##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936394_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1563926 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.511411665257818 31.0 31.0 34.0 30.0 34.0 2 31.56796421314052 31.0 31.0 34.0 30.0 34.0 3 31.608386202416227 33.0 31.0 34.0 30.0 34.0 4 35.26633165507831 37.0 35.0 37.0 33.0 37.0 5 35.20463947782696 37.0 35.0 37.0 33.0 37.0 6 35.32443095133657 37.0 35.0 37.0 33.0 37.0 7 35.18279061797042 37.0 35.0 37.0 32.0 37.0 8 35.16600721517514 37.0 35.0 37.0 32.0 37.0 9 36.707392165613975 39.0 37.0 39.0 32.0 39.0 10-11 36.57992961303796 39.0 37.0 39.0 32.0 39.0 12-13 36.48857586612154 39.0 37.0 39.0 32.0 39.0 14-15 37.57682428708264 40.0 37.0 41.0 32.0 41.0 16-17 37.51872499082437 40.0 37.0 41.0 32.0 41.0 18-19 37.49284365117019 40.0 37.0 41.0 32.0 41.0 20-21 37.39943993513759 40.0 37.0 41.0 32.0 41.0 22-23 37.28577279231882 39.0 37.0 41.0 32.0 41.0 24-25 37.14324079272293 39.0 36.0 41.0 31.0 41.0 26-27 36.93905753852803 39.0 36.0 41.0 31.0 41.0 28-29 37.028713315080125 39.0 36.0 41.0 31.0 41.0 30-31 36.927877342022576 39.0 36.0 41.0 31.0 41.0 32-33 36.7372733748272 39.0 35.5 41.0 30.0 41.0 34-35 36.538841351828665 39.0 35.0 41.0 30.0 41.0 36-37 36.292632451919076 39.0 35.0 40.5 30.0 41.0 38-39 36.10417916192966 39.0 35.0 40.0 30.0 41.0 40-41 35.92878787103737 38.5 35.0 40.0 29.0 41.0 42-43 35.655012129729926 38.0 35.0 40.0 28.0 41.0 44-45 35.357771083798085 38.0 35.0 40.0 27.0 41.0 46-47 34.968651649758364 38.0 34.0 40.0 25.5 41.0 48-49 34.676181289907575 38.0 34.0 40.0 24.0 41.0 50-51 33.95446044122292 37.5 33.5 39.5 23.5 40.5 52-53 34.12506026499975 38.0 33.5 39.5 24.0 40.5 54-55 34.475091532463814 38.0 34.0 40.0 24.0 41.0 56-57 34.39563189051144 38.0 34.0 40.0 23.5 41.0 58-59 34.15117595078028 38.0 34.0 40.0 23.0 41.0 60-61 33.83528856224655 37.5 33.5 40.0 22.0 41.0 62-63 33.517548144861074 37.0 33.0 40.0 22.0 41.0 64-65 33.20755010147539 36.0 33.0 39.5 21.0 41.0 66-67 32.84818239481919 36.0 32.0 39.0 20.0 41.0 68-69 32.47501831928109 35.5 32.0 39.0 18.5 40.5 70-71 32.079411685719144 35.0 31.5 38.5 18.0 40.0 72-73 31.678165079421916 35.0 31.0 37.5 15.0 40.0 74-75 31.258474825535224 35.0 31.0 37.0 12.0 39.0 76-77 30.855189120201338 35.0 31.0 36.5 8.0 39.0 78-79 30.416561589231204 34.5 30.0 36.0 7.0 38.5 80-81 30.01859550899467 34.0 30.0 35.5 4.0 37.0 82-83 29.621541876022267 34.0 29.5 35.0 2.0 37.0 84-85 29.24713317637791 34.0 29.0 35.0 2.0 36.5 86-87 28.917338480209423 34.0 29.0 35.0 2.0 36.0 88-89 28.552709335352183 34.0 29.0 35.0 2.0 36.0 90-91 28.214293067574808 34.0 28.5 35.0 2.0 35.0 92-93 27.860200866281396 34.0 27.0 35.0 2.0 35.0 94-95 27.45833594428381 34.0 27.0 35.0 2.0 35.0 96-97 26.900438383913304 33.0 25.5 35.0 2.0 35.0 98-99 26.260822123297395 33.0 25.0 35.0 2.0 35.0 100-101 24.2405168147342 31.0 12.