##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936397_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 774908 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.787505097379302 33.0 31.0 34.0 30.0 34.0 2 31.85289737620466 33.0 31.0 34.0 30.0 34.0 3 31.903098174234877 33.0 31.0 34.0 30.0 34.0 4 35.52309822585391 37.0 35.0 37.0 33.0 37.0 5 35.500867199719195 37.0 35.0 37.0 33.0 37.0 6 35.624949026206984 37.0 35.0 37.0 33.0 37.0 7 35.522238768989354 37.0 35.0 37.0 33.0 37.0 8 35.49040789358221 37.0 35.0 37.0 33.0 37.0 9 37.06104724689899 39.0 37.0 39.0 33.0 39.0 10-11 36.958819627620315 39.0 37.0 39.0 33.0 39.0 12-13 36.870324606275844 39.0 37.0 39.0 33.0 39.0 14-15 37.99375732345001 40.0 38.0 41.0 33.0 41.0 16-17 37.90080693450061 40.0 38.0 41.0 33.0 41.0 18-19 37.882522183278525 40.0 38.0 41.0 33.0 41.0 20-21 37.794378816582096 40.0 37.5 41.0 32.5 41.0 22-23 37.65894725567422 40.0 37.0 41.0 32.0 41.0 24-25 37.78208574437224 40.0 37.0 41.0 33.0 41.0 26-27 37.74915796455838 40.0 37.0 41.0 33.0 41.0 28-29 37.66108170260212 40.0 37.0 41.0 33.0 41.0 30-31 37.47628557196467 40.0 37.0 41.0 32.0 41.0 32-33 37.27285303545711 39.0 36.5 41.0 32.0 41.0 34-35 37.02632506052332 39.0 36.0 41.0 31.0 41.0 36-37 36.834315170317 39.0 36.0 41.0 31.0 41.0 38-39 36.63765956732928 39.0 35.0 40.5 30.0 41.0 40-41 36.476066836321216 39.0 35.0 40.0 30.0 41.0 42-43 36.238263768086014 39.0 35.0 40.0 30.0 41.0 44-45 35.94932817831278 38.5 35.0 40.0 29.5 41.0 46-47 35.63375910430658 38.5 35.0 40.0 28.0 41.0 48-49 35.51159815100631 39.0 35.0 40.0 27.0 41.0 50-51 34.57615226581736 38.0 34.0 39.5 25.5 40.5 52-53 34.7272876264021 38.0 34.5 39.5 26.5 40.5 54-55 35.15578623526922 38.5 35.0 40.0 27.0 41.0 56-57 35.037604464013796 38.0 35.0 40.0 26.0 41.0 58-59 34.77758262916372 38.0 34.0 40.0 26.0 41.0 60-61 34.433934092821346 38.0 34.0 40.0 25.5 41.0 62-63 34.14120192332509 37.5 34.0 40.0 24.5 41.0 64-65 33.81276035348712 37.0 33.5 40.0 23.5 41.0 66-67 33.408198134488224 36.0 33.0 39.0 22.5 41.0 68-69 33.00340233937448 36.0 33.0 39.0 22.0 41.0 70-71 32.52862339787433 35.0 32.0 38.5 20.5 40.0 72-73 32.12912887723446 35.0 32.0 38.0 19.5 40.0 74-75 31.710906972182503 35.0 31.0 37.0 18.0 39.0 76-77 31.271304464529983 35.0 31.0 36.5 15.5 39.0 78-79 30.804481951405844 35.0 31.0 36.0 11.5 39.0 80-81 30.313877260268313 34.0 30.0 36.0 7.0 37.5 82-83 29.831452895053346 34.0 30.0 35.0 6.0 37.0 84-85 29.499502521589662 34.0 29.0 35.0 2.0 36.5 86-87 29.21391571644634 34.0 29.0 35.0 2.0 36.0 88-89 28.805645315314848 34.0 29.0 35.0 2.0 36.0 90-91 28.368994770992167 34.0 29.0 35.0 2.0 35.5 92-93 28.014255240622113 34.0 28.0 35.0 2.0 35.0 94-95 27.569617296504877 34.0 27.0 35.0 2.0 35.0 96-97 26.98118099180806 33.5 25.5 35.0 2.0 35.0 98-99 26.31333009854073 33.0 25.0 35.0 2.0 35.0 100-101 23.84912337980767 31.0 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.36463316008613234 1101 2 0.3246446674543044 1101 3 0.4842074493324482 1101 4 0.4743943209537065 1101 5 0.4496828138317923 1101 6 0.2276656355493003 1101 7 0.2879998134329611 1101 8 0.41952996544953436 1101 9 0.5262793662644185 1101 10-11 0.5850006567360708 1101 12-13 0.5252833956629814 1101 14-15 0.8251855360102667 1101 16-17 0.6733227924815779 1101 18-19 0.6960027850430635 1101 