##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936403_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 852584 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.05673458568305 33.0 31.0 34.0 31.0 34.0 2 32.16307132200463 33.0 31.0 34.0 31.0 34.0 3 32.20827977067362 34.0 31.0 34.0 30.0 34.0 4 35.82692145290083 37.0 35.0 37.0 35.0 37.0 5 35.8424260835296 37.0 35.0 37.0 35.0 37.0 6 35.790306878852995 37.0 35.0 37.0 35.0 37.0 7 35.76658487609432 37.0 35.0 37.0 35.0 37.0 8 35.76226624004204 37.0 35.0 37.0 35.0 37.0 9 37.39532527000272 39.0 37.0 39.0 35.0 39.0 10-11 37.31500767079842 39.0 37.0 39.0 34.5 39.0 12-13 37.24666953637413 39.0 37.0 39.0 34.0 39.0 14-15 38.49731991217288 40.0 38.0 41.0 34.0 41.0 16-17 38.43103260206619 40.0 38.0 41.0 34.0 41.0 18-19 38.41573733497228 40.0 38.0 41.0 34.0 41.0 20-21 38.31073536449195 40.0 38.0 41.0 34.0 41.0 22-23 38.386840475542584 40.0 38.0 41.0 34.0 41.0 24-25 38.41971993375432 40.0 38.0 41.0 34.0 41.0 26-27 38.34198565771818 40.0 38.0 41.0 34.0 41.0 28-29 38.214523730213095 40.0 38.0 41.0 34.0 41.0 30-31 38.023558968969624 40.0 38.0 41.0 34.0 41.0 32-33 37.825050083041674 40.0 37.0 41.0 33.0 41.0 34-35 37.62760795417226 40.0 37.0 41.0 33.0 41.0 36-37 37.4603176930367 40.0 37.0 41.0 33.0 41.0 38-39 37.26008815553658 40.0 37.0 41.0 32.5 41.0 40-41 37.07221634466516 39.0 36.0 41.0 31.5 41.0 42-43 36.87519528867537 39.0 36.0 41.0 31.0 41.0 44-45 36.614042135437685 39.0 36.0 40.5 31.0 41.0 46-47 36.45343684610549 39.0 36.0 41.0 30.5 41.0 48-49 36.316845026413816 39.0 36.0 41.0 30.0 41.0 50-51 35.337553249885055 38.0 35.0 40.0 29.0 40.5 52-53 35.459249176620716 38.5 35.0 39.5 29.0 40.5 54-55 35.90254450001407 39.0 35.0 40.5 29.0 41.0 56-57 35.80413366894054 39.0 35.0 41.0 29.0 41.0 58-59 35.59607440439886 39.0 35.0 40.5 28.0 41.0 60-61 35.33552471076164 38.5 35.0 40.0 28.0 41.0 62-63 35.00666796468148 38.0 35.0 40.0 27.5 41.0 64-65 34.65456306944536 37.5 34.0 40.0 26.5 41.0 66-67 34.16786439811209 37.0 34.0 40.0 26.0 41.0 68-69 33.819749725540234 36.5 34.0 39.0 26.0 41.0 70-71 33.421205417882575 36.0 33.5 39.0 25.0 40.5 72-73 33.03425468927402 35.5 33.0 38.5 24.5 40.0 74-75 32.60732842746286 35.0 33.0 37.5 23.5 39.5 76-77 32.12265653589559 35.0 32.0 37.0 21.5 39.0 78-79 31.66284612425286 35.0 32.0 36.5 20.0 39.0 80-81 31.230103426759122 35.0 31.0 36.0 18.5 38.5 82-83 30.816545349197263 34.5 31.0 35.5 17.5 37.0 84-85 30.47652899890216 34.0 31.0 35.0 14.0 37.0 86-87 30.16423484372214 34.0 31.0 35.0 8.5 36.0 88-89 29.77070294539893 34.0 30.0 35.0 4.5 36.0 90-91 29.39118726131384 34.0 30.0 35.0 2.0 36.0 92-93 28.966274290861662 34.0 29.5 35.0 2.0 35.0 94-95 28.4678066911882 34.0 29.0 35.0 2.0 35.0 96-97 27.949585026226156 34.0 29.0 35.0 2.0 35.0 98-99 27.309151356347293 34.0 27.5 35.0 2.0 35.0 100-101 24.63491163334053 31.0 21.