FastQCFastQC Report
Thu 26 May 2016
SRR936400_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936400_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1149869
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47640.4143080646578002No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36250.3152533027675326No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22420.1949787323599471No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19130.16636677743290756No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18470.16062699316182974No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT14380.12505772396681708No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC12760.11096916257417148No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG47050.026.4680711
TACATGG49400.025.016532
GAGTACT44150.024.16521612-13
ACATGGG48550.024.085983
GTATCAA82900.022.4756451
GTACTTT46400.022.1209114-15
GGTATCA62600.022.0952681
CATGGGG23500.020.0264844
ATCAACG92900.019.8533823
TCAACGC92900.019.8030764
TATCAAC94900.019.6336272
CAACGCA94250.019.5707135
TCTATAC3655.456968E-1219.5351563
AACGCAG95000.019.4662486
CATGGGA25950.019.0516834
ACACCGT1507.6778117E-419.0158756
GTATTAC2001.2774315E-519.0125621
AGTACTT46200.018.97839412-13
GTATAGG3901.4551915E-1118.2813111
ACTTTTT57350.018.02080516-17
Produced by FastQC (version 0.11.5)