##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936401_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1126742 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.359194030221648 31.0 31.0 34.0 30.0 34.0 2 31.525852413418512 31.0 31.0 34.0 30.0 34.0 3 31.606539917745145 31.0 31.0 34.0 30.0 34.0 4 35.44505751982264 37.0 35.0 37.0 33.0 37.0 5 35.15060590623231 37.0 35.0 37.0 32.0 37.0 6 35.29593820058185 37.0 35.0 37.0 32.0 37.0 7 35.222176860363774 37.0 35.0 37.0 32.0 37.0 8 35.18718837142842 37.0 35.0 37.0 32.0 37.0 9 36.720942327524845 39.0 35.0 39.0 32.0 39.0 10-11 36.551063153765455 38.0 35.0 39.0 32.0 39.0 12-13 36.43364851935936 38.0 35.0 39.0 32.0 39.0 14-15 37.36355305828664 39.0 36.0 40.0 32.0 41.0 16-17 37.2503949440067 39.0 36.0 40.0 32.0 41.0 18-19 37.421737629377446 39.0 36.0 40.0 32.0 41.0 20-21 37.43215394473624 39.0 36.0 40.0 32.0 41.0 22-23 37.325925544623345 39.0 36.0 40.0 32.0 41.0 24-25 37.14315344595302 39.0 36.0 40.0 31.0 41.0 26-27 36.85915497957829 39.0 36.0 40.0 31.0 41.0 28-29 36.73195017137907 39.0 35.5 40.0 30.0 41.0 30-31 36.69202621363187 39.0 35.5 40.0 30.0 41.0 32-33 36.52794694792597 38.0 35.0 40.0 30.0 41.0 34-35 36.30795337353183 38.0 35.0 40.0 30.0 41.0 36-37 36.028191458204276 38.0 35.0 40.0 29.5 41.0 38-39 35.79621421762924 38.0 35.0 40.0 29.0 41.0 40-41 35.501325503087656 38.0 34.0 40.0 27.5 41.0 42-43 35.21530084083135 38.0 34.0 40.0 27.0 41.0 44-45 34.90495117782065 38.0 33.5 40.0 26.0 41.0 46-47 34.48873655193469 38.0 33.0 40.0 24.5 41.0 48-49 34.07860406375195 38.0 33.0 40.0 24.0 41.0 50-51 33.68381270956439 37.0 32.5 40.0 23.0 41.0 52-53 33.33510555211397 37.0 32.0 40.0 22.0 41.0 54-55 33.015582537972314 37.0 31.5 39.0 20.5 40.0 56-57 32.63240741891222 36.0 31.0 39.0 19.0 40.0 58-59 32.201315385420976 36.0 31.0 39.0 18.0 40.0 60-61 31.779306176569257 35.0 30.0 39.0 15.0 40.0 62-63 31.307290843866653 35.0 29.0 38.0 13.0 40.0 64-65 30.817375228756895 34.5 29.0 38.0 9.0 40.0 66-67 30.260063972053942 34.0 28.0 37.5 8.0 39.5 68-69 29.8770943126288 34.0 28.0 37.0 7.0 39.0 70-71 29.346915709186305 34.0 27.5 36.5 4.5 39.0 72-73 28.79982906468384 33.0 26.0 36.0 2.0 39.0 74-75 28.169816603978553 32.5 26.0 35.5 2.0 38.0 76-77 27.024613886763785 31.0 25.5 34.0 2.0 36.0 78-79 27.795332915609784 32.0 26.0 35.0 2.0 37.0 80-81 27.542822136744704 32.0 26.0 35.0 2.0 36.5 82-83 27.090482559450166 32.0 25.5 35.0 2.0 36.0 84-85 26.514222421814402 32.0 24.5 34.0 2.0 36.0 86-87 25.999495004180197 31.5 23.5 34.0 2.0 35.0 88-89 25.409133590475903 31.0 21.0 34.0 2.0 35.0 90-91 24.703264811287767 31.0 19.0 34.0 2.0 35.0 92-93 23.977437603284514 30.5 15.0 34.0 2.0 35.0 94-95 23.089028810499652 30.0 4.5 34.0 2.0 35.0 96-97 21.980155616813786 29.0 2.0 34.0 2.0 35.0 98-99 20.61806828892506 29.0 2.0 34.0 2.0 34.5 100-101 17.457913612876773 22.0 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.402148988445866 1101 2 0.416253229624477 1101 3 0.35132048604979715 1101 4 0.27793319914495385 1101 5 0.3194732221419514 1101 6 0.26771959881197915 1101 7 0.2993475913702852 1101 8 0.31359557281230366 1101 9 0.4188453430587913 1101 10-11 0.37979220497060595 1101 12-13 0.32384732622641366 1101 14-15 0.5723855597130623 1101 16-17 0.5406994895648296 