##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936402_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1000946 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00286728754598 33.0 31.0 34.0 31.0 34.0 2 32.123997698177526 33.0 31.0 34.0 30.0 34.0 3 32.15856299940256 33.0 31.0 34.0 30.0 34.0 4 35.79525968433862 37.0 35.0 37.0 35.0 37.0 5 35.758325623959735 37.0 35.0 37.0 35.0 37.0 6 35.84647223726355 37.0 35.0 37.0 35.0 37.0 7 35.72387321593772 37.0 35.0 37.0 35.0 37.0 8 35.7237423397466 37.0 35.0 37.0 35.0 37.0 9 37.380831733180415 39.0 37.0 39.0 35.0 39.0 10-11 37.26841707744474 39.0 37.0 39.0 34.0 39.0 12-13 37.21361042453839 39.0 37.0 39.0 34.0 39.0 14-15 38.449373892297885 40.0 38.0 41.0 34.0 41.0 16-17 38.40588952850603 40.0 38.0 41.0 34.0 41.0 18-19 38.37792847965824 40.0 38.0 41.0 34.0 41.0 20-21 38.28286790696002 40.0 38.0 41.0 34.0 41.0 22-23 38.18073202750198 40.0 38.0 41.0 34.0 41.0 24-25 38.04580566783822 40.0 38.0 41.0 33.5 41.0 26-27 37.88105452242179 40.0 37.5 41.0 33.0 41.0 28-29 37.96538074981068 40.0 38.0 41.0 33.0 41.0 30-31 37.896924009886646 40.0 37.5 41.0 33.0 41.0 32-33 37.74019777290683 40.0 37.0 41.0 33.0 41.0 34-35 37.571576788358215 40.0 37.0 41.0 33.0 41.0 36-37 37.34448012180477 40.0 37.0 41.0 32.5 41.0 38-39 37.18616488801594 39.0 36.0 41.0 32.0 41.0 40-41 37.042508287160345 39.0 36.0 41.0 31.5 41.0 42-43 36.781979747159184 39.0 36.0 41.0 31.0 41.0 44-45 36.51005598703627 39.0 36.0 40.0 30.5 41.0 46-47 36.1672517798163 39.0 35.0 40.0 30.0 41.0 48-49 35.95906622335271 39.0 35.0 40.0 29.5 41.0 50-51 35.29275855041131 38.0 34.5 39.5 28.5 40.5 52-53 35.47990700797046 38.5 35.0 40.0 29.0 40.5 54-55 35.853890719379464 39.0 35.0 40.5 29.0 41.0 56-57 35.7866453335145 39.0 35.0 41.0 29.0 41.0 58-59 35.579057711405014 39.0 35.0 40.5 28.0 41.0 60-61 35.28757944984045 38.0 35.0 40.0 28.0 41.0 62-63 34.98751630957115 38.0 34.5 40.0 27.5 41.0 64-65 34.68199733052532 37.5 34.0 40.0 27.0 41.0 66-67 34.3220598313995 37.0 34.0 40.0 26.0 41.0 68-69 33.93850567363275 36.5 34.0 39.0 26.0 41.0 70-71 33.51277341634813 36.0 33.5 39.0 25.5 40.5 72-73 33.10718560242011 35.5 33.0 38.5 25.0 40.0 74-75 32.684794184701275 35.0 33.0 37.5 24.0 39.5 76-77 32.27415215206415 35.0 32.5 37.0 23.0 39.0 78-79 31.8167263768475 35.0 32.0 36.5 21.5 39.0 80-81 31.406589865986774 35.0 32.0 36.0 20.0 38.5 82-83 31.01973932659704 35.0 31.0 35.5 18.5 37.0 84-85 30.643939333390612 34.0 31.0 35.0 18.0 37.0 86-87 30.336861329182593 34.0 31.0 35.0 14.5 36.0 88-89 29.955024546778745 34.0 30.5 35.0 8.5 36.0 90-91 29.61767467975295 34.0 30.0 35.0 4.5 36.0 92-93 29.26759285715713 34.0 30.0 35.0 2.0 35.0 94-95 28.888277689305916 34.0 29.0 35.0 2.0 35.0 96-97 28.338565716831877 34.0 29.0 35.0 2.0 35.0 98-99 27.747110733246352 34.0 29.0 35.0 2.0 35.0 100-101 25.78704195830744 32.0 23.