##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936399_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 629114 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.69362150580022 31.0 31.0 34.0 30.0 34.0 2 31.827813401068806 31.0 31.0 34.0 30.0 34.0 3 31.925962544149392 31.0 31.0 34.0 30.0 34.0 4 35.69760329606399 37.0 35.0 37.0 33.0 37.0 5 35.54539240900695 37.0 35.0 37.0 33.0 37.0 6 35.62254217836513 37.0 35.0 37.0 33.0 37.0 7 35.54122464291051 37.0 35.0 37.0 33.0 37.0 8 35.5138861955067 37.0 35.0 37.0 33.0 37.0 9 37.07252262705964 39.0 37.0 39.0 33.0 39.0 10-11 36.9498636177227 39.0 37.0 39.0 33.0 39.0 12-13 36.813347024545635 39.0 37.0 39.0 32.5 39.0 14-15 37.78252590150593 39.0 37.0 41.0 33.0 41.0 16-17 37.77587845764043 39.0 37.0 41.0 32.5 41.0 18-19 37.96372279109986 40.0 37.0 41.0 33.0 41.0 20-21 37.967226130717165 40.0 37.0 41.0 33.0 41.0 22-23 37.8507583681177 40.0 37.0 41.0 32.5 41.0 24-25 37.696764815279906 39.0 37.0 41.0 32.0 41.0 26-27 37.431767533388225 39.0 36.0 41.0 32.0 41.0 28-29 37.451800468595515 39.0 36.0 41.0 32.0 41.0 30-31 37.32846829032576 39.0 36.0 41.0 32.0 41.0 32-33 37.10827846781346 39.0 36.0 41.0 31.0 41.0 34-35 36.8606111134071 39.0 35.0 40.0 31.0 41.0 36-37 36.6286118255197 39.0 35.0 40.0 30.0 41.0 38-39 36.398965847207336 38.0 35.0 40.0 30.0 41.0 40-41 36.17751711136614 38.0 35.0 40.0 30.0 41.0 42-43 35.889834751730206 38.0 35.0 40.0 29.5 41.0 44-45 35.55391073795846 38.0 35.0 40.0 28.0 41.0 46-47 35.109397501883606 38.0 34.0 40.0 26.5 41.0 48-49 34.62377804340708 38.0 33.5 40.0 24.5 41.0 50-51 34.27298152640062 38.0 33.0 40.0 24.0 41.0 52-53 34.00830135714672 38.0 33.0 40.0 23.5 41.0 54-55 33.66116315961813 37.0 33.0 40.0 23.0 41.0 56-57 33.28120738053835 37.0 32.0 39.5 21.5 40.5 58-59 32.862549553816955 36.0 31.5 39.0 20.0 40.0 60-61 32.37710653395092 36.0 31.0 39.0 18.5 40.0 62-63 32.04350880762469 35.0 31.0 39.0 18.0 40.0 64-65 31.689223256834214 35.0 30.5 38.5 15.5 40.0 66-67 31.173375572630718 35.0 29.5 38.0 12.0 40.0 68-69 30.649898110676283 34.0 29.0 37.5 8.0 39.5 70-71 30.06020530460298 34.0 28.5 37.0 7.5 39.0 72-73 29.429216326452757 34.0 28.0 36.0 6.5 39.0 74-75 28.860670721045786 33.0 27.0 35.5 2.0 38.0 76-77 27.63729308201693 31.5 26.0 34.0 2.0 36.0 78-79 28.422621337309295 33.0 27.0 35.0 2.0 37.0 80-81 28.196187813337488 33.0 26.5 35.0 2.0 36.5 82-83 27.713551915868983 33.0 26.0 35.0 2.0 36.0 84-85 27.21044278143548 32.0 26.0 35.0 2.0 36.0 86-87 26.726354047120235 32.0 25.0 34.0 2.0 35.0 88-89 26.114805106864576 32.0 24.5 34.0 2.0 35.0 90-91 25.3983975877186 31.0 21.5 34.0 2.0 35.0 92-93 24.675368852068146 31.0 19.0 34.0 2.0 35.0 94-95 23.75104591536669 30.5 11.0 34.0 2.0 35.0 96-97 22.705815639136944 30.0 2.0 34.0 2.0 35.0 98-99 21.28793191694987 29.0 2.0 34.0 2.0 35.0 100-101 17.55188010440079 22.5 2.0 31.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21704761135581307 1101 2 0.4110588670405342 1101 3 0.30014635544625534 1101 4 0.27287931201723126 1101 5 0.28907810572674464 1101 6 0.22672316281978766 1101 7 0.21783589309018225 1101 8 0.2670500295266365 1101 9 0.3898739504567317 1101 10-11 0.28720534988920576 1101 12-13 0.3429654628738845 1101 14-15 0.4412097908475445 1101 16-17 0.3720175580989178 1101 18-19 0.284057209710852 1101 20-21 0.30854066171001193 1101 22-23 0.3234940853196022 1101 24-25 0.43824348372024247 1101 26-27 