##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936396_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 812958 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.787885721033557 33.0 31.0 34.0 30.0 34.0 2 31.86910270887303 33.0 31.0 34.0 30.0 34.0 3 31.846151953729468 33.0 31.0 34.0 30.0 34.0 4 35.501512993291165 37.0 35.0 37.0 33.0 37.0 5 35.53927755185385 37.0 35.0 37.0 33.0 37.0 6 35.59837900604951 37.0 35.0 37.0 33.0 37.0 7 35.49070431682818 37.0 35.0 37.0 33.0 37.0 8 35.49171543917398 37.0 35.0 37.0 33.0 37.0 9 37.06340671965833 39.0 37.0 39.0 33.0 39.0 10-11 36.9533106261332 39.0 37.0 39.0 33.0 39.0 12-13 36.881870281121536 39.0 37.0 39.0 33.0 39.0 14-15 37.95226014136031 40.0 38.0 41.0 33.0 41.0 16-17 37.90423675023802 40.0 38.0 41.0 33.0 41.0 18-19 37.81820278538375 40.0 38.0 41.0 33.0 41.0 20-21 37.79712543575437 40.0 37.5 41.0 33.0 41.0 22-23 37.76912890948856 40.0 37.5 41.0 32.5 41.0 24-25 37.88055717515542 40.0 38.0 41.0 33.0 41.0 26-27 37.79515915951377 40.0 37.5 41.0 33.0 41.0 28-29 37.689270540421525 40.0 37.0 41.0 33.0 41.0 30-31 37.49774896120095 40.0 37.0 41.0 32.0 41.0 32-33 37.31951773646363 39.5 36.5 41.0 32.0 41.0 34-35 37.11082122323663 39.0 36.0 41.0 31.0 41.0 36-37 36.890201584829725 39.0 36.0 41.0 31.0 41.0 38-39 36.70410771528172 39.0 35.5 41.0 30.5 41.0 40-41 36.51157316860157 39.0 35.0 40.0 30.0 41.0 42-43 36.24252913434643 39.0 35.0 40.0 30.0 41.0 44-45 36.071236767459084 39.0 35.0 40.0 30.0 41.0 46-47 35.88597012391784 39.0 35.0 40.0 29.0 41.0 48-49 35.637582753352575 39.0 35.0 40.0 28.0 41.0 50-51 34.63665404608848 38.0 34.0 39.5 25.5 40.5 52-53 34.74793654776754 38.0 34.5 39.5 26.5 40.5 54-55 35.19239370299573 39.0 35.0 40.0 27.0 41.0 56-57 35.024354886722314 38.0 35.0 40.0 26.0 41.0 58-59 34.80876256829996 38.0 34.0 40.0 26.0 41.0 60-61 34.5064314515633 38.0 34.0 40.0 26.0 41.0 62-63 34.20218695184745 37.5 34.0 40.0 25.0 41.0 64-65 33.85778724116129 37.0 33.5 40.0 23.5 41.0 66-67 33.469443070859754 36.0 33.0 39.0 23.0 41.0 68-69 33.02587157024102 36.0 33.0 39.0 22.0 41.0 70-71 32.60799635405519 35.0 32.0 38.5 21.0 40.0 72-73 32.19547504299115 35.0 32.0 38.0 20.0 40.0 74-75 31.791759844912036 35.0 31.5 37.0 18.5 39.0 76-77 31.33296000531393 35.0 31.0 36.5 17.5 39.0 78-79 30.861810819255115 35.0 31.0 36.0 12.0 39.0 80-81 30.390141311113243 34.0 30.0 36.0 7.5 37.5 82-83 29.98195909259765 34.0 30.0 35.0 7.0 37.0 84-85 29.61944651507212 34.0 29.5 35.0 3.5 36.5 86-87 29.3154437252601 34.0 29.0 35.0 2.0 36.0 88-89 28.956055540384618 34.0 29.0 35.0 2.0 36.0 90-91 28.5958696513227 34.0 29.0 35.0 2.0 35.5 92-93 28.170136095591655 34.0 29.0 35.0 2.0 35.0 94-95 27.698060662420446 34.0 27.0 35.0 2.0 35.0 96-97 27.12425918682146 33.5 26.5 35.0 2.0 35.0 98-99 26.447441934269666 33.0 25.0 35.0 2.0 35.0 100-101 23.914966086808914 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4126298412348035 1101 2 0.36832443027890704 1101 3 0.4875026016340094 1101 4 0.48895540224543765 1101 5 0.3690305847160147 1101 6 0.44276737324628357 1101 7 0.35208017803785907 1101 8 0.23106546179036513 1101 9 0.5045788404974587 1101 10-11 0.49622164739803054 1101 12-13 0.46952973224615135 1101 14-15 0.798068408434041 1101 16-17 0.7574565292110336 1101 18-19 0.8467248910702878 1101 20-21 0.7479725252688922 