##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936401_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1126742 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04639926442788 33.0 31.0 34.0 31.0 34.0 2 32.15452517080219 33.0 31.0 34.0 31.0 34.0 3 32.21037469092303 34.0 31.0 34.0 30.0 34.0 4 35.81900825566101 37.0 35.0 37.0 35.0 37.0 5 35.80509380142038 37.0 35.0 37.0 35.0 37.0 6 35.9115431926741 37.0 35.0 37.0 35.0 37.0 7 35.816554277731726 37.0 35.0 37.0 35.0 37.0 8 35.787612425914716 37.0 35.0 37.0 35.0 37.0 9 37.4246828466499 39.0 37.0 39.0 35.0 39.0 10-11 37.34291035569811 39.0 37.0 39.0 35.0 39.0 12-13 37.27501593088746 39.0 37.0 39.0 34.0 39.0 14-15 38.48409085664686 40.0 38.0 41.0 34.0 41.0 16-17 38.378307545116805 40.0 38.0 41.0 34.0 41.0 18-19 38.35716162173772 40.0 38.0 41.0 34.0 41.0 20-21 38.28568962548658 40.0 38.0 41.0 34.0 41.0 22-23 38.14816390975041 40.0 38.0 41.0 34.0 41.0 24-25 38.26627435562001 40.0 38.0 41.0 34.0 41.0 26-27 38.234429887232395 40.0 38.0 41.0 34.0 41.0 28-29 38.14271812003103 40.0 38.0 41.0 34.0 41.0 30-31 37.96620521823097 40.0 38.0 41.0 33.5 41.0 32-33 37.793277875502994 40.0 37.0 41.0 33.0 41.0 34-35 37.58897733465159 40.0 37.0 41.0 33.0 41.0 36-37 37.42504584013021 40.0 37.0 41.0 33.0 41.0 38-39 37.23157963402447 39.0 36.5 41.0 32.0 41.0 40-41 37.051794909571136 39.0 36.0 41.0 31.5 41.0 42-43 36.83657660759961 39.0 36.0 41.0 31.0 41.0 44-45 36.58050600758648 39.0 36.0 40.0 30.5 41.0 46-47 36.32435908131586 39.0 35.5 40.5 30.0 41.0 48-49 36.26711039439375 39.0 36.0 41.0 30.0 41.0 50-51 35.359119035236105 38.0 35.0 40.0 29.0 40.5 52-53 35.50276682683347 38.5 35.0 39.5 29.0 40.5 54-55 35.9259284734216 39.0 35.0 40.5 29.5 41.0 56-57 35.84642624487239 39.0 35.0 41.0 29.0 41.0 58-59 35.610054919404796 39.0 35.0 40.5 28.5 41.0 60-61 35.30737870781421 38.0 35.0 40.0 28.0 41.0 62-63 34.99624492563515 38.0 34.5 40.0 27.5 41.0 64-65 34.67336621870845 37.5 34.0 40.0 26.5 41.0 66-67 34.30526021041197 37.0 34.0 40.0 26.0 41.0 68-69 33.9080055593916 36.0 34.0 39.0 26.0 41.0 70-71 33.47237610739637 36.0 33.0 39.0 25.5 40.5 72-73 33.07008214835339 35.0 33.0 38.5 25.0 40.0 74-75 32.64322311585083 35.0 33.0 37.5 24.0 39.5 76-77 32.20904741280613 35.0 32.0 37.0 22.5 39.0 78-79 31.7649550651347 35.0 32.0 36.5 21.5 39.0 80-81 31.317070811241614 35.0 31.5 36.0 20.0 38.0 82-83 30.85781660752861 34.5 31.0 35.5 18.0 37.0 84-85 30.517598971193053 34.0 31.0 35.0 17.5 37.0 86-87 30.226846962303703 34.0 31.0 35.0 13.5 36.0 88-89 29.838990203613605 34.0 30.0 35.0 7.0 36.0 90-91 29.443116969102064 34.0 30.0 35.0 3.5 36.0 92-93 29.12007495948496 34.0 29.5 35.0 2.0 35.0 94-95 28.706390194028447 34.0 29.0 35.0 2.0 35.0 96-97 28.154199452935984 34.0 29.0 35.0 2.0 35.0 98-99 27.512188238301228 34.0 28.0 35.0 2.0 35.0 100-101 25.140788219485916 31.5 22.0 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3387832867708376 1101 2 0.46396242152845346 1101 3 0.43771791758761225 1101 4 0.4904184207122526 1101 5 0.4099196327783545 1101 6 0.33575325082087204 1101 7 0.2580776766283961 1101 8 0.38459178437822317 1101 9 0.5183811106019931 1101 10-11 0.522421915195828 1101 12-13 0.6184928235703424 1101 14-15 0.7865734997995091 1101 16-17 0.7955470710304766 1101 18-19 0.7137975729306731 1101 20-21 0.6522761744291188 1101 22-23 0.6208048080315294 1101 24-25 0.6479997835231117 1101 26-27 0.4904965595635886 1101 28-29 0.4810372278913917 