##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936397_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 774908 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.600657110263413 31.0 31.0 34.0 30.0 34.0 2 31.7458072442148 31.0 31.0 34.0 30.0 34.0 3 31.848259406277908 31.0 31.0 34.0 30.0 34.0 4 35.6290940343886 37.0 35.0 37.0 33.0 37.0 5 35.38113685753664 37.0 35.0 37.0 33.0 37.0 6 35.52013400300423 37.0 35.0 37.0 33.0 37.0 7 35.459735865418864 37.0 35.0 37.0 33.0 37.0 8 35.43975800998312 37.0 35.0 37.0 33.0 37.0 9 37.00660336452844 39.0 37.0 39.0 33.0 39.0 10-11 36.846515328271224 39.0 37.0 39.0 33.0 39.0 12-13 36.73673971619857 39.0 35.0 39.0 32.0 39.0 14-15 37.703068622339686 39.0 37.0 41.0 32.0 41.0 16-17 37.645151424427155 39.0 37.0 41.0 32.0 41.0 18-19 37.83183035921684 39.5 37.0 41.0 33.0 41.0 20-21 37.86362832749178 40.0 37.0 41.0 33.0 41.0 22-23 37.75681887398246 39.0 37.0 41.0 32.0 41.0 24-25 37.59316138173822 39.0 36.5 41.0 32.0 41.0 26-27 37.31167054669716 39.0 36.0 40.5 31.5 41.0 28-29 37.2119154789988 39.0 36.0 40.0 31.5 41.0 30-31 37.19914299503941 39.0 36.0 40.0 31.0 41.0 32-33 37.02653282712271 39.0 36.0 40.0 31.0 41.0 34-35 36.8098593639503 39.0 35.0 40.0 30.5 41.0 36-37 36.53125532321256 38.5 35.0 40.0 30.0 41.0 38-39 36.32210920522178 38.0 35.0 40.0 30.0 41.0 40-41 36.083507332483336 38.0 35.0 40.0 30.0 41.0 42-43 35.79516407108973 38.0 35.0 40.0 29.0 41.0 44-45 35.503204251343384 38.0 34.5 40.0 27.5 41.0 46-47 35.072719600262225 38.0 34.0 40.0 26.5 41.0 48-49 34.63722919365912 38.0 33.5 40.0 24.5 41.0 50-51 34.26519148079514 38.0 33.0 40.0 24.0 41.0 52-53 33.96510811606023 38.0 33.0 40.0 23.0 41.0 54-55 33.689023729268506 37.0 33.0 40.0 23.0 41.0 56-57 33.31534001455657 37.0 32.0 40.0 21.5 41.0 58-59 32.92885542541824 36.5 31.5 39.0 20.5 40.0 60-61 32.4807454562348 36.0 31.0 39.0 19.0 40.0 62-63 32.072344071812395 35.5 31.0 39.0 18.0 40.0 64-65 31.62908306534453 35.0 30.0 38.0 15.5 40.0 66-67 31.08939770914741 35.0 29.5 38.0 12.5 40.0 68-69 30.75151888998436 34.0 29.0 37.5 9.5 39.5 70-71 30.241875164535664 34.0 29.0 37.0 7.5 39.0 72-73 29.71962090983704 34.0 28.5 36.0 7.0 39.0 74-75 29.160160948138362 33.5 27.5 36.0 4.0 38.5 76-77 27.90695734203286 31.5 26.0 34.5 2.0 36.5 78-79 28.683850857134008 33.0 28.0 35.0 2.0 37.0 80-81 28.428872201603287 33.0 27.0 35.0 2.0 37.0 82-83 28.019503605589307 33.0 26.5 35.0 2.0 36.0 84-85 27.513935202630506 33.0 26.0 35.0 2.0 36.0 86-87 27.08254721850852 32.0 25.5 35.0 2.0 35.0 88-89 26.55903926659681 32.0 25.0 34.0 2.0 35.0 90-91 25.92135518022785 32.0 23.5 34.0 2.0 35.0 92-93 25.299976255245785 31.0 21.0 34.0 2.0 35.0 94-95 24.531659887367276 31.0 18.5 34.0 2.0 35.0 96-97 23.52635151527665 31.0 4.5 34.0 2.0 35.0 98-99 22.33237429475499 30.0 2.0 34.0 2.0 35.0 100-101 19.075531546970737 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2733218261412098 1101 2 0.33164409611809376 1101 3 0.3140264788886675 1101 4 0.07726402164345103 1101 5 0.08093769870159662 1101 6 0.21289951511763405 1101 7 0.11791176920829116 1101 8 0.22515463351776077 1101 9 0.27203260983451827 1101 10-11 0.2540506537677558 1101 12-13 0.22569532118502877 1101 14-15 0.5406808526807225 1101 16-17 0.3799865915360172 1101 18-19 0.3661085665452575 1101 20-21 0.23375716964540771 1101 22-23 0.24471877196571512 1101 24-25 0.020928836852817767 1101 26-27 0.19299307212450145 