##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936395_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1518563 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.734596457308655 31.0 31.0 34.0 30.0 34.0 2 31.85601453479375 31.0 31.0 34.0 30.0 34.0 3 31.9626502160266 31.0 31.0 34.0 30.0 34.0 4 35.74589858965351 37.0 35.0 37.0 35.0 37.0 5 35.60281661017686 37.0 35.0 37.0 33.0 37.0 6 35.66057252810717 37.0 35.0 37.0 33.0 37.0 7 35.57980143069468 37.0 35.0 37.0 33.0 37.0 8 35.543746950241776 37.0 35.0 37.0 33.0 37.0 9 37.117588799411024 39.0 37.0 39.0 33.0 39.0 10-11 36.99722533737487 39.0 37.0 39.0 33.0 39.0 12-13 36.856632553275695 39.0 37.0 39.0 33.0 39.0 14-15 37.87254233113805 40.0 37.0 41.0 33.0 41.0 16-17 37.846820645570844 39.5 37.0 41.0 32.5 41.0 18-19 38.007456391338394 40.0 37.0 41.0 33.0 41.0 20-21 38.01361747915628 40.0 37.0 41.0 33.0 41.0 22-23 37.89627200188599 40.0 37.0 41.0 33.0 41.0 24-25 37.76384153966612 39.5 37.0 41.0 32.0 41.0 26-27 37.525731234068 39.0 36.5 41.0 32.0 41.0 28-29 37.518365059599105 39.0 36.0 41.0 32.0 41.0 30-31 37.350819821107194 39.0 36.0 41.0 32.0 41.0 32-33 37.10552575033107 39.0 36.0 41.0 31.0 41.0 34-35 36.88343289017314 39.0 35.0 40.5 31.0 41.0 36-37 36.66018960029976 39.0 35.0 40.0 30.0 41.0 38-39 36.431787485932425 39.0 35.0 40.0 30.0 41.0 40-41 36.166893306369246 38.0 35.0 40.0 30.0 41.0 42-43 35.855181839673435 38.0 35.0 40.0 29.5 41.0 44-45 35.511535247467506 38.0 35.0 40.0 27.5 41.0 46-47 35.06550633724119 38.0 34.0 40.0 26.5 41.0 48-49 34.61371605919544 38.0 34.0 40.0 24.5 41.0 50-51 34.25915651836638 38.0 33.0 40.0 24.0 41.0 52-53 33.9566639645507 38.0 33.0 40.0 23.0 41.0 54-55 33.588683511978104 37.0 33.0 40.0 22.5 41.0 56-57 33.2135640734036 37.0 32.0 39.5 21.5 41.0 58-59 32.80807974381043 36.0 31.5 39.0 19.5 40.0 60-61 32.399180014263486 36.0 31.0 39.0 18.5 40.0 62-63 32.02641279946897 35.0 31.0 39.0 18.0 40.0 64-65 31.66374954479992 35.0 30.5 38.0 14.0 40.0 66-67 31.17146209936631 35.0 30.0 38.0 12.0 40.0 68-69 30.693842468175504 34.0 29.0 37.5 8.0 39.5 70-71 30.1584965523327 34.0 29.0 37.0 7.0 39.0 72-73 29.551726204312892 34.0 28.0 36.0 6.0 39.0 74-75 28.983794547871902 33.5 27.5 36.0 2.0 38.5 76-77 27.71042689700724 31.5 26.0 34.5 2.0 36.0 78-79 28.47137128983124 33.0 27.0 35.0 2.0 37.0 80-81 28.301625615795984 33.0 27.0 35.0 2.0 36.5 82-83 27.844988979713058 33.0 26.0 35.0 2.0 36.0 84-85 27.348640787375963 32.5 26.0 35.0 2.0 36.0 86-87 26.85513804827327 32.0 25.0 34.5 2.0 35.0 88-89 26.271911339865383 32.0 24.5 34.0 2.0 35.0 90-91 25.598475005646783 31.0 22.5 34.0 2.0 35.0 92-93 24.900954389116553 31.0 19.5 34.0 2.0 35.0 94-95 24.02948280710119 31.0 14.0 34.0 2.0 35.0 96-97 23.052261578874237 30.0 2.0 34.0 2.0 35.0 98-99 21.76112416804571 30.0 2.0 34.0 2.0 35.0 100-101 17.996243488087092 23.5 2.0 31.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2183017192014205 1101 2 0.3408571837203489 1101 3 0.3178113822179114 1101 4 0.24749760740670013 1101 5 0.23156092795098715 1101 6 0.24823058479518778 1101 7 0.25574238530276716 1101 8 0.23569308688446 1101 9 0.3746779208658282 1101 10-11 0.29017625420673454 1101 12-13 0.3701038246333823 1101 14-15 0.38600414746886 1101 16-17 0.3267271104139198 1101 18-19 0.31265042833931034 1101 20-21 0.22584660075673924 1101 22-23 0.16742943718735148 1101 24-25 0.2636071326665288 