1101 22-23 0.5995433796288197 1101 24-25 0.4664913549513088 1101 26-27 0.5187813134903578 1101 28-29 0.4447519460906264 1101 30-31 0.4691165326909399 1101 32-33 0.421659812839998 1101 34-35 0.4625401309376471 1101 36-37 0.39856743785720994 1101 38-39 0.6123927000493978 1101 40-41 0.4374591211762251 1101 42-43 0.5756585493669135 1101 44-45 0.4417491083027798 1101 46-47 0.6692807415680022 1101 48-49 0.8834869534320191 1101 50-51 0.7839140985473279 1101 52-53 0.8582859941756524 1101 54-55 0.7404072087676568 1101 56-57 0.9163067135986367 1101 58-59 0.6957254142954383 1101 60-61 0.5429643052311377 1101 62-63 0.7030406815411254 1101 64-65 0.5638690230751848 1101 66-67 0.6541156384519056 1101 68-69 0.7615242368488424 1101 70-71 0.6402892439367847 1101 72-73 0.7829814590644375 1101 74-75 0.4381719867921916 1101 76-77 0.4576603450973167 1101 78-79 0.4886631424891199 1101 80-81 0.7112246675459843 1101 82-83 0.5635505458813377 1101 84-85 0.6257267765996737 1101 86-87 0.5962782943516807 1101 88-89 0.5685789962791112 1101 90-91 0.6546619277161696 1101 92-93 0.9222099865429065 1101 94-95 0.9285461109016815 1101 96-97 0.8025607619994766 1101 98-99 0.742299958609486 1101 100-101 1.229463332853829 1102 1 0.41272333412933904 1102 2 0.42154705843608653 1102 3 0.47117730389510015 1102 4 0.3981738016470828 1102 5 0.3955178409153106 1102 6 0.44769013373187505 1102 7 0.45656208356688666 1102 8 0.3611140780955253 1102 9 0.41528593445062256 1102 10-11 0.3813152841277869 1102 12-13 0.42456324585295846 1102 14-15 0.6304949805530242 1102 16-17 0.5045150989135792 1102 18-19 0.5118009799029153 1102 20-21 0.4625405161785068 1102 22-23 0.28228782758923643 1102 24-25 0.4309137126982705 1102 26-27 0.23572797938989964 1102 28-29 0.26857177809107924 1102 30-31 0.2191363100340169 1102 32-33 0.3755372508470174 1102 34-35 -0.05177327162837031 1102 36-37 0.12229054067062606 1102 38-39 0.018085338562713105 1102 40-41 0.24933343795892426 1102 42-43 0.2069495424052903 1102 44-45 0.22404011572468363 1102 46-47 0.3469711794024164 1102 48-49 0.40602773071756104 1102 50-51 0.47595929157419903 1102 52-53 0.4229129575264423 1102 54-55 0.4636538574069746 1102 56-57 0.49420966797409704 1102 58-59 0.2189979532747799 1102 60-61 0.390867547277054 1102 62-63 0.33047149679917 1102 64-65 0.5362436706788927 1102 66-67 0.2380578742265982 1102 68-69 0.4206185783711973 1102 70-71 0.1899159915347397 1102 72-73 0.345055347218171 1102 74-75 0.07575936668978045 1102 76-77 0.00663264243348749 1102 78-79 0.17653172024644093 1102 80-81 0.28469030531135786 1102 82-83 0.2491555168266224 1102 84-85 0.24818089316373815 1102 86-87 0.0323309524265909 1102 88-89 -0.024514755518829645 1102 90-91 0.14514722775644273 1102 92-93 0.2623299451183634 1102 94-95 0.27782657520178233 1102 96-97 0.2663106900818697 1102 98-99 0.4227070842064009 1102 100-101 0.8855101656022342 1103 1 0.3799245003100964 1103 2 0.44356713020105687 1103 3 0.3795087683490159 1103 4 0.44445660048089763 1103 5 0.3961626043151796 1103 6 0.448012515431806 1103 7 0.5020599530443519 1103 8 0.306813786550336 1103 9 0.3665642479738338 1103 10-11 0.3418305341838632 1103 12-13 0.41466752932458917 1103 14-15 0.700907348797017 1103 16-17 0.8187811899653852 1103 18-19 0.7346087843433722 1103 20-21 0.6459126287598025 1103 22-23 