FastQCFastQC Report
Thu 26 May 2016
SRR936398_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936398_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences710664
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT84951.1953609582024698No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34010.4785665237017775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29250.41158691026983213No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21650.3046446703364741No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20540.2890254747672599No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18000.25328425247374287No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC15380.21641732239145364No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14500.20403453671495952No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT13790.19404388008960635No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA13550.19066675672328978No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG13290.18700820640978014No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT13250.18644535251539407No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA11900.16744903357986332No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC11770.16561975842310853No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA11410.16055407337363367No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA9590.13494422117906632No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8450.11890288518906261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACG1307.55972E-933.1916241
TACCTGG5200.026.4874632
GGTAAGG1901.1908924E-824.9972423
GTACATG33950.023.4421621
TACACGG1652.4300425E-623.027762
TAAGGTG3100.022.9797175
TACATGG34950.022.4224172
GTAAGGT2257.1502654E-821.1087824
GAGTACT37350.020.91509412-13
GTACCTG6650.020.9076751
CGTGAAC1150.003724280720.6498953
ACATGGG34850.020.4425073
CTGGTCG1404.855144E-420.3534649
ATTCGCG958.251336E-620.00060880-81
GTGTAAG2651.6641934E-819.9010581
GGACGCT1456.150353E-419.651626
GTACTTT39450.019.62118114-15
CTGGACG1951.0327209E-519.485034
AGTACTT38250.019.36768712-13
GCGACAG1759.9437646E-518.9965657
Produced by FastQC (version 0.11.5)