##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936399_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 629114 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.844290541936754 33.0 31.0 34.0 30.0 34.0 2 31.918747635563665 33.0 31.0 34.0 30.0 34.0 3 31.95536897923111 33.0 31.0 34.0 30.0 34.0 4 35.57547121825297 37.0 35.0 37.0 33.0 37.0 5 35.587569184599296 37.0 35.0 37.0 33.0 37.0 6 35.56553979088051 37.0 35.0 37.0 33.0 37.0 7 35.52587448379785 37.0 35.0 37.0 33.0 37.0 8 35.50595917433088 37.0 35.0 37.0 33.0 37.0 9 37.08743248441459 39.0 37.0 39.0 33.0 39.0 10-11 36.98526419695 39.0 37.0 39.0 33.0 39.0 12-13 36.905533019452754 39.0 37.0 39.0 33.0 39.0 14-15 38.08072304860487 40.0 38.0 41.0 33.0 41.0 16-17 38.015690320037386 40.0 38.0 41.0 33.0 41.0 18-19 38.00638358071828 40.0 38.0 41.0 33.0 41.0 20-21 37.87657006520281 40.0 38.0 41.0 33.0 41.0 22-23 37.98086435844696 40.0 38.0 41.0 33.0 41.0 24-25 38.011278560006616 40.0 38.0 41.0 33.0 41.0 26-27 37.907262435742965 40.0 38.0 41.0 33.0 41.0 28-29 37.77885009712071 40.0 37.5 41.0 33.0 41.0 30-31 37.57884580537073 40.0 37.0 41.0 32.5 41.0 32-33 37.3663382153314 40.0 36.5 41.0 32.0 41.0 34-35 37.13885162307626 39.0 36.0 41.0 31.5 41.0 36-37 36.93254879083918 39.0 36.0 41.0 31.0 41.0 38-39 36.73002667243139 39.0 36.0 41.0 30.5 41.0 40-41 36.54763763006387 39.0 35.0 40.5 30.0 41.0 42-43 36.318731581239646 39.0 35.0 40.0 30.0 41.0 44-45 36.03201566011883 39.0 35.0 40.0 29.5 41.0 46-47 35.807202669150584 39.0 35.0 40.0 28.5 41.0 48-49 35.617865760418624 39.0 35.0 40.0 27.5 41.0 50-51 34.62323203743678 38.0 34.0 39.5 25.5 40.5 52-53 34.73722886472086 38.0 34.5 39.5 26.0 40.5 54-55 35.17499133702318 39.0 35.0 40.0 26.5 41.0 56-57 35.04527716757217 38.5 35.0 40.0 26.0 41.0 58-59 34.81232177316035 38.0 34.0 40.0 26.0 41.0 60-61 34.50846031084986 38.0 34.0 40.0 25.5 41.0 62-63 34.179569203673736 37.5 34.0 40.0 24.0 41.0 64-65 33.83053548323515 37.0 33.5 40.0 23.0 41.0 66-67 33.33435037211062 36.0 33.0 39.0 22.0 41.0 68-69 32.96076863652692 36.0 33.0 39.0 21.5 41.0 70-71 32.52911634457348 35.5 32.0 39.0 20.0 40.0 72-73 32.13313485314268 35.0 32.0 38.0 19.0 40.0 74-75 31.715263052483333 35.0 31.5 37.0 18.0 39.5 76-77 31.24017904545122 35.0 31.0 37.0 13.5 39.0 78-79 30.743689537985166 35.0 31.0 36.0 9.0 39.0 80-81 30.266632915497034 34.0 30.0 36.0 7.0 37.5 82-83 29.840040119914676 34.0 30.0 35.0 3.5 37.0 84-85 29.482666098672098 34.0 29.0 35.0 2.0 36.5 86-87 29.172936065641522 34.0 29.0 35.0 2.0 36.0 88-89 28.75419478822598 34.0 29.0 35.0 2.0 36.0 90-91 28.338878963113203 34.0 29.0 35.0 2.0 35.5 92-93 27.87342755049164 34.0 27.5 35.0 2.0 35.0 94-95 27.354611405881922 34.0 26.5 35.0 2.0 35.0 96-97 26.80949557631844 33.0 25.5 35.0 2.0 35.0 98-99 26.11146548956151 33.0 24.5 35.0 2.0 35.0 100-101 23.275388085466226 30.0 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.39531708146911626 1101 2 0.42125736829503424 1101 3 0.44424749314383405 1101 4 0.49718113825290544 1101 5 0.4384355463773133 1101 6 0.47919783370006996 1101 7 0.5537076761516531 1101 8 0.5470795488493962 1101 9 0.5146679866276571 1101 10-11 0.6633258354387692 1101 12-13 0.6272690194231458 1101 14-15 0.6277398434663581 1101 16-17 0.6680619087991317 1101 18-19 0.7299890741339468 1101 