##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936398_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 710664 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.75071060304166 31.0 31.0 34.0 30.0 34.0 2 31.81942380646832 33.0 31.0 34.0 30.0 34.0 3 31.86053043351007 33.0 31.0 34.0 30.0 34.0 4 35.49623028604235 37.0 35.0 37.0 33.0 37.0 5 35.462947328132564 37.0 35.0 37.0 33.0 37.0 6 35.57380562403611 37.0 35.0 37.0 33.0 37.0 7 35.432602467551476 37.0 35.0 37.0 33.0 37.0 8 35.42797862280909 37.0 35.0 37.0 33.0 37.0 9 37.02627964832889 39.0 37.0 39.0 33.0 39.0 10-11 36.892686980063715 39.0 37.0 39.0 33.0 39.0 12-13 36.817593124176824 39.0 37.0 39.0 33.0 39.0 14-15 37.954954802832276 40.0 38.0 41.0 33.0 41.0 16-17 37.92668687312147 40.0 38.0 41.0 33.0 41.0 18-19 37.90591967512073 40.0 38.0 41.0 33.0 41.0 20-21 37.80639444238065 40.0 37.5 41.0 33.0 41.0 22-23 37.70529181160154 40.0 37.0 41.0 32.5 41.0 24-25 37.55298987988698 40.0 37.0 41.0 32.0 41.0 26-27 37.37158277329371 39.5 37.0 41.0 32.0 41.0 28-29 37.46957422917159 40.0 37.0 41.0 32.0 41.0 30-31 37.40487065617507 39.5 37.0 41.0 32.0 41.0 32-33 37.22482847027568 39.0 36.0 41.0 32.0 41.0 34-35 37.029490729796365 39.0 36.0 41.0 31.0 41.0 36-37 36.783863400988366 39.0 35.5 41.0 30.5 41.0 38-39 36.60519528215866 39.0 35.0 40.5 30.0 41.0 40-41 36.4722133103689 39.0 35.0 40.0 30.0 41.0 42-43 36.20465508313352 39.0 35.0 40.0 30.0 41.0 44-45 35.91193025114541 38.5 35.0 40.0 29.5 41.0 46-47 35.53994363018248 38.0 35.0 40.0 27.5 41.0 48-49 35.272419877748135 38.5 35.0 40.0 27.0 41.0 50-51 34.557243648193804 38.0 34.0 39.5 25.0 40.5 52-53 34.7428383877613 38.0 34.0 39.5 26.0 40.5 54-55 35.11375417919017 38.5 35.0 40.0 26.5 41.0 56-57 35.009564294800356 38.0 34.5 40.0 26.0 41.0 58-59 34.78445861898169 38.0 34.0 40.0 26.0 41.0 60-61 34.453129749079736 38.0 34.0 40.0 25.0 41.0 62-63 34.171604302455165 37.5 34.0 40.0 24.5 41.0 64-65 33.86845682347776 37.0 33.5 40.0 23.5 41.0 66-67 33.47906324226357 36.5 33.0 39.0 22.5 41.0 68-69 33.08832092240496 36.0 33.0 39.0 22.0 41.0 70-71 32.64505800209382 35.5 32.0 39.0 20.5 40.0 72-73 32.22190374072698 35.0 32.0 38.0 20.0 40.0 74-75 31.819448431326194 35.0 31.5 37.0 18.5 39.5 76-77 31.4027669897448 35.0 31.0 37.0 17.5 39.0 78-79 30.93046643702228 35.0 31.0 36.0 12.5 39.0 80-81 30.490777638940482 34.5 30.5 36.0 7.5 37.5 82-83 30.076506619161798 34.0 30.0 35.0 7.0 37.0 84-85 29.699969324462757 34.0 30.0 35.0 3.5 37.0 86-87 29.406225726925804 34.0 29.5 35.0 2.0 36.0 88-89 29.011347134510824 34.0 29.0 35.0 2.0 36.0 90-91 28.64622240608783 34.0 29.0 35.0 2.0 35.5 92-93 28.259011290849124 34.0 29.0 35.0 2.0 35.0 94-95 27.857371416027828 34.0 27.5 35.0 2.0 35.0 96-97 27.283790229982102 34.0 26.5 35.0 2.0 35.0 98-99 26.668047769410016 33.0 25.5 35.0 2.0 35.0 100-101 24.621423063501176 31.0 20.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3064818824726423 1101 2 0.2019877427599539 1101 3 0.26694723643551654 1101 4 0.09054818613205384 1101 5 0.2817563399973295 1101 6 0.22866039668570437 1101 7 0.13931725800325268 1101 8 0.18487184475419838 1101 9 0.3379988680631669 1101 10-11 0.19746866862614354 1101 12-13 0.3628107787392665 1101 14-15 0.24848579166702933 1101 16-17 0.3734010777917902 1101 18-19 0.4192637745626371 1101 20-21 0.41139560142350007 1101 22-23 0.4516597066808856 1101 24-25 0.3712296599093534 1101 26-27 0.4492058107567942 1101 