1101 30-31 0.5485278535087801 1101 32-33 0.4885192102986693 1101 34-35 0.4465149272881348 1101 36-37 0.40096018271577805 1101 38-39 0.41114582884628703 1101 40-41 0.3689701757252237 1101 42-43 0.4220629395486952 1101 44-45 0.455124962712695 1101 46-47 0.40803195325553787 1101 48-49 0.7100653869560247 1101 50-51 0.47861252522992714 1101 52-53 0.4661939636796646 1101 54-55 0.6803797205324926 1101 56-57 0.5763841864138257 1101 58-59 0.526955050270054 1101 60-61 0.41732338237122235 1101 62-63 0.5034020508990977 1101 64-65 0.5646194307216206 1101 66-67 0.5864051116535833 1101 68-69 0.6672342017435113 1101 70-71 0.5147535214613015 1101 72-73 0.47591066015073835 1101 74-75 0.39130362834692534 1101 76-77 0.4284460951210356 1101 78-79 0.3777774677321126 1101 80-81 0.5530986911433367 1101 82-83 0.5292746356962645 1101 84-85 0.6497120183181586 1101 86-87 0.48597352820511475 1101 88-89 0.5667179554878672 1101 90-91 0.42663755716398555 1101 92-93 0.485097399131881 1101 94-95 0.5211549313140402 1101 96-97 0.47108309022715034 1101 98-99 0.3971318416721985 1101 100-101 1.0067058644443172 1102 1 0.33738565082222394 1102 2 0.44523812245370564 1102 3 0.42752437195424875 1102 4 0.4224833030785291 1102 5 0.38190104696852956 1102 6 0.24830665631293414 1102 7 0.3012127784172307 1102 8 0.25571602381968717 1102 9 0.4426780051418149 1102 10-11 0.5004900239623922 1102 12-13 0.4816137048778373 1102 14-15 0.5428281241044317 1102 16-17 0.5358351286665695 1102 18-19 0.5413684791954054 1102 20-21 0.580882280146767 1102 22-23 0.4516357389141916 1102 24-25 0.40354015951067623 1102 26-27 0.3792549174741566 1102 28-29 0.5165578776055639 1102 30-31 0.3832952875317872 1102 32-33 0.34418447226673976 1102 34-35 0.2007303665055531 1102 36-37 0.29136697722552185 1102 38-39 0.2583096627015635 1102 40-41 0.16250328056665353 1102 42-43 0.3227124111323789 1102 44-45 0.14566674585541506 1102 46-47 0.3385350699545313 1102 48-49 0.4201837461317197 1102 50-51 0.45495512244990977 1102 52-53 0.5295889523648398 1102 54-55 0.5914728474892073 1102 56-57 0.5521451134381294 1102 58-59 0.6907545903076553 1102 60-61 0.6063389433732027 1102 62-63 0.6187460915086902 1102 64-65 0.40381022975391545 1102 66-67 0.5574211698043285 1102 68-69 0.4995435283802294 1102 70-71 0.48688340472346425 1102 72-73 0.4157716273860572 1102 74-75 0.39457601259287145 1102 76-77 0.4041896726053942 1102 78-79 0.35059526793020623 1102 80-81 0.561153790825653 1102 82-83 0.6866390317687561 1102 84-85 0.7135745233675692 1102 86-87 0.6150194831445219 1102 88-89 0.6466568820365168 1102 90-91 0.7359116846548552 1102 92-93 0.5956769950595593 1102 94-95 0.7199504126340308 1102 96-97 0.6262739574293228 1102 98-99 0.6298946515054276 1102 100-101 0.8631240657392105 1103 1 0.27370696912960213 1103 2 0.301144793326106 1103 3 0.2591931892818877 1103 4 0.2877100783296811 1103 5 0.42081902553838546 1103 6 0.2842182976018037 1103 7 0.21979410685661094 1103 8 0.29823009870091965 1103 9 0.47198606581913083 1103 10-11 0.3843083552406483 1103 12-13 0.33623934649170195 1103 14-15 0.2634241917403273 1103 16-17 0.37973537623154385 1103 18-19 0.3287574811931293 1103 20-21 0.35239605871471724 1103 22-23 0.5232541829826687 1103 24-25 0.49961257465515274 1103 26-27 0.32309752732513886 1103 28-29 0.11421537767725454 1103 30-31 0.2623618050859591 1103 32-33 0.36358744878152294 1103 34-35 0.32782592276206657 1103 36-37 0.30935625259615307 1103 38-39 