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2641355416566462 1101 2 0.31591282899464446 1101 3 0.3834265538714057 1101 4 0.3458386130145641 1101 5 0.3590350764328605 1101 6 0.30947200397693564 1101 7 0.2740669897479364 1101 8 0.3016247862830852 1101 9 0.46227878971301806 1101 10-11 0.2940478592773772 1101 12-13 0.46633308218761726 1101 14-15 0.5319367247519224 1101 16-17 0.6038967471067878 1101 18-19 0.6700854915910455 1101 20-21 0.6232106602892387 1101 22-23 0.495355897290203 1101 24-25 0.5152416231658279 1101 26-27 0.6077896151198274 1101 28-29 0.6877524032459021 1101 30-31 0.4732233393399454 1101 32-33 0.579023589185681 1101 34-35 0.5674789012125885 1101 36-37 0.7205779804190229 1101 38-39 0.5431957495162507 1101 40-41 0.4191422811903891 1101 42-43 0.2658701736725675 1101 44-45 0.46430744312758065 1101 46-47 0.5608434003620388 1101 48-49 0.6472383921981759 1101 50-51 0.8083884525005871 1101 52-53 0.9308863116556338 1101 54-55 0.8596849658391861 1101 56-57 0.8794045319403736 1101 58-59 0.9132906524386541 1101 60-61 0.7952421768123017 1101 62-63 0.8618031826261543 1101 64-65 0.8403496972918276 1101 66-67 0.8270213042514669 1101 68-69 0.9517514334980675 1101 70-71 0.9260395549116183 1101 72-73 0.8893659943073899 1101 74-75 0.8238640625925981 1101 76-77 0.872783812986107 1101 78-79 0.8674181316222374 1101 80-81 0.8661154208137276 1101 82-83 0.8796844157739834 1101 84-85 0.8735912423817425 1101 86-87 0.8931842194297452 1101 88-89 0.7802275168509105 1101 90-91 0.8735409765470727 1101 92-93 0.8105933986968097 1101 94-95 0.7894186137331722 1101 96-97 0.8532863359953602 1101 98-99 0.6735047018057365 1101 100-101 0.956657708719721 1102 1 0.19752745150355366 1102 2 0.2431049068038682 1102 3 0.2253252888913586 1102 4 0.24234158564317454 1102 5 0.2813383983647526 1102 6 0.17846932475927701 1102 7 0.026156770011510844 1102 8 0.043182841230034796 1102 9 0.24197186695824513 1102 10-11 0.29053128702742725 1102 12-13 0.3038519731925007 1102 14-15 0.3094223244721732 1102 16-17 0.18226853983659907 1102 18-19 0.17465040167034118 1102 20-21 0.04194977626471541 1102 22-23 -0.15984311966422382 1102 24-25 -0.07188922353479654 1102 26-27 0.05024373855025033 1102 28-29 0.15698760723388006 1102 30-31 0.08705054042140148 1102 32-33 0.01975481492696929 1102 34-35 0.14352757984881492 1102 36-37 0.04546395277593973 1102 38-39 0.07753164687979819 1102 40-41 -0.08034722179904463 1102 42-43 0.10206395995462714 1102 44-45 0.12735104199756364 1102 46-47 0.4780857400536149 1102 48-49 0.4500044918361681 1102 50-51 0.290990736572482 1102 52-53 0.3187309664880047 1102 54-55 0.1672646853250015 1102 56-57 0.39599667550155004 1102 58-59 0.33626734536911584 1102 60-61 -0.049508002230204795 1102 62-63 0.17692860431523627 1102 64-65 0.28742215029931373 1102 66-67 0.40848263027874765 1102 68-69 0.2535351374760566 1102 70-71 0.36714963109701415 1102 72-73 0.3572834645121432 1102 74-75 0.25890587404934706 1102 76-77 0.21454725559850374 1102 78-79 0.24437123247349746 1102 80-81 0.3786106693316924 1102 82-83 0.3789728427013408 1102 84-85 0.2846874073056469 1102 86-87 0.23706201210439204 1102 88-89 0.06755046767422002 1102 90-91 0.09045203609506913 1102 92-93 0.11827131811991975 1102 94-95 0.2501166052551618 1102 96-97 0.2342799798676971 1102 98-99 0.1699733876765812 1102 100-101 0.45286825318963153 1103 1 0.1780697350275382 1103 2 0.28860940260021906 1103 3 0.2619638171816483 1103 4 0.22372359644777617 1103 5 0.3095930093523265 1103 6 0.17770847842102455 1103 7 0.1616946796081109 1103 8 0.13971621162488645 1103 9 0.22402207029578847 1103 10-11 0.34092827936805037 1103 12-13 0.45456830814184457 1103 14-15 0.5518176173249785 1103 16-17 0.564678653124659 1103 18-19 0.3386338642181741 1103 20-21 0.18737649712133475 1103 22-23 0.2213263925593978 1103 24-25 0.35350985560324233 