28-29 0.43705189581125836 1101 30-31 0.29745918006453564 1101 32-33 0.2649804189854237 1101 34-35 0.2988086424716627 1101 36-37 0.5270356177840725 1101 38-39 0.24835384523331072 1101 40-41 0.06798756457303057 1101 42-43 0.14383962139500994 1101 44-45 0.05467414011616256 1101 46-47 0.12661524055281603 1101 48-49 0.12431186236044311 1101 50-51 0.24921356882223478 1101 52-53 0.21266496104370702 1101 54-55 0.34317520262543866 1101 56-57 -0.010097121878466453 1101 58-59 0.2220976171402782 1101 60-61 0.051235014395558665 1101 62-63 0.13821713278346692 1101 64-65 0.016153459060014086 1101 66-67 0.02974118660948477 1101 68-69 0.178708516717478 1101 70-71 0.13139544621365218 1101 72-73 0.16987523840754903 1101 74-75 0.048683567740610556 1101 76-77 0.17836122215814498 1101 78-79 0.04538030510385127 1101 80-81 0.0855880227125283 1101 82-83 0.1889226537744939 1101 84-85 0.04407988244449612 1101 86-87 0.011477393372480549 1101 88-89 0.015563850762529086 1101 90-91 0.019338516709179743 1101 92-93 -0.05335006927997554 1101 94-95 -0.03338436423212343 1101 96-97 -0.02614210461008426 1101 98-99 -0.3308475538115836 1101 100-101 0.0632364855985017 1102 1 0.07127125879126694 1102 2 0.10080226595168895 1102 3 0.08383627173997255 1102 4 0.09443182272499939 1102 5 0.19470721340784536 1102 6 0.1929569251690566 1102 7 -6.638130737144365E-4 1102 8 0.04284892890635916 1102 9 0.2877567659415732 1102 10-11 0.20818781005107212 1102 12-13 0.34403923431418804 1102 14-15 0.18897357294392236 1102 16-17 0.12646832983078582 1102 18-19 0.19080493790315245 1102 20-21 0.005278873789606564 1102 22-23 -0.24858948970968697 1102 24-25 -0.07788051218511072 1102 26-27 -0.14099034442123326 1102 28-29 -0.05926968495495544 1102 30-31 -0.09101510633627186 1102 32-33 -0.16482545898336554 1102 34-35 -0.25400343333318887 1102 36-37 -0.2557123160440611 1102 38-39 -0.3174143771168403 1102 40-41 -0.520899276672445 1102 42-43 -0.45157992714855055 1102 44-45 -0.20931121905226746 1102 46-47 -0.13138755692673243 1102 48-49 -0.104910763410615 1102 50-51 -0.07039412082171026 1102 52-53 -0.13004313615376617 1102 54-55 -0.300045312804464 1102 56-57 0.04426482065741055 1102 58-59 -0.16770147642416333 1102 60-61 -0.6527798525828246 1102 62-63 -0.3330236249605676 1102 64-65 -0.09136575934181934 1102 66-67 -0.17641310531452348 1102 68-69 -0.16030974518118768 1102 70-71 -0.018114777416194272 1102 72-73 -0.030465286503122968 1102 74-75 -0.12395750628278535 1102 76-77 -0.12028439763361476 1102 78-79 -0.01617620468977421 1102 80-81 -0.05251102850374778 1102 82-83 0.011543640959690293 1102 84-85 -0.18801089375551427 1102 86-87 -0.3420721602321919 1102 88-89 -0.3556800599899361 1102 90-91 -0.6639542895933594 1102 92-93 -0.6368762525687082 1102 94-95 -0.36813736679312825 1102 96-97 -0.6045169244672977 1102 98-99 -0.6217742827882198 1102 100-101 -0.20510464599447786 1103 1 0.019450545523710616 1103 2 0.25043461494912833 1103 3 0.09352335350659047 1103 4 0.039531839067848296 1103 5 0.12218219275843722 1103 6 -0.15994828441378672 1103 7 -0.1325651174097402 1103 8 -0.10448532526343257 1103 9 0.07035775244389697 1103 10-11 -0.049552485041459704 1103 12-13 0.22410733162165997 1103 14-15 0.3441015992893526 1103 16-17 0.39076942694706673 1103 18-19 0.23511551260983765 1103 20-21 0.16475794705542057 1103 22-23 -0.03585512915362443 1103 24-25 0.37870272181861964 1103 26-27 0.07192795206076852 1103 28-29 0.1787478201924131 