0.37030234344969415 1101 28-29 0.4007609686557245 1101 30-31 0.5687742405190619 1101 32-33 0.5408949318858447 1101 34-35 0.5169806097938192 1101 36-37 0.4915018462040379 1101 38-39 0.45012561456781697 1101 40-41 0.44745920875355694 1101 42-43 0.5811078847465723 1101 44-45 0.568541161541674 1101 46-47 0.48465073030669004 1101 48-49 0.5660518734067921 1101 50-51 0.7277605228875643 1101 52-53 0.8260975914202362 1101 54-55 0.8185354369618523 1101 56-57 0.7541692163697391 1101 58-59 0.7401890937250002 1101 60-61 0.7636861299952287 1101 62-63 0.7944514037562094 1101 64-65 0.8104238168020039 1101 66-67 0.8420377971883148 1101 68-69 0.7499548296072653 1101 70-71 0.6046449757014436 1101 72-73 0.9468363331932892 1101 74-75 1.042664223879509 1101 76-77 0.9385053241513361 1101 78-79 1.0288207132192824 1101 80-81 1.0317803403706236 1101 82-83 0.9780077710084889 1101 84-85 1.0805433200254697 1101 86-87 0.9260570768988394 1101 88-89 0.8381544617921008 1101 90-91 0.9606148258278999 1101 92-93 0.9282215300644943 1101 94-95 0.7402535177697338 1101 96-97 0.8887729784636136 1101 98-99 0.5068106027431831 1101 100-101 0.41204464884583913 1102 1 0.1751340149109204 1102 2 0.392972077258289 1102 3 0.300918809726614 1102 4 0.20594955651036884 1102 5 0.010335138752573414 1102 6 0.116414419659705 1102 7 0.17356631109018394 1102 8 0.11557344909085288 1102 9 0.0830039919988721 1102 10-11 0.32316082471572116 1102 12-13 0.3081152792455981 1102 14-15 0.47304910672568923 1102 16-17 0.4943720441839474 1102 18-19 0.36067700657809354 1102 20-21 0.3821745819950024 1102 22-23 0.12194003226335326 1102 24-25 0.19643632693133384 1102 26-27 0.2725246830546695 1102 28-29 0.19859037212790298 1102 30-31 0.44963878725312156 1102 32-33 0.36547113279666377 1102 34-35 0.5524703401277549 1102 36-37 0.5316424896811682 1102 38-39 0.5171096122686336 1102 40-41 0.7073224843459229 1102 42-43 0.7174741802944027 1102 44-45 0.7529120827274767 1102 46-47 0.6465489117448371 1102 48-49 0.48372926242045367 1102 50-51 0.4869220578450921 1102 52-53 0.8216358159795121 1102 54-55 0.9046714729763963 1102 56-57 0.8098688497947464 1102 58-59 0.6302168793598426 1102 60-61 0.6337107576552299 1102 62-63 0.5972165628874642 1102 64-65 0.5120287041656688 1102 66-67 0.7138350938255265 1102 68-69 0.6706672255673283 1102 70-71 0.4106777300348554 1102 72-73 -0.08047702538112489 1102 74-75 0.2815539458073282 1102 76-77 0.33422626822678225 1102 78-79 0.4045828018447786 1102 80-81 0.6905895566589564 1102 82-83 0.7401634182740544 1102 84-85 0.29659225046028226 1102 86-87 0.3143343448189242 1102 88-89 0.41412931613335147 1102 90-91 0.33313658234349575 1102 92-93 0.46526696059984474 1102 94-95 0.5247458733122627 1102 96-97 0.580166985808745 1102 98-99 0.6355744714214104 1102 100-101 0.7247878157493517 1103 1 0.3035182657072468 1103 2 0.30509849705461534 1103 3 0.3440937534455806 1103 4 0.166962578683453 1103 5 0.3548101105664472 1103 6 0.18602338959814801 1103 7 0.17648818170035696 1103 8 0.23286203489526258 1103 9 0.13319390629115446 1103 10-11 0.27831405143434296 1103 12-13 0.18655517255791665 1103 14-15 0.5382325810808695 1103 16-17 0.2968454908455769 1103 18-19 0.20896084543736748 1103 20-21 0.2560018019489618 1103 22-23 0.2715899404988136 1103 24-25 0.1960944447183266 1103 26-27 0.2007987434258922 1103 28-29 0.23215357290434468 1103 30-31 0.2608617915111324 1103 32-33 