1103 26-27 0.24667345771739946 1103 28-29 0.18488255868662407 1103 30-31 0.08009294915323295 1103 32-33 0.2331638656177759 1103 34-35 0.1951941797379675 1103 36-37 0.48544285942764986 1103 38-39 0.48188798256369836 1103 40-41 0.424345568232404 1103 42-43 0.48650244033204615 1103 44-45 0.36956780933133615 1103 46-47 0.5818886779304862 1103 48-49 0.7513435688707091 1103 50-51 0.2931402422519085 1103 52-53 0.5777717032777474 1103 54-55 0.6633479752529539 1103 56-57 0.6266386036021885 1103 58-59 0.6818213817361496 1103 60-61 0.7983847080021178 1103 62-63 0.6618637007419679 1103 64-65 0.7693016536081387 1103 66-67 0.7531604204103672 1103 68-69 0.5656862249479317 1103 70-71 0.7228033496030619 1103 72-73 0.676290829237594 1103 74-75 0.5683785570364392 1103 76-77 0.5700524984683355 1103 78-79 0.7876629926286824 1103 80-81 0.8180332357920186 1103 82-83 0.7355735498752516 1103 84-85 0.4790759255793162 1103 86-87 0.6413802637354351 1103 88-89 0.690652108063933 1103 90-91 0.8102311402412887 1103 92-93 0.8253616505717893 1103 94-95 0.6648411939056551 1103 96-97 0.7151186929647722 1103 98-99 0.6341116697247813 1103 100-101 0.6119580714546196 1104 1 0.19827241675222496 1104 2 0.23601912239685774 1104 3 0.33459911620753857 1104 4 0.27033602707609816 1104 5 0.1183442706555411 1104 6 0.17040019651005167 1104 7 0.29859701592975796 1104 8 0.26110330558227446 1104 9 0.34362272229252966 1104 10-11 0.30408852309064116 1104 12-13 0.3085463857216979 1104 14-15 0.2822407360427164 1104 16-17 0.04593192766185439 1104 18-19 0.10879773661309144 1104 20-21 0.1494337069245688 1104 22-23 0.12454987123366834 1104 24-25 0.18876182517672646 1104 26-27 0.26822051888090925 1104 28-29 0.2064732803461098 1104 30-31 0.1571015745267701 1104 32-33 0.1777162485106274 1104 34-35 0.20332473432328868 1104 36-37 0.28088737178841683 1104 38-39 0.2711236085581916 1104 40-41 0.18195704803214596 1104 42-43 0.21365162824682216 1104 44-45 -0.03826306446072891 1104 46-47 0.4266574228367688 1104 48-49 0.41212273893319207 1104 50-51 0.26047673369444624 1104 52-53 0.5220130051367136 1104 54-55 0.6371383369559069 1104 56-57 0.701201634804896 1104 58-59 0.6076397734775227 1104 60-61 0.6798452486160826 1104 62-63 0.500392026701526 1104 64-65 0.6744152679210202 1104 66-67 0.5532499244271349 1104 68-69 0.6686061418959639 1104 70-71 0.6884376880980412 1104 72-73 0.5040192463990749 1104 74-75 0.21504022700190006 1104 76-77 0.44375663069172333 1104 78-79 0.5173178078962763 1104 80-81 0.7505595776245961 1104 82-83 0.5291103411198641 1104 84-85 0.5318275070595035 1104 86-87 0.4272279565207775 1104 88-89 0.560239419353568 1104 90-91 0.6920609758837735 1104 92-93 0.4934891246665636 1104 94-95 0.5552030181382008 1104 96-97 0.6382004205620895 1104 98-99 0.8825235191833904 1104 100-101 0.9387380856200238 1105 1 0.1517130211708242 1105 2 0.17388469412551544 1105 3 0.264056413024246 1105 4 0.1885821238063059 1105 5 0.45998325349256675 1105 6 0.27458701279955733 1105 7 0.2694103498237794 1105 8 0.05988191304059143 1105 9 0.29691314318685613 1105 10-11 0.24530018373994977 1105 12-13 0.40762871432725944 1105 14-15 0.38721551522287 1105 16-17 0.33813900471001546 1105 18-19 0.2776841157684231 1105 20-21 0.07128495915479505 1105 22-23 -0.05949277888477411 1105 24-25 -0.012975948382567992 1105 26-27 -0.0742950351260987 