1103 30-31 0.06297523055988563 1103 32-33 0.28164445408403793 1103 34-35 0.2878178541425811 1103 36-37 0.3741290952208871 1103 38-39 0.1667690262467545 1103 40-41 0.09095553572868198 1103 42-43 0.3235257492466346 1103 44-45 0.41482817850867093 1103 46-47 0.45714935424956593 1103 48-49 0.5826227977971357 1103 50-51 -0.054639992990338726 1103 52-53 0.14198547834492814 1103 54-55 0.16763275957227108 1103 56-57 0.17157214383125563 1103 58-59 0.25819947833895895 1103 60-61 0.3137277184470477 1103 62-63 0.03178883295974799 1103 64-65 0.0862605017866187 1103 66-67 0.07767150268741574 1103 68-69 -0.05156196708299632 1103 70-71 0.13817838679284478 1103 72-73 0.2095572148866509 1103 74-75 0.0527462357248325 1103 76-77 -0.12768127204796897 1103 78-79 0.1276673683744356 1103 80-81 0.2412437946874526 1103 82-83 0.027851469562957476 1103 84-85 -0.2546569057533077 1103 86-87 -0.3741410576451649 1103 88-89 -0.37582719611072335 1103 90-91 -0.2079503117817758 1103 92-93 -0.16174199196273165 1103 94-95 -0.21953291358762428 1103 96-97 -0.12617214588135184 1103 98-99 -0.11135555880505521 1103 100-101 -0.3181974765317541 1104 1 0.20923146828302563 1104 2 0.12905251327446976 1104 3 0.27914819222064935 1104 4 0.020680059150159025 1104 5 0.1681028468663044 1104 6 0.058898062714305865 1104 7 0.15227604140427786 1104 8 0.2050206983420395 1104 9 0.29144512528266375 1104 10-11 0.4412117726357394 1104 12-13 0.2810022207248437 1104 14-15 0.302391357122751 1104 16-17 0.22462113895488756 1104 18-19 0.29863840502702743 1104 20-21 0.15159701424544636 1104 22-23 0.15761329954780479 1104 24-25 0.20378726960679217 1104 26-27 0.28168116759310635 1104 28-29 0.20114449532517398 1104 30-31 -0.009219752258914582 1104 32-33 0.03805854130747832 1104 34-35 0.108590125184449 1104 36-37 0.07827295548954538 1104 38-39 0.16565530437841858 1104 40-41 0.07868422221598337 1104 42-43 0.33107053580591383 1104 44-45 0.13728733072185406 1104 46-47 0.40300481488221607 1104 48-49 0.05395125067447992 1104 50-51 0.06835157260380242 1104 52-53 0.06916572472193394 1104 54-55 0.2777146588755173 1104 56-57 0.36777734029450215 1104 58-59 0.43688392833692546 1104 60-61 0.3794848455879034 1104 62-63 0.22843544357813528 1104 64-65 0.10562839671647595 1104 66-67 0.3665329258066521 1104 68-69 0.2804697604082733 1104 70-71 0.36103820723914737 1104 72-73 0.26081558477813616 1104 74-75 0.0488074632636426 1104 76-77 0.281781289240687 1104 78-79 0.27988734202540755 1104 80-81 0.3791144128030908 1104 82-83 0.3459413007390211 1104 84-85 0.46435242506283103 1104 86-87 0.2885370359889663 1104 88-89 0.3282080083658947 1104 90-91 0.37566032018747464 1104 92-93 0.0012892329955001003 1104 94-95 0.15896274697167811 1104 96-97 0.1590968451137975 1104 98-99 0.015812463795349174 1104 100-101 0.04863126835802589 1105 1 0.11855128720635122 1105 2 0.1863245538176983 1105 3 0.05866304132158717 1105 4 0.14248128164861384 1105 5 0.4080800198578629 1105 6 0.23653309227616148 1105 7 0.4510118693616505 1105 8 0.06196484804146962 1105 9 0.2722378910122458 1105 10-11 0.24778091505054078 1105 12-13 0.2790405934098459 1105 14-15 0.16924975160117128 1105 16-17 0.14093640237262406 1105 18-19 0.07845288610293721 1105 20-21 -0.1930044972191851 1105 22-23 -0.1596942130229948 1105 24-25 -0.3406132410320666 1105 26-27 -0.21239931410514856 1105 28-29 0.005995564489033711 1105 30-31 -0.17990386978502215 1105 32-33 -0.3667393088193549 