20-21 0.6280197320775756 1101 22-23 0.6049176465533179 1101 24-25 0.6137479280523195 1101 26-27 0.6254543435748303 1101 28-29 0.6157506768177257 1101 30-31 0.5891099906437631 1101 32-33 0.564421319988611 1101 34-35 0.4265131700438545 1101 36-37 0.36917681302897165 1101 38-39 0.4065089762067515 1101 40-41 0.43748159509629403 1101 42-43 0.4202470214032985 1101 44-45 0.36037888860121114 1101 46-47 0.5839838788165963 1101 48-49 0.6555162839526858 1101 50-51 0.5010242277024375 1101 52-53 0.38524401251044793 1101 54-55 0.5274842864441709 1101 56-57 0.4100014347393284 1101 58-59 0.575449988369499 1101 60-61 0.556252394527867 1101 62-63 0.5154124184675197 1101 64-65 0.4198367411082131 1101 66-67 0.40770309291894336 1101 68-69 0.7179978277055952 1101 70-71 0.503407045947057 1101 72-73 0.44659891360658577 1101 74-75 0.3320840545933592 1101 76-77 0.313603312312992 1101 78-79 0.16102140639041806 1101 80-81 0.37579339426311975 1101 82-83 0.3802142173839549 1101 84-85 0.39407824434359284 1101 86-87 0.372866962316067 1101 88-89 0.22955729290263704 1101 90-91 0.18187670729087557 1101 92-93 0.28725490966163036 1101 94-95 0.3618522217717768 1101 96-97 0.24711188671331286 1101 98-99 0.274148161804316 1101 100-101 0.8091597203020875 1102 1 0.3015030832480363 1102 2 0.38146470371230734 1102 3 0.23533519600509223 1102 4 0.31645341181334175 1102 5 0.44011637691785666 1102 6 0.2505286532718074 1102 7 0.35191419468798557 1102 8 0.3057054379855231 1102 9 0.4472906145546247 1102 10-11 0.36991753332533506 1102 12-13 0.2983264176779059 1102 14-15 0.38545433582669375 1102 16-17 0.3599543583112208 1102 18-19 0.4922839161425614 1102 20-21 0.5219146194611568 1102 22-23 0.3304632722017615 1102 24-25 0.2522498571642515 1102 26-27 0.17705079373193655 1102 28-29 0.242090695035742 1102 30-31 0.2187073942302078 1102 32-33 0.2232135406382696 1102 34-35 0.09463544834433435 1102 36-37 0.2624693783571743 1102 38-39 0.13832458653504887 1102 40-41 0.19247102726187393 1102 42-43 0.3881597727293524 1102 44-45 0.07609697220826206 1102 46-47 0.20289105495475468 1102 48-49 0.3305548810269201 1102 50-51 0.5484627765117835 1102 52-53 0.4456151774052586 1102 54-55 0.7837097645682078 1102 56-57 0.7014095924942936 1102 58-59 0.7361003577244531 1102 60-61 0.8348013720144749 1102 62-63 0.9163517046021425 1102 64-65 0.79307292157538 1102 66-67 0.7009100573047959 1102 68-69 0.5402060056368754 1102 70-71 0.6299384423550833 1102 72-73 0.4931352156260971 1102 74-75 0.35771414379438937 1102 76-77 0.5896680822091511 1102 78-79 0.3853146670099292 1102 80-81 0.723561439940692 1102 82-83 0.8335023617276178 1102 84-85 0.7713449879571499 1102 86-87 0.6899675184502065 1102 88-89 0.7539055932961745 1102 90-91 0.7726762626209336 1102 92-93 0.4979410326164313 1102 94-95 0.48044364862228406 1102 96-97 0.4514634904823289 1102 98-99 0.6529006826061838 1102 100-101 0.5647368862630735 1103 1 0.1936724646260508 1103 2 0.2860293636726503 1103 3 0.2902159667132018 1103 4 0.2622629979107032 1103 5 0.3867097515687874 1103 6 0.27776400784529187 1103 7 0.11292616433305369 1103 8 0.09746164341079577 1103 9 0.30470857628912995 1103 10-11 0.4277907152992384 1103 12-13 0.30988340585359 1103 14-15 0.44064135504249435 1103 16-17 0.5656625190553584 1103 18-19 0.5812101777306964 1103 20-21 