FastQCFastQC Report
Thu 26 May 2016
SRR936402_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936402_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000946
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36250.36215739910045097No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35860.3582610850135772No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32090.32059671550713026No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21190.21169973205347742No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10880.1086971724748388No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACG600.003951767531.6649349
GAGTACT42050.023.1522212-13
GTACATG41800.023.1510851
GTAAGGT4000.022.564654
TACATGG42750.022.3332272
ACATGGG40550.022.1414433
ACCGTTC1302.9422287E-421.9218778
GTACTTT43800.021.84769814-15
TAAGGTG4700.021.2222445
CATGGGG18000.020.8492074
AGTACTT44200.020.3069312-13
ACGCATT1507.7229436E-418.998965
ACTTTTT52550.018.5261816-17
GTATTAC2101.8170378E-518.250171
GTATCAA93650.018.160041
CTATAGA4850.017.7798061
AGAGTAC76000.017.62139510-11
TACTTTT51350.017.47938314-15
GTGTAAG3601.7007551E-917.299641
GGTAAGG4950.017.274373
Produced by FastQC (version 0.11.5)