1105 34-35 -0.5357687118696361 1105 36-37 -0.34053942431405204 1105 38-39 -0.5209439441775743 1105 40-41 -0.6350446399852032 1105 42-43 -0.4135330648357822 1105 44-45 -0.3401000763784836 1105 46-47 -0.09717221538556231 1105 48-49 -0.01189521240371505 1105 50-51 -0.1613276067674434 1105 52-53 0.01914257883910153 1105 54-55 0.10251187125356154 1105 56-57 0.24225683282239885 1105 58-59 0.0626639335928516 1105 60-61 0.07040319285092522 1105 62-63 -0.2615612467255488 1105 64-65 -0.1207091989046134 1105 66-67 -0.1664678251975289 1105 68-69 0.03355157148289578 1105 70-71 0.1308664219053881 1105 72-73 0.17069563302712965 1105 74-75 0.040117036422778085 1105 76-77 0.09116092460231684 1105 78-79 0.14712673642795338 1105 80-81 0.2847457346254245 1105 82-83 0.5942443941505253 1105 84-85 0.3145105977586091 1105 86-87 0.6523199419023271 1105 88-89 0.6611497126285855 1105 90-91 0.4888931677688113 1105 92-93 0.4854571192650994 1105 94-95 0.7812719693689765 1105 96-97 0.7064584893621308 1105 98-99 0.603133468051773 1105 100-101 0.8911892347750552 1106 1 0.08038870295614231 1106 2 -0.08480441392693194 1106 3 -0.05158459012067951 1106 4 0.10423511880168945 1106 5 0.10341514018119113 1106 6 -0.06757807919726844 1106 7 0.296734734674736 1106 8 0.3600225411528015 1106 9 0.2120389142530641 1106 10-11 0.3343571476856013 1106 12-13 0.4353407778797447 1106 14-15 0.11540349442982745 1106 16-17 0.17477752543016578 1106 18-19 0.1140872178025063 1106 20-21 0.16765281859447612 1106 22-23 0.1270579824076492 1106 24-25 0.0014769402737897508 1106 26-27 0.18413336811111236 1106 28-29 -0.10269019192711681 1106 30-31 -0.1759316477286319 1106 32-33 0.049101521713097895 1106 34-35 -0.20750556465787895 1106 36-37 -0.2323126039423684 1106 38-39 -0.16912282625414576 1106 40-41 -0.14793290734119324 1106 42-43 -0.28380788917149147 1106 44-45 -0.2178424990832113 1106 46-47 -0.32387169660127313 1106 48-49 -0.5979550487090606 1106 50-51 -0.3580761008602238 1106 52-53 -0.4321848283737282 1106 54-55 -0.0844027054506924 1106 56-57 -0.1550608912130116 1106 58-59 -0.2979829348962255 1106 60-61 -0.2948822877558612 1106 62-63 -0.02690239664764249 1106 64-65 -0.12978941644775688 1106 66-67 -0.3059030428302236 1106 68-69 -0.49760533539264173 1106 70-71 -0.31478361063056326 1106 72-73 0.03755716717196833 1106 74-75 -0.11267135451014099 1106 76-77 -0.3519966747815886 1106 78-79 -0.36012146812469226 1106 80-81 -0.23944206051149663 1106 82-83 -0.19775982059870856 1106 84-85 -0.14889839410441752 1106 86-87 0.1402980562497831 1106 88-89 -0.048413917548064234 1106 90-91 0.30534636076970045 1106 92-93 0.1392633781080157 1106 94-95 0.23469406516198887 1106 96-97 0.47996049226350124 1106 98-99 0.4613334238745139 1106 100-101 1.0184003364006564 1107 1 0.13160679325065772 1107 2 0.0499534815179814 1107 3 0.10706144057541778 1107 4 0.2889778792084243 1107 5 0.23036447704230056 1107 6 0.2593527592838498 1107 7 0.46551283259282883 1107 8 0.5538939718063034 1107 9 0.492174456928268 1107 10-11 0.526876234650409 1107 12-13 0.4241955039355503 1107 14-15 0.5978790821738116 1107 16-17 0.22243748036138555 1107 18-19 0.4574507838417219 1107 20-21 0.7839866078474813 1107 22-23 0.7011243747750981 1107 24-25 0.7124145278608154 1107 26-27 0.632646210471286 1107 28-29 0.504503715939741 1107 30-31 0.3545612500145694 1107 32-33 0.5219397337605827 1107 34-35 0.4569099986458198 1107 36-37 0.6241091366991327 