0.4466459113753274 1103 40-41 0.3310670213785585 1103 42-43 0.4024358839241202 1103 44-45 0.42114185075988786 1103 46-47 0.5683268859726525 1103 48-49 0.5469132120987084 1103 50-51 0.5657621460307141 1103 52-53 0.5800692666481027 1103 54-55 0.5847971958389309 1103 56-57 0.7135713362083749 1103 58-59 0.59634086048802 1103 60-61 0.49227773078275305 1103 62-63 0.596591320386807 1103 64-65 0.5249375584418203 1103 66-67 0.3800965920507622 1103 68-69 0.4996465689369174 1103 70-71 0.5312026010453579 1103 72-73 0.6224228092937452 1103 74-75 0.47084103137530775 1103 76-77 0.5927296127692223 1103 78-79 0.7466484705033487 1103 80-81 0.5613338728375261 1103 82-83 0.4079016131354294 1103 84-85 0.43590612661363437 1103 86-87 0.5177464256693014 1103 88-89 0.7566311696898111 1103 90-91 0.9045798042124851 1103 92-93 0.7376415521667781 1103 94-95 0.842952066253357 1103 96-97 0.7250143391955426 1103 98-99 0.8538214716814956 1103 100-101 1.2586201037437768 1104 1 0.01928731217916635 1104 2 0.14733020845817535 1104 3 0.1957847919336828 1104 4 0.2707896321547878 1104 5 0.29113945066729485 1104 6 0.14321229949594283 1104 7 0.16831352177821657 1104 8 0.16160377017308747 1104 9 0.34293103056710805 1104 10-11 0.20914998419992514 1104 12-13 0.17922077340951148 1104 14-15 0.3329569708627105 1104 16-17 0.2542026497241281 1104 18-19 0.3249941959148046 1104 20-21 0.3583810108351031 1104 22-23 0.0821119121208369 1104 24-25 0.1368587074553247 1104 26-27 -0.14318969776167734 1104 28-29 0.18332276481815057 1104 30-31 0.22113456105775953 1104 32-33 -0.055922442831651153 1104 34-35 -0.018729901714415575 1104 36-37 0.03743001877525387 1104 38-39 0.13399069723242008 1104 40-41 0.17422023615283422 1104 42-43 0.16785022676322825 1104 44-45 0.25106029756615555 1104 46-47 0.3277891610436825 1104 48-49 0.3568382357754487 1104 50-51 0.24068822200671747 1104 52-53 0.41588164168756236 1104 54-55 0.5156729719096447 1104 56-57 0.5300306122264686 1104 58-59 0.49776830445308207 1104 60-61 0.49741584506245573 1104 62-63 0.7209502070541802 1104 64-65 0.42365217487873963 1104 66-67 0.46041228183293725 1104 68-69 0.40789422757102756 1104 70-71 0.403802306401559 1104 72-73 0.48840670911484807 1104 74-75 0.2843746523371138 1104 76-77 0.4244267084232298 1104 78-79 0.3744010223290921 1104 80-81 0.39363498827475496 1104 82-83 0.4263244795986516 1104 84-85 0.40555965707769914 1104 86-87 0.43839069589975566 1104 88-89 0.5870873414250539 1104 90-91 0.7432631147460036 1104 92-93 0.5244096780217724 1104 94-95 0.6083714075140101 1104 96-97 0.41058047620490257 1104 98-99 0.5412293954950265 1104 100-101 1.1048936489400312 1105 1 0.08991465594629844 1105 2 0.19770338370068075 1105 3 0.17099842653627206 1105 4 0.26201672445809265 1105 5 0.26088064029587343 1105 6 0.13629704426746514 1105 7 0.21344177563972266 1105 8 0.32807794030591 1105 9 0.3602063761471186 1105 10-11 0.31291901759929175 1105 12-13 0.23847513564900424 1105 14-15 0.011368362758851447 1105 16-17 0.23910157076886662 1105 18-19 0.1501761234142691 1105 20-21 0.24008062121237117 1105 22-23 0.10915980720778862 1105 24-25 0.29414238642958423 1105 26-27 0.30946300803295657 1105 28-29 0.33024498281682213 1105 30-31 0.05816599038107029 1105 32-33 0.09684777113189824 1105 34-35 0.1693471310124366 1105 36-37 0.14428843879294107 1105 38-39 0.03365233584089111 1105 40-41 -0.10278014396689628 1105 42-43 0.3781236022996026 1105 44-45 0.03849204048825072 1105 