FastQCFastQC Report
Thu 26 May 2016
SRR936399_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936399_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629114
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT80221.2751266066245543No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29460.46827760946346764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25720.4088289244874537No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19050.3028068044901242No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT16690.2652937305480406No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16650.2646579157354629No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC14810.23541043435688921No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT13230.2102957492600705No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC13190.20965993444749284No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA12450.1978973604148056No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT12100.1923339808047508No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA12010.19090339747645102No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA11910.18931386044500678No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG11840.18820118452299583No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC11320.17993559195948589No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA9320.14814485133060146No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7240.11508248107656165No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGCG853.6379788E-1233.529294-95
TACCGTG753.077706E-431.6652097
TACCTGG5900.028.993242
GTACACG1405.785314E-727.150241
GATCGAT350.00839049727.14376630-31
GTACCTG6900.026.855131
TAAGGTG3050.024.9188675
GTAGGGG1503.027754E-522.1691723
GTACATG27600.020.6577911
GTGTAGG2308.97835E-820.6577911
AGTACTT31150.020.58656312-13
GAGTACT30550.020.29118712-13
GTAAGGT2351.1294287E-720.2134424
CTGGACG2351.1294287E-720.2134424
GGTGTGC5700.019.9990798
GTACTAT1707.929961E-519.5641441
CTACACT3655.456968E-1219.52124
TACACTA2201.3423396E-619.4324685
TACATGG28700.019.2053572
GTACTTT32200.019.1776614-15
Produced by FastQC (version 0.11.5)