0.6531083657628187 1103 22-23 0.5613097638396098 1103 24-25 0.5600970858747942 1103 26-27 0.5317280908552817 1103 28-29 0.06636893478161454 1103 30-31 0.2573959239259267 1103 32-33 0.47955651820426937 1103 34-35 0.5010194320664496 1103 36-37 0.44355048749584114 1103 38-39 0.5357443130914632 1103 40-41 0.4097984640137682 1103 42-43 0.3919697117910843 1103 44-45 0.40530114134048745 1103 46-47 0.5350149662153498 1103 48-49 0.5416405579399353 1103 50-51 0.5560099854978446 1103 52-53 0.5208536878955599 1103 54-55 0.5287734538765037 1103 56-57 0.5331026795830667 1103 58-59 0.33002521783636496 1103 60-61 0.5031368335154056 1103 62-63 0.5191681527423313 1103 64-65 0.7054002026076915 1103 66-67 0.5915456008180442 1103 68-69 0.6053071714574614 1103 70-71 0.6246413813026237 1103 72-73 0.5622253980256247 1103 74-75 0.3995845435784595 1103 76-77 0.30737443660615327 1103 78-79 0.4828522213820179 1103 80-81 0.30312116878099715 1103 82-83 0.201677951126225 1103 84-85 0.3073473548947696 1103 86-87 0.41299688716302896 1103 88-89 0.4697303360993317 1103 90-91 0.42243956885906186 1103 92-93 0.5643294802998398 1103 94-95 0.4263648337521033 1103 96-97 0.5587049700259641 1103 98-99 0.8517935163911048 1103 100-101 0.701725996273769 1104 1 0.010001121157838355 1104 2 0.1637373330437626 1104 3 0.2692235298934271 1104 4 0.29141831619001124 1104 5 0.29737741653183747 1104 6 0.06119445805526169 1104 7 0.24379486303924125 1104 8 0.1747977835484562 1104 9 0.22569848119778868 1104 10-11 0.381036806209309 1104 12-13 0.1659258117478828 1104 14-15 0.2188510045174894 1104 16-17 0.1594401666205627 1104 18-19 0.30341069785757924 1104 20-21 0.3572165724101666 1104 22-23 0.04804797508057135 1104 24-25 0.11583855604707338 1104 26-27 -0.2335738757162602 1104 28-29 0.11614703085678713 1104 30-31 0.2757794071845723 1104 32-33 0.05899703818647595 1104 34-35 -0.2207788758935223 1104 36-37 -0.08444449208610649 1104 38-39 0.34159158097850906 1104 40-41 0.180195720868916 1104 42-43 0.24665356651726 1104 44-45 0.12478455913114317 1104 46-47 0.10556811645534481 1104 48-49 -0.11032221573253054 1104 50-51 0.03838788408395999 1104 52-53 0.11555765323009126 1104 54-55 0.3689953939714883 1104 56-57 0.38039335383099626 1104 58-59 0.3128223285640743 1104 60-61 0.4184154703344589 1104 62-63 0.529531988370664 1104 64-65 0.07264269820397118 1104 66-67 0.3106503170450594 1104 68-69 0.38558611572286594 1104 70-71 0.5811529141577836 1104 72-73 0.5928861183181695 1104 74-75 0.4854684156663076 1104 76-77 0.5027066776006883 1104 78-79 0.44188228240107463 1104 80-81 0.33742241556637254 1104 82-83 0.46090730972079896 1104 84-85 0.5092665435257651 1104 86-87 0.37757762076618917 1104 88-89 0.505982601255532 1104 90-91 0.6648092929892577 1104 92-93 0.7557094080318656 1104 94-95 0.5636085229636301 1104 96-97 0.567261744515875 1104 98-99 0.5379819412168558 1104 100-101 1.0874948472563304 1105 1 0.24222383027998973 1105 2 0.2830328490236518 1105 3 0.2013068981030166 1105 4 0.2917166928437638 1105 5 0.321194372794551 1105 6 0.3200918430743869 1105 7 0.31149288223185323 1105 8 0.42759666488219494 1105 9 0.4698848139714542 1105 10-11 0.493860601501261 1105 12-13 0.4261407808023776 1105 14-15 0.2991906418372139 1105 16-17 0.3344572765029383 1105 18-19 0.30481149254391227 1105 20-21 