##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936400_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1149869 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06335939137415 33.0 31.0 34.0 31.0 34.0 2 32.1894320135598 33.0 31.0 34.0 31.0 34.0 3 32.17918041098595 34.0 31.0 34.0 30.0 34.0 4 35.82038214787946 37.0 35.0 37.0 35.0 37.0 5 35.84391352406231 37.0 35.0 37.0 35.0 37.0 6 35.89306521003697 37.0 35.0 37.0 35.0 37.0 7 35.79897710086975 37.0 35.0 37.0 35.0 37.0 8 35.794246996831816 37.0 35.0 37.0 35.0 37.0 9 37.429455877147745 39.0 37.0 39.0 35.0 39.0 10-11 37.3441800761652 39.0 37.0 39.0 35.0 39.0 12-13 37.29613242899843 39.0 37.0 39.0 34.5 39.0 14-15 38.48845738079729 40.0 38.0 41.0 34.0 41.0 16-17 38.42087707382319 40.0 38.0 41.0 34.0 41.0 18-19 38.32940187099574 40.0 38.0 41.0 34.0 41.0 20-21 38.319287675378675 40.0 38.0 41.0 34.0 41.0 22-23 38.2779620982912 40.0 38.0 41.0 34.0 41.0 24-25 38.388033332492654 40.0 38.0 41.0 34.0 41.0 26-27 38.31257256261365 40.0 38.0 41.0 34.0 41.0 28-29 38.20639959856297 40.0 38.0 41.0 34.0 41.0 30-31 38.01969572186049 40.0 38.0 41.0 34.0 41.0 32-33 37.871440572795684 40.0 37.0 41.0 33.5 41.0 34-35 37.700463270163816 40.0 37.0 41.0 33.0 41.0 36-37 37.49801846993005 40.0 37.0 41.0 33.0 41.0 38-39 37.32720031586207 40.0 37.0 41.0 33.0 41.0 40-41 37.13001872387203 39.0 36.5 41.0 32.0 41.0 42-43 36.87355168284387 39.0 36.0 41.0 31.0 41.0 44-45 36.74618934852579 39.0 36.0 41.0 31.0 41.0 46-47 36.626203506660325 39.0 36.0 41.0 31.0 41.0 48-49 36.415543857604646 39.0 36.0 41.0 30.0 41.0 50-51 35.43076559155869 38.0 35.0 40.0 29.0 40.5 52-53 35.54518949549905 38.5 35.0 39.5 29.5 40.5 54-55 35.99564863475752 39.0 35.0 40.5 30.0 41.0 56-57 35.86152900895667 39.0 35.0 41.0 29.0 41.0 58-59 35.68252513982027 39.0 35.0 40.5 29.0 41.0 60-61 35.417711060999125 38.5 35.0 40.0 28.0 41.0 62-63 35.09849861158097 38.0 35.0 40.0 28.0 41.0 64-65 34.759948307154986 37.5 34.0 40.0 27.5 41.0 66-67 34.40588319191143 37.0 34.0 40.0 26.5 41.0 68-69 33.9816853050217 36.5 34.0 39.0 26.0 41.0 70-71 33.5920287441439 36.0 33.5 39.0 26.0 40.5 72-73 33.186939990555445 35.5 33.0 38.5 25.5 40.0 74-75 32.76437011520443 35.0 33.0 37.5 24.5 39.5 76-77 32.321618375658446 35.0 32.5 37.0 23.5 39.0 78-79 31.87918449840808 35.0 32.0 36.5 22.5 39.0 80-81 31.45309726586246 35.0 32.0 36.0 20.5 38.5 82-83 31.059612877640845 35.0 31.0 35.5 19.5 37.0 84-85 30.705892584285685 34.0 31.0 35.0 18.5 37.0 86-87 30.38942349084983 34.0 31.0 35.0 17.5 36.0 88-89 30.06925919387339 34.0 31.0 35.0 10.0 36.0 90-91 29.747912153471397 34.0 30.5 35.0 6.0 36.0 92-93 29.36343139957682 34.0 30.0 35.0 2.0 35.0 94-95 28.944687612241047 34.0 29.5 35.0 2.0 35.0 96-97 28.420043500607463 34.0 29.0 35.0 2.0 35.0 98-99 27.77675674359427 34.0 29.0 35.0 2.0 35.0 100-101 25.36726401007419 31.5 22.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.389215307636281 1101 2 0.33814268869975805 1101 3 0.4981752470545544 1101 4 0.47517795443489064 1101 5 0.36826070671104816 1101 6 0.37688203020363176 1101 7 0.3848240584487286 1101 8 0.3037698782260847 1101 9 0.5137187787523061 1101 10-11 0.529282911214267 1101 12-13 0.4735821688370834 1101 14-15 0.7588880355357404 1101 16-17 0.792214224545063 1101 18-19 0.8607105825202126 1101 20-21 0.671804263940821 1101 22-23 0.7199445879082162 1101 24-25 0.5666923150406333 1101 26-27 