##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936400_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1149869 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.307888985614884 31.0 31.0 34.0 30.0 34.0 2 31.504113077228798 31.0 31.0 34.0 30.0 34.0 3 31.546401372678105 31.0 31.0 34.0 30.0 34.0 4 35.356893698325635 37.0 35.0 37.0 33.0 37.0 5 35.14659930826903 37.0 35.0 37.0 32.0 37.0 6 35.24801955701041 37.0 35.0 37.0 32.0 37.0 7 35.172483996003024 37.0 35.0 37.0 32.0 37.0 8 35.1322568049056 37.0 35.0 37.0 32.0 37.0 9 36.65408581325351 38.0 35.0 39.0 32.0 39.0 10-11 36.50889144763447 38.0 35.0 39.0 32.0 39.0 12-13 36.37736646522343 38.0 35.0 39.0 32.0 39.0 14-15 37.30668537024653 39.0 36.0 40.0 32.0 41.0 16-17 37.177299327140744 39.0 36.0 40.0 32.0 41.0 18-19 37.33439330915087 39.0 36.0 40.0 32.0 41.0 20-21 37.3321282685245 39.0 36.0 40.0 32.0 41.0 22-23 37.233986219299766 39.0 36.0 40.0 31.0 41.0 24-25 37.05173763272164 39.0 36.0 40.0 31.0 41.0 26-27 36.75618961812172 39.0 35.5 40.0 30.5 41.0 28-29 36.626186548206796 38.5 35.0 40.0 30.0 41.0 30-31 36.57166468528154 38.0 35.0 40.0 30.0 41.0 32-33 36.39084539195335 38.0 35.0 40.0 30.0 41.0 34-35 36.16440655413791 38.0 35.0 40.0 30.0 41.0 36-37 35.88696973307394 38.0 35.0 40.0 29.5 41.0 38-39 35.659409463164934 38.0 34.0 40.0 28.5 41.0 40-41 35.43376245467962 38.0 34.0 40.0 27.5 41.0 42-43 35.10882978843677 38.0 34.0 40.0 26.5 41.0 44-45 34.76544023710527 38.0 33.0 40.0 25.5 41.0 46-47 34.32827304675576 38.0 33.0 40.0 24.0 41.0 48-49 33.873070758495096 37.5 33.0 40.0 23.0 41.0 50-51 33.50154321927106 37.0 32.0 40.0 22.5 41.0 52-53 33.09589875020546 37.0 31.5 39.5 20.0 40.5 54-55 32.83990524137967 36.5 31.0 39.0 20.0 40.0 56-57 32.45158144101632 36.0 31.0 39.0 18.5 40.0 58-59 32.00364345851571 36.0 30.5 39.0 16.0 40.0 60-61 31.50679686120767 35.0 29.5 38.5 13.0 40.0 62-63 31.080588745326644 35.0 29.0 38.0 10.5 40.0 64-65 30.55621162062809 34.0 28.0 38.0 8.0 40.0 66-67 30.042136104199695 34.0 28.0 37.5 7.5 39.5 68-69 29.593130173958947 34.0 28.0 37.0 7.0 39.0 70-71 29.137119532746773 33.5 26.5 36.0 2.0 39.0 72-73 28.59122343501738 33.0 26.0 36.0 2.0 39.0 74-75 27.97445926449013 32.5 26.0 35.0 2.0 38.0 76-77 26.756054385325633 30.5 25.0 34.0 2.0 36.0 78-79 27.493895826394137 32.0 26.0 35.0 2.0 37.0 80-81 27.234807182383385 32.0 25.5 35.0 2.0 36.5 82-83 26.783883642397527 32.0 25.0 35.0 2.0 36.0 84-85 26.302064409076166 32.0 24.0 34.0 2.0 35.5 86-87 25.750093271494407 31.0 22.5 34.0 2.0 35.0 88-89 25.081837583237743 31.0 20.0 34.0 2.0 35.0 90-91 24.339115151378113 31.0 18.0 34.0 2.0 35.0 92-93 23.37063569850131 30.0 8.5 34.0 2.0 35.0 94-95 22.53222410552854 29.0 2.0 34.0 2.0 35.0 96-97 21.509698061257414 29.0 2.0 34.0 2.0 35.0 98-99 20.259324757863723 29.0 2.0 33.5 2.0 34.5 100-101 17.11701811249803 21.