FastQCFastQC Report
Thu 26 May 2016
SRR936403_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936403_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences852584
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39170.4594268717217307No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29880.3504640012010547No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16960.19892468073527064No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15410.18074465389920524No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15060.17663948654912595No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT11800.1384027849455303No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC9830.11529655728936973No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58800.026.4257221
GTACATG34000.025.296251
CGTTGCG500.001658276223.73939930-31
TACATGG37150.022.8955292
ATCAACG68100.022.6773723
TCAACGC68250.022.5579074
GAGTACT32700.022.4484412-13
TATCAAC69100.022.3491882
CAACGCA69400.022.2525795
ACATGGG36400.022.061843
AACGCAG70100.022.030376
CATGGGG16250.021.638884
TGCACCG2205.6126737E-821.5990095
AGTACTT34050.021.4886512-13
GTGTAGC5350.021.3164021
GTACTTT34350.021.16389714-15
GCACCGT2056.800419E-720.861486
GGTATCA42800.020.2061731
ACGCAGA76600.020.097747
CGCAGAG77100.020.0290328
Produced by FastQC (version 0.11.5)