1101 26-27 0.22645426981148375 1101 28-29 0.23785760398313727 1101 30-31 0.3826943524805202 1101 32-33 0.2985998834224617 1101 34-35 0.2703666838967962 1101 36-37 0.4459963739280184 1101 38-39 0.43599051579920456 1101 40-41 0.22646621196877703 1101 42-43 0.18588233462816817 1101 44-45 0.4148840602582169 1101 46-47 0.3776801821736555 1101 48-49 0.41904897645773076 1101 50-51 0.4805812981726518 1101 52-53 0.45269019035858094 1101 54-55 0.4609531422583757 1101 56-57 0.5656642345296135 1101 58-59 0.33166169378408483 1101 60-61 0.4780685509988132 1101 62-63 0.43092013139211716 1101 64-65 0.5366373780561808 1101 66-67 0.363773883089074 1101 68-69 0.11173026378313367 1101 70-71 0.03114090967508787 1101 72-73 0.19284200001964535 1101 74-75 0.2744949705287745 1101 76-77 0.3474536242778754 1101 78-79 0.41712840570538745 1101 80-81 0.5291364772035188 1101 82-83 0.47156594559465503 1101 84-85 0.4780950735367888 1101 86-87 0.4139476505595354 1101 88-89 0.2991900397899272 1101 90-91 0.2812409400896989 1101 92-93 0.45227678168223306 1101 94-95 0.318911551276873 1101 96-97 0.434420292164301 1101 98-99 0.17333683466950944 1101 100-101 0.530430527884274 1102 1 0.20670895164970204 1102 2 0.30804923454295974 1102 3 0.2532340894992302 1102 4 0.1920077882179072 1102 5 0.06254512733199391 1102 6 0.1319595308776087 1102 7 0.2244591144117436 1102 8 0.12997945274209144 1102 9 0.27456454735222025 1102 10-11 0.24447109049928883 1102 12-13 0.26681371223723005 1102 14-15 0.5201940806827423 1102 16-17 0.5392962584836383 1102 18-19 0.33526746694494847 1102 20-21 0.32373518195888806 1102 22-23 0.24296551798227028 1102 24-25 0.31700442864306666 1102 26-27 0.18178575702437882 1102 28-29 0.3777852795003227 1102 30-31 0.44972383610977573 1102 32-33 0.4488572543297735 1102 34-35 0.4697493195051976 1102 36-37 0.5230196676240624 1102 38-39 0.5441755622236997 1102 40-41 0.7061143631334588 1102 42-43 0.7166951705949387 1102 44-45 0.7705504554365774 1102 46-47 0.6074117345437529 1102 48-49 0.8509310419407115 1102 50-51 0.693543018322508 1102 52-53 0.8531752856509698 1102 54-55 0.8917320964854483 1102 56-57 0.9432475542885399 1102 58-59 0.7329433357453183 1102 60-61 0.5927709455256007 1102 62-63 0.6283803040586697 1102 64-65 0.5289612092990517 1102 66-67 0.7993995550226138 1102 68-69 0.5699729743223081 1102 70-71 0.38484705074040804 1102 72-73 0.30099674834999135 1102 74-75 0.4294983912813386 1102 76-77 0.5897158819745698 1102 78-79 0.7007589644283065 1102 80-81 0.8666197806731404 1102 82-83 0.587597221046682 1102 84-85 0.5324127148464477 1102 86-87 0.5469641027504437 1102 88-89 0.5366459772390222 1102 90-91 0.44593551575348656 1102 92-93 0.4997841933127951 1102 94-95 0.6327346495013373 1102 96-97 0.7634373959271308 1102 98-99 0.6770801153341104 1102 100-101 0.5212010116764212 1103 1 0.3776737549623306 1103 2 0.4221610357851908 1103 3 0.37994216403338754 1103 4 0.24125002424275266 1103 5 0.14539398861563768 1103 6 0.20681667373475676 1103 7 0.1737676030245794 1103 8 0.1955529517068939 1103 9 0.18978607944332282 1103 10-11 0.33502595592371875 1103 12-13 0.32249901244426127 1103 14-15 0.41240112002021334 1103 16-17 0.48910164150640867 1103 18-19 0.4293627050401909 1103 20-21 0.37038942626531224 1103 22-23 0.42619119086010926 1103 24-25 0.46888434582734106 1103 26-27 0.449653251848396 1103 28-29 0.49515173912764965 