46-47 0.38847491167051373 1105 48-49 0.07098316739044463 1105 50-51 0.23981970323099944 1105 52-53 0.07351894166782103 1105 54-55 0.15468486161643824 1105 56-57 0.4017781213456999 1105 58-59 0.2931496668899456 1105 60-61 0.0886432114905702 1105 62-63 0.4045366185045509 1105 64-65 0.47241623748153216 1105 66-67 0.4139700563780764 1105 68-69 0.5485517798923212 1105 70-71 0.4855892712185508 1105 72-73 0.5162587036469759 1105 74-75 0.4412599365862633 1105 76-77 0.04703588319789631 1105 78-79 0.30944029527699257 1105 80-81 0.05490489887351657 1105 82-83 0.29177808306066666 1105 84-85 0.5059373096921185 1105 86-87 0.721637923584165 1105 88-89 0.5501015081952794 1105 90-91 0.5533279726762466 1105 92-93 0.4731707252516486 1105 94-95 0.44257523941336885 1105 96-97 0.6612520819914955 1105 98-99 0.5970306988671297 1105 100-101 1.1782316302118225 1106 1 0.06237258213479535 1106 2 0.2507973359115958 1106 3 0.19126112535975892 1106 4 0.2909704079751947 1106 5 0.3512863996634721 1106 6 0.1121262778331058 1106 7 0.2874005392090382 1106 8 0.17370186308214386 1106 9 0.47463477006639465 1106 10-11 0.3155165537304043 1106 12-13 0.4065582045233569 1106 14-15 0.41777907495951894 1106 16-17 0.6114280081273833 1106 18-19 0.4366034960949179 1106 20-21 0.3322134240592902 1106 22-23 0.28806565284042307 1106 24-25 0.07871244671054711 1106 26-27 0.053585222064363336 1106 28-29 0.3610664351018045 1106 30-31 0.5832734731324152 1106 32-33 0.302814261957316 1106 34-35 0.22046308690747196 1106 36-37 0.15258948808948958 1106 38-39 0.13813759497583789 1106 40-41 0.14256372241465698 1106 42-43 -0.0321420243069781 1106 44-45 0.14491521319434497 1106 46-47 0.2615681493629296 1106 48-49 0.33240805668364004 1106 50-51 0.11694299949650855 1106 52-53 0.322742556329743 1106 54-55 0.5944457244759391 1106 56-57 0.40909452930438306 1106 58-59 0.2354475060814636 1106 60-61 0.24673522055972796 1106 62-63 0.5262064830771607 1106 64-65 0.2785769553460682 1106 66-67 0.35414089600701715 1106 68-69 0.14903268569263872 1106 70-71 0.5703268076633563 1106 72-73 0.7000385239020019 1106 74-75 0.3791190474812751 1106 76-77 0.5798388172548137 1106 78-79 0.5813337518560537 1106 80-81 0.5349306215392637 1106 82-83 0.6132369032686427 1106 84-85 0.6432995688253165 1106 86-87 0.7165269379494745 1106 88-89 0.7629129990468257 1106 90-91 0.8563770496403471 1106 92-93 0.853416118785777 1106 94-95 0.8764587519862808 1106 96-97 0.795565002751399 1106 98-99 1.2917056190937544 1106 100-101 1.7948183927982448 1107 1 0.09305912859804266 1107 2 0.20026676769725427 1107 3 0.16183082573181906 1107 4 0.21557908639802292 1107 5 0.2510907388748862 1107 6 0.17034430614475582 1107 7 0.1463816721152753 1107 8 0.27671135166722394 1107 9 0.18129907009060986 1107 10-11 0.24037978381208092 1107 12-13 0.2876708842230684 1107 14-15 0.217107746557609 1107 16-17 0.3192969566776753 1107 18-19 0.3957128679990163 1107 20-21 0.4565577815433812 1107 22-23 0.29789822664664456 1107 24-25 0.35905431646815344 1107 26-27 0.44767453254583955 1107 28-29 0.38016400131274963 1107 30-31 0.46861239834651514 1107 32-33 0.3042418151792461 1107 34-35 0.46489046951099766 1107 36-37 0.3337552796488339 1107 38-39 0.2583183128940689 1107 40-41 0.04745703087072428 1107 42-43 0.4122893485657997 1107 44-45 0.5181209679881746 1107 46-47 0.6444475551705651 1107 48-49 0.7304270943958144 1107 50-51 0.6297909784939364 1107 52-53 0.7618709989689378 1107 54-55 