20-21 0.7562493833783321 1101 22-23 0.6713007808340308 1101 24-25 0.6530631047108741 1101 26-27 0.6909155628173593 1101 28-29 0.6142106783825909 1101 30-31 0.5662536162180132 1101 32-33 0.6871143649664404 1101 34-35 0.6397376667723407 1101 36-37 0.6523391382928736 1101 38-39 0.5080944873608289 1101 40-41 0.6652303428798234 1101 42-43 0.9054135507804588 1101 44-45 0.7203441884185935 1101 46-47 0.7182067299444341 1101 48-49 0.9250461534865622 1101 50-51 0.9957759348253035 1101 52-53 1.0633438867449385 1101 54-55 0.7944554510475328 1101 56-57 0.8091745497483416 1101 58-59 0.7591349600583612 1101 60-61 0.9826995538684145 1101 62-63 0.9756960738827587 1101 64-65 0.9289009214214161 1101 66-67 0.832771116387562 1101 68-69 0.7072461298250161 1101 70-71 0.7934716733134692 1101 72-73 0.8544563739839752 1101 74-75 0.6466826960537055 1101 76-77 0.6876610055100052 1101 78-79 0.6261870647574277 1101 80-81 0.8996521989077344 1101 82-83 0.7133353808458871 1101 84-85 0.8747344734029312 1101 86-87 0.7811612124712362 1101 88-89 0.8606256195448623 1101 90-91 0.8613752609874297 1101 92-93 0.623100531251346 1101 94-95 0.6955830993012526 1101 96-97 0.7114532148255073 1101 98-99 0.7752858436030188 1101 100-101 0.9292072658945365 1102 1 0.20601239255803705 1102 2 0.1858087597919038 1102 3 0.2981042551290827 1102 4 0.374137123126431 1102 5 0.36818423746489515 1102 6 0.4141015211468897 1102 7 0.3476293697349462 1102 8 0.40782251355165045 1102 9 0.4603826261130237 1102 10-11 0.47631380219142727 1102 12-13 0.40681511165646356 1102 14-15 0.4706592776208751 1102 16-17 0.41726020349270954 1102 18-19 0.4225340951549299 1102 20-21 0.19835336708409557 1102 22-23 0.1232808197066575 1102 24-25 0.2662847388833356 1102 26-27 0.08889536768177919 1102 28-29 -0.08256352982337489 1102 30-31 0.3041221081487038 1102 32-33 0.06232664789826714 1102 34-35 0.05627483375082676 1102 36-37 -0.16712864070795774 1102 38-39 -0.028192404430818385 1102 40-41 -0.0241811264758951 1102 42-43 0.18505648378936002 1102 44-45 0.31318580916398275 1102 46-47 0.38387886172512964 1102 48-49 0.4829370579510055 1102 50-51 0.5257068342460798 1102 52-53 0.674669884034536 1102 54-55 0.5112299773229054 1102 56-57 0.564818293834243 1102 58-59 0.45717690205385964 1102 60-61 0.42248390280873593 1102 62-63 0.470826488020478 1102 64-65 0.5242524505969044 1102 66-67 0.4836016183351646 1102 68-69 0.37674418221786254 1102 70-71 0.45389083199716396 1102 72-73 0.3272000975089604 1102 74-75 0.4887623701688746 1102 76-77 0.3635880358436019 1102 78-79 0.5418475529258124 1102 80-81 0.3153149590004709 1102 82-83 0.4447354201501774 1102 84-85 0.09159022097395919 1102 86-87 0.3130774897868669 1102 88-89 0.22597803477084355 1102 90-91 0.08155398191674124 1102 92-93 -0.011456176548513497 1102 94-95 0.09669475188766441 1102 96-97 0.19518907635626093 1102 98-99 0.34656349434236233 1102 100-101 0.7836178094410791 1103 1 0.2919127614405497 1103 2 0.21311847150893826 1103 3 0.24929281575753492 1103 4 0.3826560835417965 1103 5 0.3698122827524273 1103 6 0.4351274596810697 1103 7 0.41029780236736 1103 8 0.476608339122393 1103 9 0.21207689511631145 1103 10-11 0.4936939701041183 1103 12-13 0.16954301792892323 1103 14-15 0.40183587054752934 1103 16-17 0.5695243978073208 1103 18-19 0.5962533138975132 1103 