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23870849057196608 1101 2 0.36786519611551327 1101 3 0.23145075196926612 1101 4 0.21088954590754128 1101 5 0.2870890351463018 1101 6 0.24670281940283445 1101 7 0.30651766608524866 1101 8 0.31238142427805116 1101 9 0.5056487303314583 1101 10-11 0.35459706094459165 1101 12-13 0.5004376530481807 1101 14-15 0.5307461938421412 1101 16-17 0.7041664958268967 1101 18-19 0.7438522028131658 1101 20-21 0.7632949686219348 1101 22-23 0.5742387394011743 1101 24-25 0.49053330564053255 1101 26-27 0.5891668094213358 1101 28-29 0.6224244821830851 1101 30-31 0.37270816637508375 1101 32-33 0.414271082132565 1101 34-35 0.3132036858267 1101 36-37 0.29721010636792755 1101 38-39 0.2818897646280618 1101 40-41 0.2739482665485724 1101 42-43 0.09155126378593792 1101 44-45 0.05676294303340512 1101 46-47 0.17674950100269626 1101 48-49 0.01352518204607378 1101 50-51 0.17761662800013056 1101 52-53 0.20714232703985402 1101 54-55 0.19916751643884112 1101 56-57 0.24917684415787988 1101 58-59 0.18854862656847615 1101 60-61 0.19380103428736817 1101 62-63 0.36827479620577463 1101 64-65 0.3929686971369648 1101 66-67 0.3178210091377309 1101 68-69 0.16112796413067798 1101 70-71 0.1812773752080119 1101 72-73 0.2259264972665207 1101 74-75 0.189607612348059 1101 76-77 0.2141058440833099 1101 78-79 0.20803665323723308 1101 80-81 0.08143471085107734 1101 82-83 0.0957300261881393 1101 84-85 0.19224797120656234 1101 86-87 0.08225985296123284 1101 88-89 0.1623942184620617 1101 90-91 0.26874730914776634 1101 92-93 0.11837466072561753 1101 94-95 -0.0762695633142485 1101 96-97 -0.08803226469243697 1101 98-99 -0.08811851305326712 1101 100-101 0.20440111961386265 1102 1 0.2081064650620128 1102 2 0.23569262780526046 1102 3 0.19697625969798693 1102 4 0.32674497635861144 1102 5 0.3630978000201637 1102 6 0.2930074114871033 1102 7 0.12322631096987635 1102 8 0.25067217213467785 1102 9 0.3801569620257865 1102 10-11 0.42920324887941064 1102 12-13 0.492440436900317 1102 14-15 0.4540940844287391 1102 16-17 0.4559219822983849 1102 18-19 0.3653208024490695 1102 20-21 0.1573458041273028 1102 22-23 -0.08315954793840064 1102 24-25 0.04997280899826251 1102 26-27 0.23165037687252976 1102 28-29 0.29464115464678997 1102 30-31 0.020840034996076895 1102 32-33 0.11991226152442636 1102 34-35 9.590285908771534E-4 1102 36-37 -0.11572488642444512 1102 38-39 -0.019215032970372192 1102 40-41 -0.12169288740837203 1102 42-43 0.10343557177485252 1102 44-45 0.010954808818411266 1102 46-47 0.29462303348723395 1102 48-49 0.3210499127379478 1102 50-51 0.25937797722096434 1102 52-53 0.16526531887333817 1102 54-55 0.1270693707081918 1102 56-57 0.22650161185756446 1102 58-59 0.10662179139121974 1102 60-61 -0.198920190709309 1102 62-63 -0.053526969104936484 1102 64-65 0.25777954716567564 1102 66-67 0.08872549401381491 1102 68-69 0.21309770802009353 1102 70-71 0.22804090865270865 1102 72-73 -0.031170640605374444 1102 74-75 -0.004440806839788536 1102 76-77 0.19212530306981535 1102 78-79 0.03869603449462389 1102 80-81 0.06428426138422338 1102 82-83 -0.04492938050416839 1102 84-85 -0.09662994180727935 1102 86-87 0.03777846782628913 1102 88-89 -0.06402140977095527 1102 90-91 -0.25061725021447856 1102 92-93 -0.10120771694353792 1102 94-95 -0.19759572739111064 1102 96-97 -0.2236253530069341 1102 98-99 -0.35994644453921865 1102 100-101 -0.31144149799562015 1103 1 0.04850049669041212 1103 2 0.21791565026682136 1103 3 0.2556649077798703 1103 4 0.14439941548132396 1103 5 0.183824606793749 1103 6 -0.09406701246781779 1103 7 0.13934144872698084 1103 8 0.019628725076870523 1103 9 0.2873935998668742 1103 10-11 0.19417412214695418 1103 12-13 0.4282731891411231 1103 14-15 0.3981285122519651 1103 16-17 0.5620020127357606 1103 18-19 0.37432158628774204 1103 20-21 0.27607636721867834 1103 22-23 