##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936403_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 852584 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.434358374072232 31.0 31.0 34.0 30.0 34.0 2 31.61048764696499 31.0 31.0 34.0 30.0 34.0 3 31.683173740065495 31.0 31.0 34.0 30.0 34.0 4 35.50484175166318 37.0 35.0 37.0 33.0 37.0 5 35.323149390558584 37.0 35.0 37.0 33.0 37.0 6 35.38439262289698 37.0 35.0 37.0 33.0 37.0 7 35.292727754684584 37.0 35.0 37.0 32.0 37.0 8 35.25374156681336 37.0 35.0 37.0 32.0 37.0 9 36.77822126617436 39.0 37.0 39.0 32.0 39.0 10-11 36.637280314901524 38.0 35.0 39.0 32.0 39.0 12-13 36.48953827423456 38.0 35.0 39.0 32.0 39.0 14-15 37.41996858960525 39.0 36.0 40.0 32.0 41.0 16-17 37.368786535989415 39.0 36.0 40.0 32.0 41.0 18-19 37.53385766094602 39.0 36.0 40.5 32.0 41.0 20-21 37.51723173317819 39.0 36.0 41.0 32.0 41.0 22-23 37.39608355305753 39.0 36.0 40.0 32.0 41.0 24-25 37.23931249003031 39.0 36.0 40.0 31.0 41.0 26-27 36.96402817786869 39.0 36.0 40.0 31.0 41.0 28-29 36.95076086344571 39.0 36.0 40.0 31.0 41.0 30-31 36.805264349319245 39.0 36.0 40.0 30.5 41.0 32-33 36.59154581835925 38.0 35.0 40.0 30.0 41.0 34-35 36.337550904075144 38.0 35.0 40.0 30.0 41.0 36-37 36.096645022660525 38.0 35.0 40.0 30.0 41.0 38-39 35.8494852120143 38.0 35.0 40.0 29.0 41.0 40-41 35.583844524410495 38.0 34.0 40.0 28.5 41.0 42-43 35.26823280755914 38.0 34.0 40.0 27.0 41.0 44-45 34.93582802398356 38.0 33.5 40.0 26.0 41.0 46-47 34.50364538860687 38.0 33.0 40.0 24.5 41.0 48-49 34.05428966529984 38.0 33.0 40.0 24.0 41.0 50-51 33.69001470822816 37.0 33.0 40.0 23.0 41.0 52-53 33.38133310031621 37.0 32.0 40.0 22.5 40.5 54-55 32.96898076905032 37.0 31.0 39.0 20.5 40.0 56-57 32.579812663620245 36.0 31.0 39.0 19.0 40.0 58-59 32.13459905416944 36.0 31.0 39.0 17.5 40.0 60-61 31.661532470700834 35.0 30.0 38.5 14.0 40.0 62-63 31.25324014994417 35.0 29.0 38.0 13.0 40.0 64-65 30.84950104623122 34.5 29.0 38.0 8.0 40.0 66-67 30.32278344421195 34.0 28.0 38.0 8.0 39.5 68-69 29.769000473853602 34.0 28.0 37.0 7.0 39.0 70-71 29.13698005123249 33.5 26.5 36.0 4.0 39.0 72-73 28.47356741388532 33.0 26.0 36.0 2.0 38.5 74-75 27.86999228228538 32.0 26.0 35.0 2.0 38.0 76-77 26.74761607067456 30.5 25.0 34.0 2.0 36.0 78-79 27.519715945877472 32.0 26.0 35.0 2.0 37.0 80-81 27.296067601550114 32.0 26.0 35.0 2.0 36.0 82-83 26.773043477240954 32.0 25.0 34.5 2.0 36.0 84-85 26.19532151670686 31.5 24.0 34.0 2.0 35.5 86-87 25.62111533878187 31.0 22.0 34.0 2.0 35.0 88-89 24.95454348193257 31.0 20.0 34.0 2.0 35.0 90-91 24.165612420594336 30.5 17.5 34.0 2.0 35.0 92-93 23.33381930695392 30.0 8.5 34.0 2.0 35.0 94-95 22.296227116624287 29.0 2.0 34.0 2.0 35.0 96-97 21.149468556763907 29.0 2.0 33.5 2.0 34.5 98-99 19.550960961031407 27.5 2.0 33.0 2.0 34.0 100-101 15.977649709588732 13.0 2.0 30.