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3520913827525689 1101 2 0.36658628926650394 1101 3 0.35391810254139955 1101 4 0.43058732104809394 1101 5 0.402106248272311 1101 6 0.3464867691416842 1101 7 0.3523816487071372 1101 8 0.4495125658160788 1101 9 0.5208889477620886 1101 10-11 0.623551008289688 1101 12-13 0.5314358589993233 1101 14-15 0.6008447894911697 1101 16-17 0.6043931892777223 1101 18-19 0.5541007086218883 1101 20-21 0.6365430200320006 1101 22-23 0.562873929885356 1101 24-25 0.5489765529117747 1101 26-27 0.5585094653683953 1101 28-29 0.5617829471639411 1101 30-31 0.6270838003828771 1101 32-33 0.5865447769778456 1101 34-35 0.5751283580841218 1101 36-37 0.45414431510345565 1101 38-39 0.4591010816091341 1101 40-41 0.4576790202202332 1101 42-43 0.5320346464687944 1101 44-45 0.7026478965234801 1101 46-47 0.7623023039448711 1101 48-49 0.9301529587091366 1101 50-51 0.7631072903763751 1101 52-53 0.8397004516016437 1101 54-55 0.7735969197086447 1101 56-57 0.7913063319818647 1101 58-59 0.8041406642752946 1101 60-61 0.742648434133578 1101 62-63 0.699152892985289 1101 64-65 0.6429739407549064 1101 66-67 0.7313753971423722 1101 68-69 0.57478379137563 1101 70-71 0.6342086667373863 1101 72-73 0.6917648063879582 1101 74-75 0.8145221579028927 1101 76-77 0.8123613014877122 1101 78-79 0.8835241977548911 1101 80-81 0.7402154735890747 1101 82-83 0.692988775624034 1101 84-85 0.6849121802385731 1101 86-87 0.7807297435046827 1101 88-89 0.7953613731891558 1101 90-91 0.8085456457697688 1101 92-93 0.8087771155107859 1101 94-95 0.7192783781749945 1101 96-97 0.6924270455717512 1101 98-99 0.7293432527231296 1101 100-101 0.9462371616339702 1102 1 0.3199034682630426 1102 2 0.3481684854605369 1102 3 0.4051171608047923 1102 4 0.3167237580899567 1102 5 0.3126609449049198 1102 6 0.33124240783879344 1102 7 0.44589852012172315 1102 8 0.4505026680725379 1102 9 0.4785070296295615 1102 10-11 0.5284471696965198 1102 12-13 0.5546255490820755 1102 14-15 0.5244547688824852 1102 16-17 0.48198997386367637 1102 18-19 0.4765865555208322 1102 20-21 0.33499982786624827 1102 22-23 0.3554883310448105 1102 24-25 0.3647776930295237 1102 26-27 0.20364912733348461 1102 28-29 0.14258137388669212 1102 30-31 0.4326607698075975 1102 32-33 0.31121683327218364 1102 34-35 0.317081953291499 1102 36-37 0.18596481459766778 1102 38-39 0.1753962071712749 1102 40-41 0.3423450594996993 1102 42-43 0.4076345035032105 1102 44-45 0.4750517677429684 1102 46-47 0.6710579623161479 1102 48-49 0.8214908345881113 1102 50-51 0.8276114112937876 1102 52-53 0.9465846871866361 1102 54-55 0.8813533765230446 1102 56-57 0.877805511405306 1102 58-59 0.8585190672906862 1102 60-61 0.9441996599159097 1102 62-63 0.9721408409208934 1102 64-65 0.9495488001256192 1102 66-67 1.0000770827262926 1102 68-69 0.8543613543042596 1102 70-71 0.782104760924156 1102 72-73 0.7268768753393431 1102 74-75 0.8599105340794537 1102 76-77 0.919193072637853 1102 78-79 0.8764674853403243 1102 80-81 0.9985408416188832 1102 82-83 1.1427078449705412 1102 84-85 0.772914547696196 1102 86-87 0.8533040421617599 1102 88-89 0.939700050790286 1102 90-91 1.0298082515798797 1102 92-93 1.0202786312410979 1102 94-95 0.8338310286946395 1102 96-97 0.748330196225016 1102 98-99 0.8445071139087936 1102 100-101 0.9545214598483795 1103 1 0.287262667238366 1103 2 0.07868621932117392 1103 3 0.20099742625367867 1103 4 0.21951475124041764 1103 5 0.284733704770602 1103 6 0.2393176788591873 1103 7 0.3763926768218937 1103 8 0.32131807294010173 1103 9 0.16667662800385585 1103 10-11 0.3233593213837409 1103 12-13 0.13244772115604775 1103 14-15 0.29293617520397675 1103 16-17 0.43101442206923934 1103 18-19 0.41445067997081253 1103 20-21 0.3806080671482306 1103 22-23 0.14654557021546566 1103 24-25 