398498479752817 1104 52-53 0.40222112994494097 1104 54-55 0.2049265805853011 1104 56-57 0.2010169806868234 1104 58-59 0.39872502505805585 1104 60-61 0.5198159511916458 1104 62-63 0.4320660086361343 1104 64-65 0.2963518097269642 1104 66-67 0.35918770023525326 1104 68-69 0.3488775412751508 1104 70-71 0.3977240381996765 1104 72-73 0.5083625507643532 1104 74-75 0.42203431002138103 1104 76-77 0.3375079331896984 1104 78-79 0.41428771794959474 1104 80-81 0.6156759752373091 1104 82-83 0.4974549591108577 1104 84-85 0.5928218084996679 1104 86-87 0.5761901808409036 1104 88-89 0.5517165487432898 1104 90-91 0.6658306412758925 1104 92-93 0.7258639045717565 1104 94-95 0.6961978476931989 1104 96-97 0.9996077701674686 1104 98-99 1.138374522856612 1104 100-101 1.6242055108993512 1105 1 -0.05084588411136437 1105 2 0.027434966642996272 1105 3 -6.026717265505965E-4 1105 4 0.10087202336365664 1105 5 0.0610317427110445 1105 6 -0.013854095221674356 1105 7 -0.011745805769052708 1105 8 -0.03204117752506619 1105 9 -0.03023043042716722 1105 10-11 0.0557570636802609 1105 12-13 0.07417713130661241 1105 14-15 0.08608497109678837 1105 16-17 0.05952690219992007 1105 18-19 0.05784318523554077 1105 20-21 0.036379173337103055 1105 22-23 0.08869609269083156 1105 24-25 0.06513656666783874 1105 26-27 -0.07148190077219141 1105 28-29 -0.048462678442334095 1105 30-31 0.08381840539629337 1105 32-33 -0.0026347958946999483 1105 34-35 0.011893296531404474 1105 36-37 0.008508617363432336 1105 38-39 -0.041106596613026625 1105 40-41 0.1350211289682477 1105 42-43 0.08194301423463912 1105 44-45 0.11910351811312836 1105 46-47 0.15161676752750708 1105 48-49 0.22715816256631882 1105 50-51 0.24272664439253333 1105 52-53 0.4142767589903471 1105 54-55 0.20919256254840235 1105 56-57 0.011074864345452795 1105 58-59 0.1511212795714414 1105 60-61 0.31245944359584854 1105 62-63 0.22485124632726894 1105 64-65 0.2510305481419195 1105 66-67 0.13064801987451347 1105 68-69 0.17454591762084704 1105 70-71 0.23801796987907053 1105 72-73 0.2470285435429389 1105 74-75 0.0855897560354606 1105 76-77 0.03580459785965928 1105 78-79 0.05266772427249933 1105 80-81 0.07525901738442897 1105 82-83 0.2973663553071333 1105 84-85 0.471740758898175 1105 86-87 0.6477282887961096 1105 88-89 0.4318628490145109 1105 90-91 0.46000431721060053 1105 92-93 0.240200561587681 1105 94-95 0.569916836361898 1105 96-97 0.7208719550956708 1105 98-99 0.5541744945699492 1105 100-101 1.0187160268813145 1106 1 0.11929907863564182 1106 2 0.10141331256011554 1106 3 0.060935478332009296 1106 4 0.14003875232630492 1106 5 0.15149516251120332 1106 6 0.03831754266424525 1106 7 -0.001944779131292762 1106 8 0.0568150059739736 1106 9 0.08965911879911914 1106 10-11 0.09040017000704381 1106 12-13 0.07921376134324021 1106 14-15 0.15937105292878329 1106 16-17 0.10135647285602545 1106 18-19 0.15913878589338282 1106 20-21 0.1029858801223753 1106 22-23 0.05574650259838165 1106 24-25 0.0973090144015103 1106 26-27 0.023126849321251086 1106 28-29 -0.17370282944779092 1106 30-31 -0.07388386965291716 