##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936398_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 710664 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.263547893237874 31.0 31.0 34.0 30.0 34.0 2 31.495999515945652 31.0 31.0 34.0 30.0 34.0 3 31.740983080611933 31.0 31.0 34.0 30.0 34.0 4 35.56993318924274 37.0 35.0 37.0 33.0 37.0 5 35.37558255378069 37.0 35.0 37.0 33.0 37.0 6 35.48735267299315 37.0 35.0 37.0 33.0 37.0 7 35.42932243648194 37.0 35.0 37.0 33.0 37.0 8 35.4004860243378 37.0 35.0 37.0 33.0 37.0 9 36.9899136582126 39.0 37.0 39.0 33.0 39.0 10-11 36.84736317021827 39.0 37.0 39.0 33.0 39.0 12-13 36.70925148874855 39.0 35.0 39.0 32.0 39.0 14-15 37.70587366181486 39.0 37.0 41.0 32.0 41.0 16-17 37.638362151452725 39.0 36.5 41.0 32.0 41.0 18-19 37.7243226053381 39.0 36.5 40.5 32.0 41.0 20-21 37.8072767158601 39.0 37.0 41.0 32.0 41.0 22-23 37.70608543559263 39.0 37.0 41.0 32.0 41.0 24-25 37.56612337194511 39.0 36.5 41.0 32.0 41.0 26-27 37.302272241171636 39.0 36.0 40.5 31.5 41.0 28-29 37.20097612936634 39.0 36.0 40.0 31.0 41.0 30-31 37.20233331081918 39.0 36.0 40.0 31.0 41.0 32-33 37.00205863811871 39.0 35.5 40.0 31.0 41.0 34-35 36.7885695631128 39.0 35.0 40.0 30.5 41.0 36-37 36.53322948678982 38.5 35.0 40.0 30.0 41.0 38-39 36.33285490752311 38.0 35.0 40.0 30.0 41.0 40-41 36.11563692546689 38.0 35.0 40.0 30.0 41.0 42-43 35.84710496099423 38.0 35.0 40.0 29.0 41.0 44-45 35.52771281505747 38.0 34.5 40.0 27.5 41.0 46-47 35.08339384012698 38.0 34.0 40.0 26.5 41.0 48-49 34.51442946314995 38.0 33.0 40.0 24.0 41.0 50-51 34.31000585368051 38.0 33.0 40.0 24.0 41.0 52-53 34.03956018596693 38.0 33.0 40.0 23.5 41.0 54-55 33.7578975437056 37.0 33.0 40.0 23.0 41.0 56-57 33.43716510193284 37.0 32.5 40.0 22.0 41.0 58-59 33.00539214030822 36.5 31.5 39.0 20.5 40.5 60-61 32.55247275787151 36.0 31.0 39.0 19.0 40.0 62-63 32.280310385780055 36.0 31.0 39.0 18.5 40.0 64-65 31.817479849830583 35.0 30.5 38.5 17.5 40.0 66-67 31.352513986919277 35.0 30.0 38.0 13.5 40.0 68-69 30.83854184255851 34.5 29.0 37.5 11.0 39.5 70-71 30.292389230353585 34.0 29.0 37.0 8.0 39.0 72-73 29.813285884750037 34.0 28.5 36.0 7.0 39.0 74-75 29.408533427892788 34.0 28.0 36.0 6.5 38.5 76-77 28.120396558711292 31.5 26.5 34.5 2.0 36.5 78-79 28.932005842423422 33.5 28.0 35.0 2.0 37.0 80-81 28.720130047392296 33.0 28.0 35.0 2.0 37.0 82-83 28.318523240237298 33.0 27.5 35.0 2.0 36.0 84-85 27.814040108968513 33.0 26.0 35.0 2.0 36.0 86-87 27.372331368973242 33.0 26.0 35.0 2.0 35.5 88-89 26.859465936082312 32.0 25.0 34.5 2.0 35.0 90-91 26.263140527731814 32.0 24.5 34.0 2.0 35.0 92-93 25.68585787376313 32.0 23.5 34.0 2.0 35.0 94-95 25.003786599574482 31.0 19.5 34.0 2.0 35.0 96-97 24.12857130795988 31.0 12.5 34.0 2.0 35.0 98-99 23.06290103339975 31.0 2.0 34.0 2.0 35.0 100-101 20.19484876115858 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.30851825101100516 1101 2 0.33033241584756823 1101 3 0.13154510599189706 1101 4 0.03791558581029619 1101 5 0.08118004834020098 1101 6 0.15415477261321087 1101 7 0.17373262509223508 1101 8 0.18268071047005208 1101 9 0.1523272262631039 1101 10-11 0.15500624151050602 1101 12-13 0.13143713395162848 1101 14-15 0.34901164558650777 1101 16-17 0.16161066279307335 1101 18-19 0.12319270611694577 1101 20-21 -0.07371871436745892 1101 22-23 0.04296778222524722 1101 24-25 -0.06172375912444039 1101 26-27 -0.010908050600669128 1101 28-29 -0.030055941542428855 1101 30-31 -0.09363210901643981 1101 32-33 0.1341251763036837 1101 34-35 0.18031705812602894 1101 36-37 0.1192846760129953 1101 38-39 0.2044360721388685 1101 40-41 0.1561728198217267 1101 42-43 0.06715662840205283 1101 44-45 0.16638809208502892 1101 46-47 0.2244329270035621 1101 48-49 0.5439467488368805 1101 50-51 0.3713300412339038 1101 52-53 0.3559324601372964 1101 54-55 0.3882430862854207 1101 56-57 0.45228645773888587 1101 58-59 0.6178113202585394 1101 60-61 0.5273226354625393 1101 62-63 0.25004105529977494 1101 64-65 0.09909544212959531 1101 66-67 0.2880755863552018 1101 68-69 0.13646011339143982 1101 70-71 0.22633240840664826 1101 72-73 0.29843600675344106 1101 74-75 0.3014374248473892 1101 76-77 0.34752484572385 1101 78-79 0.41243901149647755 1101 80-81 0.4018984813169375 1101 82-83 0.16902214983038988 1101 84-85 0.2901922715931846 1101 86-87 0.2078970471230761 1101 88-89 0.277292301876102 1101 90-91 0.12321270388972039 1101 92-93 0.19796601742639908 1101 94-95 0.04744103696465274 1101 96-97 0.3775323412312126 1101 98-99 0.4048117685804371 1101 100-101 0.09101114351231487 1102 1 0.39525153313513073 1102 2 0.44722368000281065 1102 3 0.2058604274927518 1102 4 0.15142769472173256 1102 5 0.18050688103993906 1102 6 0.09736365452923934 1102 7 0.03247893457740503 1102 8 -0.1357421547121973 1102 9 -0.1715829608353232 1102 10-11 0.17688567372979236 1102 12-13 0.08536379823722484 1102 14-15 0.1860712369022579 1102 16-17 0.2223136858214474 1102 18-19 0.15066872830325195 1102 20-21 0.037209830307133984 1102 22-23 0.07154627011851744 1102 24-25 0.07640138595471768 1102 26-27 -0.1293921276362795 1102 28-29 -0.06692167720181175 1102 30-31 0.08945874165979717 1102 32-33 0.15581678707240343 1102 34-35 0.13556200610954505 1102 36-37 0.10853400450375261 1102 38-39 -0.01043574447781026 1102 40-41 0.16024753959810312 1102 42-43 0.179992743178083 1102 44-45 0.005778191529827836 1102 46-47 -0.0019802595300433268 1102 48-49 0.1173202449741737 1102 50-51 0.19297441123835313 1102 52-53 0.09111780538899694 1102 54-55 -4.693066142138491E-4 1102 56-57 0.04544698446525075 1102 58-59 0.020052022009494408 1102 60-61 0.010143360295685966 1102 62-63 -0.04958906419381037 1102 64-65 -0.21688520057312033 1102 66-67 -0.08047032769922424 1102 68-69 -0.2621625111836501 1102 70-71 -0.4047348903838497 1102 72-73 -0.1456445732696423 1102 74-75 -0.5112562065648412 1102 76-77 -0.08740548638035861 1102 78-79 0.12625620115483116 1102 80-81 0.11623564555551624 1102 82-83 0.2479797326253994 1102 84-85 0.2569693479171917 1102 86-87 0.04434234541670179 1102 88-89 0.14164063754972744 1102 90-91 -0.08339877425694198 1102 92-93 0.06989652294606685 1102 94-95 -0.3652831460120396 1102 96-97 -0.022331372697419027 1102 98-99 -0.32080473970192 1102 100-101 -0.7745315300105133 1103 1 0.38036124160773 1103 2 0.3435204156606986 1103 3 0.20492150242313656 1103 4 0.06898464702648255 1103 5 -0.10302261906448962 1103 6 -0.08612867979118732 1103 7 -0.11526736130640103 1103 8 0.02112963469095064 1103 9 -0.04554362820408642 1103 10-11 -0.22796644872673255 1103 12-13 -0.05033295925319692 1103 14-15 -0.01702084274736393 1103 16-17 -0.03671731574279136 1103 18-19 -0.14063056605164803 1103 20-21 -0.02601459486943014 1103 22-23 0.07967194725827653 1103 24-25 -0.0217531972048306 1103 26-27 -0.13648706949296496 1103 28-29 -0.006410859056074969 1103 30-31 -0.1673943702016345 