0.28273455431067873 1103 26-27 0.16707552495855538 1103 28-29 0.21950466671783175 1103 30-31 0.2753440377891536 1103 32-33 0.319223010113987 1103 34-35 0.4377883605719788 1103 36-37 0.6224374655593223 1103 38-39 0.5451197649647952 1103 40-41 0.40442105654693705 1103 42-43 0.39924660120620814 1103 44-45 0.6001026410802908 1103 46-47 0.6493274455178906 1103 48-49 0.7766067509490782 1103 50-51 0.7439101526289491 1103 52-53 0.9114058329056505 1103 54-55 0.9504945690969819 1103 56-57 0.7324731342568143 1103 58-59 0.6763586041304634 1103 60-61 0.7911813155752228 1103 62-63 0.7808809504424161 1103 64-65 0.6937167275154579 1103 66-67 0.8848062868755022 1103 68-69 0.6570277721008964 1103 70-71 0.693960330233061 1103 72-73 0.6214266217445257 1103 74-75 0.6310736085037547 1103 76-77 0.7327997139122857 1103 78-79 0.652328683228486 1103 80-81 0.7115186182739421 1103 82-83 0.6671644709478919 1103 84-85 0.6480610862977159 1103 86-87 0.6812141607866877 1103 88-89 0.7352058416964233 1103 90-91 0.7625407883420046 1103 92-93 0.4377878071538497 1103 94-95 0.23763668178666109 1103 96-97 0.5073117377409346 1103 98-99 0.6044736100152868 1103 100-101 0.529341286369128 1104 1 0.4591929676142854 1104 2 0.48705183570447375 1104 3 0.34298420516884676 1104 4 0.3610495063717991 1104 5 0.4129881233030872 1104 6 0.45577843036705445 1104 7 0.4859589661097843 1104 8 0.516535539139106 1104 9 0.59560566149149 1104 10-11 0.7546123068786628 1104 12-13 0.5588203768935145 1104 14-15 0.4836080535826994 1104 16-17 0.5028602428650188 1104 18-19 0.5640448969984249 1104 20-21 0.49168252768227916 1104 22-23 0.5000138143229691 1104 24-25 0.50603949566365 1104 26-27 0.4101479550506397 1104 28-29 0.5540538829452473 1104 30-31 0.6059472085527489 1104 32-33 0.5208243959944738 1104 34-35 0.6128235756681022 1104 36-37 0.6237535266973779 1104 38-39 0.5568873489954029 1104 40-41 0.3908823333797429 1104 42-43 0.5645743001241641 1104 44-45 0.6521079774043059 1104 46-47 0.8420612242779058 1104 48-49 0.9808628602948559 1104 50-51 0.8794204691555407 1104 52-53 0.9871247034325918 1104 54-55 0.9834758125533298 1104 56-57 0.8986929693047827 1104 58-59 1.0410101609858913 1104 60-61 0.9573739681118667 1104 62-63 1.0319758864321855 1104 64-65 1.069928792641086 1104 66-67 1.0690188064682857 1104 68-69 0.9549380172810444 1104 70-71 0.9296549991622953 1104 72-73 0.8886386489978975 1104 74-75 1.0142987615504566 1104 76-77 0.9025117381114427 1104 78-79 0.8361899681244189 1104 80-81 1.069861999973817 1104 82-83 0.9412087766088213 1104 84-85 0.9461069386129957 1104 86-87 1.038523996213378 1104 88-89 1.21946655679805 1104 90-91 1.1627476808495274 1104 92-93 0.9703200810411658 1104 94-95 1.0774996208453018 1104 96-97 1.1481061512156394 1104 98-99 1.3803990065439855 1104 100-101 1.246459915735283 1105 1 0.4852364393494639 1105 2 0.3636695182125358 1105 3 0.39999276034083664 1105 4 0.40165951151126933 1105 5 0.3104352163184103 1105 6 0.456716724881467 1105 7 0.4799239540343052 1105 8 0.614693516461756 1105 9 0.7115707676693148 1105 10-11 0.585412063094914 1105 12-13 0.347455324384498 1105 14-15 0.5245714541839703 1105 16-17 0.5777034435272341 1105 18-19 0.547069179860344 1105 20-21 0.5376719212702241 1105 22-23 0.35705557138518884 1105 24-25 0.2851955309031311 1105 26-27 0.32105909678632116 1105 28-29 