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3943407274129207 1101 2 0.3971607081591628 1101 3 0.4447895417928258 1101 4 0.4619291262608911 1101 5 0.2819355082421211 1101 6 0.33738796131730453 1101 7 0.45595732324803606 1101 8 0.3402325045421577 1101 9 0.2560065013756372 1101 10-11 0.4444335925259679 1101 12-13 0.49866673757659896 1101 14-15 0.730767213553797 1101 16-17 0.7148515541466125 1101 18-19 0.6192000163845961 1101 20-21 0.5258272948861347 1101 22-23 0.4874587086183979 1101 24-25 0.538735009560618 1101 26-27 0.6149601825509521 1101 28-29 0.630397175086479 1101 30-31 0.7858470884303586 1101 32-33 0.5798596687427846 1101 34-35 0.7421041287041135 1101 36-37 0.7352853000538104 1101 38-39 0.7632468337488163 1101 40-41 0.843326435079149 1101 42-43 0.9916404348888719 1101 44-45 1.0756924759760906 1101 46-47 0.955173357831228 1101 48-49 0.915044992370305 1101 50-51 1.0113463379202798 1101 52-53 1.2262294120191726 1101 54-55 1.0743618243373732 1101 56-57 1.0506750127836995 1101 58-59 0.8019556849698581 1101 60-61 1.1340458411668415 1101 62-63 1.0076498201237314 1101 64-65 1.0870378008748034 1101 66-67 0.8616940413526812 1101 68-69 0.7269995985763771 1101 70-71 0.7558185869818423 1101 72-73 0.9980158058783459 1101 74-75 1.0890706238574772 1101 76-77 0.8396597387222968 1101 78-79 0.9205840003639594 1101 80-81 1.0427799920709226 1101 82-83 1.0940144585579183 1101 84-85 1.0538134060634512 1101 86-87 1.0027759940826257 1101 88-89 1.115125104602555 1101 90-91 1.2287622267328757 1101 92-93 1.1044798648629524 1101 94-95 1.1542380611857403 1101 96-97 1.5476177137551907 1101 98-99 1.6184281027269556 1101 100-101 1.984541171932129 1102 1 0.38999299432485657 1102 2 0.43408208212944643 1102 3 0.43529169279907975 1102 4 0.4174678671000649 1102 5 0.38950159587056277 1102 6 0.4036381518197203 1102 7 0.3550220566382336 1102 8 0.3840384353837649 1102 9 0.45647680839869054 1102 10-11 0.38334033591052474 1102 12-13 0.5818606810702391 1102 14-15 0.7382804952487163 1102 16-17 0.7014705305938307 1102 18-19 0.6394938045352134 1102 20-21 0.5438636628004545 1102 22-23 0.612572007425122 1102 24-25 0.49101943435025674 1102 26-27 0.5425906533125087 1102 28-29 0.6541019591703758 1102 30-31 0.6972431436266007 1102 32-33 0.775582521813952 1102 34-35 0.786562045717254 1102 36-37 0.7468828199128481 1102 38-39 0.8322125860930001 1102 40-41 0.8395169214552212 1102 42-43 0.9740013814920445 1102 44-45 0.9537774788235964 1102 46-47 1.0091620105713872 1102 48-49 1.2980885392178507 1102 50-51 1.0554959752304214 1102 52-53 1.1450009187395622 1102 54-55 1.067062841152385 1102 56-57 0.9375830181632239 1102 58-59 1.2777378143724683 1102 60-61 1.3786736729685778 1102 62-63 1.4036463282478238 1102 64-65 1.2489633859511784 1102 66-67 1.1512443486965829 1102 68-69 1.396809176369544 1102 70-71 1.4026369960660645 1102 72-73 1.3517400278932072 1102 74-75 1.3482376002298295 1102 76-77 1.1895097431339288 1102 78-79 1.4725104142929588 1102 80-81 1.6411238910471546 1102 82-83 1.5781427328600124 1102 84-85 1.5008002593912941 1102 86-87 1.7131037651909615 1102 88-89 1.6028641015839291 1102 90-91 1.643718872393027 1102 92-93 1.8777556508692435 1102 94-95 1.522771606990542 1102 96-97 1.8519848312998626 1102 98-99 2.139026427375164 1102 100-101 2.4441227083040538 1103 1 0.3054820097109001 1103 2 0.3435930055614129 1103 3 0.22872662086129836 1103 4 0.3170605786123062 1103 5 0.36317804594483505 1103 6 0.23680894500747485 1103 7 0.2090546837682865 1103 8 0.20427872151967819 1103 9 0.28350340053754053 1103 10-11 0.37153955424945906 1103 12-13 0.4056860786919714 1103 14-15 0.599129101645417 1103 16-17 0.4243314247808101 1103 18-19 0.4914318663388073 1103 20-21 