0.6611705940354184 1107 56-57 0.8143366423308223 1107 58-59 0.7592074955141257 1107 60-61 0.6209873890646733 1107 62-63 0.7461487480090199 1107 64-65 0.9115636856368852 1107 66-67 0.8856275602519972 1107 68-69 0.8182737640009847 1107 70-71 0.911371680304967 1107 72-73 0.7094624390991555 1107 74-75 0.8749416483662884 1107 76-77 0.8787767176576899 1107 78-79 0.8490120205745448 1107 80-81 0.9672480873605487 1107 82-83 0.894257806775677 1107 84-85 0.9002873558495104 1107 86-87 0.8857542567086973 1107 88-89 1.0215457401691665 1107 90-91 0.7804780007003842 1107 92-93 0.8773508544923594 1107 94-95 0.7224199115310022 1107 96-97 0.9133075285643102 1107 98-99 0.8674368015343674 1107 100-101 1.3372211953432078 1108 1 -0.0775134806109321 1108 2 -0.15265109366216834 1108 3 -0.20644586510981 1108 4 -0.0363183667981275 1108 5 0.12235275607845608 1108 6 0.05206761530313031 1108 7 -0.11098044628580794 1108 8 -0.023980164608708776 1108 9 -0.08574216789218525 1108 10-11 0.016457115571228087 1108 12-13 0.00267025173867097 1108 14-15 0.13811571586102644 1108 16-17 -0.033430105221462725 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-4.2168785826433 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2009.0 3 659.0 4 606.0 5 584.0 6 559.0 7 682.0 8 837.0 9 1033.0 10 1303.0 11 1782.0 12 2621.0 13 3355.0 14 4132.0 15 5545.0 16 7237.0 17 10188.0 18 8410.0 19 4934.0 20 4719.0 21 4920.0 22 5236.0 23 5881.0 24 6670.0 25 7808.0 26 8972.0 27 10991.0 28 13442.0 29 17112.0 30 21874.0 31 28921.0 32 38629.0 33 53697.0 34 76855.0 35 114698.0 36 172498.0 37 241476.0 38 202793.0 39 33056.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.62131660996726 18.690030610578052 14.484272128749085 23.204380650705605 2 15.183410133339962 19.82514276279406 40.820438891842926 24.171008212023054 3 19.070254206145044 24.474997470188164 30.37442990468379 26.080318418983 4 12.317895378025531 16.376563100744356 38.052152046648615 33.253389474581496 5 13.39054765023198 37.10648877838702 34.87548560392479 14.627477967456212 6 31.30420189854816 36.177633500824435 17.81831226153828 14.69985233908912 7 27.89773388184504 32.87678287762642 21.26338240399787 17.962100836530666 8 26.05934366640119 33.97446654360745 20.375357257963557 19.590832532027804 9 26.081733650473176 16.789944571554898 21.067159600716316 36.0611621772556 10-11 24.612816511541695 26.100684355278723 27.939187164398433 21.347311968781145 12-13 25.85006714239737 23.81621849578834 28.14391778663145 22.18979657518284 14-15 22.685512555714023 25.078136131985147 26.87478998348277 25.361561328818063 16-17 21.7563834448384 28.510434095113208 27.41191203477839 22.321270425270004 18-19 21.49460766926032 26.956592769708653 29.999764711209647 21.549034849821382 20-21 22.32180064856651 26.008386896062934 29.831884850375694 21.83792760499486 22-23 21.771386395984685 26.08734438056909 29.78630580075298 22.354963422693242 24-25 21.983984166113796 26.144018248021318 29.890454181357462 21.98154340450742 26-27 21.68824840433605 26.369439584892163 29.86574321859464 22.07656879217714 28-29 21.34751880059608 26.607292314765424 30.112106847206828 21.93308203743167 30-31 21.560889060880186 26.295763096979424 29.9583197239303 22.18502811821009 32-33 21.384216605966312 26.47443728840486 29.865151576338 22.276194529290823 34-35 22.136038662779086 26.400882249825813 29.49958505855778 21.96349402883732 36-37 22.070504405526812 26.07435474809014 