1103 30-31 0.5889619313478747 1103 32-33 0.5600580824871244 1103 34-35 0.6466230255093421 1103 36-37 0.6985227732141297 1103 38-39 0.6169167630518615 1103 40-41 0.7380812368394629 1103 42-43 1.0009187730794125 1103 44-45 0.7753289233454836 1103 46-47 0.9165752956203548 1103 48-49 0.9010801102636918 1103 50-51 0.8309881528980796 1103 52-53 0.8305334637048034 1103 54-55 0.9986389287835848 1103 56-57 0.9016574921767315 1103 58-59 0.8176100024119819 1103 60-61 0.6461332643661848 1103 62-63 0.965572850642527 1103 64-65 1.046555412197602 1103 66-67 0.7321076295567757 1103 68-69 0.8101096202850435 1103 70-71 0.614631729829437 1103 72-73 1.1149222141884998 1103 74-75 1.0162230289624965 1103 76-77 0.7756868964673238 1103 78-79 0.8091937470370034 1103 80-81 0.893162223737324 1103 82-83 0.9209264135932678 1103 84-85 1.1445617831694257 1103 86-87 1.1592498170361552 1103 88-89 0.9454782753756703 1103 90-91 0.9898278552979995 1103 92-93 0.9240160773707622 1103 94-95 1.1175379621307364 1103 96-97 1.3543649321590117 1103 98-99 1.4253616888330747 1103 100-101 1.184575752877251 1104 1 0.21870895164969895 1104 2 0.2558912786776837 1104 3 0.25678809646787215 1104 4 0.15350372317725913 1104 5 0.14258229341793793 1104 6 0.1391743973119901 1104 7 0.08619198316900167 1104 8 0.23802126458878092 1104 9 0.25238568556360264 1104 10-11 0.24814821012763133 1104 12-13 0.18568943813734506 1104 14-15 0.23423821540980327 1104 16-17 0.23059939437213473 1104 18-19 0.2517552718230007 1104 20-21 0.2359744386371716 1104 22-23 0.21766470497414048 1104 24-25 -3.7014382314737304E-6 1104 26-27 0.083388544480826 1104 28-29 0.25062616219486245 1104 30-31 0.3887412577125673 1104 32-33 0.45748675491049084 1104 34-35 0.25024873878510334 1104 36-37 0.23799760026053463 1104 38-39 0.32948102099257426 1104 40-41 0.22679961284310224 1104 42-43 0.32082989765650183 1104 44-45 0.4821485971322801 1104 46-47 0.4951260202580343 1104 48-49 0.4802272091880866 1104 50-51 0.4239402308660658 1104 52-53 0.4479232531306465 1104 54-55 0.41560201518463913 1104 56-57 0.45077833245346 1104 58-59 0.45038584445612173 1104 60-61 0.17222274575789243 1104 62-63 0.16230364900641447 1104 64-65 0.1989681779401664 1104 66-67 0.3697317269157594 1104 68-69 0.24901130184844078 1104 70-71 0.08130698105399503 1104 72-73 0.23910592372745754 1104 74-75 0.38573067931850247 1104 76-77 0.24389474376318887 1104 78-79 0.4893280701193632 1104 80-81 0.6480483521017106 1104 82-83 0.35799907935097863 1104 84-85 0.49432444539232634 1104 86-87 0.5133845440977147 1104 88-89 0.615033898261089 1104 90-91 0.5673757015839165 1104 92-93 0.42736607484589584 1104 94-95 0.6069622917777586 1104 96-97 1.004536118342923 1104 98-99 0.9433170491320233 1104 100-101 0.865091836298955 1105 1 0.0851721651106061 1105 2 0.2312864499834042 1105 3 0.13504207366287346 1105 4 0.131106435529027 1105 5 0.11535014217857764 1105 6 0.07351413331905121 1105 7 0.06457524770108591 1105 8 0.13148192717297036 1105 9 0.19333195084941934 1105 10-11 0.21892209676784802 1105 12-13 0.13602915268592852 1105 14-15 0.21745801997670355 1105 16-17 0.2864430747158977 1105 18-19 0.1956983478423453 1105 20-21 0.14179786754120727 1105 22-23 0.07651418178959091 1105 24-25 0.052982983575439846 1105 26-27 0.2527623324120398 1105 28-29 0.059337902397054165 1105 30-31 0.15631374043178425 1105 32-33 0.22757055027170736 