##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936402_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1000946 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.06354089031776 31.0 31.0 33.0 30.0 34.0 2 31.309422286516956 31.0 31.0 34.0 30.0 34.0 3 31.502669474676956 31.0 31.0 34.0 30.0 34.0 4 35.38222741286743 37.0 35.0 37.0 33.0 37.0 5 35.147593376665675 37.0 35.0 37.0 32.0 37.0 6 35.266202172744585 37.0 35.0 37.0 32.0 37.0 7 35.18995730039383 37.0 35.0 37.0 32.0 37.0 8 35.152091121798776 37.0 35.0 37.0 32.0 37.0 9 36.702838115143074 39.0 35.0 39.0 32.0 39.0 10-11 36.54596102087425 38.0 35.0 39.0 32.0 39.0 12-13 36.40621771803873 38.0 35.0 39.0 32.0 39.0 14-15 37.366737566262316 39.0 36.0 40.0 32.0 41.0 16-17 37.25115990273201 39.0 36.0 40.0 32.0 41.0 18-19 37.300288926675364 39.0 36.0 40.0 32.0 41.0 20-21 37.36947897289164 39.0 36.0 40.0 32.0 41.0 22-23 37.27024834506557 39.0 36.0 40.0 31.5 41.0 24-25 37.12772367340496 39.0 36.0 40.0 31.0 41.0 26-27 36.83800474750886 39.0 36.0 40.0 30.5 41.0 28-29 36.71251096462746 39.0 35.5 40.0 30.0 41.0 30-31 36.69947479684218 39.0 35.5 40.0 30.0 41.0 32-33 36.504047670903326 38.0 35.0 40.0 30.0 41.0 34-35 36.30434758718253 38.0 35.0 40.0 30.0 41.0 36-37 36.04944022954285 38.0 35.0 40.0 30.0 41.0 38-39 35.81309880852713 38.0 35.0 40.0 29.0 41.0 40-41 35.544329564232235 38.0 34.0 40.0 28.0 41.0 42-43 35.27072089803046 38.0 34.0 40.0 27.0 41.0 44-45 34.95325572008879 38.0 33.5 40.0 26.0 41.0 46-47 34.51297922165631 38.0 33.0 40.0 24.5 41.0 48-49 33.91452086326335 37.5 33.0 40.0 23.0 41.0 50-51 33.744295896082306 37.0 33.0 40.0 23.0 41.0 52-53 33.42886379485007 37.0 32.0 40.0 22.5 41.0 54-55 33.11262295868109 37.0 31.5 39.5 20.5 40.5 56-57 32.79224403714086 36.0 31.0 39.0 20.0 40.0 58-59 32.33599414953454 36.0 31.0 39.0 18.0 40.0 60-61 31.902237982868208 35.5 30.0 39.0 15.5 40.0 62-63 31.537145860016423 35.0 30.0 38.5 13.5 40.0 64-65 31.03549542133142 35.0 29.0 38.0 11.0 40.0 66-67 30.558278368663245 34.0 29.0 38.0 8.0 40.0 68-69 30.020980152775472 34.0 28.0 37.0 7.5 39.0 70-71 29.46081357036244 34.0 27.5 36.5 7.0 39.0 72-73 28.950666169803366 33.0 26.0 36.0 3.5 39.0 74-75 28.485026165247675 33.0 26.0 35.5 2.0 38.0 76-77 27.326701440437347 31.5 25.5 34.0 2.0 36.0 78-79 28.123397765713634 32.5 26.0 35.0 2.0 37.0 80-81 27.905367522323882 33.0 26.0 35.0 2.0 36.5 82-83 27.490004955312273 32.5 26.0 35.0 2.0 36.0 84-85 26.933947485678548 32.0 25.0 35.0 2.0 36.0 86-87 26.419283857470834 32.0 24.5 34.0 2.0 35.0 88-89 25.857586223432634 31.5 23.5 34.0 2.0 35.0 90-91 25.21591124795943 31.0 20.5 34.0 2.0 35.0 92-93 24.532085646977958 31.0 18.5 34.0 2.0 35.0 94-95 23.737993358283063 30.5 11.0 34.0 2.0 35.0 96-97 22.74426142868846 30.0 2.0 34.0 2.0 35.0 98-99 21.506646212682803 29.0 2.0 34.0 2.0 35.0 100-101 18.660053589304518 24.