0.12162382295443308 1105 30-31 0.1763136118783919 1105 32-33 0.1364654426272125 1105 34-35 0.232862508295554 1105 36-37 0.31263384980179865 1105 38-39 0.24822734089960363 1105 40-41 0.21032818018712618 1105 42-43 0.18453272953831146 1105 44-45 0.4059298333444943 1105 46-47 0.33380191296697603 1105 48-49 0.32398006296737236 1105 50-51 0.2608874469193765 1105 52-53 0.4879774917091453 1105 54-55 0.32482944906994504 1105 56-57 0.2780366179651068 1105 58-59 0.36795281267987434 1105 60-61 0.47374977712367894 1105 62-63 0.39888110640607977 1105 64-65 0.48940693169787863 1105 66-67 0.4144380826670826 1105 68-69 0.739048411291968 1105 70-71 0.6357611906325857 1105 72-73 0.3611759101603269 1105 74-75 0.6415277226951517 1105 76-77 0.6983232969174509 1105 78-79 0.8754366490724443 1105 80-81 1.0255352024098379 1105 82-83 0.5896362350268625 1105 84-85 0.6341328815694176 1105 86-87 0.5610980978293547 1105 88-89 0.6936577723965733 1105 90-91 0.6772871524391704 1105 92-93 0.5051140701345744 1105 94-95 0.7057642080730346 1105 96-97 0.9126015052251084 1105 98-99 1.023847545888227 1105 100-101 1.112480964824048 1106 1 0.22208843448597548 1106 2 0.23640817927881486 1106 3 0.19161429234039673 1106 4 0.29017872598384287 1106 5 0.14945367547614552 1106 6 0.29943070004819106 1106 7 0.4839142270529848 1106 8 0.3889170159643527 1106 9 0.3807023398295186 1106 10-11 0.36212994379202 1106 12-13 0.44320898121365104 1106 14-15 0.4559999203421796 1106 16-17 0.38128658342628086 1106 18-19 0.2469789105261242 1106 20-21 0.12894342932077052 1106 22-23 0.3745880867678224 1106 24-25 0.36207552795556097 1106 26-27 0.3567873308233871 1106 28-29 0.38151292062953956 1106 30-31 0.45586668656244456 1106 32-33 0.4869610028497533 1106 34-35 0.3665384966526517 1106 36-37 0.4206199225330849 1106 38-39 0.4135841811044543 1106 40-41 0.46973829772148434 1106 42-43 0.4440349586381984 1106 44-45 0.45884792774042893 1106 46-47 0.6866610559609683 1106 48-49 0.7865238159609973 1106 50-51 0.35228975339297364 1106 52-53 0.4028332449975309 1106 54-55 0.36520563270357087 1106 56-57 0.2164784587226407 1106 58-59 0.4893134847258551 1106 60-61 0.6157342286914158 1106 62-63 0.5372124132849692 1106 64-65 0.49841175834391294 1106 66-67 0.5109525810274889 1106 68-69 0.6839841174192287 1106 70-71 0.3890698380663622 1106 72-73 0.26676523995657675 1106 74-75 0.4465091161589143 1106 76-77 0.4257264876620752 1106 78-79 0.5503067717650474 1106 80-81 0.6720220673005954 1106 82-83 0.46361103330253073 1106 84-85 0.43797577608325255 1106 86-87 0.4969460769016081 1106 88-89 0.510166007170529 1106 90-91 0.3096920011919764 1106 92-93 0.27283080864826204 1106 94-95 0.2504935842935154 1106 96-97 0.06109824447569068 1106 98-99 0.28528227563289477 1106 100-101 0.16955881751923485 1107 1 0.14642030524784388 1107 2 0.1406673646708292 1107 3 0.15702669317361995 1107 4 0.2825511941839949 1107 5 0.247516876291634 1107 6 0.1527879663691536 1107 7 0.306592948854167 1107 8 0.30009017556192674 1107 9 0.39394082761926086 1107 10-11 0.4060336799973143 1107 12-13 0.3587410469850454 1107 14-15 0.20876329400702787 1107 16-17 0.2334627124871318 1107 18-19 0.3171756928689007 1107 20-21 0.3900611884840046 1107 22-23 0.39414887212437577 1107 24-25 0.4957944483118979 1107 26-27 0.28955336370908213 1107 28-29 0.3034758688190564 