20-21 0.680125243714599 1103 22-23 0.3814945672392298 1103 24-25 0.4816216406350762 1103 26-27 0.3668882965213882 1103 28-29 0.430951946093451 1103 30-31 0.22068934802450002 1103 32-33 0.12961249718549794 1103 34-35 0.3251051733343715 1103 36-37 0.2294437803070366 1103 38-39 0.32441294235887597 1103 40-41 0.1791041536157607 1103 42-43 0.21044333610135624 1103 44-45 0.5100829635509854 1103 46-47 0.323894040273764 1103 48-49 0.45685330593054374 1103 50-51 0.4293569832950439 1103 52-53 0.5857969277567818 1103 54-55 0.358714388296697 1103 56-57 0.29619264573575066 1103 58-59 0.29107103897457876 1103 60-61 0.43829330986018533 1103 62-63 0.6061534653365825 1103 64-65 0.534962449100945 1103 66-67 0.6825278089937967 1103 68-69 0.712304675666644 1103 70-71 0.7440911815416271 1103 72-73 0.6548980297965805 1103 74-75 0.49977593964539935 1103 76-77 0.6789475594706929 1103 78-79 0.7724815857129386 1103 80-81 0.7625709620069934 1103 82-83 0.47218998484326846 1103 84-85 0.39598287835106305 1103 86-87 0.4798094679968905 1103 88-89 0.3866360394484616 1103 90-91 0.41678025540061725 1103 92-93 0.3546336322966397 1103 94-95 0.6032810014361374 1103 96-97 0.828927049363255 1103 98-99 0.82810404405393 1103 100-101 0.7301904128905505 1104 1 0.3304235818553849 1104 2 0.35950921687686943 1104 3 0.2783717619883319 1104 4 0.5140115101765304 1104 5 0.46105998076644994 1104 6 0.5480644047187226 1104 7 0.6078959555910117 1104 8 0.6176567478340615 1104 9 0.6612853577787448 1104 10-11 0.699883610518782 1104 12-13 0.6983980338807143 1104 14-15 0.5271438827556167 1104 16-17 0.5046693472717081 1104 18-19 0.5144223596432695 1104 20-21 0.23012092073624046 1104 22-23 0.6019651666634047 1104 24-25 0.5545364520235267 1104 26-27 0.4630157725072408 1104 28-29 0.5258374303967202 1104 30-31 0.6099022201248587 1104 32-33 0.358232343114544 1104 34-35 0.4301029686154081 1104 36-37 0.27522844410901115 1104 38-39 0.20694119302245184 1104 40-41 -0.042583364216525865 1104 42-43 0.12023531438271107 1104 44-45 0.1860212660038485 1104 46-47 0.2036663922333659 1104 48-49 0.34594340544550306 1104 50-51 0.3235819165050273 1104 52-53 0.38372795031408913 1104 54-55 0.2539521953362396 1104 56-57 0.24069424956072538 1104 58-59 0.24315990211772487 1104 60-61 0.14098782227145534 1104 62-63 0.3219051015838943 1104 64-65 0.18223940503250446 1104 66-67 0.24268724043567858 1104 68-69 0.40932095600314256 1104 70-71 0.5245444025928023 1104 72-73 0.2719599607845531 1104 74-75 0.30469505672030195 1104 76-77 0.19315156946541734 1104 78-79 0.4303753441967828 1104 80-81 0.4321817210355299 1104 82-83 0.24528549672713496 1104 84-85 0.1409578483063605 1104 86-87 0.14949804063589767 1104 88-89 0.3836363247650354 1104 90-91 -0.015569888554562539 1104 92-93 -0.033559881506626255 1104 94-95 -0.041587190953688236 1104 96-97 0.3324939388074064 1104 98-99 0.29137126463822227 1104 100-101 0.29299161640772553 1105 1 0.3154034922445845 1105 2 0.2845544077924238 1105 3 0.44789980605696655 1105 4 0.38753477386017465 1105 5 0.31270098927348045 1105 6 0.40350487693166315 1105 7 0.4091972826190329 1105 8 0.5164869242285519 1105 9 0.7582166590201069 1105 10-11 0.526547535746019 1105 12-13 0.49072328853473124 1105 14-15 0.5044177782553803 1105 16-17 0.43083681495270554 1105 18-19 0.3924293760148032 