1106 32-33 -0.09043864454037731 1106 34-35 0.03530677364386037 1106 36-37 -0.0780105324210254 1106 38-39 0.17177745810776202 1106 40-41 0.18063876246853994 1106 42-43 0.17802740037004838 1106 44-45 -0.013035445530938716 1106 46-47 0.13985980283685961 1106 48-49 0.01889428925142056 1106 50-51 0.15757738322285775 1106 52-53 -0.20006206261887627 1106 54-55 0.007729074222659449 1106 56-57 -0.12896696740556024 1106 58-59 -0.08320785501278749 1106 60-61 -0.16813489120767855 1106 62-63 -0.06148973695248827 1106 64-65 -0.08144652693719934 1106 66-67 -0.0063581287387748375 1106 68-69 -0.19631685056334902 1106 70-71 0.055529993003336386 1106 72-73 -0.014341407367830072 1106 74-75 -0.06502121179489606 1106 76-77 -0.2174941300564157 1106 78-79 -0.3304463272089002 1106 80-81 -0.09663630667926171 1106 82-83 -0.06498756061820998 1106 84-85 0.23268367086149766 1106 86-87 0.3622240962766128 1106 88-89 0.23070383229427094 1106 90-91 0.39336895427421936 1106 92-93 0.3406760356600955 1106 94-95 0.38470496773166474 1106 96-97 0.7565786095523244 1106 98-99 0.8066734978342573 1106 100-101 1.1942178210055587 1107 1 0.05446612220775293 1107 2 0.047726010512239014 1107 3 0.05849472771445008 1107 4 0.05863116785265987 1107 5 0.08843634837394632 1107 6 0.04480430798648882 1107 7 0.07770309446183887 1107 8 0.08542297364760998 1107 9 0.033837196258943436 1107 10-11 0.07413566375316805 1107 12-13 0.051838451029546206 1107 14-15 0.07858785723119155 1107 16-17 0.20899068880825666 1107 18-19 -0.0016711846757502258 1107 20-21 -0.030207744878964604 1107 22-23 0.07949691903667855 1107 24-25 0.23301692880176006 1107 26-27 0.05417203652273628 1107 28-29 0.06327781250431741 1107 30-31 -0.0030147456548661467 1107 32-33 -0.0015646476385242636 1107 34-35 0.15342249205941272 1107 36-37 0.19480099796860628 1107 38-39 0.11597810383660345 1107 40-41 0.08141422047390279 1107 42-43 0.17607808532468283 1107 44-45 0.15171000128240308 1107 46-47 0.045682723293694494 1107 48-49 0.1616018981356433 1107 50-51 0.0625984392643204 1107 52-53 0.2876491310890188 1107 54-55 0.1926269105858296 1107 56-57 0.05913279969604446 1107 58-59 0.07390199411756271 1107 60-61 0.1628521097764306 1107 62-63 0.17520365852802655 1107 64-65 0.2921579633807099 1107 66-67 0.28177740983684174 1107 68-69 0.37729761892111924 1107 70-71 0.2531868846925356 1107 72-73 0.38920104323913307 1107 74-75 0.5055823128467374 1107 76-77 0.4008770550233507 1107 78-79 0.40885316600296306 1107 80-81 0.5651947629185194 1107 82-83 0.4923627096636771 1107 84-85 0.7686541141053702 1107 86-87 0.6092325724271745 1107 88-89 0.4714195995309787 1107 90-91 0.5429297941822853 1107 92-93 0.734728298858311 1107 94-95 0.7034005524877927 1107 96-97 1.053404006377722 1107 98-99 0.9973465081803532 1107 100-101 1.4196319947996514 1108 1 0.0722125801120228 1108 2 0.13929394100197 1108 3 0.04584315992870458 1108 4 0.11336698913087417 1108 5 0.15080721592892843 1108 6 0.1624850106985818 1108 7 0.10553335921953533 1108 8 0.12489935140499142 1108 9 0.06957659064948984 1108 10-11 