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5935898314372707 1101 2 0.5641255633431363 1101 3 0.30688245642265244 1101 4 0.2367807537256965 1101 5 0.39381119024124445 1101 6 0.297336418607415 1101 7 0.42416052319402553 1101 8 0.3295484402520543 1101 9 0.5217891477834726 1101 10-11 0.4416945533425505 1101 12-13 0.4731253219659166 1101 14-15 0.5815339207994228 1101 16-17 0.475243746121933 1101 18-19 0.3884677140956896 1101 20-21 0.3068201682254639 1101 22-23 0.36907978490091153 1101 24-25 0.4451123984838219 1101 26-27 0.4166218782702842 1101 28-29 0.4457872995509291 1101 30-31 0.38259992606177207 1101 32-33 0.4514291229138294 1101 34-35 0.6460127772776545 1101 36-37 0.7137568023696801 1101 38-39 0.6938197088187366 1101 40-41 0.8255387098749623 1101 42-43 0.9616732925710849 1101 44-45 0.9876670818655029 1101 46-47 1.090091942375608 1101 48-49 1.151581205983824 1101 50-51 1.0859076079272825 1101 52-53 1.1103854707725134 1101 54-55 1.0211228381560673 1101 56-57 0.9268625660162684 1101 58-59 0.9525433059485842 1101 60-61 1.1184179337213749 1101 62-63 0.840086425598777 1101 64-65 0.965713425392611 1101 66-67 0.7887689312682866 1101 68-69 0.6597349452770338 1101 70-71 0.6768571165972723 1101 72-73 0.6648876346859538 1101 74-75 0.8295881081217118 1101 76-77 1.0737586905264855 1101 78-79 1.0743413989505868 1101 80-81 0.9298141809593652 1101 82-83 1.0764916023715898 1101 84-85 1.183030063801855 1101 86-87 1.0123085781971604 1101 88-89 0.9376018977420202 1101 90-91 0.8652344840041657 1101 92-93 0.9194961995587008 1101 94-95 0.7584990095071298 1101 96-97 1.0455141451024694 1101 98-99 1.0099022852298596 1101 100-101 0.8374272017288824 1102 1 0.5455114908719629 1102 2 0.7312807198781144 1102 3 0.35674456437188695 1102 4 0.27935894671369965 1102 5 0.23118844824537632 1102 6 0.3538577345721734 1102 7 0.28737712514966063 1102 8 0.2613835759518466 1102 9 0.3743495902807865 1102 10-11 0.38073767539992076 1102 12-13 0.4345917933845769 1102 14-15 0.4968793135183276 1102 16-17 0.6088091345989852 1102 18-19 0.4412901657558095 1102 20-21 0.5182423347490968 1102 22-23 0.4400172320956628 1102 24-25 0.4481232291681039 1102 26-27 0.18498185847494852 1102 28-29 0.2544381037442136 1102 30-31 0.32651840949332467 1102 32-33 0.5259690573964733 1102 34-35 0.7850935307557094 1102 36-37 0.7055466852557544 1102 38-39 0.5329059152069533 1102 40-41 0.8184061765765378 1102 42-43 0.7429428790597825 1102 44-45 0.6590673448099054 1102 46-47 0.8839816078110374 1102 48-49 0.8857384412822924 1102 50-51 0.7366495841443523 1102 52-53 0.6946247691985548 1102 54-55 0.8716219538018279 1102 56-57 0.6520918497923702 1102 58-59 0.7253852137189547 1102 60-61 0.5983148451867208 1102 62-63 0.6771294550834845 1102 64-65 0.7997963489386883 1102 66-67 0.7782357793181944 1102 68-69 0.7302814156431623 1102 70-71 0.5943361981877082 1102 72-73 0.6377671738770019 1102 74-75 0.04091270876737596 1102 76-77 0.5160478000187005 1102 78-79 0.8115517883929932 1102 80-81 0.8317920973072894 1102 82-83 0.8346585241942925 1102 84-85 0.8660570725097081 1102 86-87 0.6383494262007758 1102 88-89 0.653032191003966 1102 90-91 0.7315499716322407 1102 92-93 0.845841570402559 1102 94-95 0.5594616066648292 1102 96-97 0.7887364472513916 1102 98-99 0.5060422906003375 1102 100-101 0.3667564081814483 1103 1 0.5494321658591055 1103 2 0.6459853796132755 1103 3 0.35535600937058476 1103 4 0.18864586299472563 1103 5 0.14084284955153947 1103 6 0.2389605469073146 1103 7 0.32675102749421825 1103 8 0.2672798220537089 1103 9 0.21722042252898888 1103 10-11 0.27939423695877963 1103 12-13 0.2823114037711534 1103 14-15 0.26428685587197265 1103 16-17 0.26079486841712196 1103 18-19 0.29372228640942666 1103 20-21 0.2692591267905513 1103 22-23 0.17736706615352915 1103 24-25 0.05181921867736605 1103 26-27 0.18735204298122454 