0.5108634611393583 1103 24-25 0.39908202397883485 1103 26-27 0.392489585131095 1103 28-29 0.39218413512180206 1103 30-31 0.4135254667956261 1103 32-33 0.5995014244285386 1103 34-35 0.5537787019082927 1103 36-37 0.49455029622119184 1103 38-39 0.36323699812763977 1103 40-41 0.5081779098135968 1103 42-43 0.6020283156728325 1103 44-45 0.7590115218695601 1103 46-47 0.9476243527596679 1103 48-49 0.9207381478514449 1103 50-51 0.9101934247879271 1103 52-53 1.0357956105876625 1103 54-55 0.9678868692930536 1103 56-57 0.9427337204522317 1103 58-59 0.8683447799175283 1103 60-61 1.0608958608002652 1103 62-63 1.195996278140278 1103 64-65 0.9907528094992486 1103 66-67 0.9081072126432872 1103 68-69 0.9920681746932374 1103 70-71 1.01290040508038 1103 72-73 1.0638894467920608 1103 74-75 0.9191309186072516 1103 76-77 0.9544348402356846 1103 78-79 0.7229897265258778 1103 80-81 1.03363205233984 1103 82-83 0.8789211593473674 1103 84-85 0.9777446967071306 1103 86-87 0.7485018988271293 1103 88-89 0.5603697477330201 1103 90-91 0.7692641822305397 1103 92-93 0.7298254598077527 1103 94-95 0.9376788402836347 1103 96-97 0.9288191000392558 1103 98-99 1.0607214932667262 1103 100-101 1.4236633389594857 1104 1 0.11445017871333008 1104 2 0.1559465874784749 1104 3 0.42565140122634304 1104 4 0.35873110149009335 1104 5 0.334882048136663 1104 6 0.31788244142418165 1104 7 0.22049457964224217 1104 8 0.2757530105916004 1104 9 0.3371854634930074 1104 10-11 0.23011826685934267 1104 12-13 0.5063921312532713 1104 14-15 0.5210463934258627 1104 16-17 0.37333378023225805 1104 18-19 0.4254587507035481 1104 20-21 0.5535432634312514 1104 22-23 0.3845739343396346 1104 24-25 0.43061936418963853 1104 26-27 0.09208590717640419 1104 28-29 0.11443127573629397 1104 30-31 0.05248599606227344 1104 32-33 -0.05229830645283329 1104 34-35 0.04021063731982366 1104 36-37 0.14834038368475433 1104 38-39 -0.028667016225355724 1104 40-41 0.09480047092595356 1104 42-43 0.26596641838644786 1104 44-45 0.32912253330710683 1104 46-47 0.48031105381372186 1104 48-49 0.5377486883313765 1104 50-51 0.37489963694311257 1104 52-53 0.30895102660022644 1104 54-55 0.4701295245969348 1104 56-57 0.6165114733116255 1104 58-59 0.5862467758807455 1104 60-61 0.39376197427861825 1104 62-63 0.39876035080230565 1104 64-65 0.38509571149521804 1104 66-67 0.3504990045248775 1104 68-69 0.25308521887355084 1104 70-71 0.2263170904358347 1104 72-73 0.5798963990235109 1104 74-75 0.5769858188090922 1104 76-77 0.3094191416485579 1104 78-79 0.242416728095737 1104 80-81 0.4349552189691295 1104 82-83 0.30861391557073503 1104 84-85 0.27702704700989145 1104 86-87 0.11391454740304496 1104 88-89 0.18604254432418088 1104 90-91 0.3563913407862671 1104 92-93 0.3307169152910454 1104 94-95 0.4587037337575097 1104 96-97 0.361483374540267 1104 98-99 0.16323887384533364 1104 100-101 0.7081161310653385 1105 1 0.09213088310210793 1105 2 0.027644765077273092 1105 3 0.3124338734125409 1105 4 0.3142631470842545 1105 5 0.3026487534810087 1105 6 0.2140840658866736 1105 7 0.26825582460845254 1105 8 0.2560615219560134 1105 9 0.2939290304850246 1105 10-11 0.12650759182010063 1105 12-13 0.16273811446261277 1105 14-15 0.3521415405148005 1105 16-17 0.2536491170693367 1105 18-19 0.1428329913697084 1105 20-21 0.31990733414314576 1105 22-23 