FastQCFastQC Report
Thu 26 May 2016
SRR936398_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936398_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences710664
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT47320.6658561570587507No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT42040.5915594429997861No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36990.5204991388335416No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28420.39990769196132064No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC27540.3875249062848266No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT27120.3816149403937726No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18590.2615863474159377No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT16620.2338657931174226No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT13390.18841534114574537No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC11420.1606947868472302No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC10450.1470455799083674No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC9670.13606992896783854No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA9270.13044139002397756No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG8760.12326500287055486No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA7550.10623867256537547No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7390.10398725698783111No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA7240.10187655488388325No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA7110.10004727972712843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCG1151.0319491E-733.056215
TACCTGG7300.028.641172
GTACCTG7650.027.953911
GTACATG32200.027.1552281
GTACACG1257.359611E-626.612121
TACATGG32800.025.787372
ACATGGG31300.025.6568473
CATGGGG14000.024.098574
ACGTCTA1201.7054596E-423.7591515
GAGTACT30900.022.67949112-13
GTATAGT2154.3943146E-822.103091
GTACTTT32450.021.37581314-15
AGTACTT32050.021.2727512-13
GTCCTAC1604.968377E-520.790721
GGACCGT1200.004751140719.7992946
TAGGGGT2201.3383215E-619.4393064
GACGTAT1101.3465906E-619.42973362-63
GTGTAGC3254.838512E-1019.008661
CCTACGT1507.69712E-419.007322
GGGCGTA1759.911362E-519.0046469
Produced by FastQC (version 0.11.5)