1105 28-29 0.10625055817962448 1105 30-31 0.10661663192691861 1105 32-33 0.020134683838598733 1105 34-35 0.12722198676576824 1105 36-37 -0.07640023366545279 1105 38-39 -0.07570471565400538 1105 40-41 -0.25598086209402027 1105 42-43 -0.09030630835169973 1105 44-45 0.016722878361399296 1105 46-47 0.08739133968315826 1105 48-49 0.27415188855403017 1105 50-51 0.15458833807500127 1105 52-53 0.3227157138467476 1105 54-55 0.21465130780628527 1105 56-57 0.3443703369589315 1105 58-59 0.2953724327872038 1105 60-61 0.43214631051372265 1105 62-63 0.21077035902362695 1105 64-65 0.3274482742547633 1105 66-67 0.16684724034718812 1105 68-69 0.48883421059591825 1105 70-71 0.6205647473644618 1105 72-73 0.5098453659171227 1105 74-75 0.3965577914656748 1105 76-77 0.6379424257332644 1105 78-79 0.561384156974853 1105 80-81 0.6454178444266248 1105 82-83 0.7903222921239923 1105 84-85 0.5599571314605214 1105 86-87 0.7007992606544313 1105 88-89 0.6943217679835954 1105 90-91 0.5823398967250419 1105 92-93 0.4294716453067835 1105 94-95 0.3584538406433033 1105 96-97 0.42525394997502985 1105 98-99 0.8011165398546503 1105 100-101 1.0798750549966023 1106 1 0.04724226529528153 1106 2 -0.0663382172732554 1106 3 -0.0411002240782814 1106 4 0.09091819367619536 1106 5 0.19405684084399155 1106 6 0.18729567264661284 1106 7 0.21545089419939245 1106 8 0.1818644574997208 1106 9 0.2600956048084484 1106 10-11 0.2661714949338432 1106 12-13 0.19096976041544167 1106 14-15 0.14407539279034864 1106 16-17 0.12064017014562012 1106 18-19 0.2686887425690969 1106 20-21 0.0869780716220987 1106 22-23 0.07616690880404775 1106 24-25 -0.00376410670454419 1106 26-27 0.1698409275322419 1106 28-29 0.14465624226689044 1106 30-31 0.19815211784325726 1106 32-33 0.17038255729146812 1106 34-35 0.1319395507965524 1106 36-37 0.09965042677647062 1106 38-39 0.21406774529432226 1106 40-41 0.06808225125108436 1106 42-43 0.0850906854636122 1106 44-45 0.05663191046605931 1106 46-47 0.21977672857218522 1106 48-49 0.20391937243181246 1106 50-51 0.4401388114506588 1106 52-53 0.32439419679474923 1106 54-55 0.5241705517688189 1106 56-57 0.40510210268548974 1106 58-59 0.42682802990356805 1106 60-61 0.4705758572679244 1106 62-63 0.41808835718450155 1106 64-65 0.45276733089535526 1106 66-67 0.38169561440471966 1106 68-69 0.4235651291978044 1106 70-71 0.43693215062052815 1106 72-73 0.5441236608613949 1106 74-75 0.37335859188770115 1106 76-77 0.1809795978026898 1106 78-79 0.4878577405764233 1106 80-81 0.18724654286302567 1106 82-83 0.3146609340484794 1106 84-85 0.3395986119344663 1106 86-87 0.2796611137098317 1106 88-89 0.5789819934346632 1106 90-91 0.680290178858467 1106 92-93 0.8614251199241494 1106 94-95 0.6955293514961305 1106 96-97 0.6008205391732524 1106 98-99 0.40526941091698987 1106 100-101 0.7728192052768605 1107 1 0.025641275380767325 1107 2 0.233577824241177 1107 3 0.028821910184110067 1107 4 -0.03942473800337609 1107 5 0.09556579048355474 1107 6 0.3031416329034755 1107 7 0.37540751215896506 1107 8 0.35645413919028357 1107 9 0.30115151362241477 1107 10-11 0.2535149102645349 1107 12-13 0.24937534480628187 1107 14-15 0.35104352834665775 1107 16-17 0.28534640966389446 1107 18-19 0.3376697461765943 1107 20-21 0.5817531738483623 1107 22-23 0.404484368190964 1107 24-25 0.17688063584021307 1107 26-27 0.31134970967357845 1107 