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4086804895725855 1101 2 0.5874866681696496 1101 3 0.5696378565399236 1101 4 0.37635831215313686 1101 5 0.5155176635545828 1101 6 0.5115123587904833 1101 7 0.4829956417263688 1101 8 0.494084875556112 1101 9 0.6621173300445307 1101 10-11 0.584760752952711 1101 12-13 0.5822097686928274 1101 14-15 0.6485348189487752 1101 16-17 0.6888161127881389 1101 18-19 0.7450199135556943 1101 20-21 0.6007460686191948 1101 22-23 0.7214709844107219 1101 24-25 0.6950041586122779 1101 26-27 0.5721042040051927 1101 28-29 0.8072804970910354 1101 30-31 0.9358352183584913 1101 32-33 0.8315735119035921 1101 34-35 0.9658406664098678 1101 36-37 1.079936012969931 1101 38-39 1.0319221681458046 1101 40-41 1.2198288522696217 1101 42-43 1.4389704932674547 1101 44-45 1.352928246588597 1101 46-47 1.3945844073112 1101 48-49 1.509851833600635 1101 50-51 1.5096837800234582 1101 52-53 1.3341253026364797 1101 54-55 1.4248470291772364 1101 56-57 1.461689235837781 1101 58-59 1.4284438948331086 1101 60-61 1.392485181011736 1101 62-63 1.3515301169522402 1101 64-65 1.4915691723126265 1101 66-67 1.4414161867209039 1101 68-69 1.2061630057426669 1101 70-71 1.1574948857843417 1101 72-73 1.3594717343849076 1101 74-75 1.4107216600916495 1101 76-77 1.4510313397508838 1101 78-79 1.5911268484704912 1101 80-81 1.5068566205271168 1101 82-83 1.4264834421298112 1101 84-85 1.298042417296859 1101 86-87 1.2760594993942895 1101 88-89 1.3078184111159246 1101 90-91 1.381454763526282 1101 92-93 1.387782427138184 1101 94-95 1.2794284582730668 1101 96-97 1.4436116541795343 1101 98-99 0.9460322980322182 1101 100-101 0.8252778147504625 1102 1 0.42896733887013383 1102 2 0.5389655914027074 1102 3 0.5550268393365094 1102 4 0.4589881575306123 1102 5 0.30819313659524283 1102 6 0.37879520931439004 1102 7 0.44505369034450837 1102 8 0.32543680206655523 1102 9 0.49060715768246865 1102 10-11 0.5680616512422105 1102 12-13 0.5773208211457828 1102 14-15 0.5932848505094128 1102 16-17 0.7309977643357612 1102 18-19 0.6789562873123245 1102 20-21 0.663399424621538 1102 22-23 0.4572336123542655 1102 24-25 0.5515152409043793 1102 26-27 0.6556329946835788 1102 28-29 0.7197256348675651 1102 30-31 0.7858917815773125 1102 32-33 0.9038640107583902 1102 34-35 0.8469675696802028 1102 36-37 0.9787037545563493 1102 38-39 1.045385115958453 1102 40-41 0.9940549608677216 1102 42-43 1.038016706236995 1102 44-45 1.0862544753630416 1102 46-47 1.0217018800993714 1102 48-49 1.2868891341743023 1102 50-51 1.2833367966464877 1102 52-53 1.305158212592751 1102 54-55 1.2888642145586786 1102 56-57 1.4246467216109053 1102 58-59 1.4304736126564208 1102 60-61 1.3436067722580418 1102 62-63 1.2535013861161062 1102 64-65 1.3274611578783464 1102 66-67 1.4880010799147918 1102 68-69 1.3002257532067496 1102 70-71 1.0552054690085129 1102 72-73 0.901564278368955 1102 74-75 1.0528300253818337 1102 76-77 1.231803780183256 1102 78-79 1.2903900510635502 1102 80-81 1.2007901743203782 1102 82-83 1.133629508034975 1102 84-85 0.9013343146567543 1102 86-87 1.1724219105941351 1102 88-89 1.1536505216453712 1102 90-91 1.3137781838139482 1102 92-93 1.4755187015402917 1102 94-95 1.4071609126085143 1102 96-97 1.479593567065617 1102 98-99 1.647634430711495 1102 100-101 0.9141694543788201 1103 1 0.2974741467752189 1103 2 0.41736311168421025 1103 3 0.41989836720199847 1103 4 0.31986981234788203 1103 5 0.3688739177477345 1103 6 0.35054144421962974 1103 7 0.297008770240204 1103 8 0.27953665508354675 1103 9 0.19924116998188168 1103 10-11 0.5075300985458497 1103 