FastQCFastQC Report
Thu 26 May 2016
SRR936402_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936402_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000946
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44370.443280656498952No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33330.33298499619360084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20230.2021088050704034No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17460.1744349845046586No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17090.1707384813965988No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT12810.12797893193039384No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC11630.11619008418036537No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACG1501.0540261E-625.349441
CATTCCG1901.1845259E-825.0121429
GTATCAA70500.024.4729961
TAGCCCT5950.023.962414
GTACATG41200.022.8421841
GAGTACT36250.022.54662512-13
ACATGGG41350.021.9525033
GTACTTT38350.021.37181314-15
TACATGG43700.021.3157622
ATCAACG81250.021.057433
TCAACGC81550.020.9799654
AGTACTT38300.020.96761512-13
CAACGCA81850.020.9051575
GTATAGC4350.020.7603191
TATCAAC83050.020.7154872
AACGCAG83150.020.6323856
GGTATCA51900.019.5981961
ACGCAGA91150.018.768467
CGCAGAG92150.018.5647858
AGAGTAC70050.018.52030610-11
Produced by FastQC (version 0.11.5)