1105 20-21 0.5907979706047399 1105 22-23 0.1785701937779578 1105 24-25 0.18025072549924204 1105 26-27 0.25580471593934817 1105 28-29 0.15541220744059103 1105 30-31 0.16350914054601873 1105 32-33 0.08657175901661418 1105 34-35 0.19348890331774982 1105 36-37 0.1956206841220336 1105 38-39 0.31873516528251145 1105 40-41 0.2555792288424925 1105 42-43 0.26320673487668955 1105 44-45 0.645987683370862 1105 46-47 0.5764175552033706 1105 48-49 0.7930068526342424 1105 50-51 0.8017103700761865 1105 52-53 0.826709222855996 1105 54-55 0.8336670428008333 1105 56-57 0.6440531553837161 1105 58-59 0.7895801819158947 1105 60-61 0.8113479360411944 1105 62-63 0.5700846144643705 1105 64-65 0.5725989619473495 1105 66-67 0.5060774586371295 1105 68-69 0.7644883551481598 1105 70-71 0.44454148981341035 1105 72-73 0.4313457498479245 1105 74-75 0.7189329793945767 1105 76-77 0.32627201289324503 1105 78-79 0.6268905035001175 1105 80-81 0.8046437478159696 1105 82-83 0.5056579898092828 1105 84-85 0.5541752273189324 1105 86-87 0.3522617542582829 1105 88-89 0.17467867143718507 1105 90-91 0.5152531001284331 1105 92-93 0.34212547835013396 1105 94-95 0.6874996955421508 1105 96-97 0.9725632886579128 1105 98-99 1.0133454008706302 1105 100-101 0.9212941942174844 1106 1 0.24385577017928384 1106 2 0.37006743972323974 1106 3 0.3862695765491324 1106 4 0.3940180846073673 1106 5 0.46004698173025105 1106 6 0.5712627535079307 1106 7 0.44432009135524453 1106 8 0.5634266382825714 1106 9 0.5923309829869936 1106 10-11 0.711957977618006 1106 12-13 0.6957850330555573 1106 14-15 0.6268074463207469 1106 16-17 0.5914283541301373 1106 18-19 0.526086942916038 1106 20-21 0.5031392553479321 1106 22-23 0.6045563645291807 1106 24-25 0.49613694577669776 1106 26-27 0.5076313360186973 1106 28-29 0.4129411476335747 1106 30-31 0.5527969151853185 1106 32-33 0.7779001778235184 1106 34-35 0.5905967888740449 1106 36-37 0.4224923404208454 1106 38-39 0.5016087482063014 1106 40-41 0.5238787646020668 1106 42-43 0.5763996259570661 1106 44-45 0.3293136694055505 1106 46-47 0.40315396678271753 1106 48-49 0.5655623791634312 1106 50-51 0.34397774643747425 1106 52-53 0.37622661418401293 1106 54-55 0.5004609265829032 1106 56-57 0.27569973943408854 1106 58-59 0.16795925898633612 1106 60-61 0.5172593820439886 1106 62-63 0.4123353913731762 1106 64-65 0.42219159505591364 1106 66-67 0.21577344374659901 1106 68-69 0.36284487329054116 1106 70-71 0.31378134373823485 1106 72-73 0.3345199189228758 1106 74-75 0.5242894322276364 1106 76-77 0.2893423129351511 1106 78-79 0.3485387747079578 1106 80-81 0.4725356445619333 1106 82-83 0.29039302445043447 1106 84-85 0.5034890712623081 1106 86-87 0.5883944083746719 1106 88-89 0.6572506069333457 1106 90-91 0.49009608563520857 1106 92-93 0.7198984032825066 1106 94-95 0.8341225233269114 1106 96-97 0.8945356573725718 1106 98-99 1.1692584796064622 1106 100-101 1.00195732873836 1107 1 0.17789778448202753 1107 2 0.2416034945504144 1107 3 0.36550825354555627 1107 4 0.28062267340354907 1107 5 0.3273756468077238 1107 6 0.3299546486211611 1107 7 0.35729088992496827 1107 8 0.5333134082468192 1107 9 0.46188104258174434 1107 10-11 0.5574094078921377 1107 12-13 0.4431576492653235 1107 14-15 0.39267976455675324 1107 16-17 0.5594840752266776 1107 18-19 0.6162821157408302 