0.46729096962532424 1105 22-23 0.22881449781086616 1105 24-25 0.38815107358777823 1105 26-27 0.46761224533800316 1105 28-29 0.2854619954799489 1105 30-31 0.3493002024617482 1105 32-33 0.31631735980302267 1105 34-35 0.2586779407328592 1105 36-37 0.30797102940833554 1105 38-39 0.29607141593584174 1105 40-41 0.035209778052852414 1105 42-43 0.3825887508362342 1105 44-45 0.077843412202796 1105 46-47 0.3165607850553869 1105 48-49 0.3560079976117052 1105 50-51 0.262884311934414 1105 52-53 0.33289698035626003 1105 54-55 0.12262785892699668 1105 56-57 0.42352896749723357 1105 58-59 0.24002974957549839 1105 60-61 0.055092657322084904 1105 62-63 0.2346575675217153 1105 64-65 0.47504694440902284 1105 66-67 0.6187025477305852 1105 68-69 0.596968249559076 1105 70-71 0.615666624220367 1105 72-73 0.7622655835051049 1105 74-75 0.8437045468141697 1105 76-77 0.39176176896007675 1105 78-79 0.5244159236302295 1105 80-81 0.5513343501401522 1105 82-83 0.5875864169542204 1105 84-85 0.7617951133365466 1105 86-87 0.8057338401468854 1105 88-89 0.5487509063678218 1105 90-91 0.561510901564386 1105 92-93 0.8203749159861147 1105 94-95 0.5929188593020669 1105 96-97 0.8143442018396421 1105 98-99 0.8663498800053269 1105 100-101 1.1187155819719123 1106 1 0.0509323229748162 1106 2 0.12576223455576496 1106 3 0.09111625553776648 1106 4 0.13714601517089164 1106 5 0.2652079879085676 1106 6 0.15304205190422238 1106 7 0.32908751726881036 1106 8 0.23051535568030346 1106 9 0.39719344996845507 1106 10-11 0.44920747581060994 1106 12-13 0.38590848488480844 1106 14-15 0.5679744174412207 1106 16-17 0.6906905690603722 1106 18-19 0.43376022049078955 1106 20-21 0.4781709547601878 1106 22-23 0.48534931615636623 1106 24-25 0.14234280288462742 1106 26-27 -0.062167297361959584 1106 28-29 0.25569793729757606 1106 30-31 0.25063496787835504 1106 32-33 0.2024090253318107 1106 34-35 0.04526398467791637 1106 36-37 -0.03126448588121633 1106 38-39 0.05282301752765761 1106 40-41 0.010111486558216143 1106 42-43 -0.03519127610682915 1106 44-45 0.19197064144544385 1106 46-47 -0.030700637239782225 1106 48-49 0.07719658918221484 1106 50-51 -0.024656141367799478 1106 52-53 0.20832544399650743 1106 54-55 0.17844672948628926 1106 56-57 0.2278736415974123 1106 58-59 0.20878973532974499 1106 60-61 0.16307253307994785 1106 62-63 0.07219119355935533 1106 64-65 -1.5178529745440983E-4 1106 66-67 0.15049507771775694 1106 68-69 -0.0906245695662733 1106 70-71 0.040808835437630364 1106 72-73 0.15176876979651865 1106 74-75 -0.10358756297950222 1106 76-77 -0.12096439297701167 1106 78-79 0.053260060699180656 1106 80-81 0.25462283937207886 1106 82-83 0.4243148480769712 1106 84-85 0.31026869182263894 1106 86-87 0.4366540287201559 1106 88-89 0.6407247178255169 1106 90-91 0.42195457522395685 1106 92-93 0.5821568742708223 1106 94-95 0.47257107703690693 1106 96-97 0.7131521755215964 1106 98-99 0.6986441083625472 1106 100-101 1.4578553747890695 1107 1 0.34706955602214506 1107 2 0.31977897496214425 1107 3 0.2564825715677408 1107 4 0.24078709987018243 1107 5 0.3771374571370316 1107 6 0.35383584727112805 1107 7 0.4381533334330925 1107 8 0.3836412915777032 1107 9 0.4417321316691698 1107 10-11 0.39860881022663364 1107 12-13 0.4063646933772773 1107 14-15 0.5087801614198284 1107 16-17 0.5893967536247473 