0.18527783913028628 1103 24-25 0.39499638778168134 1103 26-27 0.306623082490205 1103 28-29 0.28282535927958463 1103 30-31 0.19410846411212646 1103 32-33 0.09645245361579668 1103 34-35 0.28084892216187285 1103 36-37 0.41175877512011994 1103 38-39 0.28942271649646045 1103 40-41 0.22963893847764894 1103 42-43 0.3173474993563019 1103 44-45 0.3915383868892519 1103 46-47 0.7076331648565528 1103 48-49 0.8871878912485585 1103 50-51 0.30689819669579066 1103 52-53 0.4847288856446923 1103 54-55 0.8256563615311734 1103 56-57 0.8603706955298023 1103 58-59 0.751781778462977 1103 60-61 0.697179512042787 1103 62-63 0.6818094580158842 1103 64-65 0.7213288221148986 1103 66-67 0.7480203446009313 1103 68-69 0.555396302200343 1103 70-71 0.5993803438816769 1103 72-73 0.554742404200681 1103 74-75 0.3535730528530898 1103 76-77 0.356927701921542 1103 78-79 0.3828473382626463 1103 80-81 0.5758096165293125 1103 82-83 0.47365815747892626 1103 84-85 0.3683807596265325 1103 86-87 0.3944511490126352 1103 88-89 0.2863614750607475 1103 90-91 0.3617544852756005 1103 92-93 0.2407928022223338 1103 94-95 0.08795981826749255 1103 96-97 0.31116862212400065 1103 98-99 0.15715612634982534 1103 100-101 0.43540087917329373 1104 1 0.34488058718814685 1104 2 0.23827764121704575 1104 3 0.34729386705588894 1104 4 0.21396955122792605 1104 5 0.36764813620551706 1104 6 0.40197371151408134 1104 7 0.4651333039305996 1104 8 0.3985880010949714 1104 9 0.5984795727175509 1104 10-11 0.5991514977125618 1104 12-13 0.5648682117655639 1104 14-15 0.5205832633831804 1104 16-17 0.4016513340027217 1104 18-19 0.5977378758804974 1104 20-21 0.5294700323770485 1104 22-23 0.57187286175472 1104 24-25 0.4572135823518195 1104 26-27 0.3620529467435958 1104 28-29 0.44685250860084835 1104 30-31 0.42940258175918444 1104 32-33 0.3351402364212319 1104 34-35 0.33175389953743917 1104 36-37 0.46040130905677756 1104 38-39 0.4463797300711221 1104 40-41 0.3631231013735743 1104 42-43 0.4779809835191955 1104 44-45 0.25550897512455606 1104 46-47 0.5540698164402613 1104 48-49 0.4262150405698222 1104 50-51 0.18868552701253094 1104 52-53 0.32070173632342147 1104 54-55 0.4995817008976857 1104 56-57 0.5294883425886212 1104 58-59 0.40675915402858465 1104 60-61 0.43756412742740025 1104 62-63 0.19227325892085645 1104 64-65 0.2097337994904649 1104 66-67 0.17364251654662866 1104 68-69 0.3331112343270419 1104 70-71 0.30441428053325836 1104 72-73 -0.056117324306114824 1104 74-75 -0.1447301145677251 1104 76-77 0.00964263404824095 1104 78-79 0.0878812749142277 1104 80-81 0.24351323643881528 1104 82-83 0.1956604199223655 1104 84-85 0.2931545152826409 1104 86-87 0.09382897706693427 1104 88-89 0.07878228954038491 1104 90-91 0.0840395531489051 1104 92-93 0.14209370719970948 1104 94-95 0.11256389066568318 1104 96-97 0.2079446402235483 1104 98-99 -0.05211989174971876 1104 100-101 0.1520298384493124 1105 1 0.19759767765033587 1105 2 0.1773154552548597 1105 3 0.12564931697996684 1105 4 0.24748278817254032 1105 5 0.3047263085778056 1105 6 0.35402819298032284 1105 7 0.39763897908333234 1105 8 0.05066876346985083 1105 9 0.3088899865593362 1105 10-11 0.4326895555153243 1105 12-13 0.23872499223999455 1105 14-15 0.3144638109402038 1105 16-17 0.24401412734591332 1105 18-19 0.09278132190433297 1105 20-21 -0.11452078073141081 1105 22-23 -0.18621169167296614 1105 24-25 -0.13807470778941422 1105 26-27 -0.12099872380807142 1105 28-29 0.042652451214515 1105 30-31 -0.19382631352853963 1105 32-33 -0.1633140144840084 1105 34-35 -0.3915427293964484 1105 36-37 -0.6100775494122814 1105 38-39 -0.4774465797845977 1105 40-41 -0.7690340025972517 1105 42-43 -0.5289317206974218 1105 44-45 -0.4696000208177793 1105 46-47 -0.748800515841836 1105 48-49 -0.5250627575217024 1105 50-51 -0.5218264555582763 1105 52-53 -0.2965330169407423 1105 54-55 -0.2653216056722911 1105 56-57 -0.1703963769039447 1105 58-59 -0.37730477586868005 1105 60-61 -0.2911321490629959 1105 62-63 -0.5867087431003526 1105 64-65 -0.4799432627356168 1105 66-67 -0.7659059977567892 1105 68-69 -0.5907595118476294 1105 70-71 -0.38251534757284134 1105 72-73 -0.4890032473881121 1105 74-75 -0.4728961388121853 1105 76-77 -0.24583668148009608 1105 78-79 -0.09911754688851104 1105 80-81 -0.12800009953530633 1105 82-83 -0.3707683854770316 1105 84-85 -0.6375702156871874 1105 86-87 -0.3113455053134295 1105 88-89 -0.30520590817330273 1105 90-91 -0.21984805343478087 1105 92-93 -0.3502889555260893 1105 94-95 -0.2764087924246468 1105 96-97 -0.36257389353027847 1105 98-99 -0.36022418759911545 1105 100-101 0.09564776736135983 1106 1 0.09135800322447452 1106 2 0.1739882808370936 1106 3 0.07575691990541955 1106 4 0.23572288614527537 1106 5 0.3259959389068712 1106 6 0.28460342015105056 1106 7 0.41359678759739893 1106 8 0.4663780781982112 1106 9 0.5520985030896455 1106 10-11 0.4875461615837011 1106 12-13 0.5015202850410532 1106 14-15 0.48755508140596504 1106 16-17 0.4580701197246526 1106 18-19 0.40635382969689005 1106 20-21 0.3637049270095929 1106 22-23 0.4357024552459876 1106 24-25 0.2982460881678506 1106 26-27 0.2984321690792555 1106 28-29 0.16494902080864193 1106 30-31 0.25613929757383147 1106 32-33 0.20075690565538906 1106 34-35 0.038353138949119625 1106 36-37 0.11042053264571905 1106 38-39 0.09317500439728121 1106 40-41 0.04792396588023706 1106 42-43 0.1590520148435317 1106 44-45 0.17926002932136242 1106 46-47 0.5121121305295517 1106 48-49 0.28956910317671003 1106 50-51 0.3181320834770105 1106 52-53 0.39169471139084777 1106 54-55 0.44455817566354483 1106 56-57 0.3104960894665041 1106 58-59 0.29597139158905605 1106 60-61 0.34356061430836604 1106 62-63 0.4238744239552048 1106 64-65 0.32081938068908045 1106 66-67 0.30444957437797626 1106 68-69 0.29262893438944815 1106 70-71 0.3058692679612278 1106 72-73 0.46191890612107045 1106 74-75 0.2874566355817514 1106 76-77 0.11475535804058623 1106 78-79 0.39923716131424314 1106 80-81 0.23173707473350902 1106 82-83 0.27915166519316514 1106 84-85 0.29034359576191804 1106 86-87 0.4338764779350974 1106 88-89 0.41413567720021405 1106 90-91 0.4907442775172335 1106 92-93 0.35851884421265723 1106 94-95 0.41038460801560817 1106 96-97 0.2979100573460549 1106 98-99 0.408846431394565 1106 100-101 0.6744950009979753 1107 1 0.3328725413282001 1107 2 0.3166043787881563 1107 3 0.3153570408784745 1107 4 0.24068129701949204 1107 5 0.25731218881642093 1107 6 0.30011283359873175 1107 7 0.40525966612523945 1107 8 0.3917581135624175 1107 9 0.6019412566738396 1107 10-11 0.6202949773651198 1107 12-13 0.5640587153229077 1107 14-15 0.6068458304926381 1107 16-17 0.6429340157620445 1107 18-19 0.7546896152702871 1107 20-21 0.6989012206327132 1107 22-23 0.5789753700843008 1107 24-25 