1101 18-19 0.3938159510678858 1101 20-21 0.3310214492307466 1101 22-23 0.29783776431271036 1101 24-25 0.350500682682906 1101 26-27 0.31796156870208847 1101 28-29 0.3171888637924525 1101 30-31 0.48464950449638877 1101 32-33 0.618612188614442 1101 34-35 0.4704787639181234 1101 36-37 0.45150507045881483 1101 38-39 0.606007573292878 1101 40-41 0.6390293603421071 1101 42-43 0.7885590961042084 1101 44-45 0.8022771712744543 1101 46-47 0.879827674495921 1101 48-49 0.8627855417411254 1101 50-51 0.7521958386317564 1101 52-53 0.8874958004240838 1101 54-55 1.118161469290449 1101 56-57 1.0429882554506804 1101 58-59 0.9701855070624745 1101 60-61 0.8927066417999256 1101 62-63 0.8712241416480033 1101 64-65 1.0107846658450903 1101 66-67 0.9790526525517684 1101 68-69 1.1055523029153527 1101 70-71 0.9043882480382344 1101 72-73 0.8029377092546746 1101 74-75 0.7683924333965919 1101 76-77 0.6066206312167814 1101 78-79 0.7342052059683688 1101 80-81 0.95781611590035 1101 82-83 1.1582741122812585 1101 84-85 1.0398169816552532 1101 86-87 1.0569706577269784 1101 88-89 1.026145494817463 1101 90-91 1.1867215406175369 1101 92-93 1.032483369389496 1101 94-95 1.1129210470203041 1101 96-97 1.0784520672343412 1101 98-99 1.2674877463811036 1101 100-101 1.3805973331281258 1102 1 0.36231966492899303 1102 2 0.4310206053038925 1102 3 0.3779366763027028 1102 4 0.25950875539346896 1102 5 0.1772665663978117 1102 6 0.321519140384936 1102 7 0.339533271367074 1102 8 0.31827334656232154 1102 9 0.3866201847212096 1102 10-11 0.4624059546192001 1102 12-13 0.3543508114112086 1102 14-15 0.5776390157644471 1102 16-17 0.5495031528050376 1102 18-19 0.5406030388954051 1102 20-21 0.4307440715415396 1102 22-23 0.4210630253657115 1102 24-25 0.28894075330321556 1102 26-27 0.19538261639628018 1102 28-29 0.2536493211969528 1102 30-31 0.5002149590136469 1102 32-33 0.6102487891425099 1102 34-35 0.5793779110423856 1102 36-37 0.5923410357787802 1102 38-39 0.7231099820999347 1102 40-41 0.7489403080707078 1102 42-43 0.7816762418868635 1102 44-45 0.8623846524421452 1102 46-47 0.7538021516337565 1102 48-49 0.7177768585266833 1102 50-51 0.7342342284040626 1102 52-53 0.7238815012710376 1102 54-55 0.9981965399268802 1102 56-57 0.9091446153390876 1102 58-59 0.6963832049167067 1102 60-61 0.934741659927127 1102 62-63 0.7824001011428159 1102 64-65 0.8496804459303071 1102 66-67 0.8714739896608528 1102 68-69 0.9450080278873756 1102 70-71 0.9645032524577601 1102 72-73 0.8589975273977188 1102 74-75 0.5219336971278707 1102 76-77 0.33203268662905927 1102 78-79 0.2832188078541549 1102 80-81 0.8387667202247471 1102 82-83 0.7395200119170866 1102 84-85 0.6426041008040642 1102 86-87 0.6987276840219714 1102 88-89 0.7091778443397594 1102 90-91 0.9781735451112219 1102 92-93 1.0752504459812648 1102 94-95 1.3014611845668433 1102 96-97 1.7143842541776344 1102 98-99 1.5311828667538983 1102 100-101 1.2489150157946014 1103 1 0.1336571848823489 1103 2 0.1538982852664219 1103 3 0.22676470945155458 1103 4 0.10542718321250533 1103 5 0.002660232940222329 1103 6 0.1926477116769547 1103 7 0.1344233009028244 1103 8 0.1266051027442785 1103 9 0.10011524775947578 1103 10-11 0.08854097789413373 1103 12-13 0.15266698724925476 1103 14-15 0.17014467917166343 