1103 28-29 0.29906750104373003 1103 30-31 0.2079634833158721 1103 32-33 0.047772542848996125 1103 34-35 0.17042860364336576 1103 36-37 0.28798812137304 1103 38-39 0.3584701017566161 1103 40-41 0.42327098131908514 1103 42-43 0.2441779501110588 1103 44-45 0.5175596295024931 1103 46-47 0.4200351024454605 1103 48-49 0.6485039027817194 1103 50-51 0.4552043642459864 1103 52-53 0.34861695873318155 1103 54-55 0.4117487726544553 1103 56-57 0.29310781128845065 1103 58-59 0.2144415263646131 1103 60-61 0.3587813863116551 1103 62-63 0.3311928044100334 1103 64-65 0.4709619421567659 1103 66-67 0.4023345958536382 1103 68-69 0.22900220882651467 1103 70-71 0.2525653168580355 1103 72-73 0.23403771213718016 1103 74-75 0.1908339739008298 1103 76-77 0.09603881426902561 1103 78-79 0.2320548234311559 1103 80-81 0.35991482269810504 1103 82-83 0.46213273555181544 1103 84-85 0.48029493653065103 1103 86-87 0.2320259709316943 1103 88-89 0.3451065552820012 1103 90-91 0.20973114821870453 1103 92-93 0.26449225971348156 1103 94-95 0.23213251119969058 1103 96-97 0.04658937895134585 1103 98-99 0.24594573865745062 1103 100-101 0.026590795764835917 1104 1 0.3131173057667418 1104 2 0.530976356444107 1104 3 0.29033825410222036 1104 4 0.037973491356581235 1104 5 0.06478990392341188 1104 6 0.1682099259580383 1104 7 0.24039905598026223 1104 8 0.07638764407412424 1104 9 0.06061507460334781 1104 10-11 0.21824164837647686 1104 12-13 0.15774861512677063 1104 14-15 0.36612556914864314 1104 16-17 0.20865877865114157 1104 18-19 0.1283678602053655 1104 20-21 0.09112369678317833 1104 22-23 0.19118803871533174 1104 24-25 0.29540779612138124 1104 26-27 0.3407968561143022 1104 28-29 0.20669462585453857 1104 30-31 0.03153505742228191 1104 32-33 0.2666001615085207 1104 34-35 0.3318244782116153 1104 36-37 0.4771132620568679 1104 38-39 0.37648005634970616 1104 40-41 0.5620488997806845 1104 42-43 0.4318859438627598 1104 44-45 0.8428466972635817 1104 46-47 0.845092467486019 1104 48-49 0.892017287149315 1104 50-51 0.7452440710415047 1104 52-53 0.7518562036976704 1104 54-55 0.671935008925665 1104 56-57 0.8590387985977941 1104 58-59 1.0090219247423882 1104 60-61 0.575342297554716 1104 62-63 0.3653638128704664 1104 64-65 0.6178128076048175 1104 66-67 0.7951462590274687 1104 68-69 0.766969861250157 1104 70-71 0.6429980893965528 1104 72-73 0.7877213239274568 1104 74-75 0.46001609153191225 1104 76-77 0.671858297522796 1104 78-79 0.8620321199732466 1104 80-81 0.7547306239167177 1104 82-83 0.8193623072908629 1104 84-85 0.7360975377259784 1104 86-87 0.9256105461635826 1104 88-89 0.7223011016009373 1104 90-91 0.6829242100807775 1104 92-93 0.9645840680342026 1104 94-95 0.9882055649118051 1104 96-97 0.7052667812488558 1104 98-99 1.3177514882596526 1104 100-101 1.4242560507421338 1105 1 0.43146192288886454 1105 2 0.5182578143857093 1105 3 0.2441693356839103 1105 4 0.13305618240504202 1105 5 -0.006902704444016194 1105 6 0.21388474058150564 1105 7 0.2038338733270635 1105 8 0.21829455431844025 1105 9 0.16253261784117967 1105 10-11 0.30878929760383755 1105 12-13 0.1566672937520437 1105 14-15 0.45987387958398784 1105 16-17 0.31791103490829187 1105 18-19 0.23889066907781142 1105 20-21 0.17250063940706184 1105 22-23 0.24906376285022702 1105 24-25 0.12215883589198029 1105 26-27 0.18265010077929134 1105 28-29 0.11956189966312536 