29.640980852134597 22.21415999424845 38-39 21.667035016291706 26.466630630534677 29.703846901469582 22.162487451704038 40-41 21.803615075892452 26.3454912729448 29.887079911176272 21.963813739986474 42-43 21.695078018426923 26.58861421771672 29.183932845842047 22.53237491801431 44-45 21.844954456580876 26.557187322217363 29.19567029102879 22.40218793017297 46-47 22.009543474373157 26.78860267932621 28.788933723308467 22.41292012299216 48-49 22.096583662677848 26.870696074402723 28.5089280246518 22.523792238267628 50-51 21.90920963471116 27.022552260351446 28.629668729700814 22.43856937523658 52-53 22.188130982546607 27.140093102514832 28.247560385723098 22.424215529215463 54-55 21.70327902535876 26.77254598974076 28.62540455839065 22.898770426509827 56-57 22.030107122204168 27.174831052968173 28.255151181353273 22.539910643474386 58-59 22.220318854523732 26.783948633924403 28.306238450354943 22.68949406119692 60-61 22.113947419807218 26.755187900520124 28.770924680027694 22.35993999964497 62-63 21.894150220045166 26.908526267628353 28.71530633029807 22.48201718202841 64-65 21.776680020803344 26.910996483667066 28.632242341192573 22.680081154337017 66-67 21.720549783526728 27.029514563796152 28.303414047064962 22.94652160561215 68-69 21.623214571819005 26.8583254444514 28.6394596770507 22.879000306678897 70-71 21.870358330763104 26.8091942494891 28.401124540409565 22.91932287933823 72-73 21.850792737910126 26.6877187750726 28.449412813030296 23.012075673986978 74-75 21.674564242840038 27.186862362066073 28.205307987720076 22.933265407373813 76-77 21.811852689448035 26.77323927535833 28.28799811126663 23.126909923927002 78-79 21.787468019908093 26.88085099773449 28.357449225710106 22.97423175664731 80-81 21.85219522738257 27.083931548901596 28.163670891862303 22.90020233185353 82-83 21.917691499332555 27.014444105200376 28.15482192053168 22.913042474935384 84-85 21.713389472301394 26.945195715638697 27.97727516822607 23.36413964383384 86-87 21.792512404550088 26.97213597011104 28.16646083020067 23.068890795138206 88-89 21.89342075074898 26.752116687516615 27.93335235641383 23.421110205320574 90-91 21.682756270241438 26.882536870590595 28.201908031429536 23.232798827738435 92-93 21.954933535597323 26.886316601432387 28.170556831047584 22.98819303192271 94-95 21.745007724120423 26.72869631669737 28.081005409325126 23.445290549857077 96-97 21.82812753791957 26.70727929696688 28.019874656174302 23.444718508939246 98-99 21.79146277864696 26.8509037648547 27.589183671204907 23.768449785293434 100-101 21.969340831880636 26.57922504653299 27.768951344229574 23.682482777356803 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 146.0 1 125.5 2 140.0 3 192.5 4 170.0 5 162.5 6 306.5 7 636.0 8 1094.0 9 1310.5 10 1157.0 11 1012.0 12 1021.5 13 1097.0 14 1196.0 15 1286.5 16 1342.5 17 1402.0 18 1491.0 19 1612.5 20 1844.5 21 2169.0 22 2567.5 23 3046.5 24 3591.0 25 4522.0 26 5993.5 27 7685.0 28 9139.5 29 10859.5 30 13218.5 31 15941.0 32 19161.5 33 21729.0 34 24080.0 35 26525.0 36 29334.0 37 32097.0 38 33222.0 39 32941.0 40 33298.5 41 35234.0 42 36674.5 43 37982.5 44 39235.5 45 40571.5 46 40605.0 47 42126.0 48 49794.0 49 49695.5 50 42388.0 51 38809.0 52 36445.5 53 35853.5 54 34923.0 55 32212.5 56 29184.0 57 25952.0 58 23458.5 59 20829.5 60 17526.5 61 14577.0 62 11998.0 63 9524.5 64 7269.0 65 5666.0 66 4322.5 67 3491.0 68 2611.5 69 1712.0 70 1331.5 71 1107.5 72 920.5 