FastQCFastQC Report
Thu 26 May 2016
SRR936397_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936397_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences774908
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT90631.16955819271449No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35620.45966747020291443No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32440.41863034063398497No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24470.3157794215571397No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21250.27422610167916706No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT19750.2548689650900494No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC17010.21950992892059445No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC16440.21215421701672976No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT15670.20221755356764934No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG14620.18866755795526696No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA14600.1884094628007454No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT14220.18350565486483558No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA13880.1791180372379689No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC13720.17705327600179635No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12110.15627661606281004No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA10590.1366613843191708No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10020.12930567241530608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGCG801.9081199E-929.68532294-95
ACTGCGT856.408028E-427.9391298
TACCTGG5900.025.7641472
TAACGCA950.001227179924.9965534
GTACCTG7000.024.4346641
ATACGTC1401.7804101E-523.7467255
TAAGGTG3600.022.4274645
GAGTACT40800.021.77274712-13
GGTGTGC7250.021.6191048
TCGTACA1100.002875599321.5921122
GACCCCG1100.00287775421.5893257
GTAAGGT3101.0913936E-1121.4486564
AGCACCG1353.797939E-421.10825
GTACTTT42850.020.73044214-15
GTACATG34850.020.4498621
TAATACG1707.946183E-519.5599143
GGTAAGG3404.1836756E-1119.5599143
GTATTAG1951.0297159E-519.4920391
TAGACAG3900.019.4844935
CTACACT3203.965397E-1019.2942144
Produced by FastQC (version 0.11.5)