1101 28-29 0.1936394674388353 1101 30-31 0.4920541644545793 1101 32-33 0.42503747414644266 1101 34-35 0.15648476525749544 1101 36-37 0.3122501778101068 1101 38-39 0.11375357770107541 1101 40-41 0.28475405182710745 1101 42-43 0.4205122583968972 1101 44-45 0.49075308204685086 1101 46-47 0.2833189191214984 1101 48-49 0.37761042960866575 1101 50-51 0.3975575060417569 1101 52-53 0.4653770992356172 1101 54-55 0.4452911847799754 1101 56-57 0.3819692162070396 1101 58-59 0.3187408675302521 1101 60-61 0.3948307937574782 1101 62-63 0.5193286863720061 1101 64-65 0.5014384739855124 1101 66-67 0.34031031075497964 1101 68-69 0.2788713033875858 1101 70-71 0.3305451174750722 1101 72-73 0.3839063321488858 1101 74-75 0.48698016452221893 1101 76-77 0.379557175132625 1101 78-79 0.2443009610647522 1101 80-81 0.13435498615978148 1101 82-83 0.37543677600033476 1101 84-85 0.33378898182184 1101 86-87 0.4150839534759818 1101 88-89 0.09186496181623482 1101 90-91 0.24633599447182775 1101 92-93 0.13728410219806975 1101 94-95 0.13655369017413221 1101 96-97 0.09722147407680026 1101 98-99 0.4575216801132065 1101 100-101 0.8605380033443879 1102 1 0.3010919389010418 1102 2 0.3124152859365772 1102 3 0.23993097573216815 1102 4 0.18702707863062784 1102 5 0.1950569584685624 1102 6 0.27533331161586005 1102 7 0.24249699283395643 1102 8 0.30335017949946064 1102 9 0.3202717821312646 1102 10-11 0.3678860606120722 1102 12-13 0.2714695720655129 1102 14-15 0.5160813375054758 1102 16-17 0.3602318622199405 1102 18-19 0.29596400925197486 1102 20-21 0.2927941269003256 1102 22-23 0.30717877788014647 1102 24-25 0.4513411060245076 1102 26-27 0.13602690600823308 1102 28-29 0.00273997016511629 1102 30-31 0.29447274960106284 1102 32-33 0.34400537700741296 1102 34-35 0.38495916953723963 1102 36-37 0.6270111726601968 1102 38-39 0.554636508645693 1102 40-41 0.337107360691725 1102 42-43 0.21613024181474572 1102 44-45 0.34106986982080656 1102 46-47 0.4108314378266158 1102 48-49 0.10835714082144676 1102 50-51 0.1380504112022436 1102 52-53 0.00625760704552647 1102 54-55 0.3173128307906552 1102 56-57 0.37460906816834694 1102 58-59 -0.005914429194959325 1102 60-61 0.40102412825787326 1102 62-63 0.2904828996098274 1102 64-65 0.48669988076850856 1102 66-67 0.3737965488334005 1102 68-69 0.15967359834341366 1102 70-71 0.05994278552935128 1102 72-73 0.14313872263555893 1102 74-75 -0.11592467947241758 1102 76-77 -0.41482871846505986 1102 78-79 -0.34232387932979336 1102 80-81 0.0038315989537345274 1102 82-83 -0.1665472471351599 1102 84-85 0.014080836034189304 1102 86-87 0.21527622179710093 1102 88-89 -0.07788600916845922 1102 90-91 0.10322664779839386 1102 92-93 0.09652920177901692 1102 94-95 0.13844694960680215 1102 96-97 0.49987179002389226 1102 98-99 0.4678429976655032 1102 100-101 0.7531335465567892 1103 1 0.09970499650690456 1103 2 0.21757168030160656 1103 3 0.20060659108404266 1103 4 0.1631500763363647 1103 5 -0.05474320140353939 1103 6 0.19154579617367062 1103 7 0.27738336944695163 1103 8 0.11464400158465082 1103 9 0.1429279429169199 1103 10-11 0.1222643761414659 1103 12-13 0.1068661898362393 1103 14-15 0.042359016660846294 1103 16-17 0.3539849117283822 1103 18-19 0.11136623292757264 1103 20-21 0.09021333439147128 1103 22-23 -0.00635740615963698 1103 24-25 0.0739624894372568 1103 26-27 0.07569335466748583 1103 28-29 0.3745464211082492 1103 30-31 -0.015954233487789793 