1105 34-35 0.12937815487306636 1105 36-37 0.31094972371116825 1105 38-39 0.01669552263280849 1105 40-41 0.06313679797344918 1105 42-43 0.08257606798854766 1105 44-45 -0.005561058915120043 1105 46-47 -0.015555273044505213 1105 48-49 0.19957142465268873 1105 50-51 0.1311335824927511 1105 52-53 0.20293213157641077 1105 54-55 0.08984888962302762 1105 56-57 0.26759232499127705 1105 58-59 0.27007728493700256 1105 60-61 0.24351921342398697 1105 62-63 0.24215232649351393 1105 64-65 0.14091963886025027 1105 66-67 0.07257705419780436 1105 68-69 0.12361599642722254 1105 70-71 0.1752931081472049 1105 72-73 0.3416523075713691 1105 74-75 0.38977090860235464 1105 76-77 0.01937869952653415 1105 78-79 0.09377051177241569 1105 80-81 0.10709652102285716 1105 82-83 0.19893374364648153 1105 84-85 0.4918406264267823 1105 86-87 0.4019865375904317 1105 88-89 0.6125638261337798 1105 90-91 0.5105627015007634 1105 92-93 0.6833731078624474 1105 94-95 0.7404169325762275 1105 96-97 1.2137445552804778 1105 98-99 1.1127300658454935 1105 100-101 0.790630242953231 1106 1 0.08966996024951612 1106 2 0.2890164216515849 1106 3 0.1609672447133903 1106 4 0.15448052763512976 1106 5 0.16238515404987197 1106 6 0.07634326637063538 1106 7 0.25807053630370547 1106 8 0.18382999962361168 1106 9 0.25507953441075415 1106 10-11 0.2801947274701533 1106 12-13 0.17722232875221522 1106 14-15 0.3330807801901301 1106 16-17 0.2442210309642192 1106 18-19 0.15929451879432577 1106 20-21 0.13797631078914918 1106 22-23 0.014607999404987027 1106 24-25 0.10779861750505404 1106 26-27 -0.140334974377474 1106 28-29 0.1574305996973706 1106 30-31 0.05313103323761226 1106 32-33 0.11513512023943662 1106 34-35 0.09240691322649752 1106 36-37 0.25378463380925353 1106 38-39 0.24237928602765635 1106 40-41 0.2681849985179454 1106 42-43 0.34758103516870875 1106 44-45 0.5076508979514003 1106 46-47 0.5763650617542382 1106 48-49 0.4881789337333089 1106 50-51 0.3294079260623448 1106 52-53 0.2691722798899292 1106 54-55 0.2643015212161828 1106 56-57 0.30808749751304987 1106 58-59 0.2274015473174984 1106 60-61 0.4621210331936325 1106 62-63 0.3892599901236373 1106 64-65 0.4238916593282731 1106 66-67 0.24152546301293043 1106 68-69 0.2157285057575571 1106 70-71 0.14933014333454864 1106 72-73 0.22778425774299293 1106 74-75 0.3359367314332964 1106 76-77 0.21978484749180893 1106 78-79 0.23685364590113167 1106 80-81 0.23268974811072596 1106 82-83 0.36363980266144225 1106 84-85 0.3316021612854563 1106 86-87 0.26249937869600615 1106 88-89 0.27172938710793915 1106 90-91 0.5559730877665103 1106 92-93 0.3624072207821101 1106 94-95 0.5923171826899498 1106 96-97 0.5294724631392889 1106 98-99 0.979105664302967 1106 100-101 0.7243929629162977 1107 1 0.0767889777274462 1107 2 0.23084379081383943 1107 3 0.12970381486798033 1107 4 0.060374506496778224 1107 5 0.04148995660425925 1107 6 0.10041621737423156 1107 7 -0.014187124688575636 1107 8 0.030758851324108605 1107 9 0.039509865582196824 1107 10-11 0.13972217028088352 1107 12-13 0.1708371822070731 1107 14-15 0.18275719697317783 1107 16-17 0.28059134445869915 1107 18-19 0.2514809784761951 1107 20-21 0.18004012858100538 1107 22-23 0.12153499072287843 1107 24-25 0.22908706249523902 1107 26-27 0.1655344327639341 1107 28-29 0.21801639350896096 1107 30-31 0.25645384996358445 1107 32-33 0.254026352202807 1107 34-35 0.03169292638320087 