1103 16-17 0.39859022111959064 1103 18-19 0.112115766029099 1103 20-21 0.16048570547037144 1103 22-23 0.17023162762725264 1103 24-25 0.12395838872316034 1103 26-27 0.2525181271543673 1103 28-29 0.3464495773645737 1103 30-31 0.18888489468498904 1103 32-33 0.24902874307057488 1103 34-35 0.2634734386234143 1103 36-37 0.16297877844502295 1103 38-39 0.2843265871143288 1103 40-41 0.4022591124943773 1103 42-43 0.21676699977979297 1103 44-45 0.3423758586284009 1103 46-47 0.47503013125505333 1103 48-49 0.6538743169233427 1103 50-51 0.5563576476682499 1103 52-53 0.3748313287402354 1103 54-55 0.48404318346199204 1103 56-57 0.45669467794721896 1103 58-59 0.5871739790590667 1103 60-61 0.696436954280923 1103 62-63 0.6263577952519164 1103 64-65 0.4469514961219545 1103 66-67 0.4774030274073411 1103 68-69 0.6869363583053669 1103 70-71 0.5520024638820722 1103 72-73 0.3849866880664301 1103 74-75 0.39158787084392443 1103 76-77 0.3568795117755421 1103 78-79 0.4029953302831579 1103 80-81 0.6417894981140364 1103 82-83 0.5247109428187393 1103 84-85 0.2898774450340831 1103 86-87 -0.033267460263484594 1103 88-89 0.07642748350664164 1103 90-91 -0.020188224739378313 1103 92-93 0.05634833451011545 1103 94-95 0.42130260916466966 1103 96-97 0.6029898611897444 1103 98-99 0.8878937892440781 1103 100-101 1.0779259985032859 1104 1 0.1230978878182647 1104 2 0.29507686045320725 1104 3 0.22350468630097353 1104 4 0.13952904578221847 1104 5 0.059700641232339535 1104 6 0.21931560602610745 1104 7 0.2508523233167921 1104 8 0.1600608535598127 1104 9 -0.028770893459089564 1104 10-11 0.2223931824611114 1104 12-13 0.21658969586758303 1104 14-15 0.2958668195483867 1104 16-17 0.3741842440597125 1104 18-19 0.23578539667204979 1104 20-21 0.11942446165262766 1104 22-23 0.09262560847540158 1104 24-25 0.17885342187059194 1104 26-27 0.3016255142952744 1104 28-29 0.2360657512044142 1104 30-31 0.015648022118433857 1104 32-33 0.16800932814896896 1104 34-35 -0.010630002395217275 1104 36-37 0.09374669384015988 1104 38-39 0.25788993553060635 1104 40-41 0.2673377718651011 1104 42-43 0.22702086697962187 1104 44-45 0.3145004508624325 1104 46-47 0.1767046024746648 1104 48-49 0.300450661235665 1104 50-51 0.08996440342958323 1104 52-53 0.17342624613473845 1104 54-55 0.4072405948057778 1104 56-57 0.43999679306875805 1104 58-59 0.11786244585902494 1104 60-61 0.29665136025798233 1104 62-63 0.41398459731442117 1104 64-65 0.5283021222400208 1104 66-67 0.2008424675841276 1104 68-69 0.42992146827064204 1104 70-71 0.12794979630341174 1104 72-73 0.09374102880093815 1104 74-75 0.24702694271596215 1104 76-77 0.17460549304461637 1104 78-79 0.3323939412481174 1104 80-81 0.3368037041542351 1104 82-83 0.3139033031260112 1104 84-85 0.4311007429400071 1104 86-87 0.21619455173272684 1104 88-89 0.48786545046444374 1104 90-91 0.7265701673473295 1104 92-93 0.9669873432441989 1104 94-95 0.9948157102906308 1104 96-97 1.2943849469521318 1104 98-99 1.7264574007351854 1104 100-101 1.8496586092577836 1105 1 0.0032112292900592365 1105 2 0.09067478149064101 1105 3 -0.002063483056989668 1105 4 -0.0555984770797906 1105 5 -0.1906365322850121 1105 6 -0.043885331115248505 1105 7 0.09154468948906214 1105 8 -0.018019761064564932 1105 9 -0.018024287124248417 1105 