1107 30-31 0.2270207884106128 1107 32-33 0.06787222919376745 1107 34-35 0.25847219341283534 1107 36-37 0.12875049027673668 1107 38-39 0.06968709295128406 1107 40-41 0.37036543063820915 1107 42-43 0.18685506234781002 1107 44-45 0.565617860816694 1107 46-47 0.6399540577781053 1107 48-49 0.5543915640441455 1107 50-51 0.7404229803574722 1107 52-53 0.8418218546682326 1107 54-55 0.839444430735746 1107 56-57 0.5698822614084449 1107 58-59 0.6876315275393097 1107 60-61 0.7569521518399327 1107 62-63 0.6926416558502311 1107 64-65 0.7778825733951038 1107 66-67 0.7221966531810224 1107 68-69 0.8083691725980628 1107 70-71 0.7548345856619818 1107 72-73 0.6094501666064005 1107 74-75 0.6435706771215521 1107 76-77 0.5754782937162375 1107 78-79 0.7402349728021456 1107 80-81 0.7918642868635928 1107 82-83 0.7702834616143726 1107 84-85 0.5728558895433409 1107 86-87 0.71523930032048 1107 88-89 0.4163863679381592 1107 90-91 0.3580100440054359 1107 92-93 0.4948934776195877 1107 94-95 0.4031683172634217 1107 96-97 0.5798036471260453 1107 98-99 0.5853398920846082 1107 100-101 0.61132852899377 1108 1 0.23981541097366943 1108 2 0.1660692811989044 1108 3 0.2229172451653838 1108 4 0.26714769374842007 1108 5 0.05398317865957836 1108 6 0.1914194702893255 1108 7 0.3665557270279223 1108 8 0.41402856156018686 1108 9 0.32512004675286477 1108 10-11 0.26418288408378743 1108 12-13 0.07871808032630412 1108 14-15 0.37406780022386954 1108 16-17 0.4588032525995942 1108 18-19 0.6061695156296452 1108 20-21 0.4960562783707516 1108 22-23 0.5259755134485289 1108 24-25 0.44929290400208544 1108 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-1.1014584164873114 2316 66-67 -1.0840880975253882 2316 68-69 -1.1499983417579713 2316 70-71 -0.9018193200895439 2316 72-73 -0.4831712899609144 2316 74-75 -0.45142522115572703 2316 76-77 -0.6215183337561925 2316 78-79 -1.4419498781685967 2316 80-81 -1.3373787174204281 2316 82-83 -1.2503595973592851 2316 84-85 -1.2783244951070998 2316 86-87 -0.9761584756343105 2316 88-89 -1.2592197755309904 2316 90-91 -1.2182478176297877 2316 92-93 -1.8387178613650086 2316 94-95 -1.3627759904569317 2316 96-97 -1.6873871285552973 2316 98-99 -2.845618999538761 2316 100-101 -4.1876118593026455 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1573.0 3 506.0 4 446.0 5 473.0 6 498.0 7 546.0 8 700.0 9 803.0 10 1022.0 11 1460.0 12 2066.0 13 2656.0 14 3281.0 15 4309.0 16 5766.0 17 8412.0 18 6436.0 19 3926.0 20 3744.0 21 3831.0 22 4132.0 23 4592.0 24 5150.0 25 5907.0 26 6860.0 27 8166.0 28 10206.0 29 12740.0 30 16120.0 31 21115.0 32 28128.0 33 38871.0 34 56411.0 35 85060.0 36 129222.0 37 184621.0 38 157644.0 39 25166.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.648998667392405 18.69709454006269 14.617508774564087 23.03639801798082 2 15.158336539634576 19.827375869220447 40.91001867474968 24.104268916395306 3 19.008946973900457 24.611627194953 30.416661290114455 25.962764541032087 4 12.25502790379555 16.376483617298593 38.22732002726442 33.14116845164144 5 13.20118885443104 37.073906804232095 35.060058738701954 14.66484560263491 6 31.27767365825665 36.15199285545798 17.98539875299107 14.584934733294293 7 27.657882327548794 32.975993018198196 21.373439593103594 17.992685061149416 8 25.843288537846203 34.0417543686746 20.390394195593665 19.72456289788553 