FastQCFastQC Report
Thu 26 May 2016
SRR936395_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936395_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1518563
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72100.47479097014743543No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65090.42862890772394696No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42950.282833178472016No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30940.2037452512671519No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18910.12452562060316233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG17200.036.4563182
GTACCTG19000.033.256961
GTACACG2950.027.3786131
GGTATCA88400.026.3884431
GTATCAA121000.025.8359871
GTACATG74350.025.560161
TACATGG76200.023.5648232
ACATGGG74500.022.953293
CATGGGG31650.022.210494
GAGTACT81650.022.13553612-13
ATCAACG144100.021.5911663
TATCAAC144000.021.574592
TCAACGC144950.021.5286754
CAACGCA146900.021.2745275
AACGCAG149250.020.9706846
ACCTGGG29000.020.801973
GTACTTT88300.020.52328514-15
AGTACTT83450.020.12123712-13
TGCACCG4500.020.053815
TACACGG3353.45608E-1119.852292
Produced by FastQC (version 0.11.5)