12-13 0.4125014998499026 1103 14-15 0.585006413600297 1103 16-17 0.6078913050358281 1103 18-19 0.5632689148465246 1103 20-21 0.3330097278406612 1103 22-23 0.5046749290825474 1103 24-25 0.510951999381632 1103 26-27 0.29744072668236043 1103 28-29 0.6026219954708765 1103 30-31 0.669224834230171 1103 32-33 0.6454737694573822 1103 34-35 0.5269069146280998 1103 36-37 0.8097829897207518 1103 38-39 0.6909151389461812 1103 40-41 0.7314358003081978 1103 42-43 0.957903245830245 1103 44-45 0.7636351838192894 1103 46-47 0.8991565478625603 1103 48-49 0.9252484725542729 1103 50-51 0.9251471015496904 1103 52-53 0.9805498320349315 1103 54-55 0.9642151353310027 1103 56-57 0.8560725036101218 1103 58-59 0.7798936800010239 1103 60-61 0.5821660019211095 1103 62-63 0.9513359829794084 1103 64-65 0.8802725939238947 1103 66-67 0.6458342640196655 1103 68-69 0.992725549713402 1103 70-71 0.6535114687409305 1103 72-73 0.8337539790793578 1103 74-75 0.7850318421330336 1103 76-77 0.6798570739219301 1103 78-79 0.5125591778946372 1103 80-81 0.893493698076707 1103 82-83 0.6687077851433294 1103 84-85 0.918024043903003 1103 86-87 1.2107200606290789 1103 88-89 1.0667939465647471 1103 90-91 1.0273671422180364 1103 92-93 1.2681741821541586 1103 94-95 1.5753395114702613 1103 96-97 1.5575670726993422 1103 98-99 1.586676243682863 1103 100-101 1.4236936448261392 1104 1 0.46718464386699665 1104 2 0.5336193529720639 1104 3 0.44618477191477623 1104 4 0.3804098463840475 1104 5 0.4349121491863599 1104 6 0.41287735653190083 1104 7 0.5119607396589672 1104 8 0.5368573024304695 1104 9 0.6830324522756257 1104 10-11 0.4929083508264327 1104 12-13 0.5051306412124035 1104 14-15 0.6323586537446602 1104 16-17 0.5894633948883907 1104 18-19 0.5151723625663109 1104 20-21 0.5548483406896239 1104 22-23 0.49632043004096005 1104 24-25 0.462545749725237 1104 26-27 0.6565052084859602 1104 28-29 0.8446874509581406 1104 30-31 0.8860001698578301 1104 32-33 0.9659161785731101 1104 34-35 0.8907243654338615 1104 36-37 0.8922342658327622 1104 38-39 1.1229099932051554 1104 40-41 1.1394214723759362 1104 42-43 1.063780654536714 1104 44-45 1.2738270984576516 1104 46-47 1.0358387665907642 1104 48-49 1.1823027923745428 1104 50-51 1.0788458777869252 1104 52-53 1.2527261799515301 1104 54-55 0.9517521066006154 1104 56-57 1.310827977068385 1104 58-59 1.3843860023159777 1104 60-61 1.5328850251587909 1104 62-63 1.4317053230613332 1104 64-65 1.699519277962935 1104 66-67 1.5459144066148447 1104 68-69 1.6255435759332215 1104 70-71 1.5363939388920897 1104 72-73 1.7477506512069603 1104 74-75 1.760806116115642 1104 76-77 1.669883230217092 1104 78-79 1.7377998805594999 1104 80-81 1.7837423233746463 1104 82-83 1.4602600714674132 1104 84-85 1.4905503437462606 1104 86-87 1.696673726969557 1104 88-89 1.7385715362028975 1104 90-91 1.5640045861579246 1104 92-93 1.3924914124456187 1104 94-95 1.55537808009419 1104 96-97 1.5969704135216105 1104 98-99 1.5112970018366987 1104 100-101 0.9150780104229757 1105 1 0.25837795618762627 1105 2 0.427712600253372 1105 3 0.3515875836145739 1105 4 0.2668161738456547 1105 5 0.3174475001030501 1105 6 0.3488154463771238 1105 7 0.38340483995596486 1105 8 0.35797597872113585 1105 9 