FastQCFastQC Report
Thu 26 May 2016
SRR936401_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936401_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1126742
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47910.4252082553060062No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36250.3217240504037304No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23530.20883219051033866No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19420.17235533955421914No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18240.1618826670169391No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT12950.11493314352353955No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC12180.10809928093565341No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG46900.025.9413851
CATGGGG21200.024.4341584
ACATGGG47250.024.2404823
GTATCAA79700.024.1503121
TACATGG49550.023.7866922
GAGTACT40950.023.72948512-13
AGTACTT42550.022.05547512-13
GTACTTT44350.021.6960314-15
TCAACGC90950.020.9531064
ATCAACG91250.020.9372313
CAACGCA91400.020.7970285
GGTATCA59850.020.6459671
TATCAAC93100.020.5722312
AACGCAG93400.020.4534536
TAGCCCT6450.019.1566854
GTATAGT3254.838512E-1019.0101721
GGATCGT1250.00601554119.0084846
AGTACAT37200.018.9079672
ATGGGGA11800.018.5252175
ACGCAGA102850.018.4825737
Produced by FastQC (version 0.11.5)