1103 32-33 -0.20392812566372243 1103 34-35 -0.30530748393677953 1103 36-37 -0.3283920633499946 1103 38-39 -0.23475933036390728 1103 40-41 -0.049682034711693746 1103 42-43 -0.12890761024391395 1103 44-45 -0.16063302610075425 1103 46-47 -0.48762947131885426 1103 48-49 -0.2284524783691566 1103 50-51 -0.6138332372149762 1103 52-53 -0.5602583066661495 1103 54-55 -0.37724043921151207 1103 56-57 -0.30471780067045273 1103 58-59 -0.44333242426765196 1103 60-61 -0.5249947115383904 1103 62-63 -0.5485898788122725 1103 64-65 -0.5802204921036775 1103 66-67 -0.3855588220180337 1103 68-69 -0.7459206218745855 1103 70-71 -0.8085938219401214 1103 72-73 -0.684401734308544 1103 74-75 -0.7047946605313236 1103 76-77 -0.6901938035780191 1103 78-79 -0.5295570890518917 1103 80-81 -0.44207614445338805 1103 82-83 -0.21904336379551737 1103 84-85 -0.19024955981259595 1103 86-87 -0.4948123443815149 1103 88-89 -0.6180103195796356 1103 90-91 -0.7039648299702819 1103 92-93 -0.3232654144955269 1103 94-95 -0.5794224236833543 1103 96-97 -0.5567627267371655 1103 98-99 -0.6388397361146971 1103 100-101 -0.5342313894872532 1104 1 0.2755627301920818 1104 2 0.4160481568426029 1104 3 0.1771056196627363 1104 4 -0.0860535862303351 1104 5 -0.006359710077298075 1104 6 0.050465232768061696 1104 7 0.005177584161970117 1104 8 0.056130381699517784 1104 9 -0.06143453778888386 1104 10-11 -0.10927357790488657 1104 12-13 -0.14508080990580652 1104 14-15 0.002618691662569006 1104 16-17 -0.08192491621544207 1104 18-19 0.06605312335577196 1104 20-21 -0.2216289754635099 1104 22-23 -0.08808580465338878 1104 24-25 0.09219956468958657 1104 26-27 -0.0686722732904883 1104 28-29 -0.04858967932581493 1104 30-31 -0.14293323502025146 1104 32-33 -0.04546723495786864 1104 34-35 0.04759294661180036 1104 36-37 -0.09740343146224006 1104 38-39 0.0068368232210573865 1104 40-41 0.001678199984816331 1104 42-43 -0.14164071087276398 1104 44-45 0.19307553190105864 1104 46-47 0.15125300386008433 1104 48-49 0.2964840259023731 1104 50-51 0.24592609020567835 1104 52-53 -0.03535575732963281 1104 54-55 -0.015939557741397437 1104 56-57 -0.09732784861481747 1104 58-59 -0.09561351680164165 1104 60-61 -0.2792492648951779 1104 62-63 -0.3075641681354817 1104 64-65 -0.2724665431447342 1104 66-67 0.08754207846684281 1104 68-69 -0.05180704940796588 1104 70-71 -0.09094268852439313 1104 72-73 0.05568682016196291 1104 74-75 0.014527055686908596 1104 76-77 0.14130143594009326 1104 78-79 0.13116520032564694 1104 80-81 0.08207939631290628 1104 82-83 0.07261594596856469 1104 84-85 0.20798070897467014 1104 86-87 0.37687195621473535 1104 88-89 0.32160422399812916 1104 90-91 0.05505020527493798 1104 92-93 0.2691667095893884 1104 94-95 0.2803233217632872 1104 96-97 0.17163174811581072 1104 98-99 0.5445576588626899 1104 100-101 0.3603792832279211 1105 1 0.3900661273039603 1105 2 0.4571411703045989 1105 3 0.15717267125111434 1105 4 0.08370304663198169 1105 5 -0.2985487168275398 1105 6 -0.1510303981029324 1105 7 0.10096085513153952 1105 8 0.09878478013197167 1105 9 -0.028896154657999773 1105 10-11 0.06761264661127342 1105 12-13 0.0010208406540357373 1105 14-15 0.04019858894103123 1105 16-17 0.2727904114169988 1105 18-19 0.10149986088585905 1105 20-21 -0.07669671428119074 1105 22-23 0.0756248597159157 1105 24-25 -0.13916030265866652 1105 26-27 0.03503282727040613 1105 28-29 0.1652370443277391 1105 30-31 0.06367978011309106 1105 