0.46898032075975493 1105 10-11 0.4574150753462547 1105 12-13 0.5239069923701507 1105 14-15 0.4828573643287797 1105 16-17 0.6332919366886571 1105 18-19 0.5937403150102867 1105 20-21 0.4562307673384609 1105 22-23 0.396038441384178 1105 24-25 0.36473411814796464 1105 26-27 0.38664934107521276 1105 28-29 0.5013710369442137 1105 30-31 0.6013494491948776 1105 32-33 0.5084382487337109 1105 34-35 0.4004418309925839 1105 36-37 0.4848957384502981 1105 38-39 0.4918723659123003 1105 40-41 0.9649712365515484 1105 42-43 1.0118692717462139 1105 44-45 0.9670884792014647 1105 46-47 0.8841593422114826 1105 48-49 1.1464731771980894 1105 50-51 1.2939095299038925 1105 52-53 1.1527571077396956 1105 54-55 1.413567886140065 1105 56-57 1.4757583668039942 1105 58-59 1.3760543745591889 1105 60-61 1.1304646983248432 1105 62-63 1.0961547402089735 1105 64-65 0.9016571613196938 1105 66-67 1.145814118713524 1105 68-69 1.0967689076110112 1105 70-71 1.4862040513691817 1105 72-73 1.3909595001929382 1105 74-75 1.328550252343451 1105 76-77 0.9835371664603656 1105 78-79 0.9462258835522199 1105 80-81 1.060608110418631 1105 82-83 1.2863212811987488 1105 84-85 1.4986657043961067 1105 86-87 1.728685124769136 1105 88-89 1.5092160622118023 1105 90-91 1.3412394599680013 1105 92-93 1.4456653767058292 1105 94-95 1.5981498466621602 1105 96-97 1.431905851763819 1105 98-99 1.3462972519879308 1105 100-101 1.251180290437711 1106 1 0.08906584535899142 1106 2 0.1748663528785528 1106 3 0.10136467362599788 1106 4 0.29641346208116204 1106 5 0.3634148557642263 1106 6 0.2558490855208575 1106 7 0.2967831237332774 1106 8 0.4001704446582792 1106 9 0.3397625321742197 1106 10-11 0.15894781179664363 1106 12-13 0.24986034707336557 1106 14-15 0.37944739417292794 1106 16-17 0.396263087346334 1106 18-19 0.15187376624641757 1106 20-21 0.1202280408361176 1106 22-23 0.20267231633351201 1106 24-25 0.2238494786726335 1106 26-27 0.25634832047906997 1106 28-29 0.524645787321866 1106 30-31 0.25206976163730843 1106 32-33 0.24449992663798525 1106 34-35 0.22018502394789152 1106 36-37 0.35977507229945616 1106 38-39 0.16487078159336477 1106 40-41 0.25203693052008447 1106 42-43 0.28096768374282277 1106 44-45 0.6051195023767306 1106 46-47 0.4230820053410653 1106 48-49 0.7133203825331975 1106 50-51 0.6299435743385047 1106 52-53 0.6984278641598678 1106 54-55 0.5320694151920478 1106 56-57 0.5993481387357633 1106 58-59 0.6718614560963516 1106 60-61 0.8951030683185799 1106 62-63 0.858110821414531 1106 64-65 0.8357697584973955 1106 66-67 0.5074035958882739 1106 68-69 0.5923217612273106 1106 70-71 0.6090808797837575 1106 72-73 0.8169091704335365 1106 74-75 0.4548407353765036 1106 76-77 0.36287470008108613 1106 78-79 0.3780946429936556 1106 80-81 0.4791151661645472 1106 82-83 0.5136342026091612 1106 84-85 0.8712790937332748 1106 86-87 1.064643637720021 1106 88-89 0.8130663256508797 1106 90-91 0.8620014068380506 1106 92-93 0.5012620754273236 1106 94-95 0.8357811056430364 1106 96-97 0.9154270926908339 1106 98-99 0.9675823925501739 1106 100-101 0.8066959309815385 1107 1 0.051486622737886734 1107 2 0.13921949740720763 1107 3 0.1763591335825616 1107 4 0.13472840247029438 1107 5 0.08451577319542025 1107 6 0.11113428695683325 