FastQCFastQC Report
Thu 26 May 2016
SRR936403_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936403_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences852584
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32320.37908288215589314No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30910.3625449222598594No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27350.3207895057847672No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17670.20725230593114577No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10240.12010546761374832No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA8850.10380208870914773No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG608.2907E-539.5819134
GTAAGGT3450.027.5352464
TAAGGTG4000.026.1240635
CGTATAG950.001226131325.0005721
GTACATG35800.023.0871181
GGTAAGG3950.022.8486233
ACCGTGC1302.9413588E-421.9222938
GAGTACT37550.021.82012412-13
TACATGG37600.021.6028882
ACATGGG36400.021.4015883
GGTGTGC9400.021.2226458
GTACTTT40150.020.82117314-15
AGTACTT39350.020.39952312-13
AGGTAAG4200.020.3576092
ATACGTC2351.1314296E-720.2120425
CATGGGG15100.019.5026134
ACTTTTT46700.017.9008616-17
GTAAGCG800.001191621617.81186394-95
TACTTTT44650.017.76532214-15
TAATACG2956.330811E-817.7122693
Produced by FastQC (version 0.11.5)