9 25.995477741311312 16.747141906694093 21.162973801642515 36.094406550352076 10-11 24.452888376202978 26.123824600396407 28.02335719826276 21.399929825137857 12-13 25.76523637933806 23.905988010602485 28.22019987022633 22.108575739833125 14-15 22.63637462264007 25.162178239428933 27.08045143569834 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21.661194994446223 26.52019240581158 29.977081141438916 21.841531458303276 42-43 21.558808212365467 26.708016252076085 29.33842227247563 22.39475326308283 44-45 21.64445549594522 26.62232251980473 29.331236936617817 22.401985047632227 46-47 21.9449329945184 26.748883828428905 29.01500681168149 22.291176365371207 48-49 21.946872553360564 26.85220318506637 28.73990188517692 22.46102237639614 50-51 21.702636764567888 27.090703293609835 28.785246786149948 22.42141315567233 52-53 22.030533599040076 27.17158376624062 28.457462093977142 22.340420540742155 54-55 21.53060548248405 26.834566592164816 28.86834402379211 22.766483901559027 56-57 21.859715546422514 27.185009770321212 28.428878395274587 22.526396287981683 58-59 22.000819897589043 26.72449740219144 28.60210979941833 22.67257290080119 60-61 21.98723974995587 26.699582849257453 29.068891157846956 22.244286242939726 62-63 21.704963103234117 26.857960206736838 28.99611356802839 22.440963122000653 64-65 21.523279592625457 26.932146662811707 28.869556752052443 22.675016992510393 66-67 21.55983642902892 26.980177694568987 28.477877331156144 22.982108545245953 68-69 21.439977941262743 26.803829772255273 28.842972297511356 22.91321998897063 70-71 21.62845289523663 26.75053963176549 28.599808848024793 23.021198624973085 72-73 21.7021835679881 26.500367173514704 28.809513195958043 22.987936062539152 74-75 21.448520746158948 27.063426091863736 28.4500230835519 23.038030078425415 76-77 21.606964254812418 26.606362977758614 28.61527361039007 23.171399157038902 78-79 21.56204873818321 26.734473659658885 28.645168634395564 23.05830896776234 80-81 21.57330658608059 26.98358013699555 28.374898978842634 23.06821429808123 82-83 21.657775660312705 26.770593260515486 28.504867561947588 23.066763517224224 84-85 21.458691256803355 26.749985482653383 28.30636235179842 23.48496090874484 86-87 21.522387919626855 26.79048220134581 28.45033692153201 23.236792957495332 88-89 21.610717784177893 26.657203357519517 28.10268472516321 23.629394133139375 90-91 21.499760941684436 26.710176257842726 28.43831855473849 23.35174424573435 92-93 21.719541152234406 26.82538397646164 28.355130713893544 23.099944157410405 94-95 21.641258803601676 26.58728762264045 28.193226446699793 23.578227127058085 96-97 21.622528443285884 26.55608061023137 28.179852604065236 23.64153834241751 98-99 21.822301193210425 26.785796019345803 27.525699149484023 23.86620363795975 100-101 21.984610508522326 26.520108465981124 27.582112504108558 23.91316852138799 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 105.0 1 91.5 2 120.0 3 148.5 4 119.0 5 125.0 6 231.5 7 472.5 8 820.5 9 1034.0 10 943.0 11 801.5 12 795.5 13 874.0 14 941.5 15 996.5 16 1079.0 17 1136.5 18 1179.0 19 1265.5 20 1458.0 21 1675.0 22 1973.0 23 2411.5 24 2864.5 25 3461.5 26 4650.0 27 6023.5 28 7058.5 29 8360.5 30 10121.5 31 12192.0 32 14489.0 33 16454.0 34 18602.5 35 20540.5 36 22233.5 37 24135.5 38 25281.5 39 25298.0 