1103 32-33 0.17034171053799696 1103 34-35 0.26606207941713933 1103 36-37 0.3305184966711252 1103 38-39 0.4036559943558231 1103 40-41 0.3404008298125376 1103 42-43 0.34324932576836176 1103 44-45 0.4076373950214247 1103 46-47 0.3781069680517817 1103 48-49 0.30019847295055513 1103 50-51 0.6484550744846018 1103 52-53 0.29087406971954266 1103 54-55 0.3413948638654958 1103 56-57 0.5127868376410802 1103 58-59 0.542143228574929 1103 60-61 0.3299069959896599 1103 62-63 0.07018121887649542 1103 64-65 0.0760258226124364 1103 66-67 0.33760212774689435 1103 68-69 0.3221488129769021 1103 70-71 0.49753037779320053 1103 72-73 0.37171430372912795 1103 74-75 0.3465858315992456 1103 76-77 0.28850212335499137 1103 78-79 0.29223081457741884 1103 80-81 0.5224245168272255 1103 82-83 0.6313910256232553 1103 84-85 0.13279884863709412 1103 86-87 0.11178498880946819 1103 88-89 -0.09027609709811557 1103 90-91 -0.20741834270270942 1103 92-93 0.15568966561573916 1103 94-95 0.572268723356629 1103 96-97 0.7171940361231144 1103 98-99 1.0040763486470574 1103 100-101 0.9274278773259397 1104 1 0.05864680233825936 1104 2 0.11896821013656123 1104 3 0.05585361518421905 1104 4 -0.06689285764224451 1104 5 -0.1529068424364155 1104 6 -0.009405479005280881 1104 7 -0.05326541469315771 1104 8 -0.045877716787259715 1104 9 -0.13411304872415997 1104 10-11 0.08906161708174665 1104 12-13 -0.06459338458567743 1104 14-15 0.012092189398678954 1104 16-17 0.11547237334280425 1104 18-19 -0.05934244899565755 1104 20-21 -0.2213766696234103 1104 22-23 -0.18374924834200357 1104 24-25 -0.022557217873817592 1104 26-27 0.1218146476783275 1104 28-29 0.03268127069464555 1104 30-31 -0.21492700097515893 1104 32-33 -0.1566325471599228 1104 34-35 -0.08839100969528602 1104 36-37 -0.015015754660844038 1104 38-39 0.006328607396618224 1104 40-41 -0.1467052319443951 1104 42-43 -0.16292926077417036 1104 44-45 0.058717255115247724 1104 46-47 0.09693389745848435 1104 48-49 0.18692040762000772 1104 50-51 -0.02821698153573493 1104 52-53 0.0640354501174798 1104 54-55 0.16408729815335477 1104 56-57 0.3758610841262424 1104 58-59 0.02419957444845977 1104 60-61 0.11559844283218723 1104 62-63 0.09014859339317383 1104 64-65 0.22854561673189266 1104 66-67 -0.04798297882847535 1104 68-69 0.3202506030380654 1104 70-71 0.20863461657412685 1104 72-73 -0.11035614850636932 1104 74-75 0.07803397625094632 1104 76-77 -0.15297455955207795 1104 78-79 0.12695814583458898 1104 80-81 0.25034064546278145 1104 82-83 0.13952641776203123 1104 84-85 0.08929157300963197 1104 86-87 0.2342748702075177 1104 88-89 0.05620147197784675 1104 90-91 0.16186017113780338 1104 92-93 0.12511466859977816 1104 94-95 -0.023783303799923772 1104 96-97 0.42910595455217404 1104 98-99 0.3673412118696042 1104 100-101 0.8328946351414075 1105 1 0.08209645004701116 1105 2 0.06264100025369501 1105 3 0.1195023776104236 1105 4 -0.02148741497635598 1105 5 0.06393617500827986 1105 6 0.13456765724846775 1105 7 0.11061623908160101 1105 8 0.09837410129161128 1105 9 -0.06472225901076456 1105 10-11 -0.1491724461091124 1105 12-13 -0.09518979323367205 1105 14-15 -0.09721115527559476 1105 16-17 0.11057180760884933 1105 18-19 0.19978733500130375 1105 20-21 0.08784395677061241 1105 22-23 0.003518044948464194 1105 24-25 -0.05613887017145913 1105 26-27 -0.0634240782632105 1105 28-29 0.033797475107270714 1105 30-31 0.1991459388226744 1105 