10-11 0.12306177287478448 1105 12-13 -0.4205088026594552 1105 14-15 -0.09258402804552901 1105 16-17 -0.016962621048946858 1105 18-19 -0.1383771520307917 1105 20-21 -0.15411098085791508 1105 22-23 -0.14435529050777518 1105 24-25 -0.15934852039506353 1105 26-27 0.10848311193852567 1105 28-29 -0.07106779874843738 1105 30-31 -0.07066839832324234 1105 32-33 -0.05209873185146563 1105 34-35 0.0711332049381781 1105 36-37 -0.09498229906183298 1105 38-39 0.17783224139541431 1105 40-41 0.10183720168549826 1105 42-43 -0.15815053412710967 1105 44-45 0.0074733527111305875 1105 46-47 0.0900149154155514 1105 48-49 -0.03182241824590193 1105 50-51 0.03170648487755301 1105 52-53 0.10681802896940695 1105 54-55 0.22447335966516846 1105 56-57 -0.014940743024340009 1105 58-59 0.03249952288444291 1105 60-61 0.09716205478060402 1105 62-63 0.028894519901577098 1105 64-65 -0.0924105657182217 1105 66-67 0.24026435663302337 1105 68-69 0.2682321467112736 1105 70-71 0.09939924037829329 1105 72-73 0.07062819537378928 1105 74-75 -0.2541385446048494 1105 76-77 -0.19566585396815483 1105 78-79 -0.10335211683710455 1105 80-81 -0.01509031553889173 1105 82-83 -0.06402069485643125 1105 84-85 -0.11906683869436208 1105 86-87 -0.18991358867765484 1105 88-89 0.15169066117504926 1105 90-91 0.428997633922382 1105 92-93 0.3206572348369754 1105 94-95 0.2058324209390996 1105 96-97 0.21652482005061913 1105 98-99 0.5205037753431903 1105 100-101 0.9276498591938243 1106 1 -0.04666041879041316 1106 2 0.17441809159340949 1106 3 -0.015157470122463934 1106 4 0.069360350970463 1106 5 -0.29237350931411044 1106 6 -0.16455356275168498 1106 7 0.007371796926506136 1106 8 -0.1439937281932302 1106 9 -0.19116344570029753 1106 10-11 -0.06794731893735673 1106 12-13 -0.03948223128515593 1106 14-15 0.19165317118140024 1106 16-17 0.16325593447349718 1106 18-19 0.037513774107168274 1106 20-21 0.039365813846984565 1106 22-23 -0.08359115654775451 1106 24-25 -0.005591547304533151 1106 26-27 0.03735192460049319 1106 28-29 0.08990232566491585 1106 30-31 0.31211963540900456 1106 32-33 -0.039332813337942696 1106 34-35 -0.15576867773091863 1106 36-37 -0.026666488858857917 1106 38-39 0.1480159075026748 1106 40-41 0.31343444678103083 1106 42-43 0.4158979799358349 1106 44-45 0.2635820501439099 1106 46-47 -0.14742916479873003 1106 48-49 0.2043743043797619 1106 50-51 0.3512857729034451 1106 52-53 0.2139552844864525 1106 54-55 0.44572226828174166 1106 56-57 0.29721147381285107 1106 58-59 0.275737738121812 1106 60-61 0.6082379627854735 1106 62-63 0.20353373163083077 1106 64-65 0.33704206083433164 1106 66-67 0.37448012030516153 1106 68-69 0.43337523339630835 1106 70-71 0.38007953169271147 1106 72-73 0.5146186591660005 1106 74-75 0.27077680238101465 1106 76-77 -0.07060725473808915 1106 78-79 0.2587540919800553 1106 80-81 0.0033995931944446056 1106 82-83 0.48567755178489946 1106 84-85 0.09204723505440171 1106 86-87 0.2590458284146706 1106 88-89 0.11265311758506158 1106 90-91 0.3706471782861378 1106 92-93 0.28956827379914785 1106 94-95 0.41974444454260507 1106 96-97 0.4293472029523677 1106 98-99 0.6279264025754046 1106 100-101 0.5953063953824405 1107 1 -0.04600821616179118 1107 2 0.03416287947619878 1107 3 -0.02477539496230108 1107 4 -0.07607257948326662 