28-29 0.1152529756327425 1107 30-31 -0.03412709332385333 1107 32-33 0.3215203936750086 1107 34-35 0.20461179654994766 1107 36-37 0.25065217085175107 1107 38-39 0.2692824884881375 1107 40-41 -0.13786100453754813 1107 42-43 0.23383015662983553 1107 44-45 0.0073842915580257795 1107 46-47 -0.03747332743503762 1107 48-49 -0.14356407141545446 1107 50-51 -0.21487660885473048 1107 52-53 -0.2664359034738766 1107 54-55 -0.11969053357780979 1107 56-57 -0.09086433067010091 1107 58-59 0.0544041002695721 1107 60-61 -0.08272835929052746 1107 62-63 0.014147542682501069 1107 64-65 -0.3765875706261568 1107 66-67 -0.2567113099719265 1107 68-69 -0.11161368562662943 1107 70-71 -0.12685012681097163 1107 72-73 -0.1079877589582523 1107 74-75 -0.21770600589671574 1107 76-77 -0.27501443088556243 1107 78-79 -0.1477865099422928 1107 80-81 -0.03598671725962532 1107 82-83 -0.03408607453789614 1107 84-85 0.2012702023510542 1107 86-87 -0.03879802823863443 1107 88-89 0.0232239278716726 1107 90-91 0.042936399575065565 1107 92-93 0.022597146882990415 1107 94-95 -0.13223641061032865 1107 96-97 0.20490812399539848 1107 98-99 0.239839788437056 1107 100-101 0.28267333525966976 1108 1 -0.174005347100735 1108 2 -0.2517169229654641 1108 3 -0.2966629136525256 1108 4 -0.2097659341162199 1108 5 0.011397336821545423 1108 6 0.001023313703939266 1108 7 -0.12700347212195595 1108 8 -0.15825111360307176 1108 9 0.022216783384116923 1108 10-11 -0.027052439676502615 1108 12-13 0.07787633138337924 1108 14-15 0.023274204473054283 1108 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-0.2692537951877867 2316 48-49 -0.9973683486920208 2316 50-51 -1.1119336824792754 2316 52-53 -1.272466711980833 2316 54-55 -0.9593318762575436 2316 56-57 -0.7091668879492659 2316 58-59 -0.6947467186222767 2316 60-61 -1.043797191639456 2316 62-63 -1.162405052458233 2316 64-65 -0.9671294779842796 2316 66-67 -1.5409544029383255 2316 68-69 -1.212187558974243 2316 70-71 -0.6647414532643197 2316 72-73 -0.5646605916533431 2316 74-75 -0.6330583349246481 2316 76-77 -1.1234558860682746 2316 78-79 -1.1168508962602033 2316 80-81 -1.1985278241158177 2316 82-83 -1.1705324409081612 2316 84-85 -1.289997295106808 2316 86-87 -1.1323012518815645 2316 88-89 -0.9990400322330437 2316 90-91 -1.2827908131637322 2316 92-93 -1.3573140406724526 2316 94-95 -1.4553985014348463 2316 96-97 -1.6770584063695857 2316 98-99 -1.9355647895686126 2316 100-101 -2.4795437846641732 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1804.0 3 585.0 4 494.0 5 508.0 6 544.0 7 636.0 8 791.0 9 978.0 10 1180.0 11 1703.0 12 2317.0 13 3052.0 14 3769.0 15 4847.0 16 6403.0 17 9121.0 18 7515.0 19 4486.0 20 4264.0 21 4277.0 22 4772.0 23 5204.0 24 5951.0 25 6805.0 26 8225.0 27 9715.0 28 12109.0 29 15116.0 30 18966.0 31 25426.0 32 33863.0 33 46926.0 34 68028.0 35 101001.0 36 152194.0 37 212937.0 38 182616.0 39 31803.