1105 30-31 0.2778235219259102 1105 32-33 0.3869985383249954 1105 34-35 0.30313842385319134 1105 36-37 0.25730240318732456 1105 38-39 0.25670193748845804 1105 40-41 0.3679341916072971 1105 42-43 0.5355844133925203 1105 44-45 0.4573466161644788 1105 46-47 0.633698644234002 1105 48-49 0.6046245932773999 1105 50-51 0.6081935953602127 1105 52-53 0.6143041150453357 1105 54-55 0.7061222857779725 1105 56-57 0.6852446721753154 1105 58-59 0.6739271889752736 1105 60-61 0.8269232294534987 1105 62-63 0.6710641286563579 1105 64-65 0.5949320973769225 1105 66-67 0.7310043776484214 1105 68-69 0.33899938132368845 1105 70-71 0.5200653656080831 1105 72-73 0.5112737549982249 1105 74-75 0.3366886353804901 1105 76-77 0.31271267156787985 1105 78-79 0.4904788318551674 1105 80-81 0.2573410638743461 1105 82-83 0.5415325493266288 1105 84-85 0.9382230350837482 1105 86-87 0.6809030135587406 1105 88-89 0.568024075074522 1105 90-91 0.4375736416861997 1105 92-93 0.6420266528728753 1105 94-95 0.9852872487294313 1105 96-97 0.686959659946627 1105 98-99 0.7393657008274097 1105 100-101 1.0795863643854062 1106 1 0.2674486995624257 1106 2 0.31162075447081605 1106 3 0.008337155002614338 1106 4 -0.02922614532993606 1106 5 -0.1132807691987665 1106 6 -0.041200351132097524 1106 7 -0.21101603475906217 1106 8 -0.2253158331004741 1106 9 -0.03730528647123066 1106 10-11 -0.13180885423260946 1106 12-13 0.17800563142753134 1106 14-15 0.051806934576362096 1106 16-17 -0.025107558370450533 1106 18-19 -0.14569370718191266 1106 20-21 -0.14455238940454507 1106 22-23 -0.16864114288797794 1106 24-25 -0.14030791616895044 1106 26-27 -0.05910811756582035 1106 28-29 -0.111028164433705 1106 30-31 -0.2856613989991743 1106 32-33 -0.28799449462849935 1106 34-35 -0.44199252679285195 1106 36-37 -0.18556270200868852 1106 38-39 -0.25094903010007386 1106 40-41 -0.18556462932129136 1106 42-43 -0.35040487738344694 1106 44-45 -0.18473383340816696 1106 46-47 0.07406328282965546 1106 48-49 -0.36381404685738516 1106 50-51 -0.0797777455767843 1106 52-53 0.2679865080617603 1106 54-55 -0.2001515502565283 1106 56-57 -0.29312893366967785 1106 58-59 -0.3669022602828953 1106 60-61 0.06302258678073969 1106 62-63 -0.5165850662769174 1106 64-65 -0.42083345532931915 1106 66-67 -0.18983207700697236 1106 68-69 0.14109051796216932 1106 70-71 -0.0042144718871526266 1106 72-73 0.031252346777023376 1106 74-75 -0.004349356541993643 1106 76-77 -0.2780153187438792 1106 78-79 -0.2537858187570379 1106 80-81 -0.06952633895559757 1106 82-83 -0.07653012596809816 1106 84-85 0.1966476218121862 1106 86-87 0.28954036153011486 1106 88-89 0.2873343417312988 1106 90-91 0.09639460435731806 1106 92-93 0.571134078338261 1106 94-95 0.546421270841595 1106 96-97 0.5081809853302843 1106 98-99 0.4085238855943345 1106 100-101 0.7189336863549975 1107 1 0.42918289244082075 1107 2 0.4523653259580094 1107 3 0.18009611668111702 1107 4 0.04101004810539166 1107 5 -0.1504255944457782 1107 6 0.027141018626515745 1107 7 0.032268856691622716 1107 8 -0.06147744376341535 1107 9 0.17401675608588363 1107 10-11 -0.07423447978614206 1107 12-13 0.1534514384235095 1107 14-15 0.16196109575572137 1107 16-17 0.15745608572094483 1107 18-19 0.12896988310068735 1107 20-21 -0.01661933844530239 1107 22-23 0.0058658365537027635 1107 24-25 0.2412509479418361 1107 26-27 -0.010962846354793498 