0.4996624941220631 1101 28-29 0.4717797949507698 1101 30-31 0.33459746197839735 1101 32-33 0.3275284563062968 1101 34-35 0.3721962116781299 1101 36-37 0.39968891071668367 1101 38-39 0.4998494565711411 1101 40-41 0.4384905616522019 1101 42-43 0.5046523092397521 1101 44-45 0.4515394581680212 1101 46-47 0.47770752646064096 1101 48-49 0.5848179425705453 1101 50-51 0.4743663359829 1101 52-53 0.4384708756562432 1101 54-55 0.5337557175584067 1101 56-57 0.5558170856669804 1101 58-59 0.4779011085930307 1101 60-61 0.4339124091212554 1101 62-63 0.41317207417560553 1101 64-65 0.40086664622445767 1101 66-67 0.39769296875190463 1101 68-69 0.4458928804318205 1101 70-71 0.3957454664704372 1101 72-73 0.43594418356995135 1101 74-75 0.3401711410509236 1101 76-77 0.4956562258744057 1101 78-79 0.5741607227985597 1101 80-81 0.5723282364331546 1101 82-83 0.5286264179952305 1101 84-85 0.5897924546091851 1101 86-87 0.5132364671629119 1101 88-89 0.5303482912933042 1101 90-91 0.49831109142107977 1101 92-93 0.6041899279332874 1101 94-95 0.6053079154049676 1101 96-97 0.4384308648092272 1101 98-99 0.5076828991168334 1101 100-101 0.8759444485601904 1102 1 0.29347240167897226 1102 2 0.37910271393966255 1102 3 0.5112024040098291 1102 4 0.23950600895734908 1102 5 0.28708020667227885 1102 6 0.29184843493504786 1102 7 0.3913278778551117 1102 8 0.24306765289046695 1102 9 0.45118933443114884 1102 10-11 0.46203422066773214 1102 12-13 0.370444426899013 1102 14-15 0.6276908247585169 1102 16-17 0.42854283520090064 1102 18-19 0.492993991296963 1102 20-21 0.5645610277793125 1102 22-23 0.4128243427817395 1102 24-25 0.5358860018573353 1102 26-27 0.37680541237848075 1102 28-29 0.2653146107716964 1102 30-31 0.2649473714730419 1102 32-33 0.4829986162748554 1102 34-35 0.2924714174875831 1102 36-37 0.37759103124255233 1102 38-39 0.360691517366142 1102 40-41 0.492854337049188 1102 42-43 0.4579269455472357 1102 44-45 0.509614086989103 1102 46-47 0.5092438983855274 1102 48-49 0.7376173254763998 1102 50-51 0.6360992796663751 1102 52-53 0.5554738515045514 1102 54-55 0.6536175665414561 1102 56-57 0.6007906292258554 1102 58-59 0.4897673371264091 1102 60-61 0.5276497590922489 1102 62-63 0.5930407144027683 1102 64-65 0.6795278353338716 1102 66-67 0.5549644126399187 1102 68-69 0.6434838603762572 1102 70-71 0.48600952287519306 1102 72-73 0.6469084332845298 1102 74-75 0.5485155480336061 1102 76-77 0.7088230846031607 1102 78-79 0.597299748121344 1102 80-81 0.6510263228530739 1102 82-83 0.5611230557946563 1102 84-85 0.670924159291669 1102 86-87 0.45901774097571746 1102 88-89 0.6307992138597562 1102 90-91 0.7741596627197858 1102 92-93 0.8020800969390116 1102 94-95 0.78629711090991 1102 96-97 0.623207916821066 1102 98-99 0.8494920972420239 1102 100-101 1.35036806176943 1103 1 0.23579858977197787 1103 2 0.29566700274800795 1103 3 0.3320903014786083 1103 4 0.36753482322596653 1103 5 0.2625287930012874 1103 6 0.2886226146681423 1103 7 0.40077743575407254 1103 8 0.1637470421473992 1103 9 0.35829549986234355 1103 10-11 0.3569067418795271 1103 12-13 0.33753738925740606 1103 14-15 0.6165520321972693 1103 16-17 0.6755363523575539 1103 18-19 0.7644378188910181 1103 20-21 0.6354579495911779 1103 22-23 0.4445320605223131 1103 24-25 0.4066587854174841 1103 26-27 0.4908310464074077 1103 28-29 0.5206368318246604 1103 30-31 0.4761189881703345 1103 32-33 0.5695666922481948 