0.2268088883417576 1103 34-35 0.20564962578731638 1103 36-37 0.4643767081865917 1103 38-39 0.2577848387610331 1103 40-41 0.4964869709205715 1103 42-43 0.6009219000458472 1103 44-45 0.43507217992856795 1103 46-47 0.4313845930099163 1103 48-49 0.40738371692311404 1103 50-51 0.27907226709743327 1103 52-53 0.45074469652506366 1103 54-55 0.5330677644483188 1103 56-57 0.31963675665100055 1103 58-59 0.30826855210158044 1103 60-61 0.24473162451089792 1103 62-63 0.340627576821813 1103 64-65 0.44881150862144636 1103 66-67 0.26695928427252724 1103 68-69 0.2524219533568406 1103 70-71 0.05418579911184551 1103 72-73 0.5127736286464817 1103 74-75 0.6047198407761485 1103 76-77 0.47522622809119497 1103 78-79 0.12940848570042363 1103 80-81 0.2925992548758387 1103 82-83 0.41287742303924446 1103 84-85 0.5186146461168804 1103 86-87 0.12755427688032484 1103 88-89 0.1671232483625893 1103 90-91 0.15548553571688828 1103 92-93 0.5312159300274715 1103 94-95 0.28871433403959657 1103 96-97 0.2652690214126565 1103 98-99 0.38439028922044827 1103 100-101 -0.01843456093406104 1104 1 0.301530560257703 1104 2 0.30124758793226647 1104 3 0.28888931979897237 1104 4 0.38180768636884466 1104 5 0.13076224655004864 1104 6 0.20382282069419944 1104 7 0.15222503336167392 1104 8 0.29712397280000147 1104 9 0.36101400122375793 1104 10-11 0.3121474092645471 1104 12-13 0.2723606731155783 1104 14-15 0.42838008110248893 1104 16-17 0.2417063341721999 1104 18-19 0.32367756066549447 1104 20-21 0.39139575496682966 1104 22-23 0.29546185929599034 1104 24-25 0.3242659977915352 1104 26-27 0.44943268226439415 1104 28-29 0.4143632200628531 1104 30-31 0.36493360482764103 1104 32-33 0.4324080099125496 1104 34-35 0.5298103195388819 1104 36-37 0.5869383322431005 1104 38-39 0.5702834337930724 1104 40-41 0.43807834571415327 1104 42-43 0.6744198812149591 1104 44-45 0.8404482358160337 1104 46-47 0.7514259206830332 1104 48-49 0.7044747847852264 1104 50-51 0.8149473676066918 1104 52-53 0.9086215953933063 1104 54-55 0.8233086408024874 1104 56-57 1.0041968071433942 1104 58-59 1.180813748840329 1104 60-61 0.6748714296310325 1104 62-63 0.7157073357725459 1104 64-65 0.6570191153341689 1104 66-67 0.9676034080480456 1104 68-69 1.0492043605641115 1104 70-71 0.9067363563437603 1104 72-73 1.0571442462271712 1104 74-75 1.1389284331279335 1104 76-77 1.2029628895414675 1104 78-79 1.3923843375433087 1104 80-81 1.2136863110140368 1104 82-83 1.0450533509665512 1104 84-85 0.9322705024556619 1104 86-87 1.1225309760269688 1104 88-89 1.0529486346207086 1104 90-91 1.100886319040569 1104 92-93 1.2813006604033141 1104 94-95 1.2673752482630576 1104 96-97 1.6270193088907163 1104 98-99 1.6956970391188584 1104 100-101 1.2092800533265802 1105 1 0.19243626330840868 1105 2 0.3060622409260034 1105 3 0.246688286618987 1105 4 0.1695581639789978 1105 5 0.19934749246573347 1105 6 0.17656691589068174 1105 7 0.2101529335047445 1105 8 0.30082491602650663 1105 9 0.43047522270636307 1105 10-11 0.45209253764101476 1105 12-13 0.25579638207752 1105 14-15 0.4947047103656388 1105 16-17 0.45031381144823257 1105 18-19 0.35584235544305187 1105 20-21 0.29610396300780906 1105 22-23 0.2697887074116778 1105 24-25 0.2753634302621677 1105 26-27 0.43822062335771506 1105 28-29 0.4171881877224308 1105 30-31 0.4290650825491582 1105 32-33 0.541712198317974 1105 34-35 0.38499699442986923 1105 36-37 0.5695794521211113 1105 38-39 