1107 36-37 0.3319477093484622 1107 38-39 0.33549477011221995 1107 40-41 0.22523749898221013 1107 42-43 0.0963105238668831 1107 44-45 -0.060099369353579846 1107 46-47 0.24812088620212336 1107 48-49 0.22905947483126 1107 50-51 0.1632229140115271 1107 52-53 0.424930480826589 1107 54-55 0.3411389907890907 1107 56-57 0.47222025414999536 1107 58-59 0.4857180077361889 1107 60-61 0.37099912578801053 1107 62-63 0.0583524334694836 1107 64-65 0.21783628060538263 1107 66-67 0.3917038497011234 1107 68-69 0.3765512483016096 1107 70-71 0.18437618447034154 1107 72-73 -0.0021421971312278743 1107 74-75 0.13341624638686866 1107 76-77 0.10358011471844009 1107 78-79 0.14835394446253147 1107 80-81 0.2295209297237477 1107 82-83 0.319406038583967 1107 84-85 0.12827417682998643 1107 86-87 0.42090412393861243 1107 88-89 0.6189344622850683 1107 90-91 0.35357629482609454 1107 92-93 0.24051665195512228 1107 94-95 0.666770995357421 1107 96-97 0.9015879949003214 1107 98-99 1.0688802946186051 1107 100-101 0.5948698243241282 1108 1 -0.01163762485072084 1108 2 0.16001542220145382 1108 3 -0.06275914803246962 1108 4 0.02340592853912682 1108 5 0.15555949757712995 1108 6 0.04137288675250517 1108 7 0.03329766048106109 1108 8 0.11972755079787589 1108 9 0.14498889223810352 1108 10-11 0.0018658495863590474 1108 12-13 0.034823855939684734 1108 14-15 0.08145866225501663 1108 16-17 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2316 58-59 -1.3121677753657934 2316 60-61 -1.0270068322521766 2316 62-63 -1.161905410227039 2316 64-65 -0.9988165684787269 2316 66-67 -0.8819020322789761 2316 68-69 -1.4310111526618208 2316 70-71 -1.1618513619580035 2316 72-73 -0.8429715056182658 2316 74-75 -1.0851682753853318 2316 76-77 -1.314728562469874 2316 78-79 -1.5750188133494696 2316 80-81 -1.7030310129776538 2316 82-83 -1.501894842445381 2316 84-85 -1.3312063327725987 2316 86-87 -1.350972405186063 2316 88-89 -1.487004816411769 2316 90-91 -1.683524801706831 2316 92-93 -1.5113269177983177 2316 94-95 -2.0435828108161225 2316 96-97 -1.6812292707395393 2316 98-99 -1.2752373449833492 2316 100-101 -0.8787672422918327 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 11.0 4 15.0 5 76.0 6 150.0 7 392.0 8 825.0 9 1754.0 10 2677.0 11 4274.0 12 5946.0 13 8231.0 14 10043.0 15 13010.0 16 16072.0 17 20192.0 18 17740.0 19 13309.0 20 12591.0 21 13119.0 22 14177.0 23 16104.0 24 18411.0 25 21631.0 26 26494.0 27 32076.0 28 39028.0 29 47256.0 30 56902.0 31 69149.0 32 84191.0 33 104599.0 34 133327.0 35 172264.0 36 220897.0 37 229952.0 38 89382.0 39 2292.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.840407904130416 19.29918150469311 14.666397962059962 22.194012629116518 2 16.24849020493698 21.11177554533732 39.014882594911285 23.624851654814417 3 19.797535309818258 25.628273406357778 29.835619271024385 24.738572012799583 4 13.296684554238592 17.31483597278736 36.28538634323724 33.10309312973681 5 13.366129287181918 37.721010484168374 33.67018334123597 15.242676887413731 6 31.293071146867135 36.2974733349884 17.461508017777334 14.947947500367123 7 28.38866107758879 33.121509432968274 20.474371393368838 18.015458096074102 8 27.00842261924361 34.07380822242563 19.399485028283934 19.518284130046823 9 26.991408313528396 16.533744775481523 20.19767398214494 36.27717292884513 10-11 25.33296280760166 26.623755484625928 26.753615095323667 