1107 28-29 0.3953221120430612 1107 30-31 0.21573930491211257 1107 32-33 -0.09953491299719275 1107 34-35 -0.06983240686411563 1107 36-37 0.188879581243377 1107 38-39 0.40688559471436747 1107 40-41 0.4616924825486777 1107 42-43 0.2830116467352539 1107 44-45 -0.002152392630300426 1107 46-47 0.465596666272873 1107 48-49 0.5306055403851104 1107 50-51 0.43062284736693357 1107 52-53 -0.024570080166888886 1107 54-55 -0.1361986340077337 1107 56-57 0.7737206550174918 1107 58-59 -0.12427479936755859 1107 60-61 0.2197527701703592 1107 62-63 -0.20359578825707914 1107 64-65 -0.3841199380131428 1107 66-67 -0.6195362123287893 1107 68-69 -0.27257670912564436 1107 70-71 -0.3585527806156925 1107 72-73 0.14268054133458818 1107 74-75 0.6541939951393729 1107 76-77 0.27555689497548386 1107 78-79 0.1440266544170754 1107 80-81 0.24408791681837982 1107 82-83 0.4305299242394973 1107 84-85 0.5558559414490496 1107 86-87 0.9678087229996599 1107 88-89 1.0104493062321431 1107 90-91 0.8328832553056706 1107 92-93 0.7547916952153813 1107 94-95 1.3313702990378324 1107 96-97 1.52478843439512 1107 98-99 1.2957515177766084 1107 100-101 1.7624748500907934 1108 1 0.17776335004135646 1108 2 0.19253944611415008 1108 3 -0.06952540354274106 1108 4 -0.06449727046755527 1108 5 0.00126987819665203 1108 6 -0.1864149788671483 1108 7 -0.3497758292188138 1108 8 -0.36960862932942007 1108 9 -0.1742858253602364 1108 10-11 -0.4404744729365291 1108 12-13 -0.24502646964384667 1108 14-15 -0.15568365086715374 1108 16-17 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54.0 6 140.0 7 329.0 8 783.0 9 1260.0 10 2074.0 11 3024.0 12 4168.0 13 5644.0 14 7205.0 15 9007.0 16 11218.0 17 12955.0 18 11585.0 19 9536.0 20 9530.0 21 9930.0 22 10754.0 23 12244.0 24 14388.0 25 17244.0 26 20895.0 27 25027.0 28 30159.0 29 36132.0 30 42823.0 31 50406.0 32 60609.0 33 72991.0 34 89521.0 35 111197.0 36 133425.0 37 127311.0 38 46124.0 39 1209.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.837989108257474 21.082423589035713 15.756250661212398 22.323336641494414 2 16.451270943008637 22.376010494858978 37.915175881806945 23.257542680325436 3 19.988430516660355 25.38552455817662 30.668522313217284 23.95752261194574 4 13.91907135584804 18.384444531036902 36.78537493368184 30.911109179433225 5 13.38323945547512 38.45771899782805 34.14799599578798 14.01104555090884 6 31.4545440013747 37.802338987317995 16.524068231453047 14.219048779854257 7 27.885820014266503 33.675243219913966 20.659755870946086 17.779180894873452 8 26.3006196138453 35.144053725175986 19.405742167909157 19.149584493069558 9 26.083225097412328 17.132780497552204 20.38675192326906 36.39724248176641 10-11 25.07344305958716 26.97529485871109 27.22785606209329 20.723406019608458 12-13 26.11318832000839 24.51419537086296 27.691900383888186 21.68071592524046 14-15 22.673911816377615 26.083338203728374 26.360276358702844 24.882473621191163 16-17 21.69817352784266 29.33419984694479 27.02743204928138 21.94019457593117 18-19 21.594671440816988 27.81843376166147 29.62132822350057 20.965566574020976 20-21 22.423187664469413 26.540842363124483 29.796012971728747 21.23995700067736 22-23 22.001184889758296 26.65402517840783 29.583929857723312 21.760860074110557 24-25 22.359348056738327 26.8854163960893 29.581715696950685 21.173519850221687 26-27 22.095847328427308 