0.6000890914386829 1105 40-41 0.4846153508629172 1105 42-43 0.649822958480712 1105 44-45 0.40760600565506167 1105 46-47 0.6246546938032154 1105 48-49 0.674152965497484 1105 50-51 0.6011857700328562 1105 52-53 0.8857498519234994 1105 54-55 0.7583697234997189 1105 56-57 0.7033709485159889 1105 58-59 0.8641646445199882 1105 60-61 0.5875619382537209 1105 62-63 0.7860445328220713 1105 64-65 0.687901002129852 1105 66-67 0.8728416991032404 1105 68-69 0.5122056370468435 1105 70-71 0.618692459044933 1105 72-73 0.9040862562162921 1105 74-75 0.9062380530102239 1105 76-77 0.5259569269107125 1105 78-79 0.5776446029489151 1105 80-81 1.1021504195750396 1105 82-83 0.8636707231255194 1105 84-85 0.7515239742316844 1105 86-87 0.657915891256522 1105 88-89 0.9110598685254594 1105 90-91 0.9685786695678651 1105 92-93 1.0192222638026962 1105 94-95 1.0801274392728502 1105 96-97 1.093903577311906 1105 98-99 1.0594849802121793 1105 100-101 0.6558594651164817 1106 1 0.13494365142537745 1106 2 0.3404258288024202 1106 3 0.22587094046325618 1106 4 0.2689086083545007 1106 5 0.196701104343326 1106 6 -0.014299575529307162 1106 7 0.2386128308602622 1106 8 0.15286664868279587 1106 9 0.0676792218138047 1106 10-11 0.1546596021502964 1106 12-13 0.2225498557170411 1106 14-15 0.21401953394883577 1106 16-17 0.42074161471479243 1106 18-19 0.41897605995102793 1106 20-21 0.23358593491391844 1106 22-23 0.18734657419154388 1106 24-25 0.26454657392635283 1106 26-27 0.28609888678286666 1106 28-29 0.4771149324337358 1106 30-31 0.5317592281127475 1106 32-33 0.4234710327642901 1106 34-35 0.4509355982254277 1106 36-37 0.33565643004111934 1106 38-39 0.6083514008851338 1106 40-41 0.3737942766334328 1106 42-43 0.4688923278647934 1106 44-45 0.4808783019852214 1106 46-47 0.30541116622907793 1106 48-49 0.06681369445836083 1106 50-51 0.14537568953912228 1106 52-53 0.2505255416171437 1106 54-55 0.12808102908747543 1106 56-57 0.1107596097293353 1106 58-59 0.28535670290651893 1106 60-61 0.13859913191217998 1106 62-63 0.07084623739535445 1106 64-65 0.055362537660844424 1106 66-67 -0.08191390797411202 1106 68-69 -0.029333812504425794 1106 70-71 -0.1106126497767228 1106 72-73 0.4265540233450231 1106 74-75 0.462114259191754 1106 76-77 0.4222967476366257 1106 78-79 0.1223032528963941 1106 80-81 0.28928611601731546 1106 82-83 0.5074315059665828 1106 84-85 0.585922665868285 1106 86-87 0.7202529581060553 1106 88-89 0.6096491871859655 1106 90-91 0.5220948962129803 1106 92-93 0.5029064986767828 1106 94-95 0.5056156264493623 1106 96-97 0.287880425157514 1106 98-99 0.44416300525830366 1106 100-101 0.6286057492882229 1107 1 0.14503900303443729 1107 2 0.3231284509716694 1107 3 0.12944513593405915 1107 4 0.07367916854520473 1107 5 0.11165342853879423 1107 6 0.12803951863040908 1107 7 0.045278504281000664 1107 8 0.14104751876622856 1107 9 -0.048251946243411226 1107 10-11 0.2483082910656762 1107 12-13 0.3178426149542304 1107 14-15 0.4225557377628988 1107 16-17 0.3260015968363632 1107 18-19 0.2068611910594882 1107 20-21 0.1790634378936531 1107 22-23 0.3399799174573346 1107 24-25 0.28792336176901756 1107 26-27 0.24224459596045733 1107 28-29 0.2965688954849881 1107 30-31 0.34162216017472957 1107 32-33 0.42059633073807134 1107 34-35 0.31106149214676293 1107 36-37 0.5353271690160923 1107 38-39 0.36385050696380006 1107 40-41 0.3059195746072163 1107 42-43 0.3874627986633641 1107 44-45 