0.30375266032135784 1103 22-23 0.08906114639877671 1103 24-25 0.5495533120401248 1103 26-27 0.4041821619868955 1103 28-29 0.5025391876753105 1103 30-31 0.5410886640038797 1103 32-33 0.6243921956650098 1103 34-35 0.6840329659188171 1103 36-37 0.6538710570640163 1103 38-39 0.7589791241959247 1103 40-41 0.9502353631744782 1103 42-43 1.0524288881792359 1103 44-45 1.1032329897299036 1103 46-47 1.1943589676240833 1103 48-49 1.291691044742585 1103 50-51 1.4201667733127294 1103 52-53 1.3287018232540575 1103 54-55 1.225326416823492 1103 56-57 1.1275715954862378 1103 58-59 1.4013904340627938 1103 60-61 1.181768319055255 1103 62-63 1.2061089644083367 1103 64-65 1.142366315834174 1103 66-67 0.9404618494368862 1103 68-69 1.193531412595643 1103 70-71 1.0776119726333633 1103 72-73 1.1905952394972275 1103 74-75 1.0768872281209845 1103 76-77 1.2591603878697093 1103 78-79 0.9328588273545826 1103 80-81 1.2234301827579763 1103 82-83 1.360079118277163 1103 84-85 1.64118676435805 1103 86-87 1.7558571259175118 1103 88-89 1.3980034068502292 1103 90-91 1.4463390521701776 1103 92-93 1.4279565419237024 1103 94-95 1.4207581383879138 1103 96-97 1.563933918097522 1103 98-99 1.764602914648087 1103 100-101 1.82515418154183 1104 1 0.2376565526106802 1104 2 0.20501209199965942 1104 3 0.3061244736950606 1104 4 0.3453858412017965 1104 5 0.277102691906272 1104 6 0.18765724405466244 1104 7 0.29579340650049346 1104 8 0.3119048486658329 1104 9 0.2598275250940816 1104 10-11 0.15951850063997597 1104 12-13 0.26121867520704 1104 14-15 0.42079106521674703 1104 16-17 0.48322462967899327 1104 18-19 0.2712224915021082 1104 20-21 0.4023365786384616 1104 22-23 0.38227192345464545 1104 24-25 0.41622309958795256 1104 26-27 0.45737903297692384 1104 28-29 0.5458076647433643 1104 30-31 0.5914974268575435 1104 32-33 0.51347861939648 1104 34-35 0.5637106587312033 1104 36-37 0.592441591881439 1104 38-39 0.552863430953856 1104 40-41 0.5987831373330081 1104 42-43 0.7352250670594387 1104 44-45 0.7945723993462792 1104 46-47 0.7718293990895404 1104 48-49 1.0581613136318992 1104 50-51 0.9236015570574381 1104 52-53 0.992472965409469 1104 54-55 0.8386060564850979 1104 56-57 0.7215155618794284 1104 58-59 0.9553501064718724 1104 60-61 1.0770373535271993 1104 62-63 1.0561448182523918 1104 64-65 1.2117456814388703 1104 66-67 1.3923316927251044 1104 68-69 1.1657062167784922 1104 70-71 1.1972905073578843 1104 72-73 1.4932597462238135 1104 74-75 1.315093200471427 1104 76-77 1.1664988858984735 1104 78-79 1.1146875330743562 1104 80-81 1.3256121867410933 1104 82-83 1.4382121926499387 1104 84-85 1.7502938584838255 1104 86-87 1.8682465886614459 1104 88-89 1.814423669062311 1104 90-91 2.030970091438121 1104 92-93 1.877066945635086 1104 94-95 1.8717867953348524 1104 96-97 2.0816017344097126 1104 98-99 1.98435685857093 1104 100-101 2.268556275870825 1105 1 0.0481917170896935 1105 2 0.23695586124289392 1105 3 0.13847538926789937 1105 4 0.24095584606325104 1105 5 0.2259967123243527 1105 6 0.3702046431796049 1105 7 0.08694354424154227 1105 8 0.27171687264898026 1105 9 0.3031146746646556 1105 10-11 0.3176472480066934 1105 12-13 0.25708137979300005 1105 14-15 0.5130269265034144 1105 16-17 0.35109956891088956 1105 18-19 0.3904799052114001 1105 20-21 0.32354059743605745 1105 