1107 7 0.00885155434976781 1107 8 0.11034196440299127 1107 9 0.04262562662088243 1107 10-11 0.13099053661160553 1107 12-13 0.15419044502133517 1107 14-15 0.4068839052751798 1107 16-17 0.3177806160437555 1107 18-19 0.26785125150990297 1107 20-21 -0.020446958055870823 1107 22-23 0.03701393757182103 1107 24-25 0.11313060263664454 1107 26-27 -0.06316149647789615 1107 28-29 -0.14321309669178106 1107 30-31 0.013612996136266986 1107 32-33 -0.05458485818672898 1107 34-35 0.07508242234349893 1107 36-37 0.0831496235559328 1107 38-39 0.22478081128799943 1107 40-41 0.2800052857919866 1107 42-43 0.5035577648490559 1107 44-45 0.09754072295910277 1107 46-47 0.19475243923726282 1107 48-49 0.14442440238870802 1107 50-51 0.09138930407302581 1107 52-53 0.5083534059761163 1107 54-55 0.26091088130914386 1107 56-57 0.5201853500994957 1107 58-59 0.3776142371981521 1107 60-61 -0.1807977427437777 1107 62-63 -0.3104106517938199 1107 64-65 -0.07553857815996778 1107 66-67 0.047234292160936775 1107 68-69 0.18828860960740457 1107 70-71 0.1906391832008545 1107 72-73 0.30998633215848415 1107 74-75 0.19267923203031856 1107 76-77 -0.2590505758130739 1107 78-79 -0.0500199694249055 1107 80-81 0.297782896519557 1107 82-83 0.19686781516068308 1107 84-85 0.1910060803928495 1107 86-87 0.5630370251241779 1107 88-89 0.48542815696763597 1107 90-91 0.5191569269373275 1107 92-93 0.5009939373251164 1107 94-95 0.5217514996509252 1107 96-97 0.5209693521421457 1107 98-99 0.6284519577675702 1107 100-101 0.7245636790646817 1108 1 -0.05527666228626771 1108 2 0.013583954522911057 1108 3 -0.2937248135149275 1108 4 0.01918631012848948 1108 5 -0.2862492541269859 1108 6 -0.2459932967922711 1108 7 -0.07940377153919798 1108 8 -0.09449291324015263 1108 9 -0.07290854618342735 1108 10-11 -0.011569057908069169 1108 12-13 -0.17655715931857685 1108 14-15 -0.3284748497735279 1108 16-17 -0.020431152388965756 1108 18-19 -0.1395535160516701 1108 20-21 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#Quality Count 2 3.0 3 6.0 4 18.0 5 48.0 6 114.0 7 252.0 8 560.0 9 1064.0 10 1657.0 11 2428.0 12 3541.0 13 4858.0 14 6198.0 15 7983.0 16 9744.0 17 11663.0 18 10547.0 19 8701.0 20 8768.0 21 9524.0 22 10439.0 23 11603.0 24 13440.0 25 15863.0 26 19046.0 27 22466.0 28 27124.0 29 32078.0 30 38078.0 31 45331.0 32 54320.0 33 65488.0 34 80350.0 35 97318.0 36 112393.0 37 93390.0 38 25804.0 39 376.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.09746513237381 20.924094732026603 15.647323785058239 22.33111635054134 2 16.371344048308885 22.109900834473102 38.179666257680225 23.33908885953779 3 20.02362316477685 25.184123450389595 30.613580621967195 24.178672762866356 4 13.622955184421928 18.317043619213614 36.966371602530195 31.093629593834265 5 13.332465341924102 38.487794750528714 34.201442038700684 13.978297868846498 6 31.457428241674723 37.666669794413224 16.53807718652942 14.337824777382638 7 27.832259532547717 33.754174099995424 20.676441713905692 17.737124653551167 8 26.233826860167657 35.22979317851534 19.422077457478615 19.114302503838392 9 26.11590177624727 17.23325795464142 20.36737729068338 36.283462978427934 10-11 25.017476283861768 27.030826288084224 27.279012977020443 20.67268445103356 