1107 20-21 0.46066383818464374 1107 22-23 0.5519006732300156 1107 24-25 0.5077564332617968 1107 26-27 0.4644862227886648 1107 28-29 0.332475563604973 1107 30-31 0.39633162913049347 1107 32-33 0.4922766676924084 1107 34-35 0.539373606514097 1107 36-37 0.740814748048976 1107 38-39 0.6595713524971245 1107 40-41 0.9712498909429428 1107 42-43 0.7386605019999735 1107 44-45 0.9910247838274842 1107 46-47 0.955218925066383 1107 48-49 0.9687916699858405 1107 50-51 0.8370528358296099 1107 52-53 1.0650682411208408 1107 54-55 0.9341550564994705 1107 56-57 0.8362934521873697 1107 58-59 0.9640721910483165 1107 60-61 0.764024876680466 1107 62-63 0.8247392948296692 1107 64-65 0.6936606057829664 1107 66-67 0.6762144449384948 1107 68-69 0.718292578892445 1107 70-71 0.6471499373019967 1107 72-73 0.7534233754187021 1107 74-75 0.6737217921799576 1107 76-77 0.865975209240279 1107 78-79 1.0240080834087912 1107 80-81 0.9989160080899389 1107 82-83 1.0363115286220683 1107 84-85 0.8867950903326296 1107 86-87 0.9202098136190138 1107 88-89 0.8679292422134424 1107 90-91 0.7533067530964708 1107 92-93 0.9443657751537842 1107 94-95 1.0146030060444353 1107 96-97 0.9102202521282372 1107 98-99 0.982657615482502 1107 100-101 0.7829294681900905 1108 1 0.22199449981049213 1108 2 0.2161929106088074 1108 3 0.14858307106380053 1108 4 0.07362449822106498 1108 5 -0.0017575648711058989 1108 6 0.24398749533649067 1108 7 0.14927994101985576 1108 8 0.04787545204244026 1108 9 0.4078116995160528 1108 10-11 0.32875064876805027 1108 12-13 0.2407899485354008 1108 14-15 0.27835038499470244 1108 16-17 0.19217568106609662 1108 18-19 0.47579853909849135 1108 20-21 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-1.2541580628939073 2316 64-65 -0.8558312641357304 2316 66-67 -1.100574172947681 2316 68-69 -1.6334401446847906 2316 70-71 -1.420421200909388 2316 72-73 -1.29511320994715 2316 74-75 -1.2200078826167875 2316 76-77 -1.1934963354751709 2316 78-79 -2.3629329735017777 2316 80-81 -2.085641918739327 2316 82-83 -1.6435512762559803 2316 84-85 -1.515389869016964 2316 86-87 -1.3539416091452132 2316 88-89 -1.8848908797377817 2316 90-91 -1.7062359298303562 2316 92-93 -2.8210051598055657 2316 94-95 -2.6901175224108513 2316 96-97 -2.571664910473391 2316 98-99 -4.113882018663837 2316 100-101 -5.100144718801783 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1307.0 3 447.0 4 443.0 5 482.0 6 518.0 7 598.0 8 751.0 9 856.0 10 1074.0 11 1491.0 12 1974.0 13 2690.0 14 3029.0 15 3857.0 16 5069.0 17 6938.0 18 5474.0 19 3644.0 20 3475.0 21 3655.0 22 4038.0 23 4453.0 24 5061.0 25 5726.0 26 6735.0 27 7929.0 28 9845.0 29 11866.0 30 14762.0 31 18504.0 32 23815.0 33 31762.0 34 44463.0 35 63160.0 36 90539.0 37 123248.0 38 99939.0 39 15493.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.52640280815994 17.835885435213918 14.285464465584779 22.352247291041362 2 15.195179918446508 19.871231330530097 40.49424912763201 24.439339623391383 3 20.475363811813033 24.200122727865267 29.808722580973367 25.515790879348334 4 12.213207670159607 15.699947375366266 38.29594758522885 33.79089736924528 5 13.139257172114855 37.38817670386425 34.529984385285914 14.942581738734987 6 31.2901358456239 35.60612084567161 17.64863979440269 15.455103514301799 7 28.189916639642902 32.83895745506108 