1107 38-39 0.6737642663610757 1107 40-41 0.29247007706410955 1107 42-43 0.6545462595106457 1107 44-45 0.7713051179891082 1107 46-47 0.6027037487612432 1107 48-49 0.9994010843447398 1107 50-51 0.7410051462669145 1107 52-53 0.7344351252121513 1107 54-55 0.6229753453806097 1107 56-57 0.417811514809614 1107 58-59 0.4961875529004516 1107 60-61 0.22039989662253845 1107 62-63 0.34628280016390534 1107 64-65 0.45655317354965774 1107 66-67 0.3848446841112647 1107 68-69 0.8385943125775768 1107 70-71 0.847312648212224 1107 72-73 1.0360422619621517 1107 74-75 0.6343366726656612 1107 76-77 0.9039143984607882 1107 78-79 0.9718613900431805 1107 80-81 1.089513790021595 1107 82-83 0.7567313618401634 1107 84-85 0.7000399109955282 1107 86-87 0.7113346381976093 1107 88-89 0.807855071319274 1107 90-91 1.0114869820910464 1107 92-93 0.728948289035511 1107 94-95 0.8420617456893602 1107 96-97 0.7943432629844907 1107 98-99 0.8914936310563668 1107 100-101 1.1221229952344736 1108 1 -0.08167177826149441 1108 2 -0.039810660410303456 1108 3 -0.06468551612607953 1108 4 0.23015182599897344 1108 5 0.29625028070322657 1108 6 0.29687084745661707 1108 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56-57 -0.8465257586211408 2316 58-59 -0.9591741595065599 2316 60-61 -1.5901519763226233 2316 62-63 -0.9931913258018383 2316 64-65 -0.9402966591975073 2316 66-67 -1.6099371092813257 2316 68-69 -1.173238262624782 2316 70-71 -1.1752593196392027 2316 72-73 -0.9600865616178353 2316 74-75 -1.3619293105230739 2316 76-77 -1.9317520129934742 2316 78-79 -1.6609295828074053 2316 80-81 -1.5964152718476292 2316 82-83 -1.448375479353576 2316 84-85 -1.478028062849976 2316 86-87 -1.7566191451557174 2316 88-89 -2.1780563849882277 2316 90-91 -2.107323946459033 2316 92-93 -2.709033049069067 2316 94-95 -2.883743913475154 2316 96-97 -2.9704013949558252 2316 98-99 -3.2120270718559176 2316 100-101 -3.373696707436249 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1499.0 3 525.0 4 530.0 5 564.0 6 599.0 7 655.0 8 802.0 9 986.0 10 1286.0 11 1661.0 12 2288.0 13 2935.0 14 3245.0 15 4204.0 16 5437.0 17 7170.0 18 6426.0 19 3964.0 20 3870.0 21 4107.0 22 4426.0 23 4875.0 24 5562.0 25 6338.0 26 7570.0 27 9072.0 28 11074.0 29 13555.0 30 16839.0 31 20984.0 32 27719.0 33 36890.0 34 50969.0 35 71714.0 36 102769.0 37 136576.0 38 111814.0 39 19147.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.61710152269976 17.852036364659853 14.233020912494018 22.297841200146358 2 15.19494646307296 19.934959212305685 40.37214813313801 24.497946191483344 3 20.512968453819187 24.110305385943605 29.72886636673614 25.647859793501066 4 12.168538345246574 15.822886373297823 38.10496414766601 33.90361113378959 5 13.219584987470789 37.49598783681054 34.31033026438044 14.974096911338233 6 31.187520767744807 35.73023614685823 17.68200992346206 15.400233161934906 7 28.346202893328567 32.823711894513714 20.643187109233146 18.186898102924577 8 26.041094849209095 33.1656940491573 19.837456049230493 20.95575505240311 9 25.862428084081106 17.399571037728414 21.256150148053905 35.48185073013657 10-11 25.02709962243539 25.799474056158868 27.106168577952594 22.067257743453155 12-13 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30.535862544502766 21.870312258137144 38-39 21.859412983595483 26.072098251193488 30.241230461810158 21.827258303400868 40-41 21.730087835574956 26.388384652836994 30.15889767901318 21.722629832574867 