0.25990667613199747 1105 24-25 0.23785948431795845 1105 26-27 0.16071603481273655 1105 28-29 0.24835558124444646 1105 30-31 -0.08433060348724553 1105 32-33 -0.013811172468798816 1105 34-35 0.08569388078442586 1105 36-37 -0.18792505894715106 1105 38-39 -0.23449767624856577 1105 40-41 0.012681502937425648 1105 42-43 -0.1515221489468388 1105 44-45 -0.07526112651134298 1105 46-47 0.019361289292525896 1105 48-49 -0.044745680653676345 1105 50-51 -0.02081567259207162 1105 52-53 0.1569221548508537 1105 54-55 0.040338042786821404 1105 56-57 -0.059287106990062455 1105 58-59 -0.06268503764734135 1105 60-61 -0.09732225205404887 1105 62-63 -0.11111952087880894 1105 64-65 0.06644852017721803 1105 66-67 0.11747736777653017 1105 68-69 0.008724765662165623 1105 70-71 0.18080825144873813 1105 72-73 0.28567586800173217 1105 74-75 0.04209058981448521 1105 76-77 -0.0031021879535160224 1105 78-79 0.05934048756607879 1105 80-81 -0.01477517486064528 1105 82-83 -0.08105231881599906 1105 84-85 0.008035169322358371 1105 86-87 0.024074263463379708 1105 88-89 0.1317203137597147 1105 90-91 0.17545659230493982 1105 92-93 0.18764647641315335 1105 94-95 0.2349306124688617 1105 96-97 0.42206797627393655 1105 98-99 0.5926037636139512 1105 100-101 0.8329540260895705 1106 1 0.1007767238478543 1106 2 0.04317525678722589 1106 3 0.08449322017123961 1106 4 0.1853582869120487 1106 5 0.2328238050714191 1106 6 0.10288005250875187 1106 7 0.13598869303512373 1106 8 0.34960026813121203 1106 9 0.46921188823781534 1106 10-11 0.3059176821922662 1106 12-13 0.3783059647436957 1106 14-15 0.28075998497505594 1106 16-17 0.1670839708372185 1106 18-19 0.25035451702259337 1106 20-21 0.003782663254376928 1106 22-23 0.03449217389049153 1106 24-25 -0.12616421169001057 1106 26-27 -0.22199584165492325 1106 28-29 -0.06747555801407401 1106 30-31 -0.08336995451549711 1106 32-33 0.0869769154839517 1106 34-35 -0.17237379159869448 1106 36-37 -0.247405241114663 1106 38-39 -0.03794711764896874 1106 40-41 0.009932979490471894 1106 42-43 -0.09972715855332837 1106 44-45 -0.042145421679634865 1106 46-47 0.06925277153830933 1106 48-49 0.15997039420577863 1106 50-51 0.11923449463200342 1106 52-53 0.21965786964485545 1106 54-55 0.23443360956971304 1106 56-57 0.2981899441733873 1106 58-59 0.09710290798704335 1106 60-61 -0.10281040405006081 1106 62-63 0.028370267778420555 1106 64-65 -0.04151775036999794 1106 66-67 -0.23557891913729634 1106 68-69 0.08553220891271707 1106 70-71 -0.13903448675662844 1106 72-73 -0.1088217481656315 1106 74-75 0.08247342621952214 1106 76-77 -0.226630971843079 1106 78-79 -0.0897380010128721 1106 80-81 0.1053593159954005 1106 82-83 0.18469165044323432 1106 84-85 0.391209580480119 1106 86-87 0.36992568308908247 1106 88-89 0.4217384593514062 1106 90-91 0.449926456390898 1106 92-93 0.643590047180961 1106 94-95 0.5434590405625066 1106 96-97 0.41118951615866095 1106 98-99 0.8799712296354407 1106 100-101 1.4338043783275296 1107 1 0.08831584427730377 1107 2 0.2065216507548584 1107 3 0.2687770460411336 1107 4 0.18302060012815957 1107 5 0.2738719570100372 1107 6 0.26176898567736373 1107 7 0.3001134680211166 1107 8 0.16334335660803134 1107 9 0.11858515585250728 1107 10-11 0.4110207413653342 1107 12-13 0.33486204421317467 1107 14-15 0.2056292585115429 1107 16-17 0.22214170924183918 