32-33 0.13640237669495292 1105 34-35 0.2763572165392105 1105 36-37 0.10646096358986057 1105 38-39 0.3715519713718223 1105 40-41 0.17127171525869045 1105 42-43 -0.22206643033187845 1105 44-45 -0.004967450373207782 1105 46-47 -0.01005432011029228 1105 48-49 -0.003922974179019434 1105 50-51 -0.0353056427305205 1105 52-53 0.04333716996840309 1105 54-55 0.2849155641714063 1105 56-57 0.025512239474124954 1105 58-59 0.04049753751080942 1105 60-61 0.205805189295738 1105 62-63 0.13868354432842978 1105 64-65 -0.05629182707630065 1105 66-67 0.12355356246419191 1105 68-69 0.38430409419262546 1105 70-71 0.23077751240325384 1105 72-73 0.05616608941700818 1105 74-75 -0.36506421724089577 1105 76-77 -0.40778132866467587 1105 78-79 -0.2735563815633846 1105 80-81 0.10227837041464838 1105 82-83 -0.09566824100044258 1105 84-85 -0.33138258097438467 1105 86-87 -0.27313580143537664 1105 88-89 0.1377469251804726 1105 90-91 0.23742578199752984 1105 92-93 0.06116506766624141 1105 94-95 0.10045118282975096 1105 96-97 0.31490302888538224 1105 98-99 0.6346034829787648 1105 100-101 0.9330812436426825 1106 1 0.007141531108459276 1106 2 0.1615068297241109 1106 3 0.02145159683881559 1106 4 0.13225836021378967 1106 5 -0.050091812553432646 1106 6 0.03706446532631702 1106 7 0.05277030440093 1106 8 -0.017734442361359015 1106 9 -0.013554374463332408 1106 10-11 0.05157939760423602 1106 12-13 0.18364202660536932 1106 14-15 0.1565765688803893 1106 16-17 0.2152358850764884 1106 18-19 -0.08316688572626418 1106 20-21 0.0339592432271516 1106 22-23 0.18841303880392246 1106 24-25 0.16423748806708005 1106 26-27 0.07066762104998503 1106 28-29 0.24400402449514047 1106 30-31 0.06659706053520864 1106 32-33 0.1263613708769995 1106 34-35 0.36669670754903905 1106 36-37 0.18404473131084131 1106 38-39 0.4411070167203448 1106 40-41 0.3543958433747818 1106 42-43 0.3971270515308305 1106 44-45 0.27601953277822844 1106 46-47 0.0619149022994705 1106 48-49 0.23530859959696926 1106 50-51 0.25634806151799694 1106 52-53 0.18525558908361717 1106 54-55 -0.10857840460898416 1106 56-57 -0.08308420162065033 1106 58-59 0.13031900102295424 1106 60-61 0.04677567439752295 1106 62-63 -0.09667830773248909 1106 64-65 0.41811437686605757 1106 66-67 0.2984272580695446 1106 68-69 0.2878967838110995 1106 70-71 0.5015721637693389 1106 72-73 0.2971002562866154 1106 74-75 0.5571211549576383 1106 76-77 -0.06171291806594681 1106 78-79 0.19833077290312673 1106 80-81 0.18313992897357778 1106 82-83 0.7857534347903581 1106 84-85 0.44760056483205446 1106 86-87 0.49484981767082914 1106 88-89 0.2828298166185199 1106 90-91 0.180504695322238 1106 92-93 0.0966295398483048 1106 94-95 0.49110726184020237 1106 96-97 0.6705669135003873 1106 98-99 1.0064852882482889 1106 100-101 1.379221164559027 1107 1 0.07648824771023754 1107 2 0.21941745904853605 1107 3 0.13278209173364175 1107 4 0.12312863287061049 1107 5 0.13061781765710379 1107 6 0.15903910462952808 1107 7 0.10596632647035165 1107 8 0.05034794439698942 1107 9 0.3972123435586994 1107 10-11 0.14528414648270882 1107 12-13 0.17730339615710022 1107 14-15 0.316926474866122 1107 16-17 0.49003311373956393 1107 18-19 0.23027730931706714 1107 20-21 0.21080564336052987 1107 22-23 0.11588465699175288 1107 24-25 0.21001116839385503 1107 26-27 0.38461662178171707 1107 28-29 0.24035305980684285 1107 30-31 0.2561531402790749 1107 32-33 0.33584415345239904 1107 