1103 34-35 0.5636647048446903 1103 36-37 0.542205603078763 1103 38-39 0.3125717316009897 1103 40-41 0.5511341565378487 1103 42-43 0.6370815695594487 1103 44-45 0.6792870325591593 1103 46-47 0.8658392859978861 1103 48-49 1.076809955738966 1103 50-51 1.026199452933767 1103 52-53 1.0076657879633828 1103 54-55 0.8541750262164101 1103 56-57 1.0901916147453008 1103 58-59 0.9252174346504987 1103 60-61 0.8351504259759039 1103 62-63 0.8321896301355949 1103 64-65 1.016597603600978 1103 66-67 0.9042445759713473 1103 68-69 1.014465886332104 1103 70-71 0.8831044797663665 1103 72-73 0.935544552192944 1103 74-75 0.8549857573004758 1103 76-77 0.8859039451645216 1103 78-79 0.8398916801750786 1103 80-81 0.9122513820160485 1103 82-83 0.7360146719046057 1103 84-85 0.9091518272545791 1103 86-87 0.7605378686258106 1103 88-89 0.7076271174956794 1103 90-91 0.8954283262849323 1103 92-93 0.7792091994971564 1103 94-95 0.9227594032276549 1103 96-97 1.0118068370813589 1103 98-99 1.3501239542132204 1103 100-101 1.5751948861551028 1104 1 0.22425568418806918 1104 2 0.21619630419319336 1104 3 0.327130277788946 1104 4 0.2974456386033495 1104 5 0.3080580944464728 1104 6 0.2629522784258569 1104 7 0.23504703343620292 1104 8 0.3158936277582498 1104 9 0.5088932574729412 1104 10-11 0.5040562886985498 1104 12-13 0.5866831387624174 1104 14-15 0.49218346629297116 1104 16-17 0.6200470852856057 1104 18-19 0.5660851360944719 1104 20-21 0.530985726885099 1104 22-23 0.35057938469331873 1104 24-25 0.5310669066760596 1104 26-27 0.17928327805518052 1104 28-29 0.1938421074202381 1104 30-31 0.26453304345783124 1104 32-33 0.4719756496126024 1104 34-35 0.38813146963767053 1104 36-37 0.4878648928533025 1104 38-39 0.42611483169563513 1104 40-41 0.4455859556169699 1104 42-43 0.6862695598281121 1104 44-45 0.6476166208350946 1104 46-47 0.7999861532138297 1104 48-49 0.9233983245777679 1104 50-51 0.8567004051654195 1104 52-53 0.6500363947788088 1104 54-55 0.8396582111990156 1104 56-57 0.831950674010109 1104 58-59 0.8843807046372021 1104 60-61 0.8833310248367141 1104 62-63 0.866421704665143 1104 64-65 0.9559251335469199 1104 66-67 0.9210664883312063 1104 68-69 0.8779843096919748 1104 70-71 0.8720159164168351 1104 72-73 0.8886623850530739 1104 74-75 0.8012169666927278 1104 76-77 0.798511288960789 1104 78-79 0.5838092279463325 1104 80-81 0.6888093549239009 1104 82-83 0.7686130014348187 1104 84-85 0.8362981225509003 1104 86-87 0.680460412710076 1104 88-89 0.8914060110489537 1104 90-91 1.0297006093764338 1104 92-93 0.9959358886922267 1104 94-95 0.9977959102793967 1104 96-97 0.9326409687796406 1104 98-99 0.7667075183466032 1104 100-101 1.2262096080047513 1105 1 0.2854896819193904 1105 2 0.23663464481869667 1105 3 0.42165507117737633 1105 4 0.3463335822105478 1105 5 0.3594881706091684 1105 6 0.3038694717494721 1105 7 0.3740945946094314 1105 8 0.32073482043366397 1105 9 0.2082329106896026 1105 10-11 0.43735822517562184 1105 12-13 0.4372644870041924 1105 14-15 0.711039810483534 1105 16-17 0.5875867871167557 1105 18-19 0.4040571017402357 1105 20-21 0.4123258662466327 1105 22-23 0.3655384674999951 1105 24-25 0.21742244062517102 1105 26-27 0.2676299803724689 1105 28-29 0.4578390285027183 1105 30-31 0.2880503016007623 1105 32-33 0.3549463998961855 1105 34-35 0.2928470748880301 1105 36-37 0.44758070066403377 1105 38-39 0.33387897040896775 1105 40-41 