1107 18-19 0.4357970113691536 1107 20-21 0.2213800096364693 1107 22-23 0.30335964646696567 1107 24-25 0.20624899840248645 1107 26-27 -0.009568200140925853 1107 28-29 0.1591003031865199 1107 30-31 0.08250109030415587 1107 32-33 0.2879459065817471 1107 34-35 0.19552952296475468 1107 36-37 0.13171549358206391 1107 38-39 0.32007508232032933 1107 40-41 0.2863476954704254 1107 42-43 0.3528734226039134 1107 44-45 0.3999215784416208 1107 46-47 0.48977481252455846 1107 48-49 0.3615532718583623 1107 50-51 0.42916156592679044 1107 52-53 0.4688284020727309 1107 54-55 0.32934945878086097 1107 56-57 0.40437154436535394 1107 58-59 0.6114012076739144 1107 60-61 0.5458616423204177 1107 62-63 0.39358528493587386 1107 64-65 0.4579743517759809 1107 66-67 0.5272836576091251 1107 68-69 0.4791988329012682 1107 70-71 0.4670932711753579 1107 72-73 0.33935656044124585 1107 74-75 0.32907224881224195 1107 76-77 0.5059112053004995 1107 78-79 0.4457936510287688 1107 80-81 0.5540756232473178 1107 82-83 0.5879451643926181 1107 84-85 0.44409073492600726 1107 86-87 0.5301386271075472 1107 88-89 0.588509136263081 1107 90-91 0.7050560975301146 1107 92-93 0.6120626482539038 1107 94-95 0.5108403819724607 1107 96-97 0.7009923008633869 1107 98-99 0.6900225196875098 1107 100-101 1.1088837214689136 1108 1 0.056901699116956195 1108 2 0.08623215657494399 1108 3 0.20662162835676767 1108 4 0.3181779304662342 1108 5 0.26204423359777707 1108 6 0.27523491553966295 1108 7 0.25587447637639116 1108 8 0.29949145216654216 1108 9 -0.06797964390165845 1108 10-11 0.10878860560400483 1108 12-13 -0.037437325829692725 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-2.887768823650074 2316 72-73 -2.2389319155190925 2316 74-75 -2.6717768470964316 2316 76-77 -2.917188536387691 2316 78-79 -3.3150529450882225 2316 80-81 -3.820600653729599 2316 82-83 -3.3185317958606895 2316 84-85 -3.184001924019082 2316 86-87 -3.7117512154055774 2316 88-89 -5.263580689584764 2316 90-91 -4.239975149865934 2316 92-93 -3.65604917576076 2316 94-95 -3.2688729752477705 2316 96-97 -4.912235763464587 2316 98-99 -7.200705752956438 2316 100-101 -7.788675648583585 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1738.0 3 593.0 4 552.0 5 614.0 6 614.0 7 753.0 8 912.0 9 1104.0 10 1405.0 11 1819.0 12 2566.0 13 3278.0 14 3781.0 15 4710.0 16 6241.0 17 8680.0 18 6721.0 19 4622.0 20 4339.0 21 4681.0 22 4986.0 23 5591.0 24 6419.0 25 7353.0 26 8767.0 27 10239.0 28 12455.0 29 15502.0 30 18948.0 31 24230.0 32 31611.0 33 42041.0 34 58070.0 35 82745.0 36 118464.0 37 158342.0 38 127149.0 39 20315.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.54102493402135 17.80881947453421 14.148727695277111 22.501427896167332 2 15.258923309188402 19.843343000328524 40.2771642769871 24.62056941349598 3 20.465153472350373 24.082303007935042 29.68591991142277 25.766623608291813 4 12.231625802308285 15.762212863753074 38.00034694412354 34.0058143898151 5 13.204703415845302 37.39651144049185 34.26032670949136 15.138458434171495 6 31.349626747678478 35.75372114169561 17.582294538962326 15.314357571663583 7 28.471294690284005 32.69575215334447 20.563654918316324 18.2692982380552 8 26.110341194686015 33.11023534827163 19.798202580190967 20.98122087685139 9 25.84589554948592 17.374200385759636 21.27340067920283 35.50650338555161 