##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936395_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1518563 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.612204432743322 31.0 31.0 34.0 30.0 34.0 2 31.697791267138737 33.0 31.0 34.0 30.0 34.0 3 31.71125004362677 33.0 31.0 34.0 30.0 34.0 4 35.35973285270351 37.0 35.0 37.0 33.0 37.0 5 35.37213207486288 37.0 35.0 37.0 33.0 37.0 6 35.37649804453289 37.0 35.0 37.0 33.0 37.0 7 35.30199405622289 37.0 35.0 37.0 33.0 37.0 8 35.26462188266144 37.0 35.0 37.0 33.0 37.0 9 36.803293640105814 39.0 37.0 39.0 33.0 39.0 10-11 36.678858894889444 39.0 37.0 39.0 32.0 39.0 12-13 36.58478706513988 39.0 37.0 39.0 32.0 39.0 14-15 37.71237940078878 40.0 37.0 41.0 32.5 41.0 16-17 37.6117717868801 40.0 37.0 41.0 32.0 41.0 18-19 37.592872011236935 40.0 37.0 41.0 32.0 41.0 20-21 37.463602432036076 40.0 37.0 41.0 32.0 41.0 22-23 37.55553836093728 40.0 37.0 41.0 32.0 41.0 24-25 37.58477619960449 40.0 37.0 41.0 32.0 41.0 26-27 37.48336717014704 40.0 37.0 41.0 32.0 41.0 28-29 37.33692576468675 40.0 37.0 41.0 31.5 41.0 30-31 37.105668319325574 39.0 36.5 41.0 31.0 41.0 32-33 36.87661130950774 39.0 36.0 41.0 31.0 41.0 34-35 36.66158993732891 39.0 35.0 41.0 30.0 41.0 36-37 36.43768549609071 39.0 35.0 41.0 30.0 41.0 38-39 36.22343393063047 39.0 35.0 40.0 30.0 41.0 40-41 35.99830629351565 39.0 35.0 40.0 29.5 41.0 42-43 35.74207853082157 38.0 35.0 40.0 28.5 41.0 44-45 35.43698417517087 38.0 35.0 40.0 27.5 41.0 46-47 35.17828763113549 38.0 35.0 40.0 26.0 41.0 48-49 34.97202256343662 38.0 34.5 40.0 25.0 41.0 50-51 33.997061695826915 37.5 33.0 39.5 23.5 40.5 52-53 34.09264844461507 38.0 33.5 39.5 24.0 40.5 54-55 34.51624990204555 38.0 34.0 40.0 24.0 41.0 56-57 34.403552239847805 38.0 34.0 40.0 23.5 41.0 58-59 34.167828730187686 38.0 34.0 40.0 23.0 41.0 60-61 33.87266152276857 37.5 33.5 40.0 22.0 41.0 62-63 33.50606066393031 37.0 33.0 40.0 22.0 41.0 64-65 33.15221495584971 36.5 33.0 39.5 20.5 41.0 66-67 32.709397305215525 36.0 32.0 39.0 18.5 41.0 68-69 32.33035903021475 35.0 32.0 39.0 18.0 40.5 70-71 31.926127859035155 35.0 31.5 38.0 15.0 40.0 72-73 31.540999945343064 35.0 31.0 37.5 12.5 40.0 74-75 31.113693998866033 35.0 31.0 37.0 8.0 39.0 76-77 30.66030187749866 35.0 30.5 36.5 7.0 39.0 78-79 30.1871338232263 34.0 30.0 36.0 4.0 38.5 80-81 29.73744454461224 34.0 29.5 35.5 2.0 37.0 82-83 29.338331040595612 34.0 29.0 35.0 2.0 37.0 84-85 28.978054252605915 34.0 29.0 35.0 2.0 36.5 86-87 28.621950159459963 34.0 29.0 35.0 2.0 36.0 88-89 28.220464017627194 34.0 28.5 35.0 2.0 36.0 90-91 27.826737514347446 34.0 27.0 35.0 2.0 35.0 92-93 27.38815972732116 33.5 26.5 35.0 2.0 35.0 94-95 26.901657685588283 33.0 25.0 35.0 2.0 35.0 96-97 26.330546378385357 33.0 25.0 35.0 2.0 35.0 98-99 25.588050018339707 33.0 22.0 35.0 2.0 35.0 100-101 22.738614400587927 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.41802328287134927 1101 2 0.2387927502634426 1101 3 0.3183311863784617 1101 4 0.312151467537781 1101 5 0.25811640436162975 1101 6 0.3123873885491051 1101 7 0.41570954450484265 1101 8 0.5681713474311252 1101 9 0.6193991680482114 1101 10-11 0.6732184261651142 1101 12-13 0.5821117403534473 1101 14-15 0.6249560336813502 1101 16-17 0.7236099640606781 1101 18-19 0.6409183185430152 1101 20-21 0.6843570580514893 1101 22-23 0.559693652829516 1101 24-25 0.5130757758674491 1101 26-27 0.5471682529304189 1101 28-29 0.5121516708255669 