0.2394010969792646 1107 46-47 0.37571062987627357 1107 48-49 0.3798664358171209 1107 50-51 0.4255008385260055 1107 52-53 0.46755921265409484 1107 54-55 0.6442572805773423 1107 56-57 0.619155765867589 1107 58-59 0.6832656719172476 1107 60-61 0.5427759793496065 1107 62-63 0.40440925884946566 1107 64-65 0.15786104779195753 1107 66-67 0.4960837082356626 1107 68-69 0.43373531741213966 1107 70-71 0.23454006178466003 1107 72-73 0.4708813475404945 1107 74-75 0.25783908040748216 1107 76-77 0.05836274882852166 1107 78-79 0.3406383244101008 1107 80-81 0.715326789438052 1107 82-83 1.0097437824862503 1107 84-85 0.8578110746883105 1107 86-87 0.7921510510738727 1107 88-89 0.8000581561966946 1107 90-91 0.5841836923989199 1107 92-93 0.4297844784145681 1107 94-95 0.43907435708106846 1107 96-97 0.7438778923804001 1107 98-99 1.0803839528149197 1107 100-101 0.628011291600501 1108 1 0.03547695923881733 1108 2 0.25223429038772593 1108 3 -0.04224282027032089 1108 4 0.13676311015104403 1108 5 0.03694539934171104 1108 6 -0.05311011640608854 1108 7 0.0969025918722437 1108 8 0.027352263291781753 1108 9 0.08951265229674021 1108 10-11 -0.1567190811971031 1108 12-13 -0.01602143614066165 1108 14-15 -0.1537079483684849 1108 16-17 0.17142586690935246 1108 18-19 0.16967597208138585 1108 20-21 -0.07315827743480696 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-1.1875847212838515 2316 88-89 -0.8634835104699725 2316 90-91 -1.3499423238612458 2316 92-93 -1.426094586626082 2316 94-95 -1.1807173095855994 2316 96-97 -0.8596129612781382 2316 98-99 0.10634431866857952 2316 100-101 0.40385478753546167 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 12.0 5 25.0 6 73.0 7 142.0 8 331.0 9 613.0 10 991.0 11 1488.0 12 2281.0 13 3225.0 14 3917.0 15 5154.0 16 6684.0 17 8353.0 18 7367.0 19 5696.0 20 5378.0 21 5674.0 22 6196.0 23 6864.0 24 7985.0 25 9283.0 26 11472.0 27 13623.0 28 16826.0 29 20280.0 30 23969.0 31 29445.0 32 35606.0 33 44750.0 34 57042.0 35 73409.0 36 92242.0 37 89787.0 38 32152.0 39 771.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.031290933831855 19.382288606564675 14.899755827705924 21.68666463189755 2 16.01201206929991 22.071461955951506 38.21446853429226 23.70205744045632 3 21.487852915602286 24.707152179131146 29.581405256238863 24.22358964902771 4 13.253421881283648 17.2151902758328 37.22399632498454 32.307391517899006 5 13.303723580130733 38.69922679756848 33.541470610677315 14.45557901162347 6 31.40257568580575 37.3029371465267 16.227424600310915 15.067062567356631 7 28.901485106809112 33.25189592330789 19.95078785528196 17.895831114601034 8 26.50748916324516 33.8060654036105 18.806915812681112 20.879529620463227 9 25.818137311003447 18.157498187922023 20.38571828227724 35.63864621879729 10-11 25.76273934453851 26.368511907221905 26.651370021967402 21.217378726272184 12-13 27.70666365714322 23.836776800389117 26.925247252485242 21.53131228998242 14-15 22.978579330866364 25.750740726611475 26.92890931980315 24.341770622719007 16-17 21.576114126482395 28.938636112552786 27.31828358831744 22.16696617264738 18-19 21.39642235223148 28.19462073229953 29.537172518540363 20.871784396928632 20-21 22.699112477060865 25.946077835292847 29.533921306352383 21.82088838129391 22-23 21.86340132853716 26.28772573448649 29.63481918192757 22.214053755048774 24-25 22.974269307953666 26.38121162749111 29.66504401041149 