22-23 0.47012741689168536 1105 24-25 0.3664185703382401 1105 26-27 0.4170036886243125 1105 28-29 0.27762280815609586 1105 30-31 0.41456094978738633 1105 32-33 0.35449405449614346 1105 34-35 0.6054482215249166 1105 36-37 0.568022940123214 1105 38-39 0.5400920000674532 1105 40-41 0.6038623384349293 1105 42-43 0.675682448359062 1105 44-45 0.6998095975313419 1105 46-47 0.6764234681221097 1105 48-49 0.7595366984965892 1105 50-51 0.9551250135474731 1105 52-53 1.0763025716321195 1105 54-55 1.0163021349164723 1105 56-57 1.0106249039633681 1105 58-59 0.9070343958901255 1105 60-61 0.9745367458461089 1105 62-63 0.9618732253176958 1105 64-65 1.000606887078149 1105 66-67 0.8312546792750943 1105 68-69 0.8673905061967382 1105 70-71 1.1600092725499103 1105 72-73 1.281867751409358 1105 74-75 1.0729981186370416 1105 76-77 0.958363048919054 1105 78-79 0.846735620904532 1105 80-81 1.1091460548337828 1105 82-83 1.2914926577285328 1105 84-85 1.5534862098045217 1105 86-87 1.7644749147026069 1105 88-89 1.5769672195403501 1105 90-91 1.813703846097006 1105 92-93 1.8131895351667673 1105 94-95 1.903922996922926 1105 96-97 2.1896069199550077 1105 98-99 1.848150206488615 1105 100-101 2.0637930689351585 1106 1 0.11947143344516675 1106 2 0.17092943536798444 1106 3 0.09083101624473855 1106 4 0.22542809379307016 1106 5 0.1863553491981449 1106 6 0.009159851831782362 1106 7 -0.18406069002026726 1106 8 0.0592079449885361 1106 9 0.06920231878260097 1106 10-11 0.08704705414891833 1106 12-13 0.2039433071473553 1106 14-15 0.2776950726109533 1106 16-17 0.08625988418483388 1106 18-19 0.26360382113833936 1106 20-21 0.1075141715611494 1106 22-23 0.07780074104228163 1106 24-25 -0.06757732293795726 1106 26-27 -0.030163054565157665 1106 28-29 -0.22352949773105735 1106 30-31 -0.07627671983816242 1106 32-33 -0.011544487489366873 1106 34-35 0.11744572177998691 1106 36-37 -0.18278947697708503 1106 38-39 0.19574320912776244 1106 40-41 0.1879806681971985 1106 42-43 0.2045506760909248 1106 44-45 0.12232707023820666 1106 46-47 0.2000231008126505 1106 48-49 0.21691222140241706 1106 50-51 0.5423412414833919 1106 52-53 0.15927497240755173 1106 54-55 -0.028072163624493385 1106 56-57 -0.15074957313080262 1106 58-59 0.08533954332405713 1106 60-61 0.1599432094722708 1106 62-63 0.06985710451369442 1106 64-65 0.12529952048290482 1106 66-67 0.23137902883126316 1106 68-69 0.1156732069415618 1106 70-71 0.5447008856506166 1106 72-73 0.5099924325643386 1106 74-75 0.37497304466500125 1106 76-77 0.27116977782426943 1106 78-79 0.2183169646449663 1106 80-81 0.2245360660571265 1106 82-83 0.21513501565119242 1106 84-85 0.5319833019379736 1106 86-87 0.9577230526477152 1106 88-89 0.9360995784833186 1106 90-91 0.8074340777060272 1106 92-93 0.8192386628068 1106 94-95 0.6584722776085705 1106 96-97 1.3267179290357127 1106 98-99 1.3762232347510341 1106 100-101 1.4557715660272912 1107 1 0.18818252019982395 1107 2 0.19315089645093408 1107 3 0.17966586424522646 1107 4 0.27859367203057417 1107 5 0.1467213049607281 1107 6 0.14593810151590958 1107 7 0.03997380481159496 1107 8 0.13500676065582695 1107 9 0.07967483404344478 1107 10-11 0.06250297657360449 1107 12-13 0.04198657030364927 1107 14-15 0.23540122718311807 1107 16-17 0.2376424936747199 1107 18-19 0.10256311541000684 