1101 30-31 0.35791305108050864 1101 32-33 0.3146805752606454 1101 34-35 0.25571387051626715 1101 36-37 0.17797979055810487 1101 38-39 0.26170664494478757 1101 40-41 0.26202961497536137 1101 42-43 0.31650894092209825 1101 44-45 0.12814807921318305 1101 46-47 0.25810537706159664 1101 48-49 0.27181240377046123 1101 50-51 0.1636890523571921 1101 52-53 0.2207273882364973 1101 54-55 0.20525568015916207 1101 56-57 0.21771596695867146 1101 58-59 0.17680562955317924 1101 60-61 0.32042318204983644 1101 62-63 0.29652884351563813 1101 64-65 0.21541709267630438 1101 66-67 0.264527785134824 1101 68-69 0.2404215462139021 1101 70-71 0.035344513208347195 1101 72-73 0.0334641903102586 1101 74-75 0.10645766500341125 1101 76-77 0.04945502264557433 1101 78-79 -0.11085771700715341 1101 80-81 0.14697316837854402 1101 82-83 -0.02059548255603616 1101 84-85 0.016828930552073018 1101 86-87 -0.037459164392299016 1101 88-89 0.038042667356414483 1101 90-91 0.10450737325702164 1101 92-93 0.022091057338816 1101 94-95 0.016171022087124953 1101 96-97 -0.17753302394957515 1101 98-99 -0.3051337866859285 1101 100-101 0.12130602693521908 1102 1 0.33680223709842494 1102 2 0.19622034433414015 1102 3 0.17936718572688903 1102 4 0.2152105976892713 1102 5 0.1719831579045561 1102 6 0.23090115300585978 1102 7 0.21824546240677734 1102 8 0.26068869553342466 1102 9 0.3480950468558319 1102 10-11 0.4568714641191818 1102 12-13 0.34279132986313954 1102 14-15 0.28975192877827993 1102 16-17 0.09984550730877118 1102 18-19 0.22061142816999535 1102 20-21 0.11018732356704675 1102 22-23 -0.02655752814458623 1102 24-25 0.15247698127549825 1102 26-27 -0.0941589990650229 1102 28-29 -0.20211710259030014 1102 30-31 0.19955338338218098 1102 32-33 0.27862551743363895 1102 34-35 0.28962232466188453 1102 36-37 -0.14476788821693987 1102 38-39 -0.1456206070506525 1102 40-41 -0.1292663615680496 1102 42-43 -0.058087898954106265 1102 44-45 0.07751670602536365 1102 46-47 0.1629074620917379 1102 48-49 0.17867769127656885 1102 50-51 0.101772453562603 1102 52-53 0.23458730027429198 1102 54-55 0.18979485592068812 1102 56-57 0.24033821813964096 1102 58-59 0.2365094901167808 1102 60-61 0.20319529477178122 1102 62-63 0.20384795086211227 1102 64-65 0.16257623911710084 1102 66-67 0.15969051522115763 1102 68-69 0.1477843088788262 1102 70-71 0.2901915567007727 1102 72-73 -0.006839767989141876 1102 74-75 0.12074109878741623 1102 76-77 0.1620117908488652 1102 78-79 0.21595120952811797 1102 80-81 0.3205183711803059 1102 82-83 0.24795574728595682 1102 84-85 -0.12048919943652336 1102 86-87 -0.2634376625190278 1102 88-89 -0.11869751834158393 1102 90-91 -0.35242698087231616 1102 92-93 -0.2706012378232643 1102 94-95 -0.4005154089276992 1102 96-97 -0.5016734376977432 1102 98-99 -0.5773031953844132 1102 100-101 -0.3035830429458706 1103 1 0.25686848968641485 1103 2 0.2581333878123999 1103 3 0.29203799318030477 1103 4 0.3011733306085276 1103 5 0.44677404817370814 1103 6 0.4192821053868059 1103 7 0.6027672015372119 1103 8 0.498063436734256 1103 9 0.16364784188689185 1103 10-11 0.4207389589431969 1103 12-13 0.22783480812400825 1103 14-15 0.5113171461695813 1103 16-17 0.5312119669360982 1103 18-19 0.6289509726834552 1103 20-21 0.5504274891985119 1103 22-23 0.3750201116069647 1103 24-25 0.5007005837599721 1103 26-27 0.3637830299204836 1103 28-29 0.6037255475132213 1103 30-31 0.5309198430095066 1103 32-33 0.48246402675041367 