0.036365199000435666 1108 12-13 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-1.241358762061683 2316 80-81 -1.0813114954360792 2316 82-83 -1.456331869544936 2316 84-85 -1.3289495764075419 2316 86-87 -1.3626073691357021 2316 88-89 -1.628094181134827 2316 90-91 -1.5652439921723236 2316 92-93 -1.7618228957242366 2316 94-95 -1.8464715829111356 2316 96-97 -1.9790981068936198 2316 98-99 -2.4580226032040713 2316 100-101 -3.1960099522677403 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 20.0 4 47.0 5 107.0 6 255.0 7 558.0 8 1299.0 9 2402.0 10 3789.0 11 5891.0 12 8267.0 13 11169.0 14 14469.0 15 19169.0 16 23834.0 17 29287.0 18 26559.0 19 19636.0 20 18894.0 21 19688.0 22 21479.0 23 24256.0 24 28578.0 25 34585.0 26 41997.0 27 52084.0 28 64005.0 29 78715.0 30 95936.0 31 116950.0 32 143742.0 33 180352.0 34 230582.0 35 304758.0 36 418726.0 37 518415.0 38 283306.0 39 13336.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.52343989853534 19.606477859216028 15.15700172340097 22.713080518847665 2 16.035302708155832 20.65442256976216 38.922800441616396 24.387474280465614 3 19.59377675538048 24.383440524036175 29.772529824264186 26.25025289631916 4 13.462604614958154 17.088918164432734 36.38924044430382 33.05923677630529 5 13.97620976845038 37.46165194579733 33.68157070829928 14.880567577453007 6 32.34635706145718 36.05695057721977 16.8788132484747 14.717879112848356 7 29.04389838244065 32.73926690429324 19.9994470002212 18.217387713044914 8 26.94596422161431 34.53949118420353 19.107507356997058 19.407037237185104 9 26.51952439219024 16.705213317914673 20.040991983603206 36.734270306291876 10-11 25.397884285476035 26.59967901507222 26.681946496562038 21.320490202889705 12-13 26.423878760281568 23.82207725866271 27.42597300460097 22.328070976454757 14-15 22.99841939952582 25.29330008799003 26.022542806762843 25.685737705721312 16-17 22.257167782145974 28.725081090635136 26.52277581534698 22.494975311871915 18-19 22.192368734537432 26.952210133241678 29.10461878935698 21.750802342863913 20-21 23.046895781241687 25.988172104731156 29.21301831479267 21.75191379923448 22-23 22.611397197408177 26.034994422746376 29.177926465652206 22.175681914193245 24-25 22.74241340303464 26.2288845084462 29.23725580509768 21.791446283421486 26-27 22.43302143667533 26.38081516172315 29.20328537135431 21.982878030247218 28-29 22.025927824415877 26.586511630104724 29.392976278124976 21.994584267354416 30-31 22.28116901196722 26.128046427553286 29.371496552531568 22.21928800794793 32-33 22.099080279850302 26.420585406285575 29.26361134018165 22.21672297368248 34-35 22.842714570019943 26.319421256483004 28.81883849488605 22.019025678611005 36-37 22.822988679541133 25.997995189574986 28.9497180385338 22.22929809235008 38-39 22.449458883985518 26.242529805962644 29.119305926682067 22.188705383369772 40-41 22.631018670590404 26.16602158696781 29.05539647070209 22.1475632717397 42-43 22.624210759536425 26.2652785998273 28.64825190171994 22.462258738916333 44-45 22.6228710070554 26.49264884487848 28.418691387286838 