FastQCFastQC Report
Thu 26 May 2016
SRR936396_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936396_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences812958
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT53650.6599356916347462No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT46110.5671879728104035No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40780.5016249301931958No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30720.3778793000376403No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC30290.3725899738977905No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT28760.3537698134466971No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20440.2514275030198362No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT17600.21649334897989808No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT14950.18389633904826572No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC13190.1622470041502759No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC11400.14022864649834307No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC10410.12805089561822383No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG9990.12288457706302171No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA9980.12276156947837404No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA8870.10910772758248273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG6850.028.4483832
GTACCTG7100.027.444991
GTACATG39250.027.0024261
TACATGG40550.025.4350812
ACATGGG39800.024.4813333
CATGGGG18100.022.8457264
AGTACTT34950.022.43887312-13
ACCGTGC1100.002862168721.6096928
GAGTACT34400.021.07054112-13
GTGTAAG2509.416908E-920.9117431
GTGTAGC4100.020.8653761
TAGTACT2751.2478267E-920.7401964
GTACTTT35850.020.34776314-15
ATGGGGA9300.019.4242925
TACTTTT40500.019.0675914-15
GTCTATA1759.8894845E-519.0106741
GTATAGC2552.6566886E-718.6379151
CATGGGA20950.018.6034774
GTACTAA1851.5141169E-417.983071
AGAGTAC65600.017.89936610-11
Produced by FastQC (version 0.11.5)