1103 34-35 0.5256927180366304 1103 36-37 0.5277704140604911 1103 38-39 0.5832489581667417 1103 40-41 0.31264254112346634 1103 42-43 0.34878580705003515 1103 44-45 0.3578521097520664 1103 46-47 0.6242946256528086 1103 48-49 0.5029592647755337 1103 50-51 0.5866213148462478 1103 52-53 0.6711463064854684 1103 54-55 0.56451121202835 1103 56-57 0.6215680318042445 1103 58-59 0.6351347489159522 1103 60-61 0.6361021270699112 1103 62-63 0.7132599591436843 1103 64-65 0.5159716842516744 1103 66-67 0.4753261259872019 1103 68-69 0.6280534600175471 1103 70-71 0.6598354490403189 1103 72-73 0.5040546419487448 1103 74-75 0.5262235004437308 1103 76-77 0.6178254554451392 1103 78-79 0.5102369238138316 1103 80-81 0.5389904208697445 1103 82-83 0.4014298673687726 1103 84-85 0.5309021049113021 1103 86-87 0.5719743457625448 1103 88-89 0.4569463739979618 1103 90-91 0.5731423773057394 1103 92-93 0.21098468350178834 1103 94-95 0.36597734469548726 1103 96-97 0.4910719039171667 1103 98-99 0.4215912145534766 1103 100-101 0.6316509115054778 1104 1 0.2222656517325703 1104 2 0.2840809715118411 1104 3 0.26693977573850347 1104 4 0.29346843741052453 1104 5 0.2947799058720335 1104 6 0.31772577553779513 1104 7 0.43168100247646635 1104 8 0.42139020540566463 1104 9 0.4380648494109991 1104 10-11 0.5025694896708686 1104 12-13 0.44797367597231386 1104 14-15 0.33090407744261796 1104 16-17 0.31524620417287963 1104 18-19 0.3045092214336478 1104 20-21 0.10022448965298736 1104 22-23 0.4891265601248733 1104 24-25 0.4399729162348436 1104 26-27 0.24427537956448475 1104 28-29 0.22628475571399775 1104 30-31 0.3276950790848545 1104 32-33 0.21754305518044958 1104 34-35 0.3043308031752403 1104 36-37 0.1544794985426421 1104 38-39 0.030532703053872012 1104 40-41 -0.2142442942045193 1104 42-43 -0.034452591172460245 1104 44-45 -0.01664821848102349 1104 46-47 -0.17033295602673348 1104 48-49 -0.10965448061657668 1104 50-51 -0.1280254558450622 1104 52-53 -0.11810027231572207 1104 54-55 -0.1626023566228625 1104 56-57 -0.11723669475234288 1104 58-59 -0.03408052149762142 1104 60-61 0.03517903460917893 1104 62-63 0.01689324702935835 1104 64-65 0.0758863436467152 1104 66-67 0.2118438253256869 1104 68-69 0.1307111381471202 1104 70-71 0.0965283743546621 1104 72-73 -0.1327700815779913 1104 74-75 -0.24750977229272308 1104 76-77 -0.34729599080037943 1104 78-79 -0.10950755934528544 1104 80-81 -0.029488597460812116 1104 82-83 -0.06257154655840935 1104 84-85 -0.27031033764790635 1104 86-87 -0.24953754637500936 1104 88-89 -0.3184396786203294 1104 90-91 -0.2744595011975193 1104 92-93 -0.4376604712727392 1104 94-95 -0.49376511856765504 1104 96-97 -0.2762355747476839 1104 98-99 -0.22869460072704229 1104 100-101 -0.489871080111957 1105 1 0.2980737646470857 1105 2 0.20371226119326025 1105 3 0.26115537444348647 1105 4 0.32886285766947765 1105 5 0.25780895797053915 1105 6 0.3656177481889671 1105 7 0.36975585501647856 1105 8 0.39668143720284377 1105 9 0.42349722435501036 1105 10-11 0.543547808559957 1105 12-13 0.33906384718415694 1105 14-15 0.4003085107644182 1105 16-17 0.4242549431385285 1105 18-19 0.4423306627460448 1105 20-21 0.3599438395683592 1105 22-23 0.1661211257650166 1105 24-25 0.29554494564369094 1105 26-27 0.17316432657008107 1105 28-29 0.0942319571259631 1105 30-31 0.0750251748701416 1105 32-33 0.17912634224393287 1105 34-35 0.23600767603107187 1105 36-37 0.4617867142245018 1105 38-39 0.2416288155821391 