0.31763363832642 1105 42-43 0.14444084487104902 1105 44-45 0.41098054078324253 1105 46-47 0.5396251906469871 1105 48-49 0.806926115859568 1105 50-51 0.8531353427768167 1105 52-53 0.8089956396337712 1105 54-55 0.8903286860005011 1105 56-57 0.8242128681439667 1105 58-59 0.817692769132421 1105 60-61 0.8165772572139645 1105 62-63 0.962804989383983 1105 64-65 0.9421358368364992 1105 66-67 0.8489398070801855 1105 68-69 0.966103131600903 1105 70-71 0.9265147820787973 1105 72-73 0.940062077161322 1105 74-75 0.883132377485147 1105 76-77 0.9488212063161612 1105 78-79 1.0448720621709313 1105 80-81 1.0814827465946202 1105 82-83 0.8634568273949554 1105 84-85 0.7979433178190902 1105 86-87 0.9685265689246272 1105 88-89 0.9162753596148256 1105 90-91 0.9362088738392451 1105 92-93 1.0502018099367554 1105 94-95 0.9621047743208813 1105 96-97 1.1567588183988278 1105 98-99 1.4881566351834188 1105 100-101 1.768615619996325 1106 1 0.20625542010937536 1106 2 0.08216316231691323 1106 3 0.15440300506167404 1106 4 0.2891799754357294 1106 5 0.18945705133562285 1106 6 0.16060858000373202 1106 7 0.2813400287488008 1106 8 0.3727167610519686 1106 9 0.4399720189438625 1106 10-11 0.4625909820371632 1106 12-13 0.4980121147972767 1106 14-15 0.332620516533936 1106 16-17 0.3554484849026949 1106 18-19 0.4357094841501947 1106 20-21 0.2191517003451935 1106 22-23 0.28598408529409625 1106 24-25 0.008101963122882694 1106 26-27 -0.055251613342456096 1106 28-29 0.07908175883636659 1106 30-31 0.11666217793990796 1106 32-33 0.26796165344174483 1106 34-35 0.3590597062521752 1106 36-37 0.07679009256282399 1106 38-39 0.2378135179041223 1106 40-41 0.21507331284942666 1106 42-43 0.35317719830437255 1106 44-45 0.24175737477977322 1106 46-47 0.20841026359872927 1106 48-49 0.27177919207628065 1106 50-51 0.13155846286661443 1106 52-53 0.28756032691058664 1106 54-55 0.2863456740629502 1106 56-57 0.4809851362798696 1106 58-59 0.41167488830393495 1106 60-61 0.5035875112696715 1106 62-63 0.5966108510307606 1106 64-65 0.2862034064722536 1106 66-67 0.17125959587724537 1106 68-69 0.2780948926456901 1106 70-71 0.09320823172679837 1106 72-73 0.23695907746741085 1106 74-75 0.4051913510592726 1106 76-77 0.16803990848941197 1106 78-79 0.4042505694661074 1106 80-81 0.3261121211482383 1106 82-83 0.38195557752249343 1106 84-85 0.604433726961016 1106 86-87 0.8135659781685831 1106 88-89 0.6769675083751601 1106 90-91 0.7486680616772183 1106 92-93 0.8569281643903857 1106 94-95 0.9275674822078308 1106 96-97 0.9479822904935098 1106 98-99 0.982834474179441 1106 100-101 1.5074369137418628 1107 1 0.3356331126395524 1107 2 0.27195095668752245 1107 3 0.41568537250926596 1107 4 0.32135485479515324 1107 5 0.3912480120456152 1107 6 0.30318671095012917 1107 7 0.36904458492849557 1107 8 0.28274096528085835 1107 9 0.4145476837990145 1107 10-11 0.5269791782893165 1107 12-13 0.4129022824001467 1107 14-15 0.3113782611260447 1107 16-17 0.41441584725065894 1107 18-19 0.6399140174957125 1107 20-21 0.42140429202441254 1107 22-23 0.317670693809724 1107 24-25 0.338397209046974 1107 26-27 0.25453587657244725 1107 28-29 0.2630407486523154 1107 30-31 0.291568557809299 1107 32-33 0.3303842075206873 1107 34-35 0.3176582966222412 1107 36-37 0.40497279643397377 1107 38-39 0.5691047145672812 1107 40-41 0.46343077429771995 1107 42-43 0.42659174519816645 1107 44-45 0.5343720404112844 1107 46-47 