42-43 21.360918019868855 26.555033349280947 29.126023696282328 22.958024934567867 44-45 21.799794133146463 26.1871643494333 29.08041682761008 22.932624689810154 46-47 22.408877656415786 26.642186317084082 28.85519772373557 22.093738302764557 48-49 22.37129272242962 26.59002931065779 28.628266843778277 22.410411123134313 50-51 21.4745202113835 26.969548365126926 29.414347257410984 22.14158416607859 52-53 22.093663834317045 27.412744658651096 28.152129327940212 22.341462179091646 54-55 21.359288877381445 26.602368813946647 28.879998761698783 23.15834354697313 56-57 22.24938823698271 26.76060400956306 28.26872825057588 22.721279502878346 58-59 22.945393908265142 26.424254364054683 28.190843400620576 22.439508327059603 60-61 22.17140066966249 26.399392674238538 29.57779180236865 21.85141485373032 62-63 21.785168763205238 26.81081058262407 29.26833043577373 22.13569021839696 64-65 21.376666798002937 27.586316073871025 28.73935472613573 22.297662401990305 66-67 21.524790510163157 27.092119242107632 28.029845705722185 23.35324454200702 68-69 21.28859581725614 27.0024372602493 28.68030519226494 23.028661730229626 70-71 22.114842016236732 26.846404010070206 28.092233303452947 22.946520670240115 72-73 21.87406994113057 26.29838856637076 29.159962034249386 22.667579458249286 74-75 21.394789573518402 27.704417914816254 28.37267748490119 22.528115026764155 76-77 21.648062871797396 26.870165094754466 28.210693693471846 23.271078339976288 78-79 21.83171400318822 26.82962019347506 28.469900165745273 22.86876563759145 80-81 22.15368787134339 27.08569812029784 28.0202361256644 22.740377882694368 82-83 21.88772835849249 26.786182926014135 28.70254734272155 22.62354137277182 84-85 21.656587404616406 27.217244157370175 28.296376039149052 22.829792398864367 86-87 21.681158767662467 27.40210267978391 28.21358059263992 22.703157959913707 88-89 21.488251663131084 27.225886798028515 27.67724527093821 23.608616267902185 90-91 21.619581102541712 26.794900391005672 28.054322839670785 23.531195666781827 92-93 22.144030240383465 27.16786311891952 28.087684164751547 22.60042247594547 94-95 21.761165151008775 26.90738949182952 28.088723153506535 23.24272220365517 96-97 21.045136423857834 27.00354401594631 28.69140183877929 23.259917721416564 98-99 21.498361707877315 27.550612529838308 27.521196459937848 23.42982930234653 100-101 21.35745064833233 26.898154600266626 27.932769394716207 23.81162535668484 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 84.0 1 75.0 2 198.5 3 298.0 4 211.0 5 182.0 6 329.5 7 689.5 8 1104.0 9 1283.0 10 1125.0 11 947.0 12 949.0 13 986.5 14 1088.0 15 1168.5 16 1198.5 17 1232.0 18 1260.5 19 1339.0 20 1501.5 21 1710.0 22 1931.5 23 2270.0 24 2821.5 25 3554.5 26 4351.5 27 5172.0 28 5883.0 29 6776.5 30 8123.5 31 9561.5 32 11136.5 33 12133.5 34 13369.0 35 14727.5 36 16021.5 37 17357.5 38 18064.5 39 18408.0 40 18841.5 41 20104.0 42 21318.5 43 22302.0 44 23066.5 45 24084.0 46 24983.5 47 29196.5 48 39761.5 49 39293.0 50 29610.5 51 25497.5 52 23380.0 53 22653.5 54 21889.0 55 20000.5 56 17994.0 57 15920.0 58 14259.5 59 12732.0 60 10797.0 61 9030.0 62 7641.5 63 6088.5 64 4533.0 65 3529.0 66 2702.0 67 2215.5 68 1653.0 69 1082.5 70 856.5 71 669.5 72 519.5 73 458.0 74 377.5 75 309.0 76 237.5 77 159.5 78 109.0 79 65.5 80 39.5 81 34.0 82 24.0 83 14.5 84 9.0 85 8.5 86 7.5 87 4.5 88 3.0 89 5.0 90 5.0 91 1.5 92 1.0 93 2.0 94 1.5 