1105 40-41 0.31833805941512594 1105 42-43 0.28259174472784565 1105 44-45 0.4242930174737296 1105 46-47 0.5310384419663592 1105 48-49 0.580604778046613 1105 50-51 0.504510493813342 1105 52-53 0.6977202596936181 1105 54-55 0.6154401874944284 1105 56-57 0.4734833847513187 1105 58-59 0.5921633997175704 1105 60-61 0.6610303327130467 1105 62-63 0.711336238555937 1105 64-65 0.584156575639696 1105 66-67 0.748904965897502 1105 68-69 0.7414992544413437 1105 70-71 0.471633655018163 1105 72-73 0.4528200802457647 1105 74-75 0.6643260542476597 1105 76-77 0.6462269013734456 1105 78-79 0.8475925820124459 1105 80-81 0.8944457878744672 1105 82-83 0.616994348407701 1105 84-85 0.649226735898349 1105 86-87 0.5281915027729518 1105 88-89 0.413836958728691 1105 90-91 0.427259591216103 1105 92-93 0.29350367804608624 1105 94-95 0.46255849407461014 1105 96-97 0.2952721248228798 1105 98-99 0.5547792856449512 1105 100-101 0.5974565479482266 1106 1 0.3680694993510727 1106 2 0.3075283513475817 1106 3 0.37326382094439126 1106 4 0.3246002612110246 1106 5 0.2068997480356387 1106 6 0.3648655848335238 1106 7 0.5233699510099328 1106 8 0.5279800038744114 1106 9 0.5282777303324977 1106 10-11 0.5721651102743124 1106 12-13 0.5859310142582288 1106 14-15 0.5738734951137516 1106 16-17 0.513093399101372 1106 18-19 0.3571524651575544 1106 20-21 0.413276911944763 1106 22-23 0.5714701916516916 1106 24-25 0.2515241550251801 1106 26-27 0.2812600541202528 1106 28-29 0.37542969543925864 1106 30-31 0.46880361298892836 1106 32-33 0.25438789532459793 1106 34-35 0.23958458566213636 1106 36-37 0.28409276136141415 1106 38-39 0.3280602813187201 1106 40-41 0.31250854032725073 1106 42-43 0.38724064742645936 1106 44-45 0.44806175069431475 1106 46-47 0.40310200162583953 1106 48-49 0.4477494120747707 1106 50-51 0.1558392482524269 1106 52-53 0.18835719256784955 1106 54-55 0.4041266585423813 1106 56-57 0.3112028753993883 1106 58-59 0.4366267147974199 1106 60-61 0.5648857013844477 1106 62-63 0.5654889294043173 1106 64-65 0.5912537042586195 1106 66-67 0.6149070135095442 1106 68-69 0.6084881579228281 1106 70-71 0.4242603552312936 1106 72-73 0.13525073881556793 1106 74-75 0.45761610922408735 1106 76-77 0.4080752458041985 1106 78-79 0.2267185135274481 1106 80-81 0.4976126351863144 1106 82-83 0.5745944715073819 1106 84-85 0.47778900044658457 1106 86-87 0.4575171675719787 1106 88-89 0.41213691432102095 1106 90-91 0.09613359356201556 1106 92-93 0.12749795229112237 1106 94-95 0.22918184414067966 1106 96-97 0.3708208430069426 1106 98-99 0.6984097544360743 1106 100-101 0.7002945557850317 1107 1 0.25941848679282487 1107 2 0.30660482807629563 1107 3 0.3622292181610831 1107 4 0.30678259671135777 1107 5 0.27726455306387265 1107 6 0.1929348910875106 1107 7 0.2506265234449998 1107 8 0.42825482706304996 1107 9 0.4752488240541055 1107 10-11 0.6037507730589553 1107 12-13 0.34112333214494583 1107 14-15 0.238317815829042 1107 16-17 0.4818689772786158 1107 18-19 0.3480579280477514 1107 20-21 0.41998212431677473 1107 22-23 0.3782989627689517 1107 24-25 0.3188304976963252 1107 26-27 0.2984057470844377 1107 28-29 0.3631256649103136 1107 30-31 0.4103047483891089 1107 32-33 0.25398514582578713 1107 34-35 0.3052489239610523 1107 36-37 0.330032209574604 1107 38-39 0.0953742588934432 1107 40-41 0.28951564478839487 1107 42-43 0.3464512584087842 1107 44-45 0.22482450490075934 1107 46-47 0.291391693205739 1107 48-49 