1107 18-19 0.6135976329216675 1107 20-21 0.6863237981901449 1107 22-23 0.650819063321272 1107 24-25 0.5568600573591809 1107 26-27 0.17018534414572173 1107 28-29 0.5653144084742578 1107 30-31 0.5416840065862942 1107 32-33 0.5135159854753226 1107 34-35 0.6157414968966819 1107 36-37 0.48542343305346947 1107 38-39 0.6973610817290563 1107 40-41 0.3547656406304682 1107 42-43 0.3429807406768077 1107 44-45 0.4068912773462401 1107 46-47 0.5617868661178633 1107 48-49 0.29264595886979095 1107 50-51 0.10767318954081873 1107 52-53 0.26648152929785596 1107 54-55 0.10699202460464363 1107 56-57 0.33899495859994744 1107 58-59 0.5285957170455902 1107 60-61 0.5049687808453669 1107 62-63 0.6054890721404647 1107 64-65 0.6309332844954056 1107 66-67 0.4260441117944609 1107 68-69 0.6150621359822637 1107 70-71 0.6336475415295695 1107 72-73 0.49550979675962736 1107 74-75 0.5318825238100224 1107 76-77 0.7253894279543651 1107 78-79 0.7315506373509137 1107 80-81 0.592225923163646 1107 82-83 0.5669818893598872 1107 84-85 0.5050526751254587 1107 86-87 0.39318362083098535 1107 88-89 0.20218828382230214 1107 90-91 0.24505260015240182 1107 92-93 0.2815304212177523 1107 94-95 0.3726550569434792 1107 96-97 0.4118248607482293 1107 98-99 0.6253932523541259 1107 100-101 1.1671598544378377 1108 1 -0.08140159377428802 1108 2 0.05755332097739796 1108 3 0.023723278510818346 1108 4 0.09802967868373003 1108 5 0.03654282628548344 1108 6 -0.13812680891306428 1108 7 -0.3255113209728222 1108 8 0.011248261175403229 1108 9 0.13369244801528168 1108 10-11 -0.0681713385870708 1108 12-13 0.2686281747443644 1108 14-15 0.22225723880607262 1108 16-17 0.0739323737598312 1108 18-19 0.25445393566552355 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1804.0 12 2418.0 13 3160.0 14 3666.0 15 4498.0 16 5979.0 17 8155.0 18 6873.0 19 4343.0 20 4341.0 21 4497.0 22 4928.0 23 5681.0 24 6251.0 25 7168.0 26 8337.0 27 10091.0 28 12120.0 29 15109.0 30 18500.0 31 23704.0 32 30667.0 33 40398.0 34 55826.0 35 78843.0 36 112454.0 37 149132.0 38 119207.0 39 18811.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.693340768623074 17.743105575950967 14.224251436714841 22.33930221871111 2 15.215073610082117 19.908281037433625 40.41713670591333 24.459508646570928 3 20.502753060892594 24.150784615900893 29.590693544181917 25.755768779024592 4 12.211490418196066 15.698717046612124 38.10824733616426 33.98154519902755 5 13.168040449113239 37.5451902120583 34.29433547147594 14.992433867352526 6 31.22408192564514 35.65539483265251 17.642623645030184 15.477899596672156 7 28.38936494636123 32.748249590398345 20.662644458015347 18.199741005225086 8 26.127769558290794 33.129610551239246 19.718118933824954 21.024500956645003 9 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26.13327809868934 30.162051734729168 22.222716925030586 34-35 22.61457284065017 25.81179086669536 29.521304569749713 22.05233172290476 36-37 22.306118274289542 25.41278169725277 30.42036718808984 21.860732840367845 38-39 21.92648538283781 25.981140269647813 30.201832541649697 21.890541805864675 40-41 21.77613138347021 26.34347754267691 30.085418355073994 21.794972718778872 42-43 21.469328286605286 26.500161635532198 29.09788015483458 22.932629923027932 44-45 21.805204073336576 26.220781449825974 29.027168912353062 