1107 5 -0.02084119088512182 1107 6 0.01930290863946027 1107 7 -0.09581518984231252 1107 8 -0.05046181704689445 1107 9 0.045751223079832926 1107 10-11 0.14244581890029906 1107 12-13 -0.2053040616354167 1107 14-15 0.02327412862586442 1107 16-17 -0.03786326725250433 1107 18-19 -0.27625692025471693 1107 20-21 -0.29292316970760623 1107 22-23 -0.17001694854740634 1107 24-25 0.023561497408500998 1107 26-27 -0.1609720247203299 1107 28-29 -0.15987339368431464 1107 30-31 0.0047196122929662465 1107 32-33 -0.08875780177992709 1107 34-35 0.035654857702667186 1107 36-37 0.17776876420676757 1107 38-39 0.004199036093915254 1107 40-41 -0.1380493829968401 1107 42-43 -0.15397576244136957 1107 44-45 0.08157555013291073 1107 46-47 0.5181690728782158 1107 48-49 0.2966472167809613 1107 50-51 0.4156350563061224 1107 52-53 0.28374922147872184 1107 54-55 0.27836212033906804 1107 56-57 -0.247289659973994 1107 58-59 0.3992076762588752 1107 60-61 0.6527611118509284 1107 62-63 0.7107052086358756 1107 64-65 0.6801859526952789 1107 66-67 0.6930941759597857 1107 68-69 0.4370579767201761 1107 70-71 0.2384690039864843 1107 72-73 0.3229144289302148 1107 74-75 0.17236919500149384 1107 76-77 0.021521182397282956 1107 78-79 0.3067625348394003 1107 80-81 0.7050423457647241 1107 82-83 0.47331322093929273 1107 84-85 0.3926651764056963 1107 86-87 0.6916020224133064 1107 88-89 0.8400303548877517 1107 90-91 0.5852424824272084 1107 92-93 0.7761946800250641 1107 94-95 0.8919178203654283 1107 96-97 0.8421646357507306 1107 98-99 1.3305990573978512 1107 100-101 0.8001792021103107 1108 1 -0.1380937280524961 1108 2 -0.20704347910028886 1108 3 -0.1785745179172693 1108 4 -0.19916247659914177 1108 5 -0.4582315602560101 1108 6 -0.268163358859276 1108 7 -0.09098511731743741 1108 8 0.08981478946348176 1108 9 -0.1312474276201172 1108 10-11 -0.18373271036264072 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0.020524845377888568 2316 86-87 -0.5983675218897702 2316 88-89 -0.023316605235208954 2316 90-91 -0.24717113155395154 2316 92-93 -0.4346354156115133 2316 94-95 0.08630504178119125 2316 96-97 -0.4035934478174248 2316 98-99 -0.7181442893132193 2316 100-101 -1.1903656560002958 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 12.0 4 33.0 5 67.0 6 133.0 7 364.0 8 795.0 9 1474.0 10 2336.0 11 3494.0 12 4854.0 13 6319.0 14 8273.0 15 10340.0 16 12625.0 17 14886.0 18 13563.0 19 11448.0 20 11555.0 21 11858.0 22 13182.0 23 14701.0 24 17284.0 25 20270.0 26 24447.0 27 29478.0 28 34922.0 29 41484.0 30 48934.0 31 58065.0 32 69021.0 33 83729.0 34 102470.0 35 125720.0 36 148282.0 37 134858.0 38 44467.0 39 996.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.99628116818725 21.06977004320734 15.63116716632828 22.302781622277127 2 16.51890515956734 22.273926669228715 37.87732880803548 23.329839363168468 3 20.082345141343776 25.29028925528148 30.597991871989606 24.029373731385142 4 13.915265353794707 18.3296782490386 36.794880101105754 30.960176296060943 5 13.431801258332749 38.40914718623555 34.14317018555482 14.015881369876872 6 31.50980973393992 37.67069185560441 16.571909352484784 14.247589057970886 7 27.932570189093862 33.54361513105928 20.730921541932403 17.792893137914447 8 26.377200814383418 35.160311766136346 