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011819931782108 2 0.004643544628685286 3 0.007739241047808811 4 0.03517836839913095 5 0.04559116544527372 6 0.060225366699312194 7 0.02842412166649781 8 0.02954982945527 9 0.015759909042810667 10-11 0.04530973849808067 12-13 0.04122904776378147 14-15 0.016885616831582857 16-17 0.021951301881057716 18-19 0.02765019756171693 20-21 0.022936296196233382 22-23 0.013227066518073238 24-25 0.0026735559983339526 26-27 0.0026735559983339526 28-29 0.0013367779991669763 30-31 0.004995328312676596 32-33 7.035673679826191E-4 34-35 0.0016885616831582857 36-37 0.004784258102281809 38-39 0.00513604178627312 40-41 0.0025328425247374284 42-43 5.628538943860953E-4 44-45 0.0014774914727635 46-47 0.003658550313509619 48-49 1.4071347359652383E-4 50-51 0.0017589184199565476 52-53 7.739241047808811E-4 54-55 0.0018292751567548094 56-57 0.0018292751567548094 58-59 0.0040806907342991906 60-61 0.0010553510519739286 62-63 0.0011960645255704523 64-65 0.0028142694719304764 66-67 0.0012664212623687142 68-69 0.004221404207895714 70-71 0.007457814100615762 72-73 0.0033067666295183095 74-75 0.0016885616831582857 76-77 0.0016182049463600236 78-79 0.03327873650557788 80-81 0.02145880472346988 82-83 0.003025339682325262 84-85 0.006543176522238358 86-87 0.006613533259036619 88-89 0.0044324744182905 90-91 0.00851316515258969 92-93 0.012804926097283667 94-95 0.003728907050307881 96-97 0.0041510474710974524 98-99 0.023639863564216 100-101 0.04263618249974671 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 710664.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.7239895943561 #Duplication Level Percentage of deduplicated Percentage of total 1 86.43750751735868 56.81017844632959 2 8.223546402267315 10.80968556342643 3 2.1948205077408818 4.327570806367231 4 0.9256216933693159 2.433422021732608 5 0.5034619628202641 1.6544764402776568 6 0.34332954787235864 1.353899257907873 7 0.24099402397264885 1.1087362106716263 8 0.16880628884503515 0.8875698217210367 9 0.1234177008105863 0.7300353315450894 >10 0.7544070806399038 9.165495286388472 >50 0.051993091139852975 2.329874378003087 >100 0.026745152636661988 3.048303211173462 >500 0.002995457094858748 1.4046916443057884 >1k 0.0023535734316747305 3.9360615801500667 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4732 0.6658561570587507 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4204 0.5915594429997861 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3699 0.5204991388335416 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2842 0.39990769196132064 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2754 0.3875249062848266 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2712 0.3816149403937726 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1859 0.2615863474159377 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1662 0.2338657931174226 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1339 0.18841534114574537 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1142 0.1606947868472302 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1045 0.1470455799083674 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 967 0.13606992896783854 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 927 0.13044139002397756 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 876 0.12326500287055486 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 755 