20.979475054143734 26-27 22.40441892348348 26.029684674839555 30.59905819706332 20.96683820461364 28-29 21.13702762933268 27.84503285573044 29.968813283443062 21.049126231493815 30-31 21.854725710193744 26.323047051651827 29.76221037296547 22.06001686518896 32-33 21.851007010125738 26.814128344116106 29.724363763531453 21.610500882226706 34-35 22.845357547338512 26.178386218681077 29.64024717359773 21.336009060382683 36-37 22.7612023334552 25.834750679531403 30.190268792421037 21.21377819459236 38-39 22.01622376910935 26.700388028810462 30.290886489253303 20.99250171282689 40-41 21.859631163827224 26.94209634501855 29.67570265484475 21.522569836309476 42-43 21.68907292531821 27.215755779066207 28.786485509118148 22.30868578649743 44-45 22.4747071320761 26.546284616992576 28.630437599003628 22.348570651927695 46-47 23.06966622901414 27.42229548221785 28.132580104718702 21.375458184049315 48-49 23.147964905336764 27.359229119128404 27.998397707195295 21.494408268339537 50-51 22.009345014468956 27.7012361201738 28.858116802348732 21.43130206300851 52-53 22.759276788417935 28.116682204477595 27.546353144638342 21.57768786246613 54-55 21.667774044131907 27.42404397295244 28.220163595151277 22.68801838776438 56-57 22.917545989057853 27.320874577031024 27.914163827001815 21.84741560690931 58-59 23.663506329284953 27.03411781443158 27.42825260550994 21.874123250773525 60-61 22.211404187135937 27.313825369360835 29.09368798119834 21.38108246230489 62-63 22.000039738520535 27.363786286236564 28.78499473464603 21.85117924059687 64-65 21.68793534829453 28.582135579102648 28.00726123599971 21.722667836603115 66-67 22.143662708401056 27.517403678715258 27.62469887801013 22.71423473487356 68-69 21.85842381931099 27.74299280086091 27.841068950553407 22.557514429274697 70-71 22.794877969881725 27.60963714846845 27.65049003594895 21.94499484570087 72-73 22.598707505323134 26.886782330448494 28.498386964735765 22.016123199492604 74-75 21.826602624988176 28.716143446789268 27.508168811236278 21.94908511698628 76-77 22.344714782903107 27.343614018885454 27.448683335227546 22.86298786298389 78-79 22.338998246637534 27.711552003967714 27.607590167817293 22.341859581577452 80-81 22.54886142993051 27.83029102663247 27.555931047583016 22.064916495854 82-83 22.375724858843277 27.407294368991302 28.10314296251081 22.11383780965461 84-85 21.702342083427382 28.011057100801466 27.80862441145895 22.477976404312205 86-87 21.845198403772958 28.15213909842261 27.191891382805988 22.810771114998445 88-89 21.681086760589352 27.952198302651887 26.888931630792612 23.477783305966152 90-91 22.196231226075565 27.388628184095737 27.22004438340137 23.195096206427326 92-93 22.441471199760926 28.01815950608178 27.27899893815213 22.261370356005163 94-95 22.03519419115432 27.971998515361797 27.082248797705326 22.910558495778556 96-97 21.390002447887028 27.947160610000733 27.780100267995945 22.8827366741163 98-99 21.897591510519657 28.478814448393596 26.400554435804345 23.2230396052824 100-101 21.49148463355471 28.167789438211532 26.559454965681024 23.781270962552732 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 139.0 1 109.5 2 177.5 3 243.0 4 189.5 5 186.0 6 280.5 7 522.0 8 784.0 9 907.0 10 872.0 11 794.0 12 793.0 13 873.5 14 952.0 15 1050.5 16 1159.5 17 1176.0 18 1242.0 19 1386.5 20 1481.0 21 1624.5 22 1850.5 23 2215.5 24 2699.0 25 3202.0 26 