12-13 26.15525273756017 24.505327334315446 27.694221331857037 21.64519859626735 14-15 22.638552817855427 26.13546396178193 26.45238399635929 24.773599224003352 16-17 21.759323338079945 29.325203099451286 27.09574235987135 21.81973120259742 18-19 21.567365983223674 27.858751856884563 29.682765237596186 20.891116922295573 20-21 22.40081118630132 26.640538085349323 29.77221452335474 21.186436204994614 22-23 22.008108296724018 26.68612517131753 29.571121186370604 21.734645345587854 24-25 22.361146705622865 26.953083077688174 29.58203995747034 21.10373025921862 26-27 22.1589569578563 27.047571907955003 29.645207563369198 21.1482635708195 28-29 21.47852880185413 27.65170352715979 29.759179154194776 21.110588516791307 30-31 21.80098372173354 26.973703887677203 29.63979269944058 21.585519691148676 32-33 21.650560141129056 27.33284616909447 29.469135643179957 21.547458046596514 34-35 22.425826565147297 26.950976419207826 29.301431713992653 21.32176530165222 36-37 22.509065168932054 26.692384788930962 29.301828047101928 21.496721995035053 38-39 22.06275644138533 27.20589442767092 29.488266743613746 21.243082387330006 40-41 22.050860707484958 27.137048746545062 29.37770904550698 21.434381500463004 42-43 21.977654633839666 27.465763828328566 28.79140200135942 21.765179536472353 44-45 22.266240339310283 27.333315348136715 28.612004180385263 21.788440132167736 46-47 22.507401030279713 27.640267703827426 28.17358758785058 21.67874367804228 48-49 22.600783879505652 27.79124384423518 27.94360947012772 21.664362806131454 50-51 22.261417370859803 27.87572655196661 28.036182302598668 21.826673774574918 52-53 22.613740192990914 28.054936844562206 27.578734639253373 21.75258832319351 54-55 22.062087833142247 27.7542056047362 27.965680740573497 22.218025821548064 56-57 22.630686513659715 27.89498040149515 27.660138523862788 21.814194560982347 58-59 22.672592363217518 27.532085793576506 27.633662460501224 22.161659382704755 60-61 22.30869472911298 27.646322624687553 28.181305282616524 21.86367736358294 62-63 22.161068189255477 27.676660219591525 28.248862574083695 21.913409017069306 64-65 21.99601542921522 27.81733610857457 28.004013894973657 22.182634567236555 66-67 22.084828552444144 27.78524206275434 27.671234965460656 22.45869441934086 68-69 22.07857633396848 27.680179506532976 27.826914133265774 22.414330026232772 70-71 22.306735426750627 27.59740278782142 27.711357788792057 22.384503996635893 72-73 22.288774707683537 27.36957117144169 27.869428363479397 22.47222575739537 74-75 21.980998897985348 27.97140744103283 27.400283509251 22.64731015173082 76-77 22.12873391998921 27.48147518246249 27.60879573291421 22.780995164634092 78-79 22.00260282416392 27.684419006598553 27.583191550381187 22.72978661885634 80-81 21.81554801610458 27.992117787467635 27.522938470064894 22.669395726362893 82-83 21.88858418352386 27.71583970047645 27.50365372750582 22.891922388493864 84-85 21.498679278036605 27.753458977859722 27.404863447201617 23.342998296902053 86-87 21.656057679112433 27.723530605374986 27.33001868448554 23.290393031027037 88-89 21.593761436037948 27.5697656961087 27.17660057614173 23.65987229171163 90-91 21.559711318801522 