34-35 0.24217951482441435 1107 36-37 0.4077593293033459 1107 38-39 0.5513182941102954 1107 40-41 0.3851612028983098 1107 42-43 0.23428399206873962 1107 44-45 0.47431245592735394 1107 46-47 0.630333826183417 1107 48-49 0.4081312198318017 1107 50-51 0.5475916125095495 1107 52-53 0.5035239975100083 1107 54-55 0.6920179453525677 1107 56-57 0.5866570970421492 1107 58-59 0.5893417466101454 1107 60-61 0.7257416409228767 1107 62-63 0.6072346310393684 1107 64-65 0.8607317094542815 1107 66-67 0.7241058126271547 1107 68-69 0.6886584692137241 1107 70-71 0.4516496599331248 1107 72-73 0.44354562149114685 1107 74-75 0.6220241921280056 1107 76-77 0.3877926193591428 1107 78-79 0.6083850510927427 1107 80-81 0.6579228007776301 1107 82-83 0.9470210991300796 1107 84-85 1.0194852036103939 1107 86-87 0.9666710083947514 1107 88-89 0.8674202756366363 1107 90-91 1.069168025434287 1107 92-93 1.0988039093043191 1107 94-95 1.329029901526269 1107 96-97 1.2706782177515628 1107 98-99 1.2963031877008753 1107 100-101 1.341793759322016 1108 1 -0.0305437456758213 1108 2 0.184327687260744 1108 3 0.025687702665365464 1108 4 0.14793871601285957 1108 5 -0.06638095002816868 1108 6 6.74963428103581E-4 1108 7 -0.020108049182852028 1108 8 -0.05799435831566768 1108 9 0.13119615187915912 1108 10-11 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0.21940796664171103 2316 62-63 0.548347257474191 2316 64-65 -0.059043264974636855 2316 66-67 -0.6629711279342736 2316 68-69 -0.04931630064648829 2316 70-71 0.3814723670589366 2316 72-73 0.7306134701103062 2316 74-75 0.767350933803197 2316 76-77 1.0393848457485042 2316 78-79 0.6086862216803084 2316 80-81 0.12836262348475813 2316 82-83 0.5706533300654151 2316 84-85 0.5502568822102347 2316 86-87 0.4539775204984018 2316 88-89 0.5887806574982086 2316 90-91 0.14885435342610265 2316 92-93 0.6069332421830573 2316 94-95 0.5106403118001595 2316 96-97 0.69713008728219 2316 98-99 0.5086943685168741 2316 100-101 -0.13458651116326692 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 14.0 5 34.0 6 84.0 7 189.0 8 419.0 9 860.0 10 1261.0 11 1982.0 12 2781.0 13 3891.0 14 4964.0 15 6396.0 16 7901.0 17 9965.0 18 8997.0 19 6553.0 20 6576.0 21 6791.0 22 7251.0 23 8181.0 24 9451.0 25 11210.0 26 13751.0 27 16728.0 28 20275.0 29 24368.0 30 29411.0 31 35259.0 32 43066.0 33 53210.0 34 67315.0 35 87186.0 36 111368.0 37 116716.0 38 48843.0 39 1656.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.783910782128025 19.50627579414001 14.943399733812864 21.766413689919098 2 16.14281603002306 21.999790932942386 38.12750767547114 23.729885361563408 3 21.521359270912107 24.609426418853868 29.642166718717334 24.227047591516687 4 13.391800564454831 17.197547576676943 37.15349067694575 32.25716118192248 5 13.344534865413852 38.45777616035225 33.682026186807484 14.51566278742641 6 31.481706121016277 37.13079297564602 16.371318253615932 15.01618264972177 7 28.84742446845303 33.288209697151146 19.869068328111208 17.995297506284615 8 26.578768060208436 33.86518141508411 18.726997269353266 20.829053255354186 9 25.934959349593495 17.970705897535165 20.43644341205317 35.65789134081817 10-11 25.70332951135206 26.424580609069388 26.538143136532483 21.33394674304607 12-13 27.563663299767583 23.748511092441962 26.99997548061226 21.687850127178194 14-15 22.972060031475294 25.71192041228891 26.90266468876046 24.413354867475338 16-17 