0.39367695784001455 1107 50-51 0.16342464164832649 1107 52-53 0.35688752030525706 1107 54-55 0.481131829272492 1107 56-57 0.4076887193212926 1107 58-59 0.38943850975658023 1107 60-61 0.4964572946087955 1107 62-63 0.5636150440085501 1107 64-65 0.3570355333881423 1107 66-67 0.4427272685419936 1107 68-69 0.3881518420872041 1107 70-71 0.2819905950839505 1107 72-73 0.29329991092863494 1107 74-75 0.31303153966428 1107 76-77 0.2294998521315641 1107 78-79 0.19574747715096663 1107 80-81 0.23613519132535998 1107 82-83 0.16351242563328228 1107 84-85 -0.05851429926538998 1107 86-87 -0.07652966806054806 1107 88-89 -0.07566174293615546 1107 90-91 0.04223767563489744 1107 92-93 0.19825883878010941 1107 94-95 0.2927395825155408 1107 96-97 0.23950266856906666 1107 98-99 0.39690607851339266 1107 100-101 0.18390501833683004 1108 1 0.2599231162193014 1108 2 0.23877655735189052 1108 3 0.2638980394885131 1108 4 0.21313113868673383 1108 5 0.25413362282424856 1108 6 0.14811839730002418 1108 7 0.29559457483281193 1108 8 0.1921274106644475 1108 9 0.4078515979970021 1108 10-11 0.3665823685993175 1108 12-13 0.3185191405140273 1108 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48-49 -2.1740842134853366 2316 50-51 -1.7127989750088233 2316 52-53 -1.5466507949638029 2316 54-55 -1.7949670488412153 2316 56-57 -1.852963369161941 2316 58-59 -1.6437444781371866 2316 60-61 -2.1473761337996535 2316 62-63 -1.6069139538997952 2316 64-65 -1.6270428085019475 2316 66-67 -1.5409444054137609 2316 68-69 -1.7170093419148245 2316 70-71 -1.577014792505576 2316 72-73 -1.3776651648145375 2316 74-75 -1.0162112821649636 2316 76-77 -1.1757977329606568 2316 78-79 -2.0275408539639486 2316 80-81 -2.0776086129466798 2316 82-83 -1.8857585384283304 2316 84-85 -1.6807114216587458 2316 86-87 -1.4713106783920473 2316 88-89 -2.35590386754793 2316 90-91 -2.3976015840469174 2316 92-93 -3.0382520314740553 2316 94-95 -2.8685154089277027 2316 96-97 -3.4228797869040903 2316 98-99 -4.476128592209811 2316 100-101 -5.230725900088725 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3538.0 3 1248.0 4 1326.0 5 1370.0 6 1536.0 7 1783.0 8 2280.0 9 2945.0 10 3701.0 11 4949.0 12 6742.0 13 8206.0 14 9011.0 15 10754.0 16 13238.0 17 17939.0 18 14049.0 19 9701.0 20 9525.0 21 9938.0 22 10703.0 23 12312.0 24 13691.0 25 16145.0 26 18317.0 27 21752.0 28 26180.0 29 32080.0 30 39283.0 31 49180.0 32 62460.0 33 82099.0 34 110581.0 35 154249.0 36 214820.0 37 277932.0 38 212114.0 39 30876.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.74199182553053 17.903747795356384 13.885401999228128 22.468858379884956 2 15.74071329923122 19.94367685054015 40.54851845485422 23.767091395374415 3 19.438094428956333 25.4760865112499 29.390220690754354 25.69559836903941 4 12.4934136343039 16.25982685761858 37.128067929141245 34.11869157893628 5 13.441726455685862 37.31961194006255 33.723378187174106 15.515283417077482 6 31.18182447055568 35.61410270346836 18.232253856568274 14.971818969407687 7 28.646607133449237 32.734049839312995 20.76115589273484 17.858187134502923 8 26.923249193445336 33.49905742774601 19.894400290873453 19.68329308793521 9 27.22368647280589 16.59689426632262 20.435880426684303 35.743538834187184 10-11 25.146396463164216 26.257191361867548 27.18082804171632 21.415584133251908 12-13 26.534927517997815 23.834184945533927 27.68330302134048 21.947584515127783 14-15 23.152069489175908 24.99300965079871 26.67915762751665 