22.46578876077928 46-47 22.911184420641696 26.745088925479486 27.935737824491945 22.407988829386873 48-49 23.05585840361061 26.78585366109757 27.693966158406937 22.464321776884884 50-51 22.912688334924667 26.830079267968294 27.671758931296424 22.58547346581061 52-53 23.17866006612904 26.991790044663528 27.38867280263732 22.440877086570108 54-55 22.840502069673256 26.764699860190085 27.603366848162302 22.79143122197436 56-57 22.97944987296629 27.169112010199104 27.395402842838752 22.45603527399586 58-59 23.13196681137096 26.781613219437055 27.377415628063297 22.709004341128693 60-61 22.97415139069565 26.765832702036995 27.875665661726917 22.384350245540436 62-63 22.8684974323063 26.863409126431065 27.716354951340627 22.551738489922016 64-65 22.77688278755266 26.87942696354671 27.606189364995682 22.73750088390495 66-67 22.70771604360327 27.16896747185369 27.355504108303602 22.767812376239444 68-69 22.68234207053932 26.850213325959967 27.62590431656942 22.84154028693129 70-71 22.7638843291653 26.87428556228567 27.52477825743348 22.837051851115557 72-73 22.893140842743662 26.792541782983285 27.608968956412415 22.705348417860634 74-75 22.75484427022037 27.24602198898363 27.156211034742245 22.842922706053752 76-77 22.808799505091276 27.121295512833417 27.22776738940024 22.842137592675073 78-79 22.918307788565453 27.02996679107253 27.242223904302282 22.809501516059733 80-81 22.7191300520089 27.365223608523404 27.146371409893682 22.769274929574014 82-83 22.84838744795975 27.25039845163079 27.117009482676234 22.784204617733224 84-85 22.75542004317094 27.163732908730974 27.0316398428117 23.049207205286386 86-87 22.891221499297735 27.06401746686677 27.028033621093257 23.016727412742235 88-89 22.885577800785924 27.098175660794688 26.88157849126381 23.134668047155575 90-91 22.777019101143267 27.157816411926394 27.090621006005218 22.97454348092512 92-93 22.896760767915485 27.028633685717047 27.12350981460294 22.95109573176453 94-95 22.61156341955688 26.94228793191275 27.085653468518224 23.360495180012148 96-97 22.742923055347056 26.9779981297281 26.933106001275682 23.34597281364917 98-99 22.680153884835704 27.019615904234495 26.699110042505332 23.601120168424472 100-101 22.426080916175163 27.1352733636346 26.80574406049653 23.63290165969371 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 520.0 1 414.5 2 361.5 3 434.5 4 396.5 5 390.5 6 660.0 7 1378.5 8 2230.5 9 2723.5 10 2609.0 11 2309.0 12 2377.5 13 2631.5 14 2972.0 15 3281.5 16 3547.5 17 3845.0 18 4092.5 19 4347.5 20 4695.0 21 5325.5 22 6288.0 23 7501.5 24 9048.0 25 11234.5 26 14307.0 27 17875.0 28 21138.5 29 25133.0 30 30474.5 31 35873.5 32 41999.0 33 47329.0 34 53122.5 35 58424.0 36 63871.0 37 69525.0 38 72077.5 39 73636.5 40 77134.5 41 84816.0 42 91932.0 43 97647.0 44 102101.5 45 105134.5 46 106510.0 47 110019.5 48 121251.5 49 120938.5 50 109910.0 51 103824.0 52 100032.0 53 98275.5 54 95803.5 55 89943.5 56 82486.0 57 74206.0 58 67327.5 59 60575.0 60 51366.0 61 42024.5 62 34228.5 63 26927.5 64 20712.5 65 16628.0 66 12881.0 