32-33 0.2832400372476087 1105 34-35 0.05387862068478455 1105 36-37 0.2905737943085356 1105 38-39 -0.11250686729923842 1105 40-41 0.05807828399172621 1105 42-43 0.13762673568003692 1105 44-45 -0.029184869726734064 1105 46-47 0.337019880287464 1105 48-49 -0.045150304334946156 1105 50-51 -0.04388730229901938 1105 52-53 -0.07305660696496119 1105 54-55 -0.2921859442897343 1105 56-57 -0.2831810410423259 1105 58-59 -0.17924437460675335 1105 60-61 0.0648272033839703 1105 62-63 -0.01692170220281497 1105 64-65 -0.06609158424104677 1105 66-67 0.013326811860359555 1105 68-69 -0.23483600430263252 1105 70-71 -0.10319846097562291 1105 72-73 -0.18654336536795313 1105 74-75 -0.13943759763614594 1105 76-77 -0.26708704983535725 1105 78-79 -0.1791595127698642 1105 80-81 -0.3253931502510028 1105 82-83 0.04102593927220255 1105 84-85 0.19729071976141555 1105 86-87 -0.16578903961900338 1105 88-89 -0.26580364604186 1105 90-91 -0.540791342739297 1105 92-93 -0.4400995604268516 1105 94-95 -0.06903546581028408 1105 96-97 -0.05137086115639633 1105 98-99 0.05795698086975065 1105 100-101 0.5823070658743354 1106 1 0.309099361735953 1106 2 0.3464353490553371 1106 3 0.06960916644236192 1106 4 -0.0638161615205135 1106 5 -0.14017175347675703 1106 6 -0.04240359444696651 1106 7 -0.07055436512791147 1106 8 -0.0802868407555124 1106 9 -0.018057801633943882 1106 10-11 -0.2225811960341062 1106 12-13 -0.10637168622542958 1106 14-15 -0.148032350661488 1106 16-17 -0.12089089742381987 1106 18-19 -0.14031391406086158 1106 20-21 -0.11533539803769344 1106 22-23 -0.14578155912030866 1106 24-25 -0.14522198963294386 1106 26-27 -0.23123216464038165 1106 28-29 0.013453089030349474 1106 30-31 -0.30121616453399724 1106 32-33 -0.12116321157184728 1106 34-35 -0.28236105885100216 1106 36-37 -0.10697153215543409 1106 38-39 0.08994885096924321 1106 40-41 -0.13864931545187886 1106 42-43 -0.374649616950002 1106 44-45 -0.1718893537184485 1106 46-47 -0.2578030226359971 1106 48-49 -0.15740531457332452 1106 50-51 0.023225564625938944 1106 52-53 0.15162579436752566 1106 54-55 -0.28435567450996757 1106 56-57 -0.30414359783099343 1106 58-59 -0.34120402405423533 1106 60-61 0.15415332109844826 1106 62-63 -0.4702326086126938 1106 64-65 -0.47622145941043925 1106 66-67 -0.48488669563484876 1106 68-69 -0.46044150138633455 1106 70-71 -0.26584688760853936 1106 72-73 -0.12470415864823536 1106 74-75 -0.2648853401185498 1106 76-77 -0.17013737012048225 1106 78-79 -0.08323068591487015 1106 80-81 -0.20299901149859068 1106 82-83 0.23174707331436295 1106 84-85 -0.02746929026801226 1106 86-87 0.09846931973314099 1106 88-89 0.4578093969951489 1106 90-91 0.28427566938556836 1106 92-93 0.5964757767232385 1106 94-95 0.2550629300776457 1106 96-97 0.2588699049011396 1106 98-99 -0.23510465237542633 1106 100-101 -0.12451787278494564 1107 1 0.3292177513678922 1107 2 0.2696614665256547 1107 3 0.1529583822231615 1107 4 0.2984437799573385 1107 5 -0.1465144555855673 1107 6 0.13631037575382265 1107 7 0.0980723824084393 1107 8 0.18196693459567825 1107 9 -0.06537441542140243 1107 10-11 0.11039524936203549 1107 12-13 0.13429223744913088 1107 14-15 0.19340850497266615 1107 16-17 0.08166776486302751 1107 18-19 0.25702568256234315 1107 20-21 0.3648965604362502 1107 22-23 0.02797848886336851 1107 24-25 0.34869736864143874 1107 26-27 -0.18970890713172395 1107 28-29 0.20763142462459427 1107 30-31 0.3078668203197452 1107 32-33 