25.17576323250873 16-17 22.052097492020632 28.331317152729223 27.183395147136174 22.43319020811397 18-19 21.934105338454273 26.470892385840965 30.03021319171051 21.564789083994253 20-21 22.79718326191342 25.491044839054833 30.072823273930943 21.638948625100802 22-23 22.24526583318899 25.617221546427533 30.082153509964915 22.05535911041856 24-25 22.226334417394323 25.763762509314176 30.16028451414981 21.84961855914169 26-27 21.92113060597011 25.88537672652536 30.18033339073464 22.01315927676989 28-29 21.62420051858254 26.24682882660411 30.219664979215544 21.90930567559781 30-31 21.7858862096147 25.866776334202374 30.222142613015624 22.1251948431673 32-33 21.55796767412824 26.078769288314536 30.127899503205718 22.235363534351507 34-35 22.402791323112705 26.048639404489638 29.71457226338321 21.833997009014443 36-37 22.397680959383205 25.742035706218857 29.748893523399484 22.111389810998453 38-39 22.017269621764004 26.056681894884143 29.804963575750094 22.121084907601762 40-41 22.256952035760477 25.966416603228875 29.799058047555953 21.977573313454695 42-43 22.08351923346038 26.18055498390695 29.32267597402063 22.413249808612047 44-45 22.139957749001677 26.235558535017756 29.27890136867947 22.3455823473011 46-47 22.527611830217207 26.493593897344315 28.701420827209077 22.277373445229404 48-49 22.58476410924219 26.654560840010472 28.350452157564522 22.41022289318282 50-51 22.36588221658514 26.871070176409074 28.388242204889508 22.374805402116277 52-53 22.676674666788717 26.91755154777502 28.121751562460386 22.28402222297588 54-55 22.21624408872721 26.790833304247556 28.316025649046395 22.676896957978844 56-57 22.437965452547136 27.127540830095043 27.932750276744443 22.501743440613375 58-59 22.645115914265 26.711318392643236 28.06805826566207 22.575507427429695 60-61 22.33681060659442 26.812628290441985 28.572820898146688 22.277740204816904 62-63 22.414041751478543 26.86708500240689 28.459583670550398 22.25928957556417 64-65 22.043547305317453 27.065392078195366 28.30607771661951 22.58498289986767 66-67 22.125438351242796 27.16607012368511 27.991087585286877 22.717403939785218 68-69 22.219440693039953 26.941631806054218 28.255739648868538 22.583187852037288 70-71 22.33007214151596 26.909707810389694 28.02856013440063 22.73165991369371 72-73 22.279930267372073 26.949243630179975 28.078249650447752 22.692576452000203 74-75 22.061711163090024 27.46883186431791 27.81787809664082 22.651578875951248 76-77 22.33094793252639 27.05607025718395 27.832124165990702 22.780857644298965 78-79 22.361564124196725 27.180016464950707 27.775462611417613 22.68295679943496 80-81 22.358131438292723 27.4995395188392 27.579048716765044 22.563280326103037 82-83 22.332247501208098 27.457311447156517 27.650969260816126 22.559471790819256 84-85 22.2781501181566 27.374032156509415 27.55811671290186 22.78970101243213 86-87 22.364927235262716 27.32832833920674 27.551014023402086 22.755730402128457 88-89 22.27046437499609 27.35705490439261 27.377802250131978 22.994678470479325 90-91 22.312877444174905 27.32387036424032 27.50673985398767 22.856512337597103 92-93 22.488616581160663 27.234578456175896 27.60952485711858 22.667280105544858 94-95 22.191904692751816 27.073345260569948 27.708696180485237 23.026053866193003 96-97 22.158188849345535 26.983963372325263 27.795756519356612 23.062091258972586 98-99 22.1037222092654 27.168247179256085 27.36651948596117 