1107 20-21 0.08136941809416243 1107 22-23 0.015740869827048698 1107 24-25 0.1886316706836979 1107 26-27 0.17797276312676047 1107 28-29 0.1459324529166679 1107 30-31 0.12849181770883433 1107 32-33 0.25936324720792925 1107 34-35 0.4486151361594324 1107 36-37 0.23321347624330002 1107 38-39 0.19480929123945856 1107 40-41 0.22373176043205945 1107 42-43 0.1371784333179491 1107 44-45 0.4112441212802054 1107 46-47 0.35654111697924407 1107 48-49 0.5515729816283184 1107 50-51 0.4979131615333259 1107 52-53 0.5778992892850567 1107 54-55 0.4259184895852286 1107 56-57 0.3954093375266723 1107 58-59 0.3700650003881911 1107 60-61 0.5253326844383999 1107 62-63 0.4784046985524526 1107 64-65 0.4610277785395631 1107 66-67 0.5614562083535724 1107 68-69 0.5491465899011772 1107 70-71 0.46803056551543776 1107 72-73 0.7962515852641019 1107 74-75 0.8162682753663155 1107 76-77 0.7060794305925313 1107 78-79 0.9862395111125188 1107 80-81 1.1361259337711225 1107 82-83 1.007069603342046 1107 84-85 1.1163498818347648 1107 86-87 1.121799562367464 1107 88-89 0.6981187287474349 1107 90-91 0.9521106696288193 1107 92-93 1.3459512261716462 1107 94-95 1.4471910518698827 1107 96-97 1.4115895248403127 1107 98-99 1.0958054454039399 1107 100-101 1.2627028808192726 1108 1 -0.06568081478588894 1108 2 0.11111763030932309 1108 3 -0.06051939887445812 1108 4 0.15825942251522918 1108 5 0.19769929050222146 1108 6 0.08259449469298374 1108 7 0.08059171153799127 1108 8 -0.05902890578434494 1108 9 -0.007045026385320341 1108 10-11 0.07140455639204646 1108 12-13 0.19005640583328187 1108 14-15 0.10664126044245847 1108 16-17 0.259350108724 1108 18-19 0.15338172384939952 1108 20-21 -0.018143999642866504 1108 22-23 0.1665184145575651 1108 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-1.4067810170648585 2316 98-99 -2.4423329230327653 2316 100-101 -2.7685960943159778 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 16.0 4 25.0 5 60.0 6 147.0 7 395.0 8 886.0 9 1614.0 10 2493.0 11 3621.0 12 4964.0 13 6935.0 14 8983.0 15 10852.0 16 13470.0 17 15857.0 18 14508.0 19 11978.0 20 12071.0 21 12850.0 22 13877.0 23 15452.0 24 18372.0 25 21683.0 26 26109.0 27 31227.0 28 37387.0 29 44170.0 30 51845.0 31 61353.0 32 72232.0 33 86528.0 34 104287.0 35 126856.0 36 146814.0 37 128012.0 38 41048.0 39 890.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.669963833225765 21.44260025015787 15.713063007426452 22.17437290918991 2 16.73720337184585 22.376921712085 37.64677027125755 23.239104644811594 3 20.22522334474409 25.3237976955832 30.644412206729566 23.80656675294314 4 14.026119497090539 18.512717535649713 36.680178350751255 30.78098461650849 5 13.398791146671304 38.45988607209636 34.110188285428535 14.031134495803801 6 31.543506260278342 37.749691486595424 16.421957631695435 14.28484462143079 7 27.871870621783874 33.742104535386204 20.627306240971798 17.758718601858124 8 26.34013091926124 35.27001771506145 19.33672444426278 19.053126921414528 9 26.13549891335448 17.08412001714978 20.293094256824038 36.487286812671705 10-11 25.09590222886259 27.07821499666484 27.15761534574808 20.66826742872449 12-13 26.160956046407367 24.43883410204977 27.672965546690232 21.727244304852633 14-15 22.66974238336156 26.1030944869735 26.287507038854475 24.939656090810463 