0.29329719628940865 1107 34-35 0.5968052808241069 1107 36-37 0.47688638907510494 1107 38-39 0.4885188701402967 1107 40-41 0.5994276378088799 1107 42-43 0.33625013304159523 1107 44-45 0.37159865596797204 1107 46-47 0.6109425629778684 1107 48-49 0.8298139865242504 1107 50-51 0.9901737243174153 1107 52-53 0.5618567998946133 1107 54-55 0.43035586287357575 1107 56-57 0.577911011628963 1107 58-59 0.7958984009152061 1107 60-61 0.7721477603733149 1107 62-63 0.3538954450213936 1107 64-65 0.36069430008819836 1107 66-67 0.15459949784699134 1107 68-69 0.4455250669960087 1107 70-71 0.16994411433098833 1107 72-73 0.14604485757428165 1107 74-75 0.8928007367674446 1107 76-77 0.8157618193141403 1107 78-79 0.8835310885842738 1107 80-81 1.0253817075838896 1107 82-83 1.019129216211546 1107 84-85 0.9975441630992528 1107 86-87 1.3866979036541274 1107 88-89 1.5346085045884514 1107 90-91 1.3505503198782982 1107 92-93 1.160492784021688 1107 94-95 1.06028900270341 1107 96-97 1.263685089268325 1107 98-99 0.9711929248973519 1107 100-101 1.0099975817707012 1108 1 0.19273834360684816 1108 2 0.25779250485502914 1108 3 -0.10544018554459456 1108 4 -0.024444243039503988 1108 5 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-1.4297639765358312 2316 80-81 -1.0936933043919836 2316 82-83 -0.9054084330774756 2316 84-85 -1.3457335830612998 2316 86-87 -1.5355878714614377 2316 88-89 -1.3673960728575985 2316 90-91 -1.3344964167465925 2316 92-93 -1.5479775383238632 2316 94-95 -1.8960062139064142 2316 96-97 -2.2964092437879593 2316 98-99 -2.0703070689611707 2316 100-101 -2.0184906736819066 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 6.0 4 9.0 5 28.0 6 67.0 7 181.0 8 404.0 9 719.0 10 1174.0 11 1783.0 12 2551.0 13 3491.0 14 4442.0 15 5791.0 16 7387.0 17 9120.0 18 7932.0 19 5883.0 20 5700.0 21 5801.0 22 6380.0 23 6961.0 24 8278.0 25 10037.0 26 12113.0 27 14854.0 28 17857.0 29 21808.0 30 26108.0 31 32051.0 32 38956.0 33 48268.0 34 61087.0 35 78805.0 36 102296.0 37 109921.0 38 50521.0 39 1888.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.778669561806886 19.543901731968376 14.941850881331462 21.73557782489328 2 15.968550653459104 22.103621853552546 38.216275473084124 23.71155201990422 3 21.37282659476863 24.766124992260455 29.68059597318458 24.180452439786333 4 13.356234264991269 17.27955507833554 37.16382140650749 32.200389250165706 5 13.306991771076065 38.60488219467991 33.57226481150023 14.515861222743801 6 31.459452005448423 37.192400346717996 16.40029606114845 14.947851586685129 7 28.88326973084327 33.226531806873574 19.902232278545135 17.987966183738024 8 26.577538752490625 33.88197516688618 18.817049970168746 20.72343611045445 9 25.87875879673474 17.96398422251711 20.362831447456 35.79442553329215 10-11 25.701054580837713 26.49278172519547 26.599442913398292 21.206720780568528 12-13 27.685203816717628 23.77751479956041 26.93273745783867 21.604543925883295 14-15 22.914994227225684 25.860551442419656 26.87495558727865 24.349498743076012 16-17 21.52845789290016 28.884184368421646 27.400290432609502 22.18706730606869 18-19 21.365441333738588 28.094992851755542 29.601612013553524 20.93795380095235 20-21 22.658035302196254 25.85975932367476 29.569599698310313 21.91260567581867 22-23 21.794269246626712 26.25675959006947 29.737271285585027 22.21169987771879 24-25 22.90963099298684 26.299981425821485 29.737752299258158 21.052635281933515 26-27 22.386333334974992 