21.672082531062713 28.85406073683287 27.27427589161733 22.199580840487094 18-19 21.401508308083024 27.993258554563898 29.60564350864877 20.99958962870431 20-21 22.668239326474886 25.9067528016229 29.477628312006072 21.947379559896145 22-23 21.92776541763239 26.269660804344348 29.526759858147972 22.275813919875286 24-25 22.955105791138077 26.319606607050332 29.64758655799881 21.077701043812777 26-27 22.438200963903 25.953691616857267 30.543039127183185 21.065068292056544 28-29 21.306936238639853 27.655325233784772 29.969813823952258 21.067924703623113 30-31 21.894724344721052 26.144762091575807 29.827822722703218 22.132690840999924 32-33 21.839880707726834 26.717455786430182 29.728475868285535 21.71418763755745 34-35 22.83756829640203 26.020227414838814 29.68918079163491 21.453023497124242 36-37 22.834023720761248 25.755591880037144 30.15617447711138 21.254209922090226 38-39 22.021386226526744 26.577074496882926 30.22612924270612 21.17541003388422 40-41 21.959182830695017 26.790033998176526 29.65397225041602 21.596810920712432 42-43 21.717105237687814 27.10700565102987 28.783989738070442 22.391899373211874 44-45 22.504221493385952 26.440518077412218 28.660988110132422 22.394272319069405 46-47 23.07399468581391 27.307922111943757 28.18480153102243 21.433281671219902 48-49 23.093247679712583 27.261505941381124 28.063540774082984 21.581705604823306 50-51 22.00922823393076 27.59133544843268 28.751801028384786 21.64763528925177 52-53 22.758329660799863 27.997158330176998 27.61672042653438 21.627791582488758 54-55 21.80614139177263 27.31841120572675 28.114008513471923 22.761438889028693 56-57 22.969935946201776 27.134239390988853 27.889889668994076 22.005934993815295 58-59 23.701129430226196 26.91165615353644 27.51573098086989 21.871483435367477 60-61 22.386712368012844 27.16477004359351 29.062855697483243 21.385661890910406 62-63 22.115510565163834 27.273331470682937 28.769753826875466 21.841404137277763 64-65 21.866788669719327 28.296986232067354 28.022543892706143 21.813681205507173 66-67 22.266099615879227 27.4067879618499 27.506092827788326 22.821019594482546 68-69 21.909775231743467 27.593120029113923 27.90471384859986 22.59239089054275 70-71 22.83967903286584 27.42622350008001 27.602179871675087 22.131917595379065 72-73 22.704812467092697 26.813450440312607 28.479592310789464 22.00214478180523 74-75 21.913603349215606 28.628166345751794 27.49145660938379 21.966773695648808 76-77 22.44148546960677 27.329708746885057 27.421398825386216 22.80740695812196 78-79 22.444375904161756 27.644346480592702 27.589758016954686 22.321519598290852 80-81 22.681526611557025 27.837489570959097 27.458462896489067 22.02252092099481 82-83 22.461318478665557 27.42018014274367 28.003943808472346 22.114557570118425 84-85 21.88491290196858 27.99248652038912 27.757157937935716 22.365442639706583 86-87 22.035922657110003 28.113970165972585 27.150087690205154 22.700019486712257 88-89 21.828545174489626 28.0058411644644 26.837801858564063 23.327811802481914 90-91 22.389280021682595 27.368984854449124 27.127314262114183 23.114420861754095 92-93 22.633357443955706 27.934554178246113 27.295375341737422 22.13671303606076 94-95 22.242174746255944 27.794410855664314 27.19665244968673 22.766761948393007 96-97 21.55200170344011 27.864408748197356 27.81440245708331 22.769187091279225 98-99 22.01847885913467 28.270047639296386 26.56160168179686 