FastQCFastQC Report
Thu 26 May 2016
SRR936395_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936395_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1518563
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75020.49401967517975875No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59670.39293727030093584No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37670.2480634652628834No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25110.16535369293206803No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16930.1114869781497376No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16170.10648224670296853No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16090.10595543286646653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG16950.038.9528962
GTACCTG19100.035.0666851
GTATCAA116600.028.8024751
GTACATG78800.028.3322221
GTATAGG4500.027.4454141
TACATGG80900.027.3610062
ACATGGG80500.026.4348473
CATGGGG31700.026.0726184
GTATACG950.001226483425.0008851
GTCCGTA950.001226718925.000062
ATCAACG134850.024.692313
TCAACGC135400.024.592014
TATCAAC135450.024.5829322
CAACGCA136700.024.219955
GAGTACT70050.024.07730512-13
AACGCAG139150.023.8641436
GGTATCA81900.023.1418441
GTACACG2955.456968E-1222.543171
GTACTTT75900.022.31580714-15
AGTACTT71700.021.96605512-13
Produced by FastQC (version 0.11.5)