25.957643807512277 30.687941620753 20.968081236759733 28-29 21.19693694910675 27.739128477029933 30.052809766640777 21.011124807222544 30-31 21.837675751128522 26.240952123647745 29.879169340222667 22.04220278500107 32-33 21.8710290237467 26.758135444151275 29.777238346525948 21.593597185576076 34-35 22.86147886367682 26.034231535311164 29.728400551318106 21.37588904969391 36-37 22.82257054526675 25.770323495763698 30.2445109569784 21.162595001991154 38-39 21.988340465171255 26.547181997655713 30.434819316609985 21.029658220563054 40-41 21.836662323078528 26.805731847694847 29.76734597383152 21.590259855395104 42-43 21.693071431838412 27.070847802300257 28.878122922010068 22.35795784385126 44-45 22.467381882450947 26.462186624038907 28.72906136371312 22.34137012979703 46-47 23.017589530711323 27.352353479209174 28.237669738971366 21.39238725110814 48-49 23.127845832656067 27.291917782482866 28.05087685597017 21.529359528890897 50-51 21.888792186986613 27.658565517571333 28.886155907075178 21.56648638836688 52-53 22.741104679510947 28.039634667599163 27.58124536949414 21.638015283395752 54-55 21.721337141514166 27.414680072701636 28.17188321297315 22.692099572811053 56-57 22.909234953991984 27.249495874827794 27.99382807256025 21.84744109861997 58-59 23.752336741205514 26.99784777076472 27.445671866304277 21.804143621725483 60-61 22.3251395822559 27.19959471012275 29.101994434300472 21.373271273320878 62-63 22.089983852299554 27.19884890466524 28.849854529975268 21.861312713059935 64-65 21.80672928264662 28.367262637372693 28.052001241341745 21.774006838638943 66-67 22.246136267373252 27.463142741755046 27.574168328298015 22.716552662573683 68-69 21.86645165647901 27.631764096675788 27.874283768419396 22.62750047842581 70-71 22.793977966867278 27.54240090507798 27.605159732472913 22.058461395581837 72-73 22.545957020476624 26.904275438181756 28.560332308939245 21.989435232402375 74-75 21.898393614985423 28.71441356252744 27.489221347922506 21.89797147456463 76-77 22.41348498642805 27.359049442980226 27.410621439034582 22.816844131557136 78-79 22.36736279255336 27.72154401608274 27.62830972922652 22.282783462137377 80-81 22.577635227897243 27.82406035883445 27.61084539381789 21.987459019450412 82-83 22.42801452375591 27.45566876829543 28.04309277189822 22.07322393605044 84-85 21.842454673580118 27.99307060064411 27.79865619751321 22.36581852826256 86-87 21.92553782110416 28.14563688165438 27.280633796127223 22.648191501114237 88-89 21.782155919816848 28.040808024789975 26.90314639273642 23.273889662656757 90-91 22.316094530391755 27.347900470174764 27.268024949312476 23.067980050121008 92-93 22.566432700480128 27.88688851239158 27.386708394076358 22.159970393051935 94-95 22.13218743029555 27.778907512706 27.331532481130104 22.757372575868345 96-97 21.394414124839216 27.917835902603233 27.957733000458777 22.73001697209877 98-99 21.912247432546064 28.321611646864696 26.62728555294813 23.13885536764111 100-101 21.366543455116446 28.04642492996785 26.92605125628636 23.660980358629345 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 187.0 1 147.5 2 212.0 3 291.0 4 230.0 5 197.5 6 340.5 7 667.5 8 963.5 9 1107.5 10 1026.5 11 923.5 12 939.5 13 999.5 14 1108.5 15 1210.5 16 1305.0 17 1377.0 18 1443.0 19 1539.0 20 1669.0 21 1858.0 22 2127.5 23 2445.5 24 2957.5 25 3634.5 26 4396.5 27 5307.0 28 6183.5 29 7115.5 30 8454.0 31 9845.0 