27.05200879967551 29.67168059016171 21.180463281735477 28-29 21.572842091381553 27.546041444793225 29.667384654117207 21.213731809708015 30-31 21.83829097673601 26.957797923164684 29.61283625690097 21.591074843198335 32-33 21.687361498820053 27.283858230741714 29.465308014634918 21.563472255803312 34-35 22.404634452883197 26.86624945800839 29.355014995933814 21.374101093174602 36-37 22.537438094391256 26.658650700006692 29.313311067129312 21.490600138472743 38-39 22.065836751351597 27.15087884405295 29.535923784062167 21.247360620533286 40-41 22.094670033644803 26.989726720268557 29.475945339058196 21.43965790702844 42-43 21.983657449918717 27.425378913154475 28.806442297988756 21.784521338938053 44-45 22.3253936616503 27.15218852998682 28.634070752695262 21.888347055667616 46-47 22.562834252647797 27.607757608696737 28.129024394386406 21.700383744269065 48-49 22.69883298029655 27.69998201510731 27.87708325006994 21.7241017545262 50-51 22.264510228130444 27.80430005579752 28.011305367302054 21.919884348769983 52-53 22.671087941269914 27.958034524306687 27.6104536035919 21.760423930831497 54-55 22.12456448691138 27.705526707306227 27.95901605765609 22.210892748126305 56-57 22.616664626820562 27.850393523780028 27.745786885204954 21.787154964194457 58-59 22.830480670092793 27.44416226412078 27.631886554185282 22.09347051160114 60-61 22.414882674397283 27.545426796826955 28.194912718304156 21.844777810471605 62-63 22.30271137602983 27.583040761559168 28.214626124244923 21.89962173816608 64-65 22.061922013657078 27.795306709675422 27.96698198758602 22.175789289081482 66-67 22.16889329374293 27.765416878139625 27.66865551155142 22.397034316566025 68-69 22.069644186244002 27.66046086448306 27.830749941305466 22.439145007967472 70-71 22.35854537352993 27.619599962235142 27.733793704957538 22.28806095927739 72-73 22.39111800236976 27.314560425837158 27.889916139332193 22.40440543246089 74-75 22.113330789073537 28.028385147650326 27.36271487173134 22.4955691915448 76-77 22.188098350681393 27.59098177342338 27.539679407669976 22.68124046822525 78-79 22.143142978614133 27.67752987661673 27.544839280013626 22.63448786475551 80-81 22.048576154986442 28.000361725631105 27.44348411729703 22.507578002085417 82-83 22.13317132976486 27.80033193412077 27.43637346485465 22.630123271259723 84-85 21.715285163960345 27.768271840605337 27.406324609464182 23.110118385970136 86-87 21.855180086899743 27.785985031102356 27.297180229784217 23.061654652213683 88-89 21.83730514853583 27.612393742367342 27.177369547706753 23.37293156139008 90-91 21.851145848687516 27.579165115634158 27.378201268640495 23.191487767037835 92-93 21.887006219818193 27.81339246598232 27.304457378185027 22.99514393601445 94-95 21.53801191502117 27.648944009392135 27.18663119637304 23.62641287921366 96-97 21.683777721157256 27.583419513096825 27.13068848888156 23.602114276864356 98-99 21.643332327485425 27.77863818851455 26.633170599132306 23.944858884867717 100-101 21.471008483128664 27.657347548485728 26.83486376035411 24.036780208031495 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 227.0 1 165.0 2 124.5 3 150.5 4 147.0 5 168.0 6 255.5 7 461.5 8 720.5 9 884.0 10 875.5 11 833.5 12 883.0 13 1001.0 14 1098.0 15 1181.0 16 1304.0 17 1444.5 18 1594.5 19 1694.5 20 1857.0 21 2123.5 22 2463.0 23 