16-17 21.820268221858317 29.271725735714245 27.01399028932861 21.89401575309883 18-19 21.6398800374133 27.71380130685645 29.665171845057458 20.98114681067279 20-21 22.462123946293012 26.510715568469106 29.773956859433554 21.253203625804332 22-23 22.108624642132835 26.5476124215997 29.60531121833115 21.738451717936318 24-25 22.426337261027125 26.796095903098525 29.594979949889943 21.182586885984403 26-27 22.135660583290935 26.93530923464263 29.69574424601363 21.233285936052805 28-29 21.67180723918425 27.439394802492 29.703319463281613 21.185478495042133 30-31 21.930001548022616 26.89293597274089 29.61506417336462 21.561998305871878 32-33 21.844911612082036 27.149969624874814 29.425734014791406 21.579384748251744 34-35 22.498740059076987 26.784495964927768 29.382518064226332 21.334245911768914 36-37 22.62627466105789 26.594872700218158 29.25618627238253 21.522666366341426 38-39 22.15822420429582 27.083641341308784 29.42948806030637 21.32864639408903 40-41 22.14122248104454 27.031311895772248 29.406718084229777 21.420747538953435 42-43 22.109079302571523 27.257606381498384 28.808234999443393 21.825079316486697 44-45 22.42770348776534 27.162546701824937 28.57008056717062 21.839669243239108 46-47 22.654291891403 27.5107708202521 28.08701187790577 21.74792541043913 48-49 22.81006900810095 27.670161279802414 27.82631004509225 21.693459667004387 50-51 22.405199905728228 27.72590221303855 27.99115028085857 21.877747600374654 52-53 22.830188104935935 27.847348837492454 27.530789524147863 21.79167353342375 54-55 22.281264458583657 27.60641517572941 27.883541652896586 22.228778712790344 56-57 22.73322692737576 27.847323397278384 27.556200176570105 21.86324949877575 58-59 22.906974721202523 27.383131230536367 27.63161328485186 22.078280763409243 60-61 22.550837340083373 27.601930198826135 28.08221829339631 21.765014167694176 62-63 22.466308191975894 27.612017542181082 28.0584002936454 21.863273972197625 64-65 22.262606581582617 27.753854985984834 27.841925459760493 22.14161297267206 66-67 22.35614982517034 27.769368347678164 27.53924807374285 22.335233753408644 68-69 22.267184119840415 27.653915142027852 27.674743719357746 22.404157018773986 70-71 22.469675853957085 27.600919236346797 27.631096626350427 22.298308283345687 72-73 22.563580096332757 27.436876477614614 27.654683992126056 22.344859433926576 74-75 22.355442786978273 28.011410208745076 27.237568460165367 22.395578544111284 76-77 22.422357304464253 27.591057669603757 27.42520771467546 22.561377311256532 78-79 22.334032899011184 27.740069457913428 27.375428458610095 22.550469184465292 80-81 22.293796947356334 28.020722071650496 27.24555794073543 22.43992304025774 82-83 22.35107618306202 27.811579895012894 27.329048027033963 22.50829589489112 84-85 22.014534063095773 27.769177468765943 27.23494821473389 22.981340253404394 86-87 22.162459762536997 27.822014947766316 27.157428177052665 22.85809711264402 88-89 22.082538185579764 27.67253383120784 27.006197476204292 23.238730507008103 90-91 22.07506959226048 27.6688561203125 27.280514123563837 22.975560163863182 92-93 22.125832224570473 27.72887235881378 27.140731474671277 23.00456394194447 94-95 21.862948847656472 27.63271328120175 27.118762760683307 23.385575110458475 96-97 21.968857863537373 