0.494471900657075 1107 48-49 0.6089178412062992 1107 50-51 0.47592146540880975 1107 52-53 0.7126684086873851 1107 54-55 0.5752619639656729 1107 56-57 0.7035361706681371 1107 58-59 0.691553029906288 1107 60-61 0.5910184378806207 1107 62-63 0.6491614287354679 1107 64-65 0.7212241855744139 1107 66-67 0.7597169187981976 1107 68-69 0.5888711024765669 1107 70-71 0.7157295816834122 1107 72-73 0.6683697729964777 1107 74-75 0.7074271976744839 1107 76-77 0.7297549836138515 1107 78-79 0.7285503213347013 1107 80-81 0.7928819221864281 1107 82-83 0.7300926891511814 1107 84-85 0.7884604581722954 1107 86-87 0.8962873555262121 1107 88-89 0.9113078289670185 1107 90-91 0.8325954642133375 1107 92-93 0.8369562504295693 1107 94-95 0.6450668352394509 1107 96-97 0.7894293684798406 1107 98-99 1.0164498096135155 1107 100-101 1.6602356228496546 1108 1 0.1582429949273667 1108 2 0.1279392818894145 1108 3 0.22551616053545587 1108 4 0.2827311469691125 1108 5 0.17633281087314145 1108 6 0.18199715655943294 1108 7 0.3232585495388349 1108 8 0.22203360319309695 1108 9 -0.08440757377366737 1108 10-11 0.25018539184102195 1108 12-13 0.2260079579265195 1108 14-15 0.4784264497287154 1108 16-17 0.557694160507765 1108 18-19 0.43105060877681467 1108 20-21 0.441265741709465 1108 22-23 0.372508044088832 1108 24-25 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-2.4576348580930016 2316 64-65 -2.743245554684023 2316 66-67 -2.410312219840904 2316 68-69 -2.8328054385798396 2316 70-71 -2.365825191940772 2316 72-73 -1.674874707537107 2316 74-75 -2.039802626658286 2316 76-77 -2.5350992062350706 2316 78-79 -2.72889456754082 2316 80-81 -3.5334708357924463 2316 82-83 -2.659605699201382 2316 84-85 -2.5645214070347677 2316 86-87 -3.146822459049126 2316 88-89 -4.770999971299641 2316 90-91 -3.505314172589568 2316 92-93 -2.9060122150134085 2316 94-95 -2.3749016535946375 2316 96-97 -3.555104129259572 2316 98-99 -5.361497052469158 2316 100-101 -6.7936772085104735 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2049.0 3 678.0 4 555.0 5 583.0 6 613.0 7 708.0 8 826.0 9 1049.0 10 1338.0 11 1867.0 12 2557.0 13 3434.0 14 4173.0 15 5549.0 16 7641.0 17 10394.0 18 7898.0 19 4952.0 20 4615.0 21 4754.0 22 5096.0 23 5632.0 24 6686.0 25 7290.0 26 8795.0 27 10665.0 28 13047.0 29 16596.0 30 21262.0 31 27654.0 32 37257.0 33 52224.0 34 76507.0 35 115085.0 36 176836.0 37 252963.0 38 215358.0 39 34679.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.64260420736956 18.616909716390257 14.479683294186039 23.26080278205415 2 15.128220072776493 19.83050714759814 40.84452397335646 24.196748806268904 3 18.95354571714109 24.40761199988519 30.35841180957649 26.280430473397224 4 12.294263471491323 16.39116252772583 38.05801765754795 33.2565563432349 5 13.37060413846855 37.19637370781591 34.79493808302685 14.63808407068869 6 31.33722133972674 36.0852983598343 17.886192299729103 14.691288000709863 7 27.928108272770793 32.84198616127769 21.170547021193265 18.059358544758258 8 26.01017747936833 33.885367610972466 20.405254419580857 19.69920049007835 9 26.08218148084007 16.758898990593433 21.03480055104426 36.12411897752224 10-11 24.59549544493217 26.067457380671755 27.91524123651416 21.421805937881917 12-13 25.781067505686483 23.78865644165641 28.169672704842764 22.260603347814342 14-15 22.663715836441188 25.046215464542627 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22.136907979333824 