FastQCFastQC Report
Thu 26 May 2016
SRR936400_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936400_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1149869
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41950.3648241669268412No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39470.3432564926961245No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37650.32742860273648566No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23400.20350144233821418No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG1151.2836911E-424.7770964
GTAAGGT4250.024.5827664
GCGGTCG1201.7133997E-423.7447159
TAAGGTG5200.022.8314575
GTACATG45050.021.4029581
GAGTACT51800.020.99527712-13
ACGCGAA600.00475847219.79630378-79
TAACGCA1456.155256E-419.65084
ACATGGG45500.019.6220073
AGTACTT53550.019.59966712-13
GTACTTT55550.019.4924614-15
TACATGG49950.019.0156142
GTATAGG3759.094947E-1218.9990791
GTGTAAG5150.018.4457071
CTACACT4201.8189894E-1218.0912134
GTGTAGC6050.018.0569741
TACTCCC3451.0859367E-917.894575
GCACCGT2152.3990095E-517.6704866
GGTATCA88200.017.6635421
ATACGTC2704.884787E-717.588685
Produced by FastQC (version 0.11.5)