40 25690.5 41 27093.0 42 28007.5 43 28715.0 44 29578.5 45 30766.5 46 31212.5 47 32323.0 48 38172.0 49 38207.5 50 32149.0 51 29339.5 52 27484.0 53 26939.5 54 26272.0 55 24105.0 56 21687.0 57 19264.5 58 17245.5 59 15134.0 60 12701.5 61 10512.5 62 8689.5 63 6892.0 64 5128.0 65 3943.0 66 3017.5 67 2407.0 68 1777.0 69 1211.0 70 935.0 71 773.5 72 668.5 73 550.5 74 447.5 75 353.0 76 233.5 77 128.0 78 85.0 79 65.0 80 44.0 81 33.0 82 20.0 83 11.0 84 10.5 85 10.0 86 8.0 87 6.5 88 5.5 89 5.0 90 4.5 91 3.5 92 2.5 93 1.5 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014074859485986132 2 0.011259887588788906 3 0.013722987998836478 4 0.02240248468186126 5 0.039292316065044616 6 0.05454008050819626 7 0.00961782064875719 8 0.03119927186060259 9 0.04163812597937564 10-11 0.05008304167096731 12-13 0.03882315408217841 14-15 0.03295862929635086 16-17 0.03178572433918535 18-19 0.02515881133120021 20-21 0.052252915841723514 22-23 0.010732080358064425 24-25 0.0031668433843468798 26-27 9.969692135906843E-4 28-29 0.0 30-31 0.0018766479314648176 32-33 0.0013488407007403377 34-35 0.0 36-37 1.172904957165511E-4 38-39 0.0 40-41 0.0012901954528820622 42-43 0.0018766479314648176 44-45 0.001172904957165511 46-47 5.278072307244799E-4 48-49 0.0013488407007403377 50-51 0.0028149718971972265 52-53 0.0017593574357482665 54-55 0.003811941110787911 56-57 2.345809914331022E-4 58-59 0.0045156840850872175 60-61 0.0017007121878899907 62-63 2.9322623929137773E-4 64-65 2.9322623929137773E-4 66-67 0.012901954528820621 68-69 0.03682921565499705 70-71 0.044863614611580796 72-73 0.014778602460285438 74-75 0.028794816698413297 76-77 0.027152749758381577 78-79 0.05389498278175523 80-81 0.0772357914293489 82-83 0.04832368423521905 84-85 0.018238672083923695 86-87 0.018766479314648175 88-89 0.019822093776097133 90-91 0.033017274544209135 92-93 0.022050613194711605 94-95 0.010790725605922702 96-97 0.021816032203278504 98-99 0.06017002430259072 100-101 0.08257250898445198 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 852584.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.71434015718168 #Duplication Level Percentage of deduplicated Percentage of total 1 80.45453680543957 48.84744114793951 2 11.862597573369388 14.40459568434614 3 3.454040634667048 6.291293940297085 4 1.4005400532643055 3.401314607905856 5 0.7087083618485412 2.1514380276755665 6 0.4312961073890074 1.5711515143490733 7 0.27693517195340267 1.1769755362026542 8 0.19744913050472201 0.9590394938562761 9 0.1527978593971945 0.8349319089657439 >10 0.945745700601858 10.980198466365167 >50 0.07393563695117518 3.064206734501742 >100 0.03870744358732351 4.149096055701678 >500 0.0015482977294031747 0.6609647461470215 >1k 0.0011612232970523812 1.5073521357464874 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3917 0.4594268717217307 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2988 0.3504640012010547 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1696 0.19892468073527064 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1541 0.18074465389920524 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1506 0.17663948654912595 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1180 0.1384027849455303 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 983 0.11529655728936973 