FastQCFastQC Report
Thu 26 May 2016
SRR936397_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936397_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences774908
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT52880.6824035885550285No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT46290.5973612351401715No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40010.5163193566203989No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30160.38920749301852603No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC29100.37552844982888295No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT28380.36623702426610644No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19770.255127060244571No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT17480.2255751650518513No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT14000.1806666081650983No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC13350.1722785156431473No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC11400.14711423807729432No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC10210.131757576383261No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA9860.12724091117913353No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG9310.12014329442979037No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA8570.11059377371249232No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA8210.1059480609311041No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG600.00394109231.6815412
TCCCGTA600.00394109231.6815412
CGTCATA404.4854032E-429.69760914-15
TACCTGG6600.029.5214352
GTACCTG6950.028.7185211
GTACATG35650.027.59361
CCAACGC700.00837071327.1556073
TACATGG37900.025.3285142
ACATGGG35550.024.9976713
GAGTACT31650.023.87222512-13
GGACCGT1000.001648647623.7611566
ACCGTCC1252.2478214E-422.8107098
TATCGGC1050.002189595722.6296712
AGTACTT32950.022.20929712-13
GTACTTT34750.021.80958214-15
GTCTAGG2751.2496457E-920.737011
CATGGGG14950.020.3453144
CATGGGT6100.020.256724
CATGGGA20500.019.9374774
GTATCGG1200.004749201219.8009641
Produced by FastQC (version 0.11.5)