22.946845564484384 46-47 22.46422305687597 26.618213446671884 28.827966764979728 22.08959673147242 48-49 22.397104664270234 26.48097855017702 28.642091930393732 22.479824855159013 50-51 21.51622261280723 26.913651462241873 29.343042898106354 22.227083026844547 52-53 22.118586705502562 27.316006449993512 28.172069514876085 22.393337329627837 54-55 21.41746804747435 26.529484984716607 28.772017672858535 23.28102929495051 56-57 22.277046583139466 26.635475721075508 28.258996705590818 22.828480990194212 58-59 22.92637976238797 26.469136312335085 28.05566866214656 22.548815263130383 60-61 22.216973765892174 26.414985977587367 29.472362360308246 21.895677896212206 62-63 21.867315208490755 26.775257415309607 29.125855873237523 22.231571502962122 64-65 21.512051438397485 27.545158615705745 28.527275836148185 22.41551410974858 66-67 21.657135998823023 27.11639545883358 27.965379865189767 23.261088677153626 68-69 21.336744169837974 26.926420280193682 28.621405537403575 23.115430012564765 70-71 22.20729484263337 26.809079030150514 28.033812450596074 22.949813676620046 72-73 21.98508569242096 26.34458799822169 28.99682343444615 22.673502874911197 74-75 21.394280404707825 27.766750980797028 28.191526429898822 22.647442184596326 76-77 21.729793629100218 26.838412269460438 28.126826926427373 23.30496717501197 78-79 21.964014701783004 26.858758607873302 28.378816114145305 22.798410576198393 80-81 22.24376116382559 27.019878062630998 27.985258587756707 22.75110218578671 82-83 21.945914065711268 26.718134061723447 28.656356410420216 22.679595462145073 84-85 21.779567498499603 27.170929084467506 28.10265941313509 22.946844003897805 86-87 21.73337075860378 27.39942765146749 28.149546218595844 22.71765537133289 88-89 21.60472596927862 27.194004136191026 27.514058855223634 23.68721103930672 90-91 21.722474268579823 26.849594033652973 27.94428036875411 23.483651329013092 92-93 22.211567005253034 27.170071890447733 28.01985054014636 22.598510564152868 94-95 21.819887744421745 26.89113480348821 28.018207653460912 23.270769798629136 96-97 21.16142462684304 27.002490186702698 28.598300426603373 23.237784759850886 98-99 21.62434785751348 27.42999713457433 27.38365984557679 23.5619951623354 100-101 21.4512969304205 26.854092503719578 27.791006529028845 23.90360403683108 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 93.0 1 89.0 2 218.5 3 318.5 4 221.5 5 173.0 6 315.0 7 707.0 8 1149.5 9 1315.5 10 1120.5 11 918.0 12 956.0 13 1079.0 14 1158.5 15 1204.5 16 1260.0 17 1298.5 18 1368.5 19 1518.5 20 1635.5 21 1802.5 22 2096.5 23 2439.5 24 2994.5 25 3758.5 26 4624.5 27 5589.5 28 6429.0 29 7387.5 30 8806.5 31 10449.0 32 12139.0 33 13270.0 34 14683.0 35 16140.0 36 17407.0 37 18693.0 38 19617.0 39 20106.5 40 20415.5 41 21783.5 42 23081.5 43 24004.5 44 25073.5 45 26325.5 46 27256.5 47 31540.5 48 43226.5 49 42965.5 50 32255.5 51 27852.5 52 25566.5 53 24694.0 54 23911.0 55 22024.5 56 19640.0 57 17413.5 58 15919.5 59 14156.5 60 11961.5 61 10081.0 62 8477.5 63 6728.0 64 5051.0 65 4015.5 66 3047.5 67 2399.5 68 1845.0 69 1249.5 70 951.0 71 763.5 72 620.5 73 515.0 74 418.0 75 364.0 76 267.5 77 161.5 78 115.0 79 78.5 80 46.5 81 37.5 82 28.0 83 17.5 84 11.0 85 9.5 86 10.5 87 8.0 88 4.0 89 4.0 90 4.5 91 2.