3913.0 27 4656.5 28 5426.5 29 6290.0 30 7275.5 31 8691.5 32 10115.5 33 11021.0 34 12036.5 35 13024.5 36 14137.5 37 15318.0 38 16135.5 39 16501.5 40 16549.5 41 17620.0 42 18894.0 43 19974.5 44 20936.5 45 21403.0 46 22056.0 47 25992.5 48 35906.0 49 35506.0 50 26147.0 51 22411.0 52 20675.5 53 19783.5 54 18863.5 55 17210.5 56 15255.5 57 13657.0 58 12507.0 59 11048.5 60 9315.0 61 7791.5 62 6454.0 63 5192.0 64 3935.0 65 2908.0 66 2197.5 67 1874.5 68 1420.5 69 920.5 70 691.0 71 552.0 72 490.0 73 413.0 74 326.5 75 263.5 76 180.5 77 123.0 78 88.0 79 63.0 80 42.5 81 34.5 82 22.5 83 12.5 84 8.5 85 7.0 86 4.0 87 1.5 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007947685157221107 2 0.012716296251553773 3 0.005245472203765931 4 1.5895370314442214E-4 5 0.00667605553206573 6 0.0 7 1.5895370314442214E-4 8 7.947685157221108E-4 9 3.179074062888443E-4 10-11 0.0 12-13 0.0 14-15 3.179074062888443E-4 16-17 0.0035764583207494984 18-19 0.002145874992449699 20-21 5.563379610054775E-4 22-23 1.5895370314442214E-4 24-25 2.3843055471663323E-4 26-27 2.3843055471663323E-4 28-29 0.0 30-31 0.0011921527735831662 32-33 0.0038148888754661317 34-35 2.3843055471663323E-4 36-37 6.358148125776886E-4 38-39 0.00588128701634362 40-41 0.0 42-43 0.0018279675861608547 44-45 0.005324949055338142 46-47 0.0 48-49 0.0024637823987385433 50-51 0.0013511064767275883 52-53 5.563379610054775E-4 54-55 0.0 56-57 0.01247786569683714 58-59 0.0023048286955941215 60-61 0.003258550914460654 62-63 2.3843055471663323E-4 64-65 0.003179074062888443 66-67 0.0013511064767275883 68-69 0.0017484907345886437 70-71 0.00437122683647161 72-73 0.003258550914460654 74-75 0.007073439789926786 76-77 8.742453672943219E-4 78-79 0.00588128701634362 80-81 0.002145874992449699 82-83 0.004132796281754976 84-85 0.0025432592503107543 86-87 8.742453672943219E-4 88-89 0.0023843055471663324 90-91 0.007947685157221107 92-93 0.0034969814691772874 94-95 7.152916641498996E-4 96-97 0.0 98-99 9.537222188665329E-4 100-101 0.0025432592503107543 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 629114.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.16536478702236 #Duplication Level Percentage of deduplicated Percentage of total 1 86.37982668641779 56.28972916260183 2 8.253849531211634 10.75730231197199 3 2.163378421589537 4.229320320457644 4 0.9270226196282699 2.416390686955891 5 0.5171309058007902 1.6849512059575893 6 0.35087244009882995 1.371883833165174 7 0.23687798092125134 1.0805368025722666 8 0.19029337399754895 0.9920429706482841 9 0.13296438070607228 0.7798205135152545 >10 0.7634091350590413 9.15457011890936 >50 0.051358433524309965 2.3207598534966998 >100 0.02739112738547723 3.3397742003931663 >500 0.001956509098951438 0.7914353254008202 >1k 0.003423890923165017 3.5130102452296175 >5k 2.4456363736892977E-4 1.278472448724402 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8022 1.2751266066245543 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2946 0.46827760946346764 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2572 0.4088289244874537 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1905 0.3028068044901242 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1669 0.2652937305480406 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1665 0.2646579157354629 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1481 0.23541043435688921 