19.391484474706722 19.071002944773515 9 26.163522905685394 17.09257505575875 20.383339738304418 36.36056230025144 10-11 25.088718377229164 26.999497221851193 27.248445625630414 20.66333877528923 12-13 26.14988779471914 24.43013163319146 27.724622458185394 21.695358113904003 14-15 22.701933667578164 26.011498874164722 26.366689476258053 24.91987798199906 16-17 21.80878351152301 29.255606185131473 26.998798262539697 21.93681204080582 18-19 21.693519900740366 27.749387171153643 29.58450115465652 20.972591773449466 20-21 22.52418921101725 26.543254800122835 29.712037005809673 21.220518983050248 22-23 22.128281979300997 26.5624320483953 29.534907044423598 21.774378927880107 24-25 22.359448297040238 26.830308428065475 29.563795036874424 21.246448238019862 26-27 22.105414510082895 26.95228615261313 29.65308420140314 21.289215135900836 28-29 21.671530540333762 27.497616879416757 29.63479231791982 21.196060262329652 30-31 21.908270999669842 26.90710834519655 29.551969767433604 21.632650887700002 32-33 21.78383813820237 27.165474879275504 29.450298605089554 21.600388377432573 34-35 22.52969636613935 26.795162308181848 29.260606415639952 21.41453491003885 36-37 22.610224690028772 26.651866928398942 29.1743760964995 21.563532285072785 38-39 22.162255718847288 27.01426133728606 29.465516055376682 21.35796688848997 40-41 22.16476980499756 26.99920433431449 29.386378895733184 21.449646964954766 42-43 22.094824089696015 27.272065669156742 28.755550309344045 21.877559931803194 44-45 22.381262170217074 27.092389809057394 28.630651995249956 21.89569602547558 46-47 22.613916939281577 27.47181697318463 28.114865692412284 21.79940039512151 48-49 22.738003633500988 27.67540251185503 27.826058787454393 21.760535067189593 50-51 22.360606763900716 27.70779906304208 27.98483933394957 21.946754839107633 52-53 22.754429368627754 27.834873581326203 27.61077027191941 21.79992677812663 54-55 22.221413575181913 27.578069078081725 27.940052036437045 22.260465310299317 56-57 22.692532133989808 27.8490140695066 27.584309427079752 21.87414436942384 58-59 22.839978149166992 27.35068412544082 27.66540002245435 22.143937702937837 60-61 22.447169191717478 27.57018221192676 28.102972370885144 21.879676225470618 62-63 22.417970020675856 27.486106260315186 28.15373356948512 21.942190149523842 64-65 22.085803261125715 27.771271187531372 27.88949931011405 22.253426241228862 66-67 22.34245460443576 27.709732876426614 27.54540443966355 22.40240807947408 68-69 22.23483535643963 27.578267875456593 27.728264569316448 22.458632198787335 70-71 22.451413012029374 27.553423942659457 27.679229140300087 22.315933905011086 72-73 22.497675791173997 27.313702736464673 27.790082340228672 22.398539132132658 74-75 22.263090042093165 27.962642039465948 27.260926582154895 22.513341336285993 76-77 22.42558826453088 27.468971442366406 27.473453542367825 22.631986750734885 78-79 22.29305383715543 27.626755800598918 27.448357761734776 22.631832600510876 80-81 22.167849050173118 27.938303633954302 27.405942597669792 22.48790471820279 82-83 22.22617186910604 27.70144752339389 27.426170449008286 22.646210158491787 84-85 21.896077176582526 27.682590091269528 27.27914203271076 23.142190699437187 86-87 22.018730110407624 