10-11 25.19043100360739 25.75504708313487 27.057633301702527 21.996888611555214 12-13 27.006076371798155 23.370589769841114 27.481677364099937 22.141656494260793 14-15 23.1084332753564 24.687715144777247 27.219648090052722 24.984203489813638 16-17 21.75100031933922 28.34354008753217 27.483969971037965 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21.421779493910368 26.528435712888665 29.071307933313566 22.978476859887394 44-45 21.82151085481766 26.186809388442438 29.035176522251483 22.95650323448842 46-47 22.43914019788315 26.68285863476984 28.708494755856574 22.169506411490435 48-49 22.468798972635597 26.48021905298455 28.655311370631058 22.395670603748798 50-51 21.598402851839417 26.864108326598597 29.256939857235814 22.280548964326176 52-53 22.193534102336223 27.309422694298284 28.142799592844643 22.354243610520847 54-55 21.43679097615804 26.547205727236662 28.747259235568894 23.2687440610364 56-57 22.284001948521635 26.688411216792712 28.20337448519173 22.824212349493926 58-59 22.983137121520116 26.39550667669611 28.12675482000813 22.49460138177564 60-61 22.239669634982086 26.40201545295411 29.42248858742384 21.93582632463997 62-63 21.893601396407867 26.781213443008923 29.09988769200301 22.22529746858021 64-65 21.53800924653132 27.45944263724801 28.632141936777444 22.37040617944323 66-67 21.732950614087258 27.09186061458544 27.890244779996916 23.284943991330387 68-69 21.385113483578678 26.95528681804371 28.5509209200713 23.108678778306313 70-71 22.186337756942784 26.856375280844976 28.07219645524901 22.88509050696323 72-73 21.978291693450906 26.34732675338097 29.02195743843213 22.652424114735997 74-75 21.447125696531966 27.71488307120542 28.25488564235333 22.58310558990929 76-77 21.77972607863305 26.829505356870424 28.09244230967977 23.298326254816754 78-79 21.966719589380435 26.813484650054964 28.378467324581504 22.8413284359831 80-81 22.279631481761694 27.086078213555066 27.911295312821004 22.72299499186224 82-83 21.93840521769509 26.77549986099596 28.582060359737543 22.704034561571408 84-85 21.82292781455813 27.110318918879024 28.116550667089825 22.95020259947302 86-87 21.81897913833622 27.332844988717024 28.109460539110316 22.73871533383644 88-89 21.65791451553184 27.24286942626974 27.524254468025433 23.57496159017299 90-91 21.772598804899726 26.886434720030998 27.87326893446631 23.467697540602963 92-93 22.268552719068445 27.08593340095303 28.02309567408208 22.622418205896448 94-95 21.91004773858374 26.86640885412021 28.05898153997376 23.164561867322288 96-97 21.251916830029078 26.95132220521107 28.55232507714991 23.244435887609942 98-99 21.665734676137905 27.443952919406506 27.390309636166894 23.5000027682887 100-101 21.50724698306382 26.850778156379317 27.84790347772755 23.794071382829316 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 83.0 1 90.0 2 246.0 3 345.0 4 233.0 5 183.5 6 352.5 7 742.5 8 1193.5 9 1405.0 10 1213.5 11 1008.0 12 998.5 13 1050.5 14 1176.0 15 1233.0 16 1245.0 17 1345.0 18 1408.0 19 1508.0 20 1688.0 21 1969.0 22 2287.5 23 2626.0 24 3161.5 25 3883.5 26 4795.0 27 5807.0 28 6691.0 29 7715.5 30 9242.5 31 10924.5 32 12698.5 33 13870.0 34 15238.0 35 16609.5 36 17833.5 37 19405.0 38 20529.5 39 21117.5 40 21493.0 41 22819.0 42 24161.5 43 25369.0 44 26612.0 45 27667.5 46 