FastQCFastQC Report
Thu 26 May 2016
SRR936394_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936394_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563926
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80110.51223651246926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62340.39861221055216167No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38670.24726233849939194No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25720.16445790913380812No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17290.11055510299080647No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17010.10876473695046952No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16320.1043527634939249No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG18850.036.0508162
GTACCTG20900.034.3348351
GTATCAA112950.029.2417161
GGTATCA77650.028.091541
GTACATG83150.026.4620231
TACATGG83250.025.630232
ACATGGG81350.024.8268623
ATCAACG133200.024.4743023
TCAACGC133400.024.4376094
TATCAAC135250.024.2438852
CAACGCA135600.024.147815
AACGCAG138350.023.7701156
GAGTACT72350.022.68761312-13
CATGGGG33850.022.1813954
GTACACG3450.022.0396751
TATCACG1100.002870267521.6006972
ACGCAGA151350.021.5672767
CGCAGAG153250.021.361898
ACCTGGG30700.021.2066763
AGTACTT74950.020.75959812-13
Produced by FastQC (version 0.11.5)