27.721193568586173 27.205389954721294 23.054686366284912 88-89 21.93903658399656 27.60315863331814 27.10880668970595 23.34899809297935 90-91 21.94983936186785 27.61085229413621 27.3095919982283 23.129716345767644 92-93 22.02449577314784 27.772482193969246 27.237968448379153 22.96505358450376 94-95 21.754816652998628 27.582150249042346 27.047277219814042 23.61575587814498 96-97 21.770087674104566 27.58639052230371 27.088311790895688 23.555210012696037 98-99 21.811930279855986 27.637770151801117 26.651442325827222 23.89885724251567 100-101 21.63861005848597 27.65295929058859 26.682231564316595 24.026199086608848 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 264.0 1 193.5 2 147.5 3 192.0 4 184.0 5 176.5 6 284.0 7 520.0 8 769.0 9 902.5 10 904.0 11 903.5 12 957.5 13 1097.0 14 1230.0 15 1311.5 16 1478.0 17 1645.0 18 1743.5 19 1852.0 20 2062.0 21 2350.0 22 2693.5 23 3193.5 24 3809.5 25 4709.0 26 6258.5 27 8027.5 28 9507.5 29 11384.5 30 13811.5 31 16307.0 32 19439.0 33 22174.5 34 24590.5 35 26942.0 36 29577.0 37 32092.0 38 33253.0 39 33485.5 40 34144.0 41 35966.5 42 37240.0 43 38162.0 44 38959.5 45 40029.5 46 40267.0 47 42050.5 48 48999.5 49 48884.0 50 42467.0 51 38956.0 52 36188.5 53 34981.5 54 33804.0 55 31335.5 56 28322.5 57 25464.0 58 23268.5 59 20599.0 60 17257.5 61 14327.5 62 11951.0 63 9492.5 64 7238.5 65 5573.5 66 4231.0 67 3433.5 68 2585.5 69 1809.0 70 1356.5 71 1065.0 72 910.0 73 800.5 74 669.0 75 522.0 76 375.5 77 240.0 78 155.5 79 117.0 80 74.0 81 58.0 82 51.0 83 34.0 84 19.0 85 8.0 86 2.5 87 1.5 88 1.0 89 0.0 90 1.0 91 1.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027779207662446237 2 0.0034613070250332375 3 0.001863780705787128 4 8.875146218033941E-5 5 0.0011537690083444124 6 3.5500584872135766E-4 7 0.0 8 0.0 9 0.0011537690083444124 10-11 2.2187865545084858E-4 12-13 0.0011093932772542428 14-15 0.002041283630147807 16-17 0.0035500584872135772 18-19 0.0 20-21 0.0 22-23 0.001863780705787128 24-25 0.0011093932772542428 26-27 0.005014457613189177 28-29 0.006035099428263081 30-31 8.875146218033943E-4 32-33 2.6625438654101827E-4 34-35 0.007898880134050209 36-37 0.012913337747239385 38-39 0.008387013176042075 40-41 0.0011093932772542428 42-43 2.6625438654101827E-4 44-45 0.0014200233948854306 46-47 0.0 48-49 4.4375731090169716E-4 50-51 9.31890352893564E-4 52-53 0.002618168134320013 54-55 0.002795671058680692 56-57 0.0012868962016149216 58-59 0.0010206418150739034 60-61 0.0028400467897708613 62-63 0.005813220772812232 64-65 0.009452030722206147 66-67 0.0031063011763118797 68-69 0.004481948840107141 70-71 0.0 72-73 0.0012868962016149216 74-75 0.006611983932435287 76-77 0.0033725555628528977 78-79 0.004260070184656292 80-81 0.005591342117361384 82-83 0.005236336268640026 84-85 0.007011365512246814 86-87 0.004748203226648159 88-89 0.010250793881829203 90-91 0.012558331898518028 92-93 0.0015087748570657702 94-95 0.002307538016688825 96-97 0.0011093932772542428 98-99 0.0033281798317627283 100-101 0.005458214924090874 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1126742.