40-41 21.86792830244471 26.38403068279659 29.80240557299775 21.945635441760956 42-43 21.705551414922457 26.593097714399516 29.178606619466347 22.52274425121168 44-45 21.71047110333435 26.588045808875744 29.176176845184802 22.525306242605105 46-47 22.03508930605827 26.73433252222433 28.737478975811094 22.493099195906304 48-49 22.175680546985713 26.84118272963229 28.46211143825082 22.521025285131174 50-51 21.894836809290943 26.979973927056637 28.60327120644327 22.52191805720915 52-53 22.208177119614703 27.134746888118364 28.219611485502238 22.437464506764695 54-55 21.67987400270988 26.74668178726913 28.688038432304285 22.88540577771671 56-57 22.099292556515252 27.163020738891213 28.189681300384528 22.548005404209004 58-59 22.199156321436227 26.686063475484318 28.3832045261733 22.731575676906157 60-61 22.036711545803765 26.794418996215956 28.82367725983603 22.345192198144247 62-63 21.89812793624234 26.87241162791102 28.789698518246666 22.439761917599974 64-65 21.681927147131557 26.86514205357061 28.67561466971572 22.77731612958211 66-67 21.668239315050027 27.106095063333523 28.27805872863336 22.94760689298309 68-69 21.635102909791083 26.94257124022257 28.599364086544064 22.822961763442283 70-71 21.878598289171126 26.818277482667334 28.377782532012812 22.92534169614872 72-73 21.873189723107007 26.64484041729655 28.459857735068532 23.022112124527915 74-75 21.613597491262166 27.146971732341573 28.242217924637842 22.997212851758416 76-77 21.753144236950078 26.847725772261477 28.3090054912699 23.090124499518545 78-79 21.766655765196244 26.867587145078858 28.39064723421577 22.975109855509128 80-81 21.84569756165444 27.120299542076353 28.151040873925314 22.882962022343893 82-83 21.88524472541364 27.01630980941087 28.184874366156027 22.913571099019464 84-85 21.693638441388572 26.978007451445585 27.961053847304502 23.367300259861338 86-87 21.741348092205847 27.001457566212295 28.194687710351328 23.06250663123053 88-89 21.847735716472325 26.8176189018451 27.959587983190936 23.375057398491634 90-91 21.696114119079002 26.90233615537784 28.18288831304821 23.21866141249495 92-93 21.895435921386905 26.93756126155979 28.192058849365488 22.974943967687818 94-95 21.7567994211001 26.73185088804949 28.068165115192905 23.4431845756575 96-97 21.779269298726646 26.68686511123858 28.089159593079767 23.444705996955012 98-99 21.904875399969644 26.829465600979802 27.601417339752278 23.66424165929828 100-101 21.90465586798023 26.554739458929095 27.90341548615835 23.637189186932325 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 170.0 1 133.5 2 147.0 3 195.0 4 175.5 5 170.5 6 304.0 7 653.0 8 1133.5 9 1363.0 10 1153.5 11 969.0 12 1007.0 13 1084.5 14 1218.5 15 1313.5 16 1414.0 17 1488.0 18 1533.5 19 1656.0 20 1862.0 21 2182.0 22 2559.5 23 3046.0 24 3618.0 25 4554.5 26 6102.0 27 7870.0 28 9274.5 29 11133.0 30 13628.0 31 16279.0 32 19563.0 33 22191.5 34 24666.0 35 27214.0 36 29976.0 37 32730.5 38 33604.0 39 33517.0 40 34232.0 41 36267.5 42 37766.5 43 38742.0 44 39750.5 45 40969.0 46 41376.0 47 42997.0 48 50294.5 49 50266.5 50 42997.0 51 39443.0 52 37196.0 53 36593.0 54 35741.5 55 32920.5 56 29699.0 57 26735.5 58 24121.0 59 21468.0 60 18394.5 61 15160.0 62 12476.0 63 9792.5 64 7232.0 65 5690.0 66 4457.0 67 3549.5 68 2550.0 69 1714.5 70 1373.0 71 1142.5 72 981.5 73 842.5 74 640.5 75 492.0 76 365.5 77 213.5 78 132.5 79 95.0 80 68.5 81 55.0 82 30.5 83 17.0 84 14.0 85 9.0 86 5.5 87 3.5 88 5.5 89 5.5 90 3.5 91 3.0 92 3.0 93 3.0 94 2.0 95 0.