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.64940194459182 18.718619701506853 14.464437909848554 23.16754044405277 2 15.165015001363761 19.874134411819654 40.85815993262125 24.102690654195335 3 18.991904945556712 24.5424425842627 30.359751333355984 26.105901136824606 4 12.287397884023035 16.41181397152446 38.142753488483656 33.15803465596885 5 13.376153336391905 37.13155956168469 34.89310492000236 14.59918218192104 6 31.367072461218264 36.22810998638465 17.834100737547757 14.570716814849321 7 27.808297347778854 32.85580227715856 21.323102476132608 18.01279789892998 8 25.943773816079158 33.968961771351644 20.4130968573019 19.674167555267292 9 26.11705481429966 16.827891072854246 21.014629603538843 36.04042450930725 10-11 24.59554898989697 26.14513659279634 27.965041199729733 21.294273217576954 12-13 25.767749234899384 23.861994039111583 28.192983262736853 22.17727346325218 14-15 22.685229827027065 25.09349111953024 26.940031286113054 25.281247767329646 16-17 21.721853907574424 28.488817407701085 27.494242949768143 22.29508573495635 18-19 21.44318306734488 27.06051945196718 30.058111065586058 21.43818641510188 20-21 22.36985754493223 25.998861798448385 29.845663267218047 21.785617389401335 22-23 21.82070615225359 26.029372384602777 29.905905651855907 22.244015811287724 24-25 21.982579904547148 26.164961390250703 29.940664173609495 21.91179453159266 26-27 21.64283858849858 26.33067577828398 30.060694161371536 21.965791471845904 28-29 21.344927872151025 26.655134070180665 30.184048650706192 21.815889406962118 30-31 21.489681204365706 26.363833460553188 30.006584126958753 22.139901208122357 32-33 21.287452669017814 26.55815091964473 29.933611741080796 22.22078467025666 34-35 22.146636847686924 26.408379819337746 29.551661282499197 21.893322050476133 36-37 22.0140204026682 26.16444124953729 29.69686736467284 22.124670983121668 38-39 21.718195033494688 26.435525849106554 29.79928165010665 22.0469974672921 40-41 21.749134294717447 26.46520494186337 29.916986206662283 21.868674556756897 42-43 21.636148564943213 26.708251076991935 29.170696228330286 22.484904129734566 44-45 21.77105292078367 26.6542615367708 29.192101345748455 22.38258419669707 46-47 21.947939583220936 26.84925977656422 28.82760157216304 22.375199068051803 48-49 22.023564813727877 26.928696632120857 28.599373689671175 22.44836486448009 50-51 21.837178772456706 27.08906552532355 28.707017301978365 22.366738400241378 52-53 22.15280958820147 27.197275357077384 28.36487956053244 22.285035494188705 54-55 21.620486188816777 26.842939751910226 28.67224215927619 22.864331899996802 56-57 21.988895355081386 27.23550289355176 28.312748362152295 22.46285338921456 58-59 22.14310013492661 26.766721832883828 28.445686419769125 22.644491612420442 60-61 21.996882914474966 26.779860830123837 28.959472892845188 22.263783362556012 62-63 21.905290458711562 26.895279808658714 28.825427051134238 22.374002681495483 64-65 21.66393832839868 26.890308812590884 28.77103888493972 22.674713974070713 66-67 21.635385820415372 27.082259334300435 28.329796629541264 22.952558215742926 68-69 21.626320135599116 26.923447746334144 28.73773031342558 22.712501804641164 70-71 21.793980267266143 26.84785812414138 28.447708255276634 22.910453353315848 72-73 21.751640895732496 26.636487206783084 28.66965430664256 22.942217590841857 74-75 21.507449176554278 27.212723145598634 28.299477678446543 22.98034999940055 76-77 21.723499210228958 26.77978226097312 28.420817585368706 23.075900943429215 78-79 21.75425166073861 26.814869744325705 28.53647128898501 22.894407305950676 80-81 21.746766039151773 27.030171976619457 28.305120227749985 22.91794175647879 82-83 21.81685120752917 27.04945262174577 28.25525835304461 22.878437817680457 84-85 21.591184983659428 26.9020954497179 28.17991713317141 