28696.0 47 33410.5 48 44949.0 49 44511.0 50 33811.5 51 29180.0 52 26747.5 53 25949.5 54 25330.0 55 23395.0 56 21091.0 57 18727.5 58 16746.5 59 14886.0 60 12653.5 61 10603.5 62 8939.0 63 7152.0 64 5332.0 65 4125.0 66 3141.0 67 2527.5 68 1968.0 69 1329.0 70 980.5 71 756.0 72 625.5 73 533.0 74 436.5 75 380.5 76 277.5 77 171.5 78 108.0 79 76.5 80 55.5 81 37.5 82 30.0 83 17.5 84 6.5 85 3.5 86 4.5 87 6.0 88 5.0 89 2.5 90 2.5 91 1.5 92 1.0 93 1.5 94 0.5 95 0.5 96 0.5 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07060635358776221 2 0.02792272171502095 3 0.013284819141948293 4 0.018082114943207397 5 0.023002418329114174 6 0.02915279756149764 7 0.075895679727612 8 0.05658348893792791 9 0.06580905778650312 10-11 0.056460481353280245 12-13 0.037148290563596156 14-15 0.03425761232437592 16-17 0.042130097741826765 18-19 0.03868588537169202 20-21 0.016483016342787694 22-23 0.012731285011033782 24-25 0.020357755259189283 26-27 0.022079861444256655 28-29 0.006396394401678808 30-31 3.690227539430081E-4 32-33 0.009164065056251369 34-35 0.01008662194110889 36-37 0.00405925029337309 38-39 0.007318951286536327 40-41 0.004428273047316098 42-43 0.002337144108305718 44-45 1.2300758464766937E-4 46-47 4.3052654626684277E-4 48-49 4.920303385906775E-4 50-51 0.0011070682618290245 52-53 6.150379232383469E-5 54-55 6.150379232383469E-5 56-57 0.0041822578780207585 58-59 0.0022756403159818832 60-61 0.004797295801259106 62-63 0.0018451137697150408 64-65 5.535341309145123E-4 66-67 4.3052654626684277E-4 68-69 0.002583159277601057 70-71 0.001168572054152859 72-73 0.0166675277197592 74-75 0.011255193995261748 76-77 0.020911289390103796 78-79 0.01691354288905454 80-81 0.009963614356461219 82-83 0.00393624270872542 84-85 0.002952182031544065 86-87 9.225568848575204E-4 88-89 0.0018451137697150408 90-91 0.006826920947945651 92-93 0.009656095394842046 94-95 0.011501209164557087 96-97 0.011378201579909417 98-99 0.022325876613551992 100-101 0.021095800767075297 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 812958.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.20824393347414 #Duplication Level Percentage of deduplicated Percentage of total 1 85.26793734773244 54.74904520927395 2 9.003230295317646 11.561632139819997 3 2.3534142943969343 4.533257972734898 4 1.0212678255197365 2.622952546495198 5 0.5435963354840109 1.745168305505001 6 0.35442783163461267 1.3654313202244506 7 0.2538813457094784 1.1410892762831006 8 0.1806965783897491 0.9281767986554513 9 0.12718831958745158 0.7349884784603783 >10 0.803633076346499 9.59143875829611 >50 0.05505072706804933 2.3806779938361733 >100 0.030305438751425596 3.313132838294899 >500 0.003068905178262692 1.412964573784915 >1k 0.002109872310055601 3.2593138189852504 >5k 1.9180657364141826E-4 0.6607299693503067 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5365 0.6599356916347462 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4611 0.5671879728104035 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4078 0.5016249301931958 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3072 0.3778793000376403 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3029 0.3725899738977905 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2876 0.3537698134466971 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2044 