21.289666612448745 12-13 26.622339672440326 24.033741109193365 27.54232126029674 21.801597958069568 14-15 22.99384316619105 25.378048166136725 26.56233073975186 25.06577792792036 16-17 21.92716266701438 28.633914759694886 27.159176743999204 22.279745829291535 18-19 21.80207126377514 26.628676266009194 30.15847772352616 21.410774746689505 20-21 22.644441100614632 25.589286896098024 30.346846978847935 21.41942502443941 22-23 22.2133358708275 25.65920246075472 30.345883941909385 21.7815777265084 24-25 22.258587190260744 25.89599128909019 30.420060379749042 21.42536114090002 26-27 21.9080977729223 26.03694488098754 30.449284338216582 21.60567300787358 28-29 21.623358685176726 26.432614023205513 30.397583502545007 21.54644378907275 30-31 21.800669321827638 25.932631875628886 30.433070451324685 21.833628351218792 32-33 21.645256269513514 26.180952374680523 30.26366826715065 21.91012308865531 34-35 22.36396173329536 26.12833603052907 29.93623862197718 21.57146361419839 36-37 22.456441771119927 25.79984616823242 29.90714119201181 21.836570868635835 38-39 22.032050450417227 26.133089580969404 30.058953651708514 21.775906316904855 40-41 22.311915936316108 26.009391773670238 29.949432456868763 21.729259833144887 42-43 22.127043362904413 26.282930793269475 29.455771628687867 22.134254215138245 44-45 22.283101173793014 26.311659694855962 29.316277421870062 22.088961709480962 46-47 22.61881792194331 26.723718410102183 28.66667369085115 21.990789977103354 48-49 22.74134429476812 26.831039896321197 28.293138918010506 22.13447689090018 50-51 22.56955008277655 27.02028782796175 28.261310490429658 22.14885159883204 52-53 22.856995160537274 27.133785560897618 27.998981928238454 22.01023735032666 54-55 22.382249505370382 27.00550949402411 28.191501095939213 22.420739904666295 56-57 22.650382934219877 27.328147196370935 27.815796423488564 22.205673445920628 58-59 22.79594385511567 26.97227032229408 27.85588483114158 22.37590099144867 60-61 22.474024083944784 27.092429349064417 28.327676517034327 22.105870049956472 62-63 22.51086883512165 27.056320387313786 28.34510543527363 22.08770534229093 64-65 22.229970984944572 27.24185095770756 28.17357930935475 22.354598747993116 66-67 22.278258699309113 27.328818293976276 27.879671095110513 22.513251911604097 68-69 22.41690862072031 27.130781626286844 28.06697753564446 22.385332217348385 70-71 22.459122516218297 27.16929392868362 27.871993498851825 22.49959005624626 72-73 22.534557371608184 27.057267065979463 27.923074060988768 22.485101501423586 74-75 22.409051485410632 27.640900143499803 27.437800837554143 22.512247533535422 76-77 22.463900536532442 27.384326140604752 27.528970940981427 22.62280238188137 78-79 22.536888270137613 27.404977574473786 27.556445778828103 22.501688376560494 80-81 22.545171375465294 27.65955064873915 27.367194098210597 22.428083877584957 82-83 22.55139032943152 27.57350060091207 27.42467196786355 22.450437101792858 84-85 22.423552367258146 27.60978558674305 27.254378302309558 22.712283743689248 86-87 22.502042236849427 27.481708768629137 27.260742198754013 22.755506795767424 88-89 22.41646696708859 27.517186017275908 27.152656086704845 22.91369092893066 90-91 22.393638809897936 27.51837719581312 27.237236723044035 22.85074727124491 92-93 22.554317852329724 27.45672895352999 27.294401821224817 22.69455137291547 94-95 22.27161123079714 