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.65791101111808 #Duplication Level Percentage of deduplicated Percentage of total 1 78.59153629317363 45.31423805818863 2 12.52459447272142 14.44283907117026 3 3.953425281811238 6.838387292633303 4 1.696056273281589 3.911642466988737 5 0.8560237935421804 2.4678271855727374 6 0.5049670113726596 1.7469205803165035 7 0.33135220277539285 1.3373553074673088 8 0.2279262594310196 1.0513401586696591 9 0.15981982019678975 0.8293389273647456 >10 1.008323395507963 10.956580462400229 >50 0.095747262385676 3.758524274682784 >100 0.04622202768082278 4.719207886044073 >500 0.0029273929336155703 1.1064455707923875 >1k 0.0010785131860688941 1.5193527577087278 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3990 0.3541183340995543 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3871 0.34355691010009387 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3723 0.33042169369740365 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2288 0.2030633454686166 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1145 0.10162042419648865 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.7750292436067883E-4 0.0 16-17 0.0 0.0 0.0 1.7750292436067883E-4 0.0 18-19 0.0 0.0 0.0 1.7750292436067883E-4 0.0 20-21 0.0 0.0 0.0 2.2187865545084855E-4 0.0 22-23 0.0 0.0 0.0 3.5500584872135766E-4 0.0 24-25 0.0 0.0 0.0 4.881330419918668E-4 0.0 26-27 0.0 0.0 0.0 0.0010206418150739034 0.0 28-29 0.0 0.0 0.0 0.002751295327590522 0.0 30-31 0.0 0.0 0.0 0.005280711999730195 0.0 32-33 0.0 0.0 0.0 0.011892695932165482 0.0 34-35 0.0 0.0 0.0 0.023652264671060456 0.0 36-37 0.0 0.0 0.0 0.04255632611547275 0.0 38-39 0.0 0.0 0.0 0.07259869606351764 0.0 40-41 0.0 0.0 0.0 0.10903117128854697 0.0 42-43 0.0 0.0 0.0 0.15287439360563465 0.0 44-45 0.0 0.0 0.0 0.2067465311491007 0.0 46-47 0.0 0.0 0.0 0.2804102447587824 0.0 48-49 0.0 0.0 0.0 0.3580677741665794 0.0 50-51 0.0 0.0 0.0 0.4395859921792211 0.0 52-53 0.0 0.0 0.0 0.5222579792002073 0.0 54-55 0.0 0.0 0.0 0.6151807601030227 0.0 56-57 0.0 0.0 0.0 0.7370808934077189 0.0 58-59 0.0 0.0 0.0 0.871761237266384 0.0 60-61 0.0 0.0 0.0 1.025789399880363 0.0 62-63 0.0 0.0 0.0 1.1878495698216627 0.0 64-65 0.0 0.0 0.0 1.3617580599640378 0.0 66-67 0.0 0.0 0.0 1.5663745560208104 0.0 68-69 0.0 0.0 0.0 1.8080891632689648 0.0 70-71 0.0 0.0 0.0 2.090185685809174 0.0 72-73 0.0 0.0 0.0 2.373657856013178 0.0 74-75 0.0 0.0 0.0 2.670132115426602 0.0 76-77 0.0 0.0 0.0 3.015153424652671 0.0 78-79 0.0 0.0 0.0 3.394787804129073 0.0 80-81 0.0 0.0 0.0 3.799095090091609 0.0 82-83 0.0 0.0 0.0 4.205310532491023 0.0 84-85 0.0 0.0 0.0 4.653194786384105 0.0 86-87 0.0 0.0 0.0 5.0999252712688445 0.0 88-89 0.0 0.0 0.0 5.572615558841331 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACACCG 70 0.00839307 27.141817 5 GAGTACT 4905 0.0 22.709108 12-13 GTACATG 4820 0.0 21.788849 1 GTACTTT 5190 0.0 21.553598 14-15 ACATGGG 4900 0.0 20.260328 3 TACATGG 5205 0.0 20.077862 2 GTATCAA 10385 0.0 19.493605 1 CATGGGG 2100 0.0 19.225452 4 AGTACTT 5180 0.0 19.027624 12-13 GTATTAG 450 0.0 19.008553 1 GATCCGT 125 0.0060339533 18.998428 6 TAAGGTG 585 0.0 18.674498 5 ACTTTTT 6025 0.0 18.605509 16-17 GCGTGCG 235 2.5271802E-6 18.191599 9 GGTATCA 8275 0.0 17.458004 1 GTGTAAG 410 3.274181E-11 17.38587 1 TATCAAC 11715 0.0 17.23305 2 TCAACGC 11725 0.0 17.176315 4 ATCAACG 11755 0.0 17.13324 3 GTACACG 195 2.267456E-4 17.058956 1 >>END_MODULE