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1323 0.2102957492600705 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1319 0.20965993444749284 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1245 0.1978973604148056 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1210 0.1923339808047508 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1201 0.19090339747645102 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1191 0.18931386044500678 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1184 0.18820118452299583 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1132 0.17993559195948589 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 932 0.14814485133060146 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 724 0.11508248107656165 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.947685157221107E-5 0.0 12-13 0.0 0.0 0.0 1.5895370314442214E-4 0.0 14-15 0.0 0.0 0.0 1.5895370314442214E-4 0.0 16-17 0.0 0.0 0.0 1.5895370314442214E-4 0.0 18-19 0.0 0.0 0.0 1.5895370314442214E-4 0.0 20-21 0.0 0.0 0.0 2.3843055471663323E-4 0.0 22-23 0.0 0.0 0.0 5.563379610054775E-4 0.0 24-25 0.0 0.0 0.0 6.358148125776886E-4 0.0 26-27 0.0 0.0 0.0 7.947685157221108E-4 0.0 28-29 0.0 0.0 0.0 8.742453672943219E-4 0.0 30-31 0.0 0.0 0.0 0.0026227361018829652 0.0 32-33 0.0 0.0 0.0 0.0069144860867823635 0.0 34-35 0.0 0.0 0.0 0.01319315736098704 0.0 36-37 0.0 0.0 0.0 0.022332995291791315 0.0 38-39 0.0 0.0 0.0 0.03894365727038343 0.0 40-41 0.0 0.0 0.0 0.058415485905575146 0.0 42-43 0.0 0.0 0.0 0.0896498885734541 0.0 44-45 0.0 0.0 0.0 0.12859354584383753 0.0 46-47 0.0 0.0 0.0 0.17627965678716417 0.0 48-49 0.0 0.0 0.0 0.23286717510657845 0.0 50-51 0.0 0.0 0.0 0.2996277304272358 0.0 52-53 0.0 0.0 0.0 0.3663088088963209 0.0 54-55 0.0 0.0 0.0 0.43934803549118284 0.0 56-57 0.0 0.0 0.0 0.5415552666130463 0.0 58-59 0.0 0.0 0.0 0.6614858356355129 0.0 60-61 0.0 0.0 0.0 0.7924636870265167 0.0 62-63 0.0 0.0 0.0 0.9433107513105733 0.0 64-65 0.0 0.0 0.0 1.0853358850701145 0.0 66-67 0.0 0.0 0.0 1.265509907584317 0.0 68-69 0.0 0.0 0.0 1.4754877494380987 0.0 70-71 0.0 0.0 0.0 1.7093086467635437 0.0 72-73 0.0 0.0 0.0 1.9722975486159902 1.5895370314442214E-4 74-75 0.0 0.0 0.0 2.2468900707979795 1.5895370314442214E-4 76-77 0.0 0.0 0.0 2.5301455698013395 1.5895370314442214E-4 78-79 0.0 0.0 0.0 2.8648225917719206 1.5895370314442214E-4 80-81 0.0 0.0 0.0 3.277227974580124 1.5895370314442214E-4 82-83 0.0 0.0 0.0 3.6595911074940313 1.5895370314442214E-4 84-85 0.0 0.0 0.0 4.097270129102197 1.5895370314442214E-4 86-87 0.0 0.0 0.0 4.568011520964404 1.5895370314442214E-4 88-89 0.0 0.0 0.0 5.092558741340997 1.5895370314442214E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 85 3.6379788E-12 33.5292 94-95 TACCGTG 75 3.077706E-4 31.665209 7 TACCTGG 590 0.0 28.99324 2 GTACACG 140 5.785314E-7 27.15024 1 GATCGAT 35 0.008390497 27.143766 30-31 GTACCTG 690 0.0 26.85513 1 TAAGGTG 305 0.0 24.918867 5 GTAGGGG 150 3.027754E-5 22.169172 3 GTACATG 2760 0.0 20.657791 1 GTGTAGG 230 8.97835E-8 20.657791 1 AGTACTT 3115 0.0 20.586563 12-13 GAGTACT 3055 0.0 20.291187 12-13 GTAAGGT 235 1.1294287E-7 20.213442 4 CTGGACG 235 1.1294287E-7 20.213442 4 GGTGTGC 570 0.0 19.999079 8 GTACTAT 170 7.929961E-5 19.564144 1 CTACACT 365 5.456968E-12 19.5212 4 TACACTA 220 1.3423396E-6 19.432468 5 TACATGG 2870 0.0 19.205357 2 GTACTTT 3220 0.0 19.17766 14-15 >>END_MODULE