23.149871819772084 100-101 21.561449831973114 27.95944125253589 26.85771658949303 23.621392325997967 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 165.0 1 131.5 2 214.5 3 323.5 4 253.0 5 198.5 6 338.0 7 676.5 8 993.0 9 1163.0 10 1113.5 11 962.0 12 931.5 13 1027.0 14 1148.0 15 1259.0 16 1364.5 17 1457.5 18 1546.0 19 1677.0 20 1808.5 21 1957.5 22 2204.0 23 2584.5 24 3176.0 25 3955.5 26 4814.5 27 5682.5 28 6631.5 29 7781.5 30 9082.0 31 10533.5 32 12240.5 33 13500.5 34 14793.5 35 16086.0 36 17508.0 37 18848.0 38 19677.5 39 20106.5 40 20442.5 41 21802.5 42 23208.5 43 24343.5 44 25414.0 45 26493.0 46 27101.0 47 31759.0 48 43801.0 49 42876.0 50 31759.5 51 27352.0 52 25072.0 53 24232.0 54 23370.0 55 21569.5 56 19268.0 57 17081.0 58 15688.5 59 13970.0 60 11812.0 61 9970.0 62 8430.5 63 6755.0 64 5034.0 65 3786.5 66 2859.0 67 2383.0 68 1834.0 69 1229.5 70 891.0 71 720.5 72 618.5 73 512.5 74 431.5 75 355.5 76 247.5 77 178.5 78 126.0 79 81.5 80 60.5 81 50.0 82 38.5 83 26.0 84 17.0 85 10.5 86 6.0 87 3.0 88 1.0 89 1.0 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.034197607974107896 2 0.0047747603586490266 3 0.002839046699737259 4 1.290475772607845E-4 5 0.0018066660816509832 6 5.16190309043138E-4 7 0.0 8 0.0 9 0.001032380618086276 10-11 5.807140976735303E-4 12-13 0.0014195233498686295 14-15 0.0018711898702813755 16-17 0.003097141854258828 18-19 0.0 20-21 0.0 22-23 0.002064761236172552 24-25 0.0010969044067166683 26-27 0.004839284147279419 28-29 0.006452378863039225 30-31 0.0011614281953470605 32-33 5.807140976735303E-4 34-35 0.008839759042363738 36-37 0.013098329091969629 38-39 0.008194521156059816 40-41 0.0017421422930205908 42-43 6.452378863039225E-4 44-45 5.807140976735303E-4 46-47 1.290475772607845E-4 48-49 5.16190309043138E-4 50-51 4.516665204127458E-4 52-53 0.0014840471384990218 54-55 0.0023873801793245133 56-57 0.0016130947157598063 58-59 5.16190309043138E-4 60-61 0.002839046699737259 62-63 0.0034197607974107893 64-65 0.008259044944690208 66-67 0.002774522911106867 68-69 0.0027099991224764745 70-71 0.0 72-73 5.16190309043138E-4 74-75 0.00516190309043138 76-77 0.0019357136589117675 78-79 0.0024519039679549055 80-81 0.0036778559519323587 82-83 0.0029035704883676513 84-85 0.004387617626866673 86-87 0.0027099991224764745 88-89 0.008065473578799031 90-91 0.012001424685252959 92-93 9.678568294558838E-4 94-95 0.0016776185043901985 96-97 7.097616749343148E-4 98-99 0.0029035704883676513 100-101 0.004129522472345104 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 774908.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.642485800802774 #Duplication Level Percentage of deduplicated Percentage of total 1 85.41204806933305 54.35835056470013 2 8.826703777452275 11.235067396487972 3 2.3588145158384743 4.503624579929328 4 1.0266271794171553 2.6134842275509804 5 0.5492608696467424 1.747816354871469 6 0.35537125854146034 1.357002616544298 7 0.25323072329671514 1.1281362898216747 8 0.167770377518763 0.8541859095226556 9 0.13049011689265883 0.747424387033752 >10 0.8302865992963466 9.818457950969613 >50 0.05201290159216762 2.2965932558765694 >100 0.03250748798039464 3.8518244593586743 >500 0.001422202598199949 0.6127696836404203 >1k 0.003250748795885598 3.7033839436896554 >5k 2.0317179974284986E-4 1.1718783800027703 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9063 1.16955819271449 