20.736604162295176 18.234521743000844 8 25.94243247669458 33.06086041235048 19.816800253127926 21.17990685782701 9 25.651658861972187 17.479388451172294 21.432353572535956 35.43659911431956 10-11 25.079134116524536 25.754904884012163 27.128073627924543 22.03788737153875 12-13 26.979914435798623 23.352539297756582 27.593938155575465 22.073608110869326 14-15 23.00916827262493 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21.87242693693035 40-41 21.6156564905942 26.325590341222032 30.329284469322758 21.729468698861005 42-43 21.28966418373089 26.446683643461867 29.3865463666797 22.877105806127542 44-45 21.65641797189497 26.19604533110159 29.184499554525523 22.96303714247792 46-47 22.36550234895515 26.61770327741037 28.920958910968597 22.09583546266588 48-49 22.27245199162625 26.488891432168355 28.858274162347065 22.380382413858328 50-51 21.413623592714902 26.975285828395666 29.468957267810374 22.142133311079053 52-53 22.05072488533213 27.400117151579362 28.27843608205061 22.270721881037897 54-55 21.28945291481246 26.62431438501896 28.912704651253364 23.173528048915216 56-57 22.138753269502367 26.675483361799497 28.382340435170665 22.80342293352747 58-59 22.826229502984887 26.40491634285006 28.374362467422948 22.394491686742104 60-61 22.063813130610672 26.352462436566377 29.658044595276607 21.925679837546348 62-63 21.71128919323394 26.73878436300141 29.297555834785783 22.252370608978865 64-65 21.37999933238914 27.529331165187592 28.766477669211536 22.32419183321173 66-67 21.498799329436817 27.059481879452836 28.114132460869907 23.327586330240443 68-69 21.162009485304935 26.82806791573742 28.858145793827795 23.151776805129856 70-71 22.017183592492017 26.775074646448182 28.208127417809393 22.99961434325041 72-73 21.843184636418556 26.21856220787892 29.227767002639027 22.710486153063496 74-75 21.30710411707745 27.619781306595304 28.457173204095294 22.615941372231948 76-77 21.604670816065152 26.7613942169713 28.256958484019997 23.376976482943558 78-79 21.681373001205557 26.691997188089463 28.687769779595833 22.938860031109147 80-81 22.0751960077027 26.9919131163097 28.15614756673648 22.776743309251117 82-83 21.776198764796952 26.681864843309555 28.882552339482842 22.65938405241065 84-85 21.563893828383286 27.047037323499744 28.34330020405389 23.04576864406308 86-87 21.510923584194746 27.259119944801796 28.43335357691342 22.79660289409004 88-89 21.475450495568975 27.051436148394053 27.800831194930154 23.67228216110682 90-91 21.484956682336872 26.650956709368174 28.166617771809687 23.697468836485264 92-93 22.036668426106697 26.977223730818395 28.312225352470954 22.673882490603955 94-95 21.630264230970393 26.743509482014456 28.274510240321256 23.351716046693895 96-97 20.95702566059334 26.76054882508188 28.857594836083816 23.424830678240962 98-99 21.474335271239234 27.404470463435445 27.55444545435027 23.566748810975053 100-101 21.421191569439817 26.76692518708994 27.878210394954834 23.93367284851541 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 73.0 1 74.5 2 205.5 3 279.0 4 176.5 5 149.0 6 273.5 7 566.5 8 956.5 9 1107.0 10 981.5 11 858.5 12 837.0 13 903.5 14 972.0 15 999.5 16 1024.0 17 1090.0 18 1133.0 19 1212.5 20 1359.0 21 1522.5 22 1765.5 23 2078.5 24 2524.5 25 3065.0 26 3739.0 27 4546.5 