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0687034783962347 2 0.028003189928591864 3 0.01374069567924694 4 0.019045647808576455 5 0.023046103512661006 6 0.030525216350732128 7 0.07992214765334138 8 0.05783267485252668 9 0.06765988125603872 10-11 0.056919527354855205 12-13 0.03761298026122976 14-15 0.031481847062578436 16-17 0.04126557025191565 18-19 0.035264886695788825 20-21 0.015349574603715728 22-23 0.012262266397302648 24-25 0.01726283602740834 26-27 0.02161115744489155 28-29 0.005087536058455354 30-31 4.348321417483209E-4 32-33 0.007392146409721455 34-35 0.009870689617686884 36-37 0.003087308206413078 38-39 0.0058702339136023325 40-41 0.0033916907056369032 42-43 0.0015653957102939554 44-45 3.9134892757348885E-4 46-47 2.6089928504899253E-4 48-49 3.043824992238246E-4 50-51 0.0015653957102939554 52-53 1.7393285669932835E-4 54-55 2.6089928504899253E-4 56-57 0.0021306774945667726 58-59 0.0020437110662171083 60-61 0.003478657133986567 62-63 0.0013044964252449625 64-65 3.043824992238246E-4 66-67 3.478657133986567E-4 68-69 0.0023480935654409327 70-71 0.0014784292819442911 72-73 0.015914856387988546 74-75 0.011088219614582184 76-77 0.019654412807024103 78-79 0.015393057817890559 80-81 0.009914172831861716 82-83 0.003435173919811735 84-85 0.0021741607087416045 86-87 6.087649984476492E-4 88-89 0.0011305635685456344 90-91 0.00456573748835737 92-93 0.00747911283807112 94-95 0.008870575691665746 96-97 0.009609790332637891 98-99 0.021785090301590877 100-101 0.019393513521975114 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1149869.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.92778908980281 #Duplication Level Percentage of deduplicated Percentage of total 1 77.76341300497543 44.268991744504696 2 13.0409434809882 14.847841600354702 3 4.120809264185204 7.037656820125222 4 1.7162251749672033 3.908036191645714 5 0.9165022568502039 2.608722358914834 6 0.5185096498288269 1.7710564791889774 7 0.3427603307800867 1.3658811473299872 8 0.2317678006871713 1.055522278026139 9 0.16890439803217214 0.8653818552764039 >10 1.0352079285444693 11.32468277315986 >50 0.0932438938030319 3.6449318520358083 >100 0.04895888166789366 5.128648964786756 >500 0.0015299637167105898 0.5925239644590085 >1k 0.001223970973368472 1.5801219701918758 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4764 0.4143080646578002 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3625 0.3152533027675326 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2242 0.1949787323599471 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1913 0.16636677743290756 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1847 0.16062699316182974 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1438 0.12505772396681708 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1276 0.11096916257417148 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.696642834966418E-5 2 0.0 0.0 0.0 0.0 8.696642834966418E-5 3 0.0 0.0 0.0 0.0 8.696642834966418E-5 4 0.0 0.0 0.0 0.0 8.696642834966418E-5 5 0.0 0.0 0.0 0.0 8.696642834966418E-5 6 0.0 0.0 0.0 0.0 8.696642834966418E-5 7 0.0 0.0 0.0 0.0 8.696642834966418E-5 8 0.0 0.0 0.0 8.696642834966418E-5 8.696642834966418E-5 9 0.0 0.0 0.0 8.696642834966418E-5 