27.30441705659289 27.31785086692527 23.106120845684703 96-97 22.27830971446634 27.203864187124644 27.384034875144874 23.133791223264144 98-99 22.14860696609147 27.396454263128923 26.968053861694923 23.486884909084683 100-101 22.055957954157073 27.493376831455095 26.976891513058987 23.47377370132885 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 520.0 1 401.5 2 616.0 3 844.0 4 608.0 5 493.0 6 702.0 7 1317.0 8 1961.5 9 2286.5 10 2150.5 11 1855.0 12 1847.0 13 1986.5 14 2214.0 15 2390.0 16 2562.5 17 2827.5 18 3061.5 19 3328.0 20 3644.5 21 4050.0 22 4686.0 23 5457.5 24 6399.0 25 7516.0 26 9129.5 27 11143.5 28 13019.0 29 15343.5 30 18533.5 31 21918.0 32 25713.0 33 28259.5 34 30279.5 35 32575.5 36 35082.5 37 37974.0 38 39475.0 39 39993.0 40 40896.5 41 43543.0 42 45777.5 43 47576.0 44 49549.5 45 51638.5 46 52745.5 47 54507.5 48 61257.5 49 61429.5 50 55190.0 51 51763.5 52 49282.5 53 49197.0 54 48991.5 55 46248.5 56 42602.5 57 38724.0 58 35781.0 59 32231.0 60 27144.0 61 22789.0 62 19503.0 63 15535.0 64 11661.0 65 8991.0 66 6854.0 67 5580.0 68 4200.0 69 2887.0 70 2181.5 71 1703.0 72 1449.5 73 1322.0 74 1101.0 75 899.0 76 659.5 77 417.5 78 272.0 79 188.5 80 141.0 81 91.0 82 59.5 83 38.5 84 24.0 85 13.0 86 9.5 87 8.0 88 3.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010997238836979435 2 0.009548500786598909 3 0.005004731446769084 4 2.634069182510044E-4 5 0.005465693553708342 6 0.0 7 3.9511037737650657E-4 8 0.0021731070755707863 9 1.317034591255022E-4 10-11 0.0 12-13 0.0 14-15 4.2803624215788215E-4 16-17 0.0036876968555140614 18-19 0.0014158121855991487 20-21 7.572948899716377E-4 22-23 2.9633278303237994E-4 24-25 4.938879717206332E-4 26-27 2.3048105346962883E-4 28-29 9.877759434412664E-5 30-31 7.243690251902621E-4 32-33 0.004214510692016071 34-35 6.914431604088866E-4 36-37 0.0013828863208177732 38-39 0.00539984182414559 40-41 0.0 42-43 0.0012182569969108952 44-45 0.0044120658807043234 46-47 0.0 48-49 0.0017779966981942797 50-51 0.0011194794025667686 52-53 3.9511037737650657E-4 54-55 1.6462932390687776E-4 56-57 0.010272869811789171 58-59 0.0018109225629756552 60-61 0.002699920912072795 62-63 6.58517295627511E-5 64-65 0.002699920912072795 66-67 5.597397012833843E-4 68-69 0.0015804415095060263 70-71 0.004082807232890569 72-73 0.0024694398586031664 74-75 0.006190062578898603 76-77 0.0010207018082226422 78-79 0.006091284984554477 80-81 0.0026669950472914196 82-83 0.002502365723384542 84-85 0.0015475156447246508 86-87 4.938879717206332E-4 88-89 0.0021072553460080353 90-91 0.006914431604088865 92-93 0.0016133673742874022 94-95 4.2803624215788215E-4 96-97 1.9755518868825328E-4 98-99 4.6096210693925767E-4 100-101 0.001975551886882533 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1518563.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.50383161043597 #Duplication Level Percentage of deduplicated Percentage of total 1 81.44938878905407 49.280001040658576 2 11.265289049579305 13.631863033972689 3 3.1336169839167103 5.687875029794965 4 1.3034647940282553 3.154584576320686 5 0.7052435141038879 2.1334967410846875 6 0.44192503301708885 1.604289466926138 7 0.27421850576195234 1.161388920796057 8 0.20117913015270608 0.9737686571434648 9 0.16479039226978975 0.8973405130418144 >10 0.941386160489087 10.615673644721095 >50 0.07586946680841197 3.135078688533368 >100 0.03882924249359222 