27.657324508283015 27.373100800882117 23.40986337203335 92-93 21.634010310064337 27.80280682411312 27.28114571909473 23.282037146727816 94-95 21.38325092645428 27.58191767678871 27.08495464054043 23.949876756216586 96-97 21.411627130949636 27.66116163402177 27.02362877067173 23.903582464356862 98-99 21.555928933146838 27.82345299987391 26.434605651685622 24.186012415293625 100-101 21.303581073987907 27.758323104234762 26.431775675721642 24.506320146055693 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 218.0 1 156.5 2 119.0 3 151.0 4 149.0 5 150.0 6 212.0 7 391.0 8 584.5 9 718.5 10 718.0 11 667.5 12 740.5 13 838.5 14 938.5 15 1037.0 16 1097.0 17 1195.5 18 1302.5 19 1396.0 20 1572.0 21 1845.0 22 2157.5 23 2541.5 24 2952.5 25 3625.5 26 4766.5 27 6089.0 28 7215.5 29 8608.0 30 10550.0 31 12507.5 32 14764.0 33 16797.0 34 18639.0 35 20591.5 36 22794.5 37 24602.0 38 25366.0 39 25604.5 40 26018.0 41 27269.0 42 28364.5 43 29020.5 44 29753.5 45 30806.5 46 30930.5 47 32274.0 48 37676.0 49 37558.0 50 32342.0 51 29521.5 52 27386.0 53 26418.5 54 25376.0 55 23145.0 56 20852.5 57 18766.0 58 16973.0 59 14987.5 60 12585.5 61 10510.0 62 8730.0 63 6910.5 64 5194.5 65 3919.0 66 2976.0 67 2389.0 68 1817.0 69 1239.0 70 938.5 71 787.0 72 698.5 73 577.0 74 467.5 75 365.0 76 250.5 77 174.0 78 118.0 79 74.0 80 45.0 81 41.5 82 35.5 83 19.0 84 13.0 85 6.5 86 2.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008679496683024781 2 0.008210334700158577 3 0.0036360053672130836 4 1.172904957165511E-4 5 0.004808910324378595 6 0.0 7 5.864524785827555E-4 8 0.0015247764443151643 9 0.0 10-11 0.0 12-13 0.0 14-15 3.518714871496533E-4 16-17 0.005395362802961351 18-19 0.0015834216921734399 20-21 6.450977264410311E-4 22-23 5.864524785827555E-5 24-25 2.9322623929137773E-4 26-27 1.172904957165511E-4 28-29 0.0 30-31 6.450977264410311E-4 32-33 0.0036360053672130836 34-35 5.278072307244799E-4 36-37 8.796787178741332E-4 38-39 0.005864524785827555 40-41 5.864524785827555E-5 42-43 0.0015834216921734399 44-45 0.0036360053672130836 46-47 0.0 48-49 0.0034600696236382574 50-51 0.0014661311964568888 52-53 5.864524785827555E-4 54-55 1.7593574357482666E-4 56-57 0.01120124234093063 58-59 0.0030495528886303287 60-61 0.0038705863586461857 62-63 1.172904957165511E-4 64-65 0.005336717555103075 66-67 6.450977264410311E-4 68-69 0.0028736171450555016 70-71 0.006392332016552035 72-73 0.00475026507652032 74-75 0.006392332016552035 76-77 0.0013488407007403377 78-79 0.005688589042252728 80-81 0.0036946506150713596 82-83 0.003518714871496533 84-85 0.002345809914331022 86-87 5.864524785827555E-4 88-89 0.0018766479314648176 90-91 0.01014562787948167 92-93 0.0022285194186144708 94-95 6.450977264410311E-4 96-97 5.864524785827555E-5 98-99 7.623882221575822E-4 100-101 0.003811941110787911 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 852584.