23.36151112551734 100-101 22.19361657753852 27.03276254199555 27.380225577509858 23.393395302956073 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 239.0 1 239.0 2 693.5 3 941.0 4 563.5 5 444.5 6 732.5 7 1439.5 8 2312.5 9 2704.0 10 2368.0 11 1940.5 12 1905.5 13 2013.0 14 2120.5 15 2327.5 16 2546.5 17 2645.0 18 2758.0 19 2923.5 20 3200.0 21 3692.0 22 4362.0 23 5148.5 24 5977.0 25 7176.0 26 8871.0 27 10894.0 28 12724.0 29 14856.0 30 17944.5 31 21296.0 32 25324.0 33 28106.0 34 30136.5 35 32344.0 36 34950.0 37 37792.5 38 39227.0 39 40108.0 40 41166.0 41 43491.5 42 45612.5 43 47517.5 44 49634.0 45 51932.5 46 53047.5 47 54905.0 48 61487.0 49 61471.5 50 55296.0 51 52161.5 52 50064.0 53 49807.5 54 49408.0 55 46613.5 56 42758.0 57 38772.0 58 35945.5 59 32414.0 60 27464.5 61 23098.0 62 19464.0 63 15461.0 64 11694.5 65 9099.0 66 7024.0 67 5768.0 68 4355.5 69 2914.5 70 2228.0 71 1791.5 72 1519.0 73 1378.5 74 1118.0 75 908.0 76 647.5 77 413.5 78 279.5 79 178.0 80 125.5 81 82.5 82 60.0 83 32.5 84 14.5 85 16.0 86 13.0 87 7.0 88 6.5 89 4.5 90 3.5 91 5.0 92 4.5 93 2.5 94 2.0 95 2.0 96 1.5 97 2.5 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011655756132606944 2 0.011985014780420699 3 0.012775235535173712 4 0.018109225629756552 5 0.02943572311454974 6 0.04596450723480027 7 0.005465693553708342 8 0.024826102045157165 9 0.03503312012738359 10-11 0.03924763081939966 12-13 0.03042349905799101 14-15 0.026241914230756315 16-17 0.02821746611763885 18-19 0.023179808806088387 20-21 0.051298497329383116 22-23 0.012248421698671704 24-25 0.002930401965542424 26-27 9.54850078659891E-4 28-29 3.292586478137555E-5 30-31 0.002238958805133537 32-33 0.0017779966981942797 34-35 0.0 36-37 2.634069182510044E-4 38-39 3.292586478137555E-5 40-41 9.877759434412665E-4 42-43 0.0033255123429189308 44-45 0.001251182861692271 46-47 7.243690251902621E-4 48-49 6.914431604088866E-4 50-51 0.0030950312894493016 52-53 0.002930401965542424 54-55 0.0043462141511415725 56-57 9.219242138785153E-4 58-59 0.004609621069392577 60-61 0.0023706622642590395 62-63 3.9511037737650657E-4 64-65 4.938879717206332E-4 66-67 0.01524467539377688 68-69 0.04036711022196642 70-71 0.04695228317824154 72-73 0.01264353207604821 74-75 0.02805283679373197 76-77 0.024332214073436532 78-79 0.052253347408043 80-81 0.07431367681156462 82-83 0.044746250237889376 84-85 0.01573856336549751 86-87 0.018405558412788934 88-89 0.0195250378153557 90-91 0.03559285982866697 92-93 0.02278469842871188 94-95 0.012478902752141334 96-97 0.016956820362408406 98-99 0.046162062423488526 100-101 0.06529198986146773 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1518563.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.72264848408715 #Duplication Level Percentage of deduplicated Percentage of total 1 82.15453878009684 50.70795718496224 2 10.833695009316955 13.373686976877593 3 3.037176246038527 5.623876854553663 4 1.2626609928764596 3.1173912247152873 5 0.6542942338642409 2.0192386500983828 6 0.42796232632596615 1.5848980939349884 7 0.278162884372299 1.2018264953401836 8 0.19846104034712012 0.9799632824905227 9 0.14888923627372708 0.8270854194228711 >10 0.8923922312710106 10.278205051526973 >50 0.07096287247883529 3.0057185799399706 >100 0.03684166890602295 4.22541510300936 >500 0.002998631872874686 1.2838184845933596 >1k 7.496579682186715E-4 0.8806008573654998 >5k 2.1418799091962043E-4 0.8903177411691875 