32 11273.0 33 12400.5 34 13709.5 35 15008.5 36 16145.0 37 17311.5 38 18053.0 39 18248.5 40 18654.5 41 20227.5 42 21521.0 43 22432.5 44 23357.0 45 24063.0 46 24635.5 47 29071.5 48 40077.0 49 39300.0 50 29100.5 51 25114.0 52 23145.5 53 22328.5 54 21480.0 55 19771.5 56 17556.0 57 15523.0 58 14131.5 59 12523.0 60 10648.5 61 8959.5 62 7515.5 63 6032.0 64 4536.0 65 3457.5 66 2604.5 67 2116.0 68 1580.0 69 1062.5 70 793.0 71 642.5 72 551.5 73 459.0 74 368.5 75 304.5 76 226.0 77 154.0 78 107.5 79 78.5 80 61.0 81 42.5 82 28.5 83 19.0 84 14.0 85 9.0 86 5.0 87 4.5 88 2.5 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.9788029223374196 2 0.008583521889387952 3 0.003939977260702667 4 0.009427802730967095 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004362117681492238 10-11 3.5178368399130954E-4 12-13 1.4071347359652383E-4 14-15 7.035673679826192E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.849943151756667E-4 24-25 0.0 26-27 7.035673679826192E-5 28-29 0.0 30-31 0.0 32-33 0.005487825470264429 34-35 4.924971575878334E-4 36-37 0.002954982945527 38-39 1.4071347359652383E-4 40-41 0.005347111996667905 42-43 0.012875282834081929 44-45 0.0022514155775443813 46-47 0.0019699886303513334 48-49 0.012031001992502786 50-51 4.924971575878334E-4 52-53 0.0033067666295183095 54-55 0.012453142413292357 56-57 0.003799263787106143 58-59 7.739241047808811E-4 60-61 0.008653878626186214 62-63 0.00513604178627312 64-65 0.02005166998750464 66-67 0.0023921290511409047 68-69 0.0 70-71 9.849943151756667E-4 72-73 0.0 74-75 0.0 76-77 9.849943151756667E-4 78-79 0.012453142413292357 80-81 0.015970979253205453 82-83 0.014634201254038475 84-85 0.009638872941361882 86-87 0.012453142413292357 88-89 0.03032375356005088 90-91 0.025820922404962122 92-93 0.0028142694719304764 94-95 0.010483153782941023 96-97 0.012101358729301048 98-99 0.01217171546609931 100-101 0.013719563675661071 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 710664.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.83311916573835 #Duplication Level Percentage of deduplicated Percentage of total 1 86.09211271971436 55.8162020318742 2 8.486231174841766 11.0037767405304 3 2.184189336494321 4.248234226004138 4 0.9276971876275377 2.405820092607059 5 0.5598293524183676 1.814774155890908 6 0.33774535420724794 1.3138250878195807 7 0.2382245735739574 1.081138951670931 8 0.17528772755566954 0.9091560103126561 9 0.13711672106101197 0.8000734244547505 >10 0.7751300160201026 9.288274467025847 >50 0.052688985972767076 2.3924608703851216 >100 0.028521531724526858 3.44631677882734 >500 0.001959494545623214 0.860767549263737 >1k 0.003048102626525 3.4200618155314997 >5k 2.177216161803571E-4 1.1991177978018115 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8495 1.1953609582024698 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3401 0.4785665237017775 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2925 0.41158691026983213 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2165 0.3046446703364741 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2054 0.2890254747672599 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1800 0.25328425247374287 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1538 0.21641732239145364 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1450 0.20403453671495952 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1379 0.19404388008960635 