73 758.0 74 607.0 75 508.5 76 366.5 77 212.5 78 123.5 79 96.0 80 72.0 81 47.0 82 35.5 83 23.5 84 14.0 85 12.0 86 8.5 87 7.0 88 6.5 89 4.0 90 2.5 91 1.5 92 3.0 93 3.0 94 0.5 95 0.5 96 0.5 97 0.5 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029642988368233366 2 0.01979157606621569 3 0.015620257343739737 4 0.047482032266481594 5 0.050322079056252446 6 0.04552950009851412 7 0.048192043963924305 8 0.040381915292054434 9 0.037719371426644256 10-11 0.04433135535907954 12-13 0.0358999664519473 14-15 0.03039737579676625 16-17 0.036432475225029334 18-19 0.04140255710712834 20-21 0.009185776335665129 22-23 0.013800852369042781 24-25 0.0039494400670251045 26-27 0.008431388907132246 28-29 0.004348821646836632 30-31 0.006212602352623759 32-33 0.002884422520861031 34-35 0.006833862587886136 36-37 0.007898880134050209 38-39 0.008431388907132246 40-41 0.0014200233948854306 42-43 0.001863780705787128 44-45 0.0013312719327050913 46-47 3.10630117631188E-4 48-49 7.987631596230549E-4 50-51 3.10630117631188E-4 52-53 0.002396289478869164 54-55 0.004481948840107141 56-57 0.011670817276714634 58-59 0.007455122823148512 60-61 0.007277619898787832 62-63 0.003683185680484086 64-65 0.0 66-67 0.003905064335934934 68-69 0.013889603831223118 70-71 0.005280711999730196 72-73 0.001242520470524752 74-75 0.0022187865545084855 76-77 0.005413839193000705 78-79 0.005724469310631892 80-81 0.011315811427993275 82-83 0.004792578957738328 84-85 0.011803944469985143 86-87 0.003416931293943068 88-89 0.0035944342183037467 90-91 0.002751295327590522 92-93 0.014333361142124818 94-95 0.006789486856795966 96-97 0.007188868436607493 98-99 0.01814967401587941 100-101 0.03465744598142254 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1126742.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.12087332031498 #Duplication Level Percentage of deduplicated Percentage of total 1 77.94949212150658 44.52543064855469 2 12.924297545413687 14.764943256912662 3 4.078310401820173 6.98869955469879 4 1.7401239406812037 3.9758959670919336 5 0.8845558127492378 2.5263300262398736 6 0.5218541836280435 1.7885260028816317 7 0.3297818111246374 1.318619753961612 8 0.22590560155449363 1.0323140198995024 9 0.17545457324564667 0.9019906606651059 >10 1.0270102333290667 11.259948566897638 >50 0.09254337542647657 3.6218864105620114 >100 0.047717218276859076 5.020520342809022 >500 0.00170973650958891 0.6683607684610927 >1k 0.0012434447342464803 1.6065340203644358 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4791 0.4252082553060062 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3625 0.3217240504037304 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2353 0.20883219051033866 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1942 0.17235533955421914 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1824 0.1618826670169391 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1295 0.11493314352353955 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1218 0.10809928093565341 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 8.875146218033941E-5 6 0.0 0.0 0.0 0.0 8.875146218033941E-5 7 0.0 0.0 0.0 0.0 8.875146218033941E-5 8 0.0 0.0 0.0 0.0 8.875146218033941E-5 9 0.0 0.0 0.0 0.0 8.875146218033941E-5 10-11 0.0 0.0 0.0 0.0 8.875146218033941E-5 12-13 0.0 0.0 0.0 0.0 8.875146218033941E-5 14-15 0.0 0.0 0.0 8.875146218033941E-5 8.875146218033941E-5 16-17 0.0 0.0 0.0 8.875146218033941E-5 