23.326802433451263 86-87 21.668410130674875 26.946959728479207 28.360764166769076 23.023865974076838 88-89 21.761831708714592 26.77146552383027 27.995012496234644 23.471690271220496 90-91 21.662118942876525 26.826491431928517 28.28108030337697 23.230309321817984 92-93 21.853253497800527 26.951686513140636 28.209970448862542 22.98508954019629 94-95 21.69628704920645 26.71069635748472 28.1199561801171 23.473060413191725 96-97 21.788772787650295 26.67782996977216 28.097204661611542 23.436192580966004 98-99 21.801126416014455 26.91121979102545 27.52819037949587 23.75946341346423 100-101 21.981815755713622 26.620682141548848 27.719403107403785 23.678098995333745 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 109.0 1 92.5 2 128.0 3 176.5 4 157.0 5 163.5 6 267.0 7 543.5 8 937.0 9 1163.5 10 1060.0 11 896.5 12 947.5 13 1035.5 14 1134.5 15 1227.0 16 1263.0 17 1319.0 18 1446.0 19 1589.5 20 1716.5 21 1955.5 22 2292.0 23 2769.0 24 3282.0 25 4029.0 26 5325.5 27 6909.5 28 8285.5 29 9804.0 30 11864.0 31 14299.0 32 17178.0 33 19415.5 34 21456.5 35 23685.5 36 26117.0 37 28524.0 38 29416.5 39 29289.0 40 29838.0 41 31428.5 42 32697.0 43 34070.5 44 35044.5 45 35764.0 46 36174.5 47 37807.5 48 44666.5 49 44368.5 50 37514.0 51 34499.0 52 32172.5 53 31542.5 54 30877.5 55 28515.0 56 25788.5 57 22922.5 58 20573.0 59 18116.0 60 15219.5 61 12624.0 62 10467.5 63 8248.5 64 6158.5 65 4812.0 66 3701.5 67 2912.5 68 2204.5 69 1505.0 70 1126.0 71 958.0 72 812.0 73 696.0 74 561.0 75 457.0 76 325.0 77 177.0 78 109.0 79 80.0 80 59.0 81 39.5 82 24.5 83 20.5 84 13.5 85 7.5 86 6.0 87 6.0 88 4.0 89 3.5 90 3.5 91 1.5 92 2.0 93 2.0 94 0.5 95 0.5 96 0.5 97 0.0 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011888753239435494 2 0.0036965031080597754 3 0.008791683067817845 4 0.036765220101783715 5 0.04785472942596304 6 0.06034291560184066 7 0.03127041818439756 8 0.030870796226769474 9 0.0166842167309725 10-11 0.04535709219078752 12-13 0.04235992750857689 14-15 0.018232751816781325 16-17 0.021629538456620037 18-19 0.027573915076337784 20-21 0.024426892160016627 22-23 0.014186579495796976 24-25 0.0032968811504316916 26-27 0.002597542724582545 28-29 0.0014985823411053144 30-31 0.005394896427979132 32-33 0.0015984878305123356 34-35 0.0017483460646228665 36-37 0.004745510746833496 38-39 0.004096125065687859 40-41 0.002797353703396587 42-43 9.990548940702097E-4 44-45 0.0015984878305123356 46-47 0.003196975661024671 48-49 4.495747023315943E-4 50-51 0.0019981097881404193 52-53 7.492911705526572E-4 54-55 0.0017982988093263772 56-57 0.001348724106994783 58-59 0.00314702291632116 60-61 0.0010989603834772306 62-63 0.0011988658728842513 64-65 0.00314702291632116 66-67 0.0013986768516982934 68-69 0.005444849172682642 70-71 0.007093289747898488 72-73 0.004445794278612433 74-75 0.0029971646822106288 76-77 0.002297826256361482 78-79 0.033668149930166064 80-81 0.019931145136700683 82-83 0.003846361342170307 84-85 0.00629404583264232 86-87 0.006493856811456362 88-89 0.004445794278612433 90-91 0.007892533663154655 92-93 0.013836910282872403 94-95 0.0030471174269141393 96-97 0.004745510746833496 98-99 0.024976372351755243 100-101 0.04640609982956123 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1000946.