4.295359131922262 >500 0.003817337304140726 1.5779428605301682 >1k 7.634674608281453E-4 0.946026633011061 >5k 2.1813356023661295E-4 0.9053110615428813 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7210 0.47479097014743543 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6509 0.42862890772394696 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4295 0.282833178472016 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3094 0.2037452512671519 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1891 0.12452562060316233 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.292586478137555E-5 0.0 18-19 0.0 0.0 0.0 6.58517295627511E-5 0.0 20-21 0.0 0.0 0.0 9.877759434412666E-5 0.0 22-23 0.0 0.0 0.0 1.317034591255022E-4 3.292586478137555E-5 24-25 0.0 0.0 0.0 1.975551886882533E-4 6.58517295627511E-5 26-27 0.0 0.0 0.0 3.6218451259513104E-4 6.58517295627511E-5 28-29 0.0 0.0 0.0 7.243690251902621E-4 6.58517295627511E-5 30-31 0.0 0.0 0.0 0.0017450708334129043 6.58517295627511E-5 32-33 0.0 0.0 0.0 0.003358438207700306 6.58517295627511E-5 34-35 0.0 0.0 0.0 0.0052022866354573365 6.58517295627511E-5 36-37 0.0 0.0 0.0 0.008033911006655634 6.58517295627511E-5 38-39 0.0 0.0 0.0 0.010898461242635308 6.58517295627511E-5 40-41 0.0 0.0 0.0 0.015047120205088627 6.58517295627511E-5 42-43 0.0 0.0 0.0 0.02219203286264712 6.58517295627511E-5 44-45 0.0 0.0 0.0 0.03183931124359016 6.58517295627511E-5 46-47 0.0 0.0 0.0 0.0523850508671685 6.58517295627511E-5 48-49 0.0 0.0 0.0 0.08129396014521623 6.58517295627511E-5 50-51 0.0 0.0 0.0 0.10928094520938544 6.58517295627511E-5 52-53 0.0 0.0 0.0 0.1381569286226518 6.58517295627511E-5 54-55 0.0 0.0 0.0 0.17269616077831476 6.58517295627511E-5 56-57 0.0 0.0 0.0 0.2079268360943866 6.58517295627511E-5 58-59 0.0 0.0 0.0 0.2499731654202032 9.877759434412666E-5 60-61 0.0 0.0 0.0 0.3049922854698818 1.317034591255022E-4 62-63 0.0 0.0 0.0 0.3613942918403781 1.317034591255022E-4 64-65 0.0 0.0 0.0 0.4205949967172913 1.317034591255022E-4 66-67 0.0 0.0 0.0 0.5063668744727745 1.317034591255022E-4 68-69 0.0 0.0 0.0 0.6046176549803992 1.317034591255022E-4 70-71 0.0 0.0 0.0 0.7045805804566554 1.317034591255022E-4 72-73 0.0 0.0 0.0 0.8111945306187494 2.634069182510044E-4 74-75 0.0 0.0 0.0 0.9157341512996168 2.634069182510044E-4 76-77 0.0 0.0 0.0 1.0210969186000185 2.634069182510044E-4 78-79 0.0 0.0 0.0 1.1519113793764237 2.634069182510044E-4 80-81 0.0 0.0 0.0 1.3091982354370546 2.634069182510044E-4 82-83 0.0 0.0 0.0 1.4793261787624221 2.634069182510044E-4 84-85 0.0 0.0 0.0 1.6582782538491982 2.634069182510044E-4 86-87 0.0 0.0 0.0 1.8901421936396448 2.634069182510044E-4 88-89 0.0 0.0 0.0 2.158850176120451 2.634069182510044E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1720 0.0 36.456318 2 GTACCTG 1900 0.0 33.25696 1 GTACACG 295 0.0 27.378613 1 GGTATCA 8840 0.0 26.388443 1 GTATCAA 12100 0.0 25.835987 1 GTACATG 7435 0.0 25.56016 1 TACATGG 7620 0.0 23.564823 2 ACATGGG 7450 0.0 22.95329 3 CATGGGG 3165 0.0 22.21049 4 GAGTACT 8165 0.0 22.135536 12-13 ATCAACG 14410 0.0 21.591166 3 TATCAAC 14400 0.0 21.57459 2 TCAACGC 14495 0.0 21.528675 4 CAACGCA 14690 0.0 21.274527 5 AACGCAG 14925 0.0 20.970684 6 ACCTGGG 2900 0.0 20.80197 3 GTACTTT 8830 0.0 20.523285 14-15 AGTACTT 8345 0.0 20.121237 12-13 TGCACCG 450 0.0 20.05381 5 TACACGG 335 3.45608E-11 19.85229 2 >>END_MODULE