FastQCFastQC Report
Thu 26 May 2016
SRR936399_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936399_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629114
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41750.6636317106279624No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT39480.6275492200141787No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33120.5264546648143261No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC25560.40628566523714305No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT24650.3918208782510006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24290.38609854493780144No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15820.25146475837447585No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT15400.24478870284241014No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT11980.19042653636701773No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC11460.18216094380350778No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC9160.1456015920802907No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC8220.130659943984715No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG8130.12922936065641522No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA7840.12461970326522696No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7010.11142654590423992No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA6840.10872433295078475No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA6820.10840642554449591No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA6510.10347886074701883No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT608.277883E-539.5902946
TACCTGG4850.034.2843782
GTACCTG5950.029.545361
ATTCGCG1007.1304385E-1026.14103180-81
GTACATG29100.026.1235081
GTAGTAC1109.463736E-525.9115853
GTAAGCG659.99604E-625.58549194-95
TACATGG29950.025.2214642
ATGGGAG7400.025.0361945
GAGTACT28750.023.95882812-13
ACATGGG29850.023.8716433
GTACTTT30950.022.1781514-15
CATGGGG13100.021.757824
GTGTAAG1754.0354716E-621.7198331
TCCGCGT550.002865304721.60673764-65
CATGGGA15800.021.3469924
ACAGTCC2457.494236E-921.3285858
GTAGCCC2751.2532837E-920.7292693
AGTACTT30300.020.69506312-13
CCCGCGA1401.5734258E-920.36072320-21
Produced by FastQC (version 0.11.5)