67 10252.5 68 7663.0 69 5193.0 70 4011.0 71 3294.0 72 2770.5 73 2432.5 74 1970.5 75 1591.5 76 1145.5 77 686.0 78 430.5 79 289.5 80 217.5 81 160.0 82 99.5 83 65.0 84 39.0 85 19.0 86 9.5 87 8.0 88 5.5 89 5.0 90 4.0 91 2.0 92 1.0 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020404991838003265 2 0.01280999487600205 3 0.008469996612001355 4 0.0 5 0.0031149987540004987 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.74999930000028E-5 12-13 9.62499615000154E-4 14-15 6.99999720000112E-5 16-17 5.24999790000084E-5 18-19 0.001487499405000238 20-21 0.0 22-23 1.049999580000168E-4 24-25 0.0 26-27 0.001907499237000305 28-29 0.0034124986350005456 30-31 0.0016624993350002658 32-33 0.006579997368001053 34-35 0.0020299991880003247 36-37 5.599997760000896E-4 38-39 0.0013824994470002213 40-41 1.224999510000196E-4 42-43 0.0043749982500007 44-45 0.0014699994120002352 46-47 1.574999370000252E-4 48-49 3.149998740000504E-4 50-51 0.0 52-53 1.049999580000168E-4 54-55 0.0017674992930002827 56-57 0.014769994092002364 58-59 0.026039989584004167 60-61 0.020579991768003292 62-63 0.013912494435002228 64-65 0.01756999297200281 66-67 0.002257499097000361 68-69 4.899998040000784E-4 70-71 6.99999720000112E-5 72-73 0.0 74-75 0.0011024995590001763 76-77 0.0018024992790002885 78-79 0.008102496759001297 80-81 0.01475249409900236 82-83 0.016134993546002584 84-85 0.028857488457004615 86-87 0.010727495709001717 88-89 0.040284983886006445 90-91 0.04528998188400724 92-93 0.027579988968004412 94-95 0.02831498867400453 96-97 0.04933248026700789 98-99 0.052569978972008415 100-101 0.059762476095009565 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2857144.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.109425539590966 #Duplication Level Percentage of deduplicated Percentage of total 1 74.14326737098136 36.41133268217194 2 14.224766545872 13.97140227005131 3 4.819185020730974 7.100022238112999 4 2.2139521693107036 4.349036768279195 5 1.184444508632908 2.9083694701242604 6 0.7100831826741133 2.092306631187008 7 0.4655233558317973 1.6003109206113513 8 0.33425562130807474 1.313208123665489 9 0.2513419103414316 1.1108931147801977 >10 1.4201329757108128 13.231070415703028 >50 0.1426138285239683 4.88567844521872 >100 0.08386253752651109 7.517603655922538 >500 0.005071076646039 1.647752422338446 >1k 0.0012856250651707 0.9730006640493042 >5k 1.4284722946341115E-4 0.5294330217433381 >10k+ 7.142361473170557E-5 0.35857915604095303 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10223 0.35780485687805724 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9344 0.3270398691840523 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5750 0.20124991950003218 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3811 0.13338494664602132 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.49999860000056E-5 2 0.0 0.0 0.0 0.0 3.49999860000056E-5 3 0.0 0.0 0.0 0.0 3.49999860000056E-5 4 0.0 0.0 0.0 0.0 3.49999860000056E-5 5 0.0 0.0 0.0 0.0 3.49999860000056E-5 6 0.0 0.0 0.0 0.0 3.49999860000056E-5 7 0.0 0.0 0.0 