2899.0 24 3470.0 25 4317.0 26 5638.5 27 7225.5 28 8506.5 29 10246.0 30 12455.0 31 14696.0 32 17513.5 33 19828.0 34 21972.5 35 24240.5 36 26595.0 37 28681.0 38 29731.0 39 29935.0 40 30210.0 41 31880.0 42 33186.0 43 34025.5 44 35001.5 45 35797.0 46 35940.0 47 37804.5 48 44058.5 49 43527.5 50 37551.0 51 34527.0 52 32173.5 53 31032.0 54 29729.0 55 27403.5 56 24726.0 57 22232.5 58 20250.0 59 17927.5 60 14995.5 61 12400.0 62 10369.5 63 8206.0 64 6148.0 65 4745.0 66 3591.0 67 2878.5 68 2200.0 69 1506.0 70 1134.0 71 925.5 72 780.0 73 675.0 74 532.0 75 417.0 76 310.0 77 207.0 78 142.0 79 94.0 80 67.5 81 45.5 82 37.5 83 27.0 84 14.0 85 7.5 86 4.5 87 4.5 88 2.0 89 0.5 90 0.5 91 1.5 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.8443012909787341 2 0.007492911705526573 3 0.0036965031080597754 4 0.008891588557224866 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004595652512722964 10-11 4.9952744703510486E-5 12-13 3.4966921292457336E-4 14-15 4.9952744703510486E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.991494046631886E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.00519508544916509 34-35 7.992439152561678E-4 36-37 0.002697448213989566 38-39 4.9952744703510486E-5 40-41 0.004845416236240517 42-43 0.012288375197063578 44-45 0.002297826256361482 46-47 0.0018982042987333982 48-49 0.010589981877144222 50-51 5.494801917386153E-4 52-53 0.002797353703396587 54-55 0.01148913128180741 56-57 0.005344943683275621 58-59 9.990548940702097E-4 60-61 0.007742675429044124 62-63 0.00519508544916509 64-65 0.021929254924841098 66-67 0.0029971646822106288 68-69 9.990548940702097E-5 70-71 0.0015485350858088246 72-73 0.0 74-75 0.0 76-77 0.0017483460646228665 78-79 0.012138516962953046 80-81 0.01873227926381643 82-83 0.014486295964018039 84-85 0.010939651090068794 86-87 0.013737004793465383 88-89 0.03117051269499054 90-91 0.02682462390578513 92-93 0.003246928405728181 94-95 0.010839745600661775 96-97 0.013087619112319747 98-99 0.013187524601726766 100-101 0.01358714655935485 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1000946.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.569505650570676 #Duplication Level Percentage of deduplicated Percentage of total 1 79.9241390969547 47.610414555530404 2 11.87687798701492 14.149995007172475 3 3.6678277398231596 6.554720558181468 4 1.53882410226575 3.6666796422061583 5 0.791612748412789 2.3577990044819708 6 0.4441697705449106 1.587538419377264 7 0.3035812123466981 1.2658927921005612 8 0.21307819596808092 1.0154370238987203 9 0.16113688300207038 0.8638960016256461 >10 0.9504434924269994 10.740979769998859 >50 0.08352687410117515 3.391999706898816 >100 0.04124648469914868 4.3715122041986305 >500 0.002693647573024578 1.0565239026759816 >1k 8.417648665701806E-4 1.3666114116531183 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3625 0.36215739910045097 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3586 0.3582610850135772 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3209 0.32059671550713026 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2119 0.21169973205347742 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1088 0.1086971724748388 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 9.990548940702096E-5 10-11 0.0 0.0 0.0 0.0 9.990548940702096E-5 12-13 0.0 0.0 0.0 0.0 9.990548940702096E-5 