28 5303.0 29 6087.5 30 7162.0 31 8488.5 32 9952.0 33 10948.0 34 11918.0 35 12979.0 36 14132.0 37 15382.5 38 16073.5 39 16343.0 40 16575.0 41 17660.5 42 18829.5 43 19714.5 44 20532.5 45 21475.5 46 22435.5 47 26143.5 48 35624.0 49 35308.0 50 26253.5 51 22520.0 52 20760.5 53 20045.5 54 19366.5 55 17700.5 56 15784.5 57 13968.5 58 12536.0 59 11076.0 60 9386.5 61 7901.5 62 6561.5 63 5080.0 64 3810.0 65 3016.0 66 2325.5 67 1829.0 68 1392.5 69 958.5 70 689.0 71 544.0 72 455.5 73 416.0 74 333.0 75 269.0 76 201.5 77 133.0 78 97.5 79 59.0 80 45.0 81 31.5 82 19.5 83 10.5 84 6.0 85 5.5 86 5.0 87 5.0 88 2.0 89 1.0 90 2.0 91 2.5 92 1.5 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014305833282997994 2 0.012557342548409352 3 0.012716296251553773 4 0.020822935111919304 5 0.03496981469177288 6 0.04959355538105971 7 0.006040240719488042 8 0.029088527675429257 9 0.03481086098862845 10-11 0.04395069891943272 12-13 0.036479874871644884 14-15 0.03274446284775096 16-17 0.03020120359744021 18-19 0.023763578620091114 20-21 0.051262569264076144 22-23 0.01168309718111503 24-25 0.00294064350817181 26-27 9.537222188665329E-4 28-29 7.947685157221107E-5 30-31 0.0023843055471663324 32-33 0.001986921289305277 34-35 0.0 36-37 3.973842578610554E-4 38-39 0.0 40-41 0.0011921527735831662 42-43 0.003179074062888443 44-45 0.0015100601798720104 46-47 7.152916641498996E-4 48-49 7.947685157221108E-4 50-51 0.0034175046176050765 52-53 0.0023048286955941215 54-55 0.004053319430182765 56-57 7.152916641498996E-4 58-59 0.0045301805396160315 60-61 0.0018279675861608547 62-63 5.563379610054775E-4 64-65 7.947685157221108E-4 66-67 0.014385310134570206 68-69 0.03918208782510006 70-71 0.05030884704520962 72-73 0.014941648095575683 74-75 0.031313879519451165 76-77 0.029803819339579155 78-79 0.057223333131991974 80-81 0.08003318953321656 82-83 0.049355124826343084 84-85 0.018041245306891916 86-87 0.017166999939597593 88-89 0.0222535184402191 90-91 0.03393661562133413 92-93 0.020028166596197192 94-95 0.012875249954698196 96-97 0.021299796221352568 98-99 0.05444164332696459 100-101 0.07510562473573947 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 629114.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.51811812138546 #Duplication Level Percentage of deduplicated Percentage of total 1 86.85458889885827 57.77403803758627 2 7.984084140497667 10.621725038974084 3 2.0950327279177836 4.180729033914106 4 0.902278293507853 2.4007141642366987 5 0.5127798475059145 1.7054575233332223 6 0.3227650208980191 1.2881833071327529 7 0.22683943491656933 1.056225262845808 8 0.16419700356193162 0.8737660542488094 9 0.1304865266160674 0.7811746371627192 >10 0.7292642695424946 8.891489520456027 >50 0.04756644471433162 2.1734813254118217 >100 0.02438074547022383 2.8611367373281924 >500 0.003346376829223012 1.5201627310630537 >1k 0.0023902691637307234 3.8717166263064975 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4175 0.6636317106279624 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3948 0.6275492200141787 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3312 0.5264546648143261 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2556 0.40628566523714305 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2465 0.3918208782510006 