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3562 0.45966747020291443 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3244 0.41863034063398497 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2447 0.3157794215571397 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2125 0.27422610167916706 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1975 0.2548689650900494 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1701 0.21950992892059445 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1644 0.21215421701672976 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1567 0.20221755356764934 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1462 0.18866755795526696 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1460 0.1884094628007454 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1422 0.18350565486483558 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1388 0.1791180372379689 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1372 0.17705327600179635 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1211 0.15627661606281004 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1059 0.1366613843191708 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1002 0.12930567241530608 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 3.871427317823535E-4 0.0 26-27 0.0 0.0 0.0 9.033330408254915E-4 0.0 28-29 0.0 0.0 0.0 0.0020002374475421598 0.0 30-31 0.0 0.0 0.0 0.004129522472345104 0.0 32-33 0.0 0.0 0.0 0.008000949790168639 0.0 34-35 0.0 0.0 0.0 0.012969281514708842 0.0 36-37 0.0 0.0 0.0 0.02297046875241964 0.0 38-39 0.0 0.0 0.0 0.03864974938960496 0.0 40-41 0.0 0.0 0.0 0.053296649408704 0.0 42-43 0.0 0.0 0.0 0.07755759393373149 0.0 44-45 0.0 0.0 0.0 0.10910972657399329 0.0 46-47 0.0 0.0 0.0 0.14524304820701295 0.0 48-49 0.0 0.0 0.0 0.1891837482643101 0.0 50-51 0.0 0.0 0.0 0.2388670655097121 0.0 52-53 0.0 0.0 0.0 0.29313157174787197 0.0 54-55 0.0 0.0 0.0 0.35133202909248584 0.0 56-57 0.0 0.0 0.0 0.43192224109184574 0.0 58-59 0.0 0.0 0.0 0.5272238768989351 0.0 60-61 0.0 0.0 0.0 0.6341397946594951 0.0 62-63 0.0 0.0 0.0 0.75008904282831 0.0 64-65 0.0 0.0 0.0 0.8651994817449298 0.0 66-67 0.0 0.0 0.0 1.0165077660831994 0.0 68-69 0.0 0.0 0.0 1.1923350901010186 0.0 70-71 0.0 0.0 0.0 1.3767440780066795 0.0 72-73 0.0 0.0 0.0 1.5866399624213456 0.0 74-75 0.0 0.0 0.0 1.792341800575036 0.0 76-77 0.0 0.0 0.0 2.0116581581297392 0.0 78-79 0.0 0.0 0.0 2.272850454505567 0.0 80-81 0.0 0.0 0.0 2.591017256242031 0.0 82-83 0.0 0.0 0.0 2.9128619139304277 0.0 84-85 0.0 0.0 0.0 3.265548942584152 0.0 86-87 0.0 0.0 0.0 3.6500461990326594 0.0 88-89 0.0 0.0 0.0 4.082549154222178 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 80 1.9081199E-9 29.685322 94-95 ACTGCGT 85 6.408028E-4 27.939129 8 TACCTGG 590 0.0 25.764147 2 TAACGCA 95 0.0012271799 24.996553 4 GTACCTG 700 0.0 24.434664 1 ATACGTC 140 1.7804101E-5 23.746725 5 TAAGGTG 360 0.0 22.427464 5 GAGTACT 4080 0.0 21.772747 12-13 GGTGTGC 725 0.0 21.619104 8 TCGTACA 110 0.0028755993 21.592112 2 GACCCCG 110 0.002877754 21.589325 7 GTAAGGT 310 1.0913936E-11 21.448656 4 AGCACCG 135 3.797939E-4 21.1082 5 GTACTTT 4285 0.0 20.730442 14-15 GTACATG 3485 0.0 20.449862 1 TAATACG 170 7.946183E-5 19.559914 3 GGTAAGG 340 4.1836756E-11 19.559914 3 GTATTAG 195 1.0297159E-5 19.492039 1 TAGACAG 390 0.0 19.484493 5 CTACACT 320 3.965397E-10 19.294214 4 >>END_MODULE