FastQCFastQC Report
Thu 26 May 2016
SRR936396_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936396_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences812958
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT94231.1591004701349885No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38000.4674288216611437No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32720.40248081696717425No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26200.32227987177689377No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22110.271969769655997No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT19870.24441607069491905No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC18040.22190568270439556No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC17240.212065075932582No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT16580.2039465753458358No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG15580.1916458168810689No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA15110.18586446040262844No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT14450.17774595981588226No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC14140.17393272469180449No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA13500.16606023927435368No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12400.15252940496311002No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA10640.1308800700651202No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10380.12768187286428082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG652.5867648E-643.850272
ATAACGC851.63263E-533.5263753
TAACGCA1252.1566302E-730.3935074
TACCTGG6800.026.546612
GTACCTG7200.025.7363341
CCGTAAC652.9464895E-421.91839246-47
GTATAGG2405.9026206E-921.77691
GAGTACT38800.021.23710412-13
GTACATG37500.020.5257151
GTACTTT41350.019.92743914-15
TACATGG38250.019.8711452
ACATGGG37050.019.74183
AGTACTT40150.019.10356912-13
GTACACG1759.9110075E-519.0052911
CCGTGTA1250.00603710218.9959419
GGTATGC1250.00603710218.9959418
GTCCGCG1250.00603710218.9959418
TATAAGG2653.998466E-717.926212
CTACGAC1600.001191331517.8108863
AGTACAT29800.017.6946142
Produced by FastQC (version 0.11.5)