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2429 0.38609854493780144 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1582 0.25146475837447585 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1540 0.24478870284241014 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1198 0.19042653636701773 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1146 0.18216094380350778 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 916 0.1456015920802907 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 822 0.130659943984715 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 813 0.12922936065641522 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 784 0.12461970326522696 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 701 0.11142654590423992 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 684 0.10872433295078475 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 682 0.10840642554449591 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 651 0.10347886074701883 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 7.947685157221107E-5 0.0 22-23 0.0 0.0 0.0 3.179074062888443E-4 0.0 24-25 0.0 0.0 0.0 3.179074062888443E-4 0.0 26-27 0.0 0.0 0.0 4.7686110943326646E-4 0.0 28-29 0.0 0.0 0.0 5.563379610054775E-4 0.0 30-31 0.0 0.0 0.0 0.002145874992449699 0.0 32-33 0.0 0.0 0.0 0.006437624977349097 0.0 34-35 0.0 0.0 0.0 0.012318911993692717 0.0 36-37 0.0 0.0 0.0 0.02137927307292478 0.0 38-39 0.0 0.0 0.0 0.03814888875466132 0.0 40-41 0.0 0.0 0.0 0.05793862479614187 0.0 42-43 0.0 0.0 0.0 0.08901407376087642 0.0 44-45 0.0 0.0 0.0 0.12811668473440427 0.0 46-47 0.0 0.0 0.0 0.1763591336387364 0.0 48-49 0.0 0.0 0.0 0.2333440362160117 0.0 50-51 0.0 0.0 0.0 0.3012967443102522 0.0 52-53 0.0 0.0 0.0 0.36885206814663163 0.0 54-55 0.0 0.0 0.0 0.4426860632572157 0.0 56-57 0.0 0.0 0.0 0.5457675397463735 0.0 58-59 0.0 0.0 0.0 0.6664928772845622 0.0 60-61 0.0 0.0 0.0 0.7995371268164435 0.0 62-63 0.0 0.0 0.0 0.9518147744287999 0.0 64-65 0.0 0.0 0.0 1.0966215979933684 0.0 66-67 0.0 0.0 0.0 1.280610509383037 0.0 68-69 0.0 0.0 0.0 1.4950390549248627 0.0 70-71 0.0 0.0 0.0 1.7314032115006182 0.0 72-73 0.0 0.0 0.0 1.9987633401895364 0.0 74-75 0.0 0.0 0.0 2.2755017373639754 0.0 76-77 0.0 0.0 0.0 2.5624131715396574 0.0 78-79 0.0 0.0 0.0 2.903766249042304 0.0 80-81 0.0 0.0 0.0 3.3235629790467227 0.0 82-83 0.0 0.0 0.0 3.713237982305274 0.0 84-85 0.0 0.0 0.0 4.1632359159071335 7.947685157221107E-5 86-87 0.0 0.0 0.0 4.651462215115226 1.5895370314442214E-4 88-89 0.0 0.0 0.0 5.195640217830155 1.5895370314442214E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCGT 60 8.277883E-5 39.590294 6 TACCTGG 485 0.0 34.284378 2 GTACCTG 595 0.0 29.54536 1 ATTCGCG 100 7.1304385E-10 26.141031 80-81 GTACATG 2910 0.0 26.123508 1 GTAGTAC 110 9.463736E-5 25.911585 3 GTAAGCG 65 9.99604E-6 25.585491 94-95 TACATGG 2995 0.0 25.221464 2 ATGGGAG 740 0.0 25.036194 5 GAGTACT 2875 0.0 23.958828 12-13 ACATGGG 2985 0.0 23.871643 3 GTACTTT 3095 0.0 22.17815 14-15 CATGGGG 1310 0.0 21.75782 4 GTGTAAG 175 4.0354716E-6 21.719833 1 TCCGCGT 55 0.0028653047 21.606737 64-65 CATGGGA 1580 0.0 21.346992 4 ACAGTCC 245 7.494236E-9 21.328585 8 GTAGCCC 275 1.2532837E-9 20.729269 3 AGTACTT 3030 0.0 20.695063 12-13 CCCGCGA 140 1.5734258E-9 20.360723 20-21 >>END_MODULE