27.584483225167077 27.017882888095563 23.428776023199987 98-99 21.901118583999953 27.72240182969035 26.62635396259611 23.75012562371358 100-101 21.76607921503723 27.651951225242584 26.72208778098871 23.859881778731484 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 305.0 1 203.0 2 135.0 3 192.0 4 198.0 5 205.5 6 302.5 7 516.5 8 792.5 9 999.0 10 982.5 11 901.0 12 947.5 13 1057.0 14 1241.0 15 1388.5 16 1483.5 17 1598.5 18 1768.0 19 1913.0 20 2085.0 21 2448.5 22 2825.0 23 3287.5 24 3877.0 25 4817.0 26 6385.0 27 8152.5 28 9752.0 29 11780.0 30 14358.0 31 16889.0 32 20062.5 33 22884.5 34 25122.5 35 27528.0 36 30409.5 37 33014.0 38 34084.0 39 34030.5 40 34553.0 41 36511.0 42 38059.0 43 39218.5 44 40117.0 45 40986.0 46 41013.5 47 42655.5 48 49372.0 49 49097.5 50 42814.0 51 39440.0 52 36853.0 53 35713.5 54 34409.0 55 31669.5 56 28599.5 57 25899.5 58 23564.0 59 21154.0 60 17878.0 61 14809.5 62 12487.0 63 9887.0 64 7474.5 65 5786.5 66 4465.0 67 3627.0 68 2678.0 69 1757.5 70 1333.5 71 1116.0 72 961.0 73 844.5 74 684.5 75 532.0 76 361.0 77 220.5 78 156.5 79 106.0 80 78.0 81 59.0 82 35.0 83 24.0 84 18.0 85 12.5 86 5.5 87 0.5 88 0.0 89 0.5 90 0.5 91 0.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016958453528184515 2 0.009827206403512052 3 0.006696414982924141 4 0.0 5 0.0016523621386436193 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 7.392146409721456E-4 14-15 1.3044964252449627E-4 16-17 0.0 18-19 9.131474976714739E-4 20-21 0.0 22-23 8.696642834966418E-5 24-25 0.0 26-27 0.0012175299968952985 28-29 0.002565509636315093 30-31 0.0013044964252449625 32-33 0.006913831053798302 34-35 0.0011740467827204663 36-37 3.9134892757348885E-4 38-39 6.087649984476492E-4 40-41 2.1741607087416044E-4 42-43 0.003913489275734888 44-45 0.0018262949953429478 46-47 2.6089928504899253E-4 48-49 3.478657133986567E-4 50-51 1.7393285669932835E-4 52-53 4.78315355923153E-4 54-55 0.002869892135538918 56-57 0.016001822816338208 58-59 0.02513329779305295 60-61 0.02143722458819222 62-63 0.015914856387988546 64-65 0.019263063879450613 66-67 0.003087308206413078 68-69 5.652817842728172E-4 70-71 1.3044964252449627E-4 72-73 4.348321417483209E-5 74-75 0.00186977820951778 76-77 0.003000341778063414 78-79 0.00939237426176373 80-81 0.015393057817890559 82-83 0.01774115138333149 84-85 0.03152533027675326 86-87 0.011479568542155672 88-89 0.03909140954317405 90-91 0.0430918652472586 92-93 0.027350941715969385 94-95 0.029742518495585152 96-97 0.04852726701911261 98-99 0.05248423950902233 100-101 0.05809357413757567 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1149869.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.595696446217126 #Duplication Level Percentage of deduplicated Percentage of total 1 78.4063539742761 45.15868562957056 2 12.770784806745342 14.710844902185327 3 3.926894160408566 6.785166121179433 4 1.6454874205104215 3.7909197591114827 5 0.8779442769391866 2.5282906035641495 6 0.4977806681802369 1.7202014556782435 7 0.3365837265138881 1.3570041898721326 8 0.22886878224389276 1.0545485526507694 9 0.1694405998976512 0.8783144421633042 >10 0.9953975215847738 10.968586229545956 >50 0.09512541766479679 3.810856567784566 >100 0.04541569857624398 4.652641671001655 >500 0.0030176511221604273 1.164224205106989 >1k 9.052953366481283E-4 1.4197156705854206 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4195 0.3648241669268412 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3947 0.3432564926961245 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3765 0.32742860273648566 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2340 0.20350144233821418 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.3044964252449627E-4 0.0 18-19 0.0 0.0 0.0 1.7393285669932835E-4 0.0 20-21 0.0 0.0 0.0 1.7393285669932835E-4 0.0 22-23 0.0 0.0 0.0 2.1741607087416044E-4 8.696642834966418E-5 24-25 0.0 0.0 0.0 3.913489275734888E-4 8.696642834966418E-5 26-27 0.0 0.0 0.0 6.522482126224813E-4 8.696642834966418E-5 28-29 0.0 0.0 0.0 0.0016088789244687873 8.696642834966418E-5 30-31 0.0 0.0 0.0 0.005261468915154683 8.696642834966418E-5 32-33 0.0 0.0 0.0 0.011088219614582184 8.696642834966418E-5 34-35 0.0 0.0 0.0 0.020958909232269066 8.696642834966418E-5 36-37 0.0 0.0 0.0 0.034960504196565 8.696642834966418E-5 38-39 0.0 0.0 0.0 0.05857188949349883 8.696642834966418E-5 40-41 0.0 0.0 0.0 0.08840137441743365 8.696642834966418E-5 42-43 0.0 0.0 0.0 0.1214051339761312 8.696642834966418E-5 44-45 0.0 0.0 0.0 0.16254025458552235 8.696642834966418E-5 46-47 0.0 0.0 0.0 0.22298192228853897 8.696642834966418E-5 48-49 0.0 0.0 0.0 0.28711966319641624 8.696642834966418E-5 50-51 0.0 0.0 0.0 0.35404032981148287 8.696642834966418E-5 52-53 0.0 0.0 0.0 0.42626594855587896 8.696642834966418E-5 54-55 0.0 0.0 0.0 0.5074491094202904 8.696642834966418E-5 56-57 0.0 0.0 0.0 0.6125480380808597 8.696642834966418E-5 58-59 0.0 0.0 0.0 0.7325617092033962 8.696642834966418E-5 60-61 0.0 0.0 0.0 0.864141915296438 8.696642834966418E-5 62-63 0.0 0.0 0.0 1.0058971935063907 8.696642834966418E-5 64-65 0.0 0.0 0.0 1.1568709131214079 1.7393285669932835E-4 66-67 0.0 0.0 0.0 1.3323256823168552 1.7393285669932835E-4 68-69 0.0 0.0 0.0 1.545002082845959 1.7393285669932835E-4 70-71 0.0 0.0 0.0 1.7840727943791856 1.7393285669932835E-4 72-73 0.0 0.0 0.0 2.039667127298849 1.7393285669932835E-4 74-75 0.0 0.0 0.0 2.301436076631338 1.7393285669932835E-4 76-77 0.0 0.0 0.0 2.5975132819477693 1.7393285669932835E-4 78-79 0.0 0.0 0.0 2.9380738153650547 1.7393285669932835E-4 80-81 0.0 0.0 0.0 3.2983322448035386 1.7393285669932835E-4 82-83 0.0 0.0 0.0 3.6735923831323394 1.7393285669932835E-4 84-85 0.0 0.0 0.0 4.071028960690305 1.7393285669932835E-4 86-87 0.0 0.0 0.0 4.4637258679032135 1.7393285669932835E-4 88-89 0.0 0.0 0.0 4.8775121339909155 1.7393285669932835E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCGCG 115 1.2836911E-4 24.777096 4 GTAAGGT 425 0.0 24.582766 4 GCGGTCG 120 1.7133997E-4 23.744715 9 TAAGGTG 520 0.0 22.831457 5 GTACATG 4505 0.0 21.402958 1 GAGTACT 5180 0.0 20.995277 12-13 ACGCGAA 60 0.004758472 19.796303 78-79 TAACGCA 145 6.155256E-4 19.6508 4 ACATGGG 4550 0.0 19.622007 3 AGTACTT 5355 0.0 19.599667 12-13 GTACTTT 5555 0.0 19.49246 14-15 TACATGG 4995 0.0 19.015614 2 GTATAGG 375 9.094947E-12 18.999079 1 GTGTAAG 515 0.0 18.445707 1 CTACACT 420 1.8189894E-12 18.091213 4 GTGTAGC 605 0.0 18.056974 1 TACTCCC 345 1.0859367E-9 17.89457 5 GCACCGT 215 2.3990095E-5 17.670486 6 GGTATCA 8820 0.0 17.663542 1 ATACGTC 270 4.884787E-7 17.58868 5 >>END_MODULE