FastQCFastQC Report
Thu 26 May 2016
SRR936401_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936401_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1126742
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39900.3541183340995543No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38710.34355691010009387No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37230.33042169369740365No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22880.2030633454686166No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA11450.10162042419648865No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG700.0083930727.1418175
GAGTACT49050.022.70910812-13
GTACATG48200.021.7888491
GTACTTT51900.021.55359814-15
ACATGGG49000.020.2603283
TACATGG52050.020.0778622
GTATCAA103850.019.4936051
CATGGGG21000.019.2254524
AGTACTT51800.019.02762412-13
GTATTAG4500.019.0085531
GATCCGT1250.006033953318.9984286
TAAGGTG5850.018.6744985
ACTTTTT60250.018.60550916-17
GCGTGCG2352.5271802E-618.1915999
GGTATCA82750.017.4580041
GTGTAAG4103.274181E-1117.385871
TATCAAC117150.017.233052
TCAACGC117250.017.1763154
ATCAACG117550.017.133243
GTACACG1952.267456E-417.0589561
Produced by FastQC (version 0.11.5)