0.2514275030198362 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1760 0.21649334897989808 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1495 0.18389633904826572 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1319 0.1622470041502759 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1140 0.14022864649834307 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1041 0.12805089561822383 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 999 0.12288457706302171 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 998 0.12276156947837404 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 887 0.10910772758248273 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 3.690227539430081E-4 0.0 28-29 0.0 0.0 0.0 0.0016606023927435366 6.150379232383469E-5 30-31 0.0 0.0 0.0 0.003567219954782412 1.2300758464766937E-4 32-33 0.0 0.0 0.0 0.007564966455831667 1.2300758464766937E-4 34-35 0.0 0.0 0.0 0.012054743295471598 1.2300758464766937E-4 36-37 0.0 0.0 0.0 0.01992722871292244 1.2300758464766937E-4 38-39 0.0 0.0 0.0 0.03370407819346141 1.2300758464766937E-4 40-41 0.0 0.0 0.0 0.04908002627442008 1.2300758464766937E-4 42-43 0.0 0.0 0.0 0.07343552803465861 1.2300758464766937E-4 44-45 0.0 0.0 0.0 0.10111223458038424 1.2300758464766937E-4 46-47 0.0 0.0 0.0 0.13709195308982752 1.2300758464766937E-4 48-49 0.0 0.0 0.0 0.1780534787775014 1.2300758464766937E-4 50-51 0.0 0.0 0.0 0.22442733818967275 1.2300758464766937E-4 52-53 0.0 0.0 0.0 0.27664405787260843 1.2300758464766937E-4 54-55 0.0 0.0 0.0 0.331136417871526 1.2300758464766937E-4 56-57 0.0 0.0 0.0 0.409984279630682 1.2300758464766937E-4 58-59 0.0 0.0 0.0 0.4923378575522967 1.2300758464766937E-4 60-61 0.0 0.0 0.0 0.5830559512299529 1.2300758464766937E-4 62-63 0.0 0.0 0.0 0.6891499929885677 1.2300758464766937E-4 64-65 0.0 0.0 0.0 0.7973966674785167 1.2300758464766937E-4 66-67 0.0 0.0 0.0 0.935657192622497 1.2300758464766937E-4 68-69 0.0 0.0 0.0 1.100487356050374 1.2300758464766937E-4 70-71 0.0 0.0 0.0 1.2792173765434378 1.2300758464766937E-4 72-73 0.0 0.0 0.0 1.4802117698577295 1.2300758464766937E-4 74-75 0.0 0.0 0.0 1.6754863104859046 1.2300758464766937E-4 76-77 0.0 0.0 0.0 1.8828770982018752 1.2300758464766937E-4 78-79 0.0 0.0 0.0 2.1504800986028796 1.2300758464766937E-4 80-81 0.0 0.0 0.0 2.4685777125017525 1.2300758464766937E-4 82-83 0.0 0.0 0.0 2.7663790749337602 1.2300758464766937E-4 84-85 0.0 0.0 0.0 3.08121698783947 1.2300758464766937E-4 86-87 0.0 0.0 0.0 3.446365002866077 1.2300758464766937E-4 88-89 0.0 0.0 0.0 3.8661898892685724 1.2300758464766937E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 685 0.0 28.448383 2 GTACCTG 710 0.0 27.44499 1 GTACATG 3925 0.0 27.002426 1 TACATGG 4055 0.0 25.435081 2 ACATGGG 3980 0.0 24.481333 3 CATGGGG 1810 0.0 22.845726 4 AGTACTT 3495 0.0 22.438873 12-13 ACCGTGC 110 0.0028621687 21.609692 8 GAGTACT 3440 0.0 21.070541 12-13 GTGTAAG 250 9.416908E-9 20.911743 1 GTGTAGC 410 0.0 20.865376 1 TAGTACT 275 1.2478267E-9 20.740196 4 GTACTTT 3585 0.0 20.347763 14-15 ATGGGGA 930 0.0 19.424292 5 TACTTTT 4050 0.0 19.06759 14-15 GTCTATA 175 9.8894845E-5 19.010674 1 GTATAGC 255 2.6566886E-7 18.637915 1 CATGGGA 2095 0.0 18.603477 4 GTACTAA 185 1.5141169E-4 17.98307 1 AGAGTAC 6560 0.0 17.899366 10-11 >>END_MODULE