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028906657306415732 2 0.019357136589117675 3 0.017292375352945123 4 0.047360460854707916 5 0.05213522121335694 6 0.045424747195796145 7 0.048392841472794185 8 0.04206951018701575 9 0.03974665379632163 10-11 0.04858641283868537 12-13 0.04219855776427653 14-15 0.036068797844389267 16-17 0.0424566529187981 18-19 0.04813474631827262 20-21 0.010452853758123545 22-23 0.017937613239249046 24-25 0.006129759919887264 26-27 0.010194758603601977 28-29 0.006387855074408833 30-31 0.008452616310581385 32-33 0.004129522472345104 34-35 0.008065473578799031 36-37 0.009484996928667661 38-39 0.010646425124014721 40-41 0.002064761236172552 42-43 0.0021292850248029443 44-45 0.0019357136589117675 46-47 3.2261894315196125E-4 48-49 8.388092521950993E-4 50-51 2.58095154521569E-4 52-53 0.003032618065628436 54-55 0.006129759919887264 56-57 0.014259757287316687 58-59 0.009484996928667661 60-61 0.009936663449080407 62-63 0.005355474456322557 64-65 6.452378863039225E-5 66-67 0.005290950667692165 68-69 0.015808328214446103 70-71 0.005871664765365696 72-73 0.0018711898702813755 74-75 0.0036133321633019665 76-77 0.005290950667692165 78-79 0.004645712781388242 80-81 0.010839996489905898 82-83 0.006258807497148048 84-85 0.01458237623046865 86-87 0.003935951106453927 88-89 0.005226426879061772 90-91 0.0036778559519323587 92-93 0.015227614116772571 94-95 0.00890428283099413 96-97 0.008581663887842171 98-99 0.019873326898160813 100-101 0.0374883211942579 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 774908.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.8029031733388 #Duplication Level Percentage of deduplicated Percentage of total 1 85.8436278510304 55.62916303678453 2 8.666593377681208 11.232408229931492 3 2.235687903557668 4.346372002201572 4 0.9603498007631048 2.4893382060554683 5 0.5512015920597022 1.785973169961754 6 0.3400328983250296 1.322107139154399 7 0.2357831633357328 1.0695603452484315 8 0.1677585034973983 0.8696990446916898 9 0.12851168617808303 0.7495137320436736 >10 0.7811883098560849 9.445074913164762 >50 0.05618954284940215 2.5092202426315025 >100 0.027695641875769384 3.1311425502453027 >500 0.002988738328031117 1.3786065559897869 >1k 0.0021917414405561524 3.359038077556833 >5k 1.9924922186874113E-4 0.6827827543386712 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5288 0.6824035885550285 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4629 0.5973612351401715 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4001 0.5163193566203989 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3016 0.38920749301852603 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2910 0.37552844982888295 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2838 0.36623702426610644 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1977 0.255127060244571 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1748 0.2255751650518513 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1400 0.1806666081650983 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1335 0.1722785156431473 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1140 0.14711423807729432 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1021 0.131757576383261 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 986 