0.10623867256537547 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 739 0.10398725698783111 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 724 0.10187655488388325 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 711 0.10004727972712843 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 1.407134735965238E-4 10-11 0.0 0.0 0.0 0.0 1.407134735965238E-4 12-13 0.0 0.0 0.0 1.407134735965238E-4 1.407134735965238E-4 14-15 0.0 0.0 0.0 1.407134735965238E-4 1.407134735965238E-4 16-17 0.0 0.0 0.0 2.814269471930476E-4 1.407134735965238E-4 18-19 0.0 0.0 0.0 2.814269471930476E-4 1.407134735965238E-4 20-21 0.0 0.0 0.0 2.814269471930476E-4 1.407134735965238E-4 22-23 0.0 0.0 0.0 4.221404207895714E-4 1.407134735965238E-4 24-25 0.0 0.0 0.0 5.628538943860952E-4 1.407134735965238E-4 26-27 0.0 0.0 0.0 0.0010553510519739286 1.407134735965238E-4 28-29 0.0 0.0 0.0 0.0020403453671495953 1.407134735965238E-4 30-31 0.0 0.0 0.0 0.0047139013654835474 1.407134735965238E-4 32-33 0.0 0.0 0.0 0.009146375783774047 1.407134735965238E-4 34-35 0.0 0.0 0.0 0.013086353044476716 1.407134735965238E-4 36-37 0.0 0.0 0.0 0.01998131325070638 1.407134735965238E-4 38-39 0.0 0.0 0.0 0.032997309558384834 1.407134735965238E-4 40-41 0.0 0.0 0.0 0.04833507818040593 1.407134735965238E-4 42-43 0.0 0.0 0.0 0.07106030416624454 1.407134735965238E-4 44-45 0.0 0.0 0.0 0.10159512793669019 1.407134735965238E-4 46-47 0.0 0.0 0.0 0.13916562538696206 1.407134735965238E-4 48-49 0.0 0.0 0.0 0.18292751567548096 1.407134735965238E-4 50-51 0.0 0.0 0.0 0.23471007395900173 1.407134735965238E-4 52-53 0.0 0.0 0.0 0.2919804577127869 1.407134735965238E-4 54-55 0.0 0.0 0.0 0.35206511093850257 1.407134735965238E-4 56-57 0.0 0.0 0.0 0.4364931950964169 1.407134735965238E-4 58-59 0.0 0.0 0.0 0.5326004975628427 1.407134735965238E-4 60-61 0.0 0.0 0.0 0.6422162934945347 1.407134735965238E-4 62-63 0.0 0.0 0.0 0.7687880629946079 1.407134735965238E-4 64-65 0.0 0.0 0.0 0.8876205914468722 1.407134735965238E-4 66-67 0.0 0.0 0.0 1.040153996825504 1.407134735965238E-4 68-69 0.0 0.0 0.0 1.225262571341731 1.407134735965238E-4 70-71 0.0 0.0 0.0 1.422472504587259 1.407134735965238E-4 72-73 0.0 0.0 0.0 1.6530315310751635 1.407134735965238E-4 74-75 0.0 0.0 0.0 1.8853494759830243 1.407134735965238E-4 76-77 0.0 0.0 0.0 2.1282209314106244 1.407134735965238E-4 78-79 0.0 0.0 0.0 2.4135174991275763 1.407134735965238E-4 80-81 0.0 0.0 0.0 2.7756436234282305 1.407134735965238E-4 82-83 0.0 0.0 0.0 3.123487330158837 1.407134735965238E-4 84-85 0.0 0.0 0.0 3.4974333862415996 1.407134735965238E-4 86-87 0.0 0.0 0.0 3.9393440500714823 1.407134735965238E-4 88-89 0.0 0.0 0.0 4.426564452399446 1.407134735965238E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGCG 115 1.0319491E-7 33.05621 5 TACCTGG 730 0.0 28.64117 2 GTACCTG 765 0.0 27.95391 1 GTACATG 3220 0.0 27.155228 1 GTACACG 125 7.359611E-6 26.61212 1 TACATGG 3280 0.0 25.78737 2 ACATGGG 3130 0.0 25.656847 3 CATGGGG 1400 0.0 24.09857 4 ACGTCTA 120 1.7054596E-4 23.759151 5 GAGTACT 3090 0.0 22.679491 12-13 GTATAGT 215 4.3943146E-8 22.10309 1 GTACTTT 3245 0.0 21.375813 14-15 AGTACTT 3205 0.0 21.27275 12-13 GTCCTAC 160 4.968377E-5 20.79072 1 GGACCGT 120 0.0047511407 19.799294 6 TAGGGGT 220 1.3383215E-6 19.439306 4 GACGTAT 110 1.3465906E-6 19.429733 62-63 GTGTAGC 325 4.838512E-10 19.00866 1 CCTACGT 150 7.69712E-4 19.00732 2 GGGCGTA 175 9.911362E-5 19.004646 9 >>END_MODULE