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 1.172904957165511E-4 4 0.0 0.0 0.0 0.0 1.172904957165511E-4 5 0.0 0.0 0.0 0.0 1.172904957165511E-4 6 0.0 0.0 0.0 0.0 1.172904957165511E-4 7 0.0 0.0 0.0 0.0 1.172904957165511E-4 8 0.0 0.0 0.0 0.0 1.172904957165511E-4 9 0.0 0.0 0.0 0.0 1.172904957165511E-4 10-11 0.0 0.0 0.0 0.0 1.172904957165511E-4 12-13 0.0 0.0 0.0 1.172904957165511E-4 1.172904957165511E-4 14-15 0.0 0.0 0.0 1.172904957165511E-4 1.172904957165511E-4 16-17 0.0 0.0 0.0 2.345809914331022E-4 1.7593574357482666E-4 18-19 0.0 0.0 0.0 3.5187148714965326E-4 2.345809914331022E-4 20-21 0.0 0.0 0.0 4.1051673500792885E-4 2.345809914331022E-4 22-23 0.0 0.0 0.0 5.864524785827555E-4 2.345809914331022E-4 24-25 0.0 0.0 0.0 0.0013488407007403375 2.345809914331022E-4 26-27 0.0 0.0 0.0 0.001700712187889991 2.345809914331022E-4 28-29 0.0 0.0 0.0 0.004222457845795839 2.345809914331022E-4 30-31 0.0 0.0 0.0 0.011435823332363731 2.345809914331022E-4 32-33 0.0 0.0 0.0 0.022578420425436084 2.345809914331022E-4 34-35 0.0 0.0 0.0 0.04069980201364323 2.345809914331022E-4 36-37 0.0 0.0 0.0 0.0657413228491269 2.345809914331022E-4 38-39 0.0 0.0 0.0 0.109607968247117 2.345809914331022E-4 40-41 0.0 0.0 0.0 0.16649385866964428 2.345809914331022E-4 42-43 0.0 0.0 0.0 0.22801272367297531 2.345809914331022E-4 44-45 0.0 0.0 0.0 0.30307864093156806 2.345809914331022E-4 46-47 0.0 0.0 0.0 0.4164985502894729 2.345809914331022E-4 48-49 0.0 0.0 0.0 0.5298011691516613 2.345809914331022E-4 50-51 0.0 0.0 0.0 0.6646265939778369 2.345809914331022E-4 52-53 0.0 0.0 0.0 0.7851425783265931 2.345809914331022E-4 54-55 0.0 0.0 0.0 0.9199093579049102 2.345809914331022E-4 56-57 0.0 0.0 0.0 1.1000675593255327 2.345809914331022E-4 58-59 0.0 0.0 0.0 1.2954148799414487 2.345809914331022E-4 60-61 0.0 0.0 0.0 1.5073588057012564 2.345809914331022E-4 62-63 0.0 0.0 0.0 1.740942827920768 2.345809914331022E-4 64-65 0.0 0.0 0.0 1.9875460951648167 2.345809914331022E-4 66-67 0.0 0.0 0.0 2.273910840456776 2.345809914331022E-4 68-69 0.0 0.0 0.0 2.62677929682002 2.345809914331022E-4 70-71 0.0 0.0 0.0 3.0383516462893976 2.345809914331022E-4 72-73 0.0 0.0 0.0 3.4357904910249313 2.345809914331022E-4 74-75 0.0 0.0 0.0 3.8416742514520563 2.345809914331022E-4 76-77 0.0 0.0 0.0 4.339982922503824 2.345809914331022E-4 78-79 0.0 0.0 0.0 4.871778030082666 2.345809914331022E-4 80-81 0.0 0.0 0.0 5.425623750856221 2.345809914331022E-4 82-83 0.0 0.0 0.0 5.9856858679027525 2.345809914331022E-4 84-85 0.0 0.0 0.0 6.582753136347855 2.345809914331022E-4 86-87 0.0 0.0 0.0 7.191256228125322 2.345809914331022E-4 88-89 0.0 0.0 0.0 7.821282125866777 2.345809914331022E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5880 0.0 26.425722 1 GTACATG 3400 0.0 25.29625 1 CGTTGCG 50 0.0016582762 23.739399 30-31 TACATGG 3715 0.0 22.895529 2 ATCAACG 6810 0.0 22.677372 3 TCAACGC 6825 0.0 22.557907 4 GAGTACT 3270 0.0 22.44844 12-13 TATCAAC 6910 0.0 22.349188 2 CAACGCA 6940 0.0 22.252579 5 ACATGGG 3640 0.0 22.06184 3 AACGCAG 7010 0.0 22.03037 6 CATGGGG 1625 0.0 21.63888 4 TGCACCG 220 5.6126737E-8 21.599009 5 AGTACTT 3405 0.0 21.48865 12-13 GTGTAGC 535 0.0 21.316402 1 GTACTTT 3435 0.0 21.163897 14-15 GCACCGT 205 6.800419E-7 20.86148 6 GGTATCA 4280 0.0 20.206173 1 ACGCAGA 7660 0.0 20.09774 7 CGCAGAG 7710 0.0 20.029032 8 >>END_MODULE