0.12724091117913353 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 931 0.12014329442979037 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 857 0.11059377371249232 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 821 0.1059480609311041 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 1.290475772607845E-4 10-11 0.0 0.0 0.0 0.0 1.290475772607845E-4 12-13 0.0 0.0 0.0 0.0 1.290475772607845E-4 14-15 0.0 0.0 0.0 0.0 1.290475772607845E-4 16-17 0.0 0.0 0.0 0.0 1.290475772607845E-4 18-19 0.0 0.0 0.0 0.0 1.290475772607845E-4 20-21 0.0 0.0 0.0 0.0 1.290475772607845E-4 22-23 0.0 0.0 0.0 0.0 1.290475772607845E-4 24-25 0.0 0.0 0.0 3.871427317823535E-4 1.290475772607845E-4 26-27 0.0 0.0 0.0 9.033330408254915E-4 1.290475772607845E-4 28-29 0.0 0.0 0.0 0.0020002374475421598 1.290475772607845E-4 30-31 0.0 0.0 0.0 0.0042585700496058886 1.290475772607845E-4 32-33 0.0 0.0 0.0 0.008452616310581385 1.290475772607845E-4 34-35 0.0 0.0 0.0 0.01348547182375198 1.290475772607845E-4 36-37 0.0 0.0 0.0 0.023615706638723564 1.290475772607845E-4 38-39 0.0 0.0 0.0 0.03942403485316967 1.290475772607845E-4 40-41 0.0 0.0 0.0 0.05407093487226871 1.290475772607845E-4 42-43 0.0 0.0 0.0 0.07839640318592658 1.290475772607845E-4 44-45 0.0 0.0 0.0 0.10988401203755802 1.290475772607845E-4 46-47 0.0 0.0 0.0 0.1466625715568816 1.290475772607845E-4 48-49 0.0 0.0 0.0 0.19105493813459146 1.290475772607845E-4 50-51 0.0 0.0 0.0 0.241318969477667 1.290475772607845E-4 52-53 0.0 0.0 0.0 0.29687395148843476 1.290475772607845E-4 54-55 0.0 0.0 0.0 0.35610678945113483 1.290475772607845E-4 56-57 0.0 0.0 0.0 0.4386972388980369 1.290475772607845E-4 58-59 0.0 0.0 0.0 0.5354829218436252 1.290475772607845E-4 60-61 0.0 0.0 0.0 0.6434312202222716 1.290475772607845E-4 62-63 0.0 0.0 0.0 0.7618968961476718 1.290475772607845E-4 64-65 0.0 0.0 0.0 0.8796528103981376 1.290475772607845E-4 66-67 0.0 0.0 0.0 1.0334775224929929 1.290475772607845E-4 68-69 0.0 0.0 0.0 1.2133698451945265 1.290475772607845E-4 70-71 0.0 0.0 0.0 1.4003597846454032 1.290475772607845E-4 72-73 0.0 0.0 0.0 1.6121268589303503 1.290475772607845E-4 74-75 0.0 0.0 0.0 1.8192482204339095 1.290475772607845E-4 76-77 0.0 0.0 0.0 2.038887196931765 1.290475772607845E-4 78-79 0.0 0.0 0.0 2.304079968202677 1.290475772607845E-4 80-81 0.0 0.0 0.0 2.62857010122492 1.290475772607845E-4 82-83 0.0 0.0 0.0 2.956092852312791 1.290475772607845E-4 84-85 0.0 0.0 0.0 3.3161355928703795 1.290475772607845E-4 86-87 0.0 0.0 0.0 3.711859988540575 1.290475772607845E-4 88-89 0.0 0.0 0.0 4.1566869873584995 1.290475772607845E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 60 0.003941092 31.681541 2 TCCCGTA 60 0.003941092 31.681541 2 CGTCATA 40 4.4854032E-4 29.697609 14-15 TACCTGG 660 0.0 29.521435 2 GTACCTG 695 0.0 28.718521 1 GTACATG 3565 0.0 27.5936 1 CCAACGC 70 0.008370713 27.155607 3 TACATGG 3790 0.0 25.328514 2 ACATGGG 3555 0.0 24.997671 3 GAGTACT 3165 0.0 23.872225 12-13 GGACCGT 100 0.0016486476 23.761156 6 ACCGTCC 125 2.2478214E-4 22.810709 8 TATCGGC 105 0.0021895957 22.629671 2 AGTACTT 3295 0.0 22.209297 12-13 GTACTTT 3475 0.0 21.809582 14-15 GTCTAGG 275 1.2496457E-9 20.73701 1 CATGGGG 1495 0.0 20.345314 4 CATGGGT 610 0.0 20.25672 4 CATGGGA 2050 0.0 19.937477 4 GTATCGG 120 0.0047492012 19.800964 1 >>END_MODULE