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7502 0.49401967517975875 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5967 0.39293727030093584 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3767 0.2480634652628834 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2511 0.16535369293206803 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1693 0.1114869781497376 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1617 0.10648224670296853 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1609 0.10595543286646653 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.292586478137555E-5 0.0 18-19 0.0 0.0 0.0 6.58517295627511E-5 6.58517295627511E-5 20-21 0.0 0.0 0.0 6.58517295627511E-5 1.317034591255022E-4 22-23 0.0 0.0 0.0 6.58517295627511E-5 1.6462932390687776E-4 24-25 0.0 0.0 0.0 1.317034591255022E-4 1.975551886882533E-4 26-27 0.0 0.0 0.0 3.6218451259513104E-4 1.975551886882533E-4 28-29 0.0 0.0 0.0 7.243690251902621E-4 1.975551886882533E-4 30-31 0.0 0.0 0.0 0.0017121449686315286 2.634069182510044E-4 32-33 0.0 0.0 0.0 0.0032596606133561794 3.292586478137555E-4 34-35 0.0 0.0 0.0 0.005004731446769084 3.292586478137555E-4 36-37 0.0 0.0 0.0 0.007869281682748757 3.292586478137555E-4 38-39 0.0 0.0 0.0 0.01073383191872843 3.292586478137555E-4 40-41 0.0 0.0 0.0 0.0149483426107445 3.292586478137555E-4 42-43 0.0 0.0 0.0 0.02202740353874024 3.292586478137555E-4 44-45 0.0 0.0 0.0 0.031510052595776404 3.292586478137555E-4 46-47 0.0 0.0 0.0 0.052055792219354746 3.6218451259513104E-4 48-49 0.0 0.0 0.0 0.08116225668609073 3.951103773765066E-4 50-51 0.0 0.0 0.0 0.10918216761504132 3.951103773765066E-4 52-53 0.0 0.0 0.0 0.1382886320817773 3.951103773765066E-4 54-55 0.0 0.0 0.0 0.172630309048752 3.951103773765066E-4 56-57 0.0 0.0 0.0 0.207959761959168 3.951103773765066E-4 58-59 0.0 0.0 0.0 0.25056583098626795 3.951103773765066E-4 60-61 0.0 0.0 0.0 0.3059471355485416 3.951103773765066E-4 62-63 0.0 0.0 0.0 0.36287595575553994 3.951103773765066E-4 64-65 0.0 0.0 0.0 0.42296565898155036 3.951103773765066E-4 66-67 0.0 0.0 0.0 0.5096265350861308 3.951103773765066E-4 68-69 0.0 0.0 0.0 0.6088321656724153 3.951103773765066E-4 70-71 0.0 0.0 0.0 0.7094865343090804 3.951103773765066E-4 72-73 0.0 0.0 0.0 0.8169565569554902 3.951103773765066E-4 74-75 0.0 0.0 0.0 0.9223193242558919 3.951103773765066E-4 76-77 0.0 0.0 0.0 1.0281759795280143 3.951103773765066E-4 78-79 0.0 0.0 0.0 1.1603404007604556 3.951103773765066E-4 80-81 0.0 0.0 0.0 1.3195369569784066 3.951103773765066E-4 82-83 0.0 0.0 0.0 1.4921014142975957 3.951103773765066E-4 84-85 0.0 0.0 0.0 1.6736217068373191 3.951103773765066E-4 86-87 0.0 0.0 0.0 1.9105562298040977 3.951103773765066E-4 88-89 0.0 0.0 0.0 2.186409124942462 3.951103773765066E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1695 0.0 38.952896 2 GTACCTG 1910 0.0 35.066685 1 GTATCAA 11660 0.0 28.802475 1 GTACATG 7880 0.0 28.332222 1 GTATAGG 450 0.0 27.445414 1 TACATGG 8090 0.0 27.361006 2 ACATGGG 8050 0.0 26.434847 3 CATGGGG 3170 0.0 26.072618 4 GTATACG 95 0.0012264834 25.000885 1 GTCCGTA 95 0.0012267189 25.00006 2 ATCAACG 13485 0.0 24.69231 3 TCAACGC 13540 0.0 24.59201 4 TATCAAC 13545 0.0 24.582932 2 CAACGCA 13670 0.0 24.21995 5 GAGTACT 7005 0.0 24.077305 12-13 AACGCAG 13915 0.0 23.864143 6 GGTATCA 8190 0.0 23.141844 1 GTACACG 295 5.456968E-12 22.54317 1 GTACTTT 7590 0.0 22.315807 14-15 AGTACTT 7170 0.0 21.966055 12-13 >>END_MODULE