8.696642834966418E-5 10-11 0.0 0.0 0.0 8.696642834966418E-5 8.696642834966418E-5 12-13 0.0 0.0 0.0 8.696642834966418E-5 8.696642834966418E-5 14-15 0.0 0.0 0.0 8.696642834966418E-5 8.696642834966418E-5 16-17 0.0 0.0 0.0 2.1741607087416044E-4 8.696642834966418E-5 18-19 0.0 0.0 0.0 2.6089928504899253E-4 8.696642834966418E-5 20-21 0.0 0.0 0.0 2.6089928504899253E-4 8.696642834966418E-5 22-23 0.0 0.0 0.0 3.043824992238246E-4 8.696642834966418E-5 24-25 0.0 0.0 0.0 5.652817842728172E-4 8.696642834966418E-5 26-27 0.0 0.0 0.0 8.261810693218096E-4 8.696642834966418E-5 28-29 0.0 0.0 0.0 0.0017393285669932835 8.696642834966418E-5 30-31 0.0 0.0 0.0 0.005348435343504347 8.696642834966418E-5 32-33 0.0 0.0 0.0 0.011088219614582184 8.696642834966418E-5 34-35 0.0 0.0 0.0 0.020958909232269066 8.696642834966418E-5 36-37 0.0 0.0 0.0 0.03535185312413849 8.696642834966418E-5 38-39 0.0 0.0 0.0 0.05961548663369479 8.696642834966418E-5 40-41 0.0 0.0 0.0 0.0898363204852031 8.696642834966418E-5 42-43 0.0 0.0 0.0 0.12362277789904763 8.696642834966418E-5 44-45 0.0 0.0 0.0 0.16567104600611027 8.696642834966418E-5 46-47 0.0 0.0 0.0 0.22867822334544197 8.696642834966418E-5 48-49 0.0 0.0 0.0 0.29459877603448736 8.696642834966418E-5 50-51 0.0 0.0 0.0 0.3635631537157711 8.696642834966418E-5 52-53 0.0 0.0 0.0 0.4386586645957061 8.696642834966418E-5 54-55 0.0 0.0 0.0 0.5238422811642022 8.696642834966418E-5 56-57 8.696642834966418E-5 0.0 0.0 0.634202678739926 8.696642834966418E-5 58-59 1.7393285669932835E-4 0.0 0.0 0.758521188065771 8.696642834966418E-5 60-61 1.7393285669932835E-4 0.0 0.0 0.896536909856688 8.696642834966418E-5 62-63 1.7393285669932835E-4 0.0 0.0 1.0449451198353898 8.696642834966418E-5 64-65 1.7393285669932835E-4 0.0 0.0 1.2032675026459536 8.696642834966418E-5 66-67 1.7393285669932835E-4 0.0 0.0 1.385983968608598 8.696642834966418E-5 68-69 1.7393285669932835E-4 0.0 0.0 1.6084006091128642 8.696642834966418E-5 70-71 1.7393285669932835E-4 0.0 0.0 1.8598205534717431 8.696642834966418E-5 72-73 1.7393285669932835E-4 0.0 0.0 2.1277641192170584 8.696642834966418E-5 74-75 1.7393285669932835E-4 0.0 0.0 2.4035346635138435 8.696642834966418E-5 76-77 1.7393285669932835E-4 0.0 0.0 2.7134830141520467 8.696642834966418E-5 78-79 1.7393285669932835E-4 0.0 0.0 3.073132678592083 8.696642834966418E-5 80-81 1.7393285669932835E-4 0.0 0.0 3.450219111916227 8.696642834966418E-5 82-83 1.7393285669932835E-4 0.0 0.0 3.846481642691472 8.696642834966418E-5 84-85 1.7393285669932835E-4 0.0 0.0 4.268703652329091 8.696642834966418E-5 86-87 1.7393285669932835E-4 0.0 0.0 4.691577910179333 8.696642834966418E-5 88-89 1.7393285669932835E-4 0.0 0.0 5.138280969397384 8.696642834966418E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 4705 0.0 26.468071 1 TACATGG 4940 0.0 25.01653 2 GAGTACT 4415 0.0 24.165216 12-13 ACATGGG 4855 0.0 24.08598 3 GTATCAA 8290 0.0 22.475645 1 GTACTTT 4640 0.0 22.12091 14-15 GGTATCA 6260 0.0 22.095268 1 CATGGGG 2350 0.0 20.026484 4 ATCAACG 9290 0.0 19.853382 3 TCAACGC 9290 0.0 19.803076 4 TATCAAC 9490 0.0 19.633627 2 CAACGCA 9425 0.0 19.570713 5 TCTATAC 365 5.456968E-12 19.535156 3 AACGCAG 9500 0.0 19.466248 6 CATGGGA 2595 0.0 19.051683 4 ACACCGT 150 7.6778117E-4 19.015875 6 GTATTAC 200 1.2774315E-5 19.012562 1 AGTACTT 4620 0.0 18.978394 12-13 GTATAGG 390 1.4551915E-11 18.281311 1 ACTTTTT 5735 0.0 18.020805 16-17 >>END_MODULE