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1355 0.19066675672328978 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1329 0.18700820640978014 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1325 0.18644535251539407 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1190 0.16744903357986332 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1177 0.16561975842310853 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1141 0.16055407337363367 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 959 0.13494422117906632 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 845 0.11890288518906261 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.03567367982619E-5 0.0 12-13 0.0 0.0 0.0 2.814269471930476E-4 0.0 14-15 0.0 0.0 0.0 2.814269471930476E-4 0.0 16-17 0.0 0.0 0.0 4.221404207895714E-4 0.0 18-19 0.0 0.0 0.0 4.221404207895714E-4 0.0 20-21 0.0 0.0 0.0 4.221404207895714E-4 0.0 22-23 0.0 0.0 0.0 4.924971575878334E-4 0.0 24-25 0.0 0.0 0.0 5.628538943860952E-4 0.0 26-27 0.0 0.0 0.0 0.0010553510519739286 0.0 28-29 0.0 0.0 0.0 0.0020403453671495953 0.0 30-31 0.0 0.0 0.0 0.0047139013654835474 0.0 32-33 0.0 0.0 0.0 0.009076019046975786 0.0 34-35 0.0 0.0 0.0 0.012804926097283668 0.0 36-37 0.0 0.0 0.0 0.019770243040311594 0.0 38-39 0.0 0.0 0.0 0.03257516913759526 0.0 40-41 0.0 0.0 0.0 0.048194364706809406 0.0 42-43 0.0 0.0 0.0 0.07063816374545495 0.0 44-45 0.0 0.0 0.0 0.10096191730550583 0.0 46-47 0.0 0.0 0.0 0.13846205801897943 0.0 48-49 0.0 0.0 0.0 0.18187216462350703 0.0 50-51 0.0 0.0 0.0 0.23266972859185212 0.0 52-53 0.0 0.0 0.0 0.28916618824085644 7.03567367982619E-5 54-55 0.0 0.0 0.0 0.34903977125617736 1.407134735965238E-4 56-57 0.0 0.0 0.0 0.4329753582565038 1.407134735965238E-4 58-59 0.0 0.0 0.0 0.5284494500917452 1.407134735965238E-4 60-61 0.0 0.0 0.0 0.6377134623394459 1.407134735965238E-4 62-63 0.0 0.0 0.0 0.7616820325779834 1.407134735965238E-4 64-65 0.0 0.0 0.0 0.8781927887159051 1.407134735965238E-4 66-67 0.0 0.0 0.0 1.027489784201817 1.407134735965238E-4 68-69 0.0 0.0 0.0 1.2089398084045344 1.407134735965238E-4 70-71 0.0 0.0 0.0 1.4038983260725182 1.407134735965238E-4 72-73 0.0 0.0 0.0 1.6325577206668693 1.407134735965238E-4 74-75 0.0 0.0 0.0 1.8608653315772292 1.407134735965238E-4 76-77 0.0 0.0 0.0 2.100430020375311 1.407134735965238E-4 78-79 0.0 0.0 0.0 2.3812237569371746 1.407134735965238E-4 80-81 0.0 0.0 0.0 2.73842490966195 1.407134735965238E-4 82-83 0.0 0.0 0.0 3.0810622178694853 1.407134735965238E-4 84-85 0.0 0.0 0.0 3.447972600272421 1.407134735965238E-4 86-87 0.0 0.0 0.0 3.882214379791294 1.407134735965238E-4 88-89 0.0 0.0 0.0 4.356700212758772 1.407134735965238E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACACG 130 7.55972E-9 33.191624 1 TACCTGG 520 0.0 26.487463 2 GGTAAGG 190 1.1908924E-8 24.997242 3 GTACATG 3395 0.0 23.442162 1 TACACGG 165 2.4300425E-6 23.02776 2 TAAGGTG 310 0.0 22.979717 5 TACATGG 3495 0.0 22.422417 2 GTAAGGT 225 7.1502654E-8 21.108782 4 GAGTACT 3735 0.0 20.915094 12-13 GTACCTG 665 0.0 20.907675 1 CGTGAAC 115 0.0037242807 20.649895 3 ACATGGG 3485 0.0 20.442507 3 CTGGTCG 140 4.855144E-4 20.353464 9 ATTCGCG 95 8.251336E-6 20.000608 80-81 GTGTAAG 265 1.6641934E-8 19.901058 1 GGACGCT 145 6.150353E-4 19.65162 6 GTACTTT 3945 0.0 19.621181 14-15 CTGGACG 195 1.0327209E-5 19.48503 4 AGTACTT 3825 0.0 19.367687 12-13 GCGACAG 175 9.9437646E-5 18.996565 7 >>END_MODULE