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.743848971912826 #Duplication Level Percentage of deduplicated Percentage of total 1 80.72436818995011 49.03508829683413 2 11.57969232101291 14.06790162977654 3 3.4486601008647173 6.2845466496716424 4 1.4028860219849282 3.408667865770402 5 0.7142910319257071 2.169439328264346 6 0.43161142826897136 1.5730643647993192 7 0.2899611812682998 1.232935074187533 8 0.1923830805129583 0.9348871029944387 9 0.15839482990074405 0.8659360462880357 >10 0.9382651241629211 10.75949975723936 >50 0.07753284367955486 3.198171977010046 >100 0.03828046823876352 4.064934938213582 >500 0.0027066997480140477 1.0133858196637937 >1k 9.666784814335884E-4 1.3915411492868572 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3232 0.37908288215589314 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3091 0.3625449222598594 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2735 0.3207895057847672 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1767 0.20725230593114577 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1024 0.12010546761374832 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 885 0.10380208870914773 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.172904957165511E-4 0.0 14-15 0.0 0.0 0.0 1.172904957165511E-4 0.0 16-17 0.0 0.0 0.0 2.345809914331022E-4 0.0 18-19 0.0 0.0 0.0 3.5187148714965326E-4 0.0 20-21 0.0 0.0 0.0 4.1051673500792885E-4 0.0 22-23 0.0 0.0 0.0 5.864524785827555E-4 0.0 24-25 0.0 0.0 0.0 0.0013488407007403375 0.0 26-27 0.0 0.0 0.0 0.001700712187889991 0.0 28-29 0.0 0.0 0.0 0.004105167350079288 0.0 30-31 0.0 0.0 0.0 0.011318532836647181 0.0 32-33 0.0 0.0 0.0 0.022695710921152638 0.0 34-35 0.0 0.0 0.0 0.040406575774351854 0.0 36-37 0.0 0.0 0.0 0.06486164413125275 0.0 38-39 0.0 0.0 0.0 0.10802454655494356 0.0 40-41 0.0 0.0 0.0 0.1630924342938643 0.0 42-43 0.0 0.0 0.0 0.22384891107503777 0.0 44-45 0.0 0.0 0.0 0.2987975378379139 0.0 46-47 0.0 0.0 0.0 0.40951976579433813 0.0 48-49 0.0 0.0 0.0 0.5205352199900537 0.0 50-51 0.0 0.0 0.0 0.6533667063890478 0.0 52-53 0.0 0.0 0.0 0.7707158473534572 0.0 54-55 0.0 0.0 0.0 0.9021984930517111 0.0 56-57 0.0 0.0 0.0 1.0781342366265378 0.0 58-59 0.0 0.0 0.0 1.2686140016702168 0.0 60-61 0.0 0.0 0.0 1.4742242406613308 0.0 62-63 0.0 0.0 0.0 1.6997738639242586 0.0 64-65 0.0 0.0 0.0 1.9381667964681486 0.0 66-67 0.0 0.0 0.0 2.2147964306156345 0.0 68-69 0.0 0.0 0.0 2.556698225629381 0.0 70-71 0.0 0.0 0.0 2.9529641654077485 0.0 72-73 0.0 0.0 0.0 3.3367973126401624 0.0 74-75 0.0 0.0 0.0 3.7310106687434903 0.0 76-77 0.0 0.0 0.0 4.213133251386374 0.0 78-79 0.0 0.0 0.0 4.726572396385576 0.0 80-81 0.0 0.0 0.0 5.259247182682293 0.0 82-83 0.0 0.0 0.0 5.7942677788933405 0.0 84-85 0.0 0.0 0.0 6.359490677751401 0.0 86-87 0.0 0.0 0.0 6.931809651600311 0.0 88-89 0.0 0.0 0.0 7.525006333686768 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATACG 60 8.2907E-5 39.581913 4 GTAAGGT 345 0.0 27.535246 4 TAAGGTG 400 0.0 26.124063 5 CGTATAG 95 0.0012261313 25.000572 1 GTACATG 3580 0.0 23.087118 1 GGTAAGG 395 0.0 22.848623 3 ACCGTGC 130 2.9413588E-4 21.922293 8 GAGTACT 3755 0.0 21.820124 12-13 TACATGG 3760 0.0 21.602888 2 ACATGGG 3640 0.0 21.401588 3 GGTGTGC 940 0.0 21.222645 8 GTACTTT 4015 0.0 20.821173 14-15 AGTACTT 3935 0.0 20.399523 12-13 AGGTAAG 420 0.0 20.357609 2 ATACGTC 235 1.1314296E-7 20.212042 5 CATGGGG 1510 0.0 19.502613 4 ACTTTTT 4670 0.0 17.90086 16-17 GTAAGCG 80 0.0011916216 17.811863 94-95 TACTTTT 4465 0.0 17.765322 14-15 TAATACG 295 6.330811E-8 17.712269 3 >>END_MODULE