0.0 3.49999860000056E-5 8 0.0 0.0 0.0 0.0 3.49999860000056E-5 9 0.0 0.0 0.0 0.0 3.49999860000056E-5 10-11 0.0 0.0 0.0 0.0 3.49999860000056E-5 12-13 0.0 0.0 0.0 0.0 3.49999860000056E-5 14-15 0.0 0.0 0.0 0.0 3.49999860000056E-5 16-17 0.0 0.0 0.0 0.0 3.49999860000056E-5 18-19 0.0 0.0 0.0 0.0 3.49999860000056E-5 20-21 0.0 0.0 0.0 0.0 3.49999860000056E-5 22-23 0.0 0.0 0.0 1.049999580000168E-4 5.249997900000841E-5 24-25 0.0 0.0 0.0 1.224999510000196E-4 1.049999580000168E-4 26-27 0.0 0.0 0.0 1.924999230000308E-4 1.049999580000168E-4 28-29 0.0 0.0 0.0 4.549998180000728E-4 1.049999580000168E-4 30-31 0.0 0.0 0.0 0.0010149995940001624 1.049999580000168E-4 32-33 0.0 0.0 0.0 0.002432499027000389 1.74999930000028E-4 34-35 0.0 0.0 0.0 0.0037099985160005938 1.74999930000028E-4 36-37 0.0 0.0 0.0 0.00568749772500091 1.74999930000028E-4 38-39 0.0 0.0 0.0 0.007822496871001252 1.74999930000028E-4 40-41 0.0 0.0 0.0 0.009607496157001537 1.74999930000028E-4 42-43 0.0 0.0 0.0 0.012512494995002002 1.74999930000028E-4 44-45 0.0 0.0 0.0 0.015854993658002536 1.74999930000028E-4 46-47 0.0 0.0 0.0 0.020982491607003356 1.924999230000308E-4 48-49 0.0 0.0 0.0 0.027649988940004422 2.099999160000336E-4 50-51 0.0 0.0 0.0 0.03456248617500553 2.099999160000336E-4 52-53 0.0 0.0 0.0 0.04521998191200723 2.2749990900003641E-4 54-55 0.0 0.0 0.0 0.0649774740090104 2.449999020000392E-4 56-57 0.0 0.0 0.0 0.09691496123401551 2.449999020000392E-4 58-59 0.0 0.0 0.0 0.13355994657602135 2.449999020000392E-4 60-61 0.0 0.0 0.0 0.17043243182702728 2.449999020000392E-4 62-63 0.0 0.0 0.0 0.2148824140470344 2.449999020000392E-4 64-65 0.0 0.0 0.0 0.2674873930050428 2.449999020000392E-4 66-67 0.0 0.0 0.0 0.343717362513055 2.449999020000392E-4 68-69 0.0 0.0 0.0 0.4400373239850704 2.449999020000392E-4 70-71 0.0 0.0 0.0 0.5326647869340853 2.799998880000448E-4 72-73 0.0 0.0 0.0 0.6242247503100999 3.849998460000616E-4 74-75 0.0 0.0 0.0 0.7256897097241162 3.849998460000616E-4 76-77 0.0 0.0 0.0 0.843499662600135 4.0249983900006437E-4 78-79 0.0 0.0 0.0 0.9549571180171528 4.199998320000672E-4 80-81 0.0 0.0 0.0 1.0797145681141727 4.199998320000672E-4 82-83 0.0 0.0 0.0 1.2216045113581955 4.549998180000728E-4 84-85 0.0 0.0 0.0 1.392876942849223 4.549998180000728E-4 86-87 0.0 0.0 0.0 1.606219357512257 4.549998180000728E-4 88-89 0.0 0.0 0.0 1.8588142564742975 4.549998180000728E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13170 0.0 28.100847 1 GTATCAA 18095 0.0 26.307335 1 GTACATG 12935 0.0 24.387617 1 TACATGG 13390 0.0 22.915852 2 GAGTACT 11485 0.0 22.846357 12-13 ACATGGG 13155 0.0 22.130972 3 ATCAACG 21640 0.0 21.924974 3 TCAACGC 21775 0.0 21.766092 4 GTACTTT 12035 0.0 21.723356 14-15 TATCAAC 22055 0.0 21.579668 2 CAACGCA 22150 0.0 21.39759 5 AACGCAG 22570 0.0 21.020082 6 AGTACTT 12095 0.0 20.025343 12-13 ACGCAGA 24940 0.0 19.041626 7 CGCAGAG 25210 0.0 18.818851 8 TAAGGTG 1030 0.0 18.442968 5 ACTTTTT 14445 0.0 18.345778 16-17 AGAGTAC 20400 0.0 18.250986 10-11 TACACCG 235 2.5348527E-6 18.187908 5 GCAGAGT 26245 0.0 17.805288 9 >>END_MODULE