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.66000705794339 #Duplication Level Percentage of deduplicated Percentage of total 1 79.0116306529702 46.34822811762847 2 12.454743983738107 14.611907399819104 3 3.8705388821118265 6.811375144281722 4 1.5747774402750387 3.695058230448952 5 0.797551017245769 2.339217415035337 6 0.4905437384592643 1.7265179496150225 7 0.3015922055643489 1.2383980634917795 8 0.22288036100879308 1.0459330839882217 9 0.16824304595571626 0.8882224436890988 >10 0.9765545480211567 11.054841731487683 >50 0.0828725151209164 3.2774948230535754 >100 0.04551460931874209 4.815496498622756 >500 0.0013637334454616311 0.5781755243309538 >1k 0.0011932667647789274 1.5691335745073205 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4437 0.443280656498952 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3333 0.33298499619360084 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2023 0.2021088050704034 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1746 0.1744349845046586 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1709 0.1707384813965988 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1281 0.12797893193039384 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1163 0.11619008418036537 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 4.995274470351048E-5 0.0 22-23 0.0 0.0 0.0 1.9981097881404192E-4 0.0 24-25 0.0 0.0 0.0 2.997164682210629E-4 0.0 26-27 0.0 0.0 0.0 6.993384258491467E-4 0.0 28-29 0.0 0.0 0.0 0.0018982042987333984 0.0 30-31 0.0 0.0 0.0 0.005744565640903705 0.0 32-33 0.0 0.0 0.0 0.014486295964018039 0.0 34-35 0.0 0.0 0.0 0.027573915076337784 0.0 36-37 0.0 0.0 0.0 0.04865397334121921 0.0 38-39 0.0 0.0 0.0 0.07922505309976763 0.0 40-41 0.0 0.0 0.0 0.11604022594625485 0.0 42-43 0.0 0.0 0.0 0.16279599498874064 0.0 44-45 0.0 0.0 0.0 0.22069122610010927 0.0 46-47 0.0 0.0 0.0 0.2998663264551734 0.0 48-49 0.0 0.0 0.0 0.3863345275369501 0.0 50-51 0.0 0.0 0.0 0.4766990427056005 0.0 52-53 0.0 0.0 0.0 0.572058832344602 0.0 54-55 0.0 0.0 0.0 0.6824044453946567 0.0 56-57 0.0 0.0 0.0 0.8222221778197825 0.0 58-59 0.0 0.0 0.0 0.9785243159970668 0.0 60-61 0.0 0.0 0.0 1.1525097257993937 0.0 62-63 0.0 0.0 0.0 1.338383888841156 0.0 64-65 0.0 0.0 0.0 1.5339988371001034 0.0 66-67 0.0 0.0 0.0 1.7624327386292569 0.0 68-69 0.0 0.0 0.0 2.035424488433941 0.0 70-71 0.0 0.0 0.0 2.361416100369051 0.0 72-73 0.0 0.0 0.0 2.6842107366431356 0.0 74-75 0.0 0.0 0.0 3.015996866963852 0.0 76-77 0.0 0.0 0.0 3.4147696279319764 0.0 78-79 0.0 0.0 0.0 3.8417157369128807 0.0 80-81 0.0 0.0 0.0 4.310672104189436 0.0 82-83 0.0 0.0 0.0 4.770636977419361 0.0 84-85 0.0 0.0 0.0 5.278256769096435 0.0 86-87 0.0 0.0 0.0 5.8023110137809635 0.0 88-89 0.0 0.0 0.0 6.338403869939038 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTACG 150 1.0540261E-6 25.34944 1 CATTCCG 190 1.1845259E-8 25.012142 9 GTATCAA 7050 0.0 24.472996 1 TAGCCCT 595 0.0 23.96241 4 GTACATG 4120 0.0 22.842184 1 GAGTACT 3625 0.0 22.546625 12-13 ACATGGG 4135 0.0 21.952503 3 GTACTTT 3835 0.0 21.371813 14-15 TACATGG 4370 0.0 21.315762 2 ATCAACG 8125 0.0 21.05743 3 TCAACGC 8155 0.0 20.979965 4 AGTACTT 3830 0.0 20.967615 12-13 CAACGCA 8185 0.0 20.905157 5 GTATAGC 435 0.0 20.760319 1 TATCAAC 8305 0.0 20.715487 2 AACGCAG 8315 0.0 20.632385 6 GGTATCA 5190 0.0 19.598196 1 ACGCAGA 9115 0.0 18.76846 7 CGCAGAG 9215 0.0 18.564785 8 AGAGTAC 7005 0.0 18.520306 10-11 >>END_MODULE