14-15 0.0 0.0 0.0 0.0 9.990548940702096E-5 16-17 0.0 0.0 0.0 0.0 9.990548940702096E-5 18-19 0.0 0.0 0.0 0.0 9.990548940702096E-5 20-21 0.0 0.0 0.0 4.995274470351048E-5 9.990548940702096E-5 22-23 0.0 0.0 0.0 1.9981097881404192E-4 9.990548940702096E-5 24-25 0.0 0.0 0.0 2.997164682210629E-4 9.990548940702096E-5 26-27 0.0 0.0 0.0 6.993384258491467E-4 9.990548940702096E-5 28-29 0.0 0.0 0.0 0.0019981097881404193 9.990548940702096E-5 30-31 0.0 0.0 0.0 0.005794518385607216 9.990548940702096E-5 32-33 0.0 0.0 0.0 0.014286484985203997 9.990548940702096E-5 34-35 0.0 0.0 0.0 0.02722424586341321 9.990548940702096E-5 36-37 0.0 0.0 0.0 0.04840420961770166 9.990548940702096E-5 38-39 0.0 0.0 0.0 0.07952476956798868 9.990548940702096E-5 40-41 0.0 0.0 0.0 0.11509112379688814 9.990548940702096E-5 42-43 4.995274470351048E-5 0.0 0.0 0.1610976016688213 9.990548940702096E-5 44-45 9.990548940702096E-5 0.0 0.0 0.21764410867319517 9.990548940702096E-5 46-47 9.990548940702096E-5 0.0 0.0 0.29482109924011884 9.990548940702096E-5 48-49 9.990548940702096E-5 0.0 0.0 0.37839204112909186 9.990548940702096E-5 50-51 9.990548940702096E-5 0.0 0.0 0.4662589190625668 9.990548940702096E-5 52-53 1.4985823411053144E-4 0.0 0.0 0.5598204098922419 9.990548940702096E-5 54-55 1.9981097881404192E-4 0.0 0.0 0.6677682911965281 9.990548940702096E-5 56-57 1.9981097881404192E-4 0.0 0.0 0.803489898555966 9.990548940702096E-5 58-59 1.9981097881404192E-4 0.0 0.0 0.955346242454638 9.990548940702096E-5 60-61 1.9981097881404192E-4 0.0 0.0 1.1234372283819507 9.990548940702096E-5 62-63 1.9981097881404192E-4 0.0 0.0 1.303217156569885 9.990548940702096E-5 64-65 1.9981097881404192E-4 0.0 0.0 1.4932873501667423 9.990548940702096E-5 66-67 1.9981097881404192E-4 0.0 0.0 1.7134790488198164 9.990548940702096E-5 68-69 1.9981097881404192E-4 0.0 0.0 1.9765302024285027 1.4985823411053144E-4 70-71 1.9981097881404192E-4 0.0 0.0 2.290383297400659 1.9981097881404192E-4 72-73 1.9981097881404192E-4 0.0 0.0 2.602537999052896 1.9981097881404192E-4 74-75 1.9981097881404192E-4 0.0 0.0 2.924283627688207 1.9981097881404192E-4 76-77 1.9981097881404192E-4 0.0 0.0 3.313765178141478 1.9981097881404192E-4 78-79 1.9981097881404192E-4 0.0 0.0 3.7291222503511676 1.9981097881404192E-4 80-81 1.9981097881404192E-4 0.0 0.0 4.182243597556711 1.9981097881404192E-4 82-83 1.9981097881404192E-4 0.0 0.0 4.625624159545071 1.9981097881404192E-4 84-85 1.9981097881404192E-4 0.0 0.0 5.114211955490107 1.9981097881404192E-4 86-87 1.9981097881404192E-4 0.0 0.0 5.616786519952125 1.9981097881404192E-4 88-89 1.9981097881404192E-4 0.0 0.0 6.129201775120736 1.9981097881404192E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGACG 60 0.0039517675 31.664934 9 GAGTACT 4205 0.0 23.15222 12-13 GTACATG 4180 0.0 23.151085 1 GTAAGGT 400 0.0 22.56465 4 TACATGG 4275 0.0 22.333227 2 ACATGGG 4055 0.0 22.141443 3 ACCGTTC 130 2.9422287E-4 21.921877 8 GTACTTT 4380 0.0 21.847698 14-15 TAAGGTG 470 0.0 21.222244 5 CATGGGG 1800 0.0 20.849207 4 AGTACTT 4420 0.0 20.30693 12-13 ACGCATT 150 7.7229436E-4 18.99896 5 ACTTTTT 5255 0.0 18.52618 16-17 GTATTAC 210 1.8170378E-5 18.25017 1 GTATCAA 9365 0.0 18.16004 1 CTATAGA 485 0.0 17.779806 1 AGAGTAC 7600 0.0 17.621395 10-11 TACTTTT 5135 0.0 17.479383 14-15 GTGTAAG 360 1.7007551E-9 17.29964 1 GGTAAGG 495 0.0 17.27437 3 >>END_MODULE