8.875146218033941E-5 18-19 0.0 0.0 0.0 8.875146218033941E-5 8.875146218033941E-5 20-21 8.875146218033941E-5 0.0 0.0 1.331271932705091E-4 8.875146218033941E-5 22-23 8.875146218033941E-5 0.0 0.0 2.662543865410182E-4 8.875146218033941E-5 24-25 8.875146218033941E-5 0.0 0.0 3.993815798115274E-4 8.875146218033941E-5 26-27 8.875146218033941E-5 0.0 0.0 8.431388907132245E-4 8.875146218033941E-5 28-29 8.875146218033941E-5 0.0 0.0 0.002440665209959334 8.875146218033941E-5 30-31 1.7750292436067883E-4 0.0 0.0 0.005058833344279347 8.875146218033941E-5 32-33 1.7750292436067883E-4 0.0 0.0 0.011892695932165482 8.875146218033941E-5 34-35 1.7750292436067883E-4 0.0 0.0 0.024317900637413 8.875146218033941E-5 36-37 1.7750292436067883E-4 0.0 0.0 0.04362134366163683 8.875146218033941E-5 38-39 1.7750292436067883E-4 0.0 0.0 0.07428497384494409 8.875146218033941E-5 40-41 1.7750292436067883E-4 0.0 0.0 0.11129433357414564 8.875146218033941E-5 42-43 1.7750292436067883E-4 0.0 0.0 0.15558131320213503 8.875146218033941E-5 44-45 1.7750292436067883E-4 0.0 0.0 0.21074034694721594 8.875146218033941E-5 46-47 1.7750292436067883E-4 0.0 0.0 0.2859572111450536 8.875146218033941E-5 48-49 1.7750292436067883E-4 0.0 0.0 0.36601103003171975 8.875146218033941E-5 50-51 1.7750292436067883E-4 0.0 0.0 0.4507686764139439 8.875146218033941E-5 52-53 1.7750292436067883E-4 0.0 0.0 0.5353044441407173 8.875146218033941E-5 54-55 1.7750292436067883E-4 0.0 0.0 0.6308453931778526 8.875146218033941E-5 56-57 1.7750292436067883E-4 0.0 0.0 0.7575381054402872 8.875146218033941E-5 58-59 1.7750292436067883E-4 0.0 0.0 0.896079137903797 8.875146218033941E-5 60-61 1.7750292436067883E-4 0.0 0.0 1.057429296147654 8.875146218033941E-5 62-63 1.7750292436067883E-4 0.0 0.0 1.2269445889121022 8.875146218033941E-5 64-65 1.7750292436067883E-4 0.0 0.0 1.408041947491085 8.875146218033941E-5 66-67 1.7750292436067883E-4 0.0 0.0 1.62113420818608 8.875146218033941E-5 68-69 1.7750292436067883E-4 0.0 0.0 1.8738096210135062 8.875146218033941E-5 70-71 1.7750292436067883E-4 0.0 0.0 2.167976342410241 8.875146218033941E-5 72-73 1.7750292436067883E-4 0.0 0.0 2.4593473927482954 8.875146218033941E-5 74-75 1.7750292436067883E-4 0.0 0.0 2.764652422648663 8.875146218033941E-5 76-77 1.7750292436067883E-4 0.0 0.0 3.1214776763447176 8.875146218033941E-5 78-79 1.7750292436067883E-4 0.0 0.0 3.5142028964927197 8.875146218033941E-5 80-81 1.7750292436067883E-4 0.0 0.0 3.9331097979839216 8.875146218033941E-5 82-83 1.7750292436067883E-4 0.0 0.0 4.354324237491813 8.875146218033941E-5 84-85 1.7750292436067883E-4 0.0 0.0 4.82177818879566 8.875146218033941E-5 86-87 1.7750292436067883E-4 0.0 0.0 5.290208406183492 8.875146218033941E-5 88-89 1.7750292436067883E-4 0.0 0.0 5.790722277149516 8.875146218033941E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 4690 0.0 25.941385 1 CATGGGG 2120 0.0 24.434158 4 ACATGGG 4725 0.0 24.240482 3 GTATCAA 7970 0.0 24.150312 1 TACATGG 4955 0.0 23.786692 2 GAGTACT 4095 0.0 23.729485 12-13 AGTACTT 4255 0.0 22.055475 12-13 GTACTTT 4435 0.0 21.69603 14-15 TCAACGC 9095 0.0 20.953106 4 ATCAACG 9125 0.0 20.937231 3 CAACGCA 9140 0.0 20.797028 5 GGTATCA 5985 0.0 20.645967 1 TATCAAC 9310 0.0 20.572231 2 AACGCAG 9340 0.0 20.453453 6 TAGCCCT 645 0.0 19.156685 4 GTATAGT 325 4.838512E-10 19.010172 1 GGATCGT 125 0.006015541 19.008484 6 AGTACAT 3720 0.0 18.907967 2 ATGGGGA 1180 0.0 18.525217 5 ACGCAGA 10285 0.0 18.482573 7 >>END_MODULE