0.6769744980958095 1107 26-27 0.682103033312977 1107 28-29 0.6407477305530946 1107 30-31 0.4578820888229487 1107 32-33 0.6041014766185171 1107 34-35 0.5341107326986005 1107 36-37 0.6516910836853924 1107 38-39 0.6967498323539303 1107 40-41 0.6665380451398519 1107 42-43 0.6259289618695831 1107 44-45 0.7425884952828454 1107 46-47 0.745814444046438 1107 48-49 0.6386270162430918 1107 50-51 0.6225941708559262 1107 52-53 0.726389671898346 1107 54-55 0.7897189751989444 1107 56-57 0.9024990655520568 1107 58-59 0.9016608359479434 1107 60-61 1.0129353209208674 1107 62-63 1.0740409474456882 1107 64-65 0.7612709515761367 1107 66-67 0.7681611835236879 1107 68-69 0.6972786042183614 1107 70-71 0.7096339048658038 1107 72-73 0.8273130055304989 1107 74-75 0.5559435261475265 1107 76-77 0.621459899910807 1107 78-79 0.7613950459927814 1107 80-81 0.7725821791284275 1107 82-83 0.8374434217701499 1107 84-85 0.9361018389066231 1107 86-87 0.8212960225049457 1107 88-89 0.7464839088970052 1107 90-91 0.7208058958472527 1107 92-93 0.6023930990316337 1107 94-95 0.7185953757306471 1107 96-97 0.7518094901457104 1107 98-99 0.8840039097372383 1107 100-101 0.9074371510156958 1108 1 0.03984250699065939 1108 2 0.026607462081113198 1108 3 -0.18906355858262103 1108 4 -0.1339047086606584 1108 5 0.24335119151478324 1108 6 0.14289361912985044 1108 7 0.32969830572276493 1108 8 0.01946332011431906 1108 9 0.2729171284701408 1108 10-11 0.02523552343354396 1108 12-13 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-1.3490290544734407 2316 60-61 -1.630087768857429 2316 62-63 -1.677701363012936 2316 64-65 -1.509574369121104 2316 66-67 -2.151248826630315 2316 68-69 -1.7774154535748394 2316 70-71 -1.5490311082497819 2316 72-73 -1.4010245938941068 2316 74-75 -1.44306751633005 2316 76-77 -2.0120440770734582 2316 78-79 -2.072303813797369 2316 80-81 -2.110322870372329 2316 82-83 -2.019528464335796 2316 84-85 -2.118416692147701 2316 86-87 -2.186507486972122 2316 88-89 -2.392883211174073 2316 90-91 -2.804453242754885 2316 92-93 -2.859662663364709 2316 94-95 -2.847878699832055 2316 96-97 -2.8673876561565415 2316 98-99 -3.319618477722571 2316 100-101 -4.407121149881245 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3703.0 3 1347.0 4 1343.0 5 1408.0 6 1645.0 7 1866.0 8 2398.0 9 2902.0 10 3854.0 11 5034.0 12 6741.0 13 8429.0 14 8817.0 15 10846.0 16 13263.0 17 17252.0 18 14731.0 19 9598.0 20 9455.0 21 9895.0 22 10968.0 23 12151.0 24 13924.0 25 15991.0 26 18968.0 27 22356.0 28 27016.0 29 33145.0 30 40903.0 31 51219.0 32 65115.0 33 85173.0 34 114199.0 35 157965.0 36 218981.0 37 282919.0 38 222722.0 39 35666.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.81389533891016 17.872232663760375 13.822692237101858 22.49117976022761 2 15.720035349201252 19.922740765276956 40.50284366675065 23.854380218771144 3 19.467239977976455 25.430798813900413 29.424708447026788 25.67725276109634 4 12.538210455416818 16.242681726335423 37.06959467973578 34.149513138511985 5 13.451454357378683 37.30983707128779 33.75302824528419 15.485680326049339 6 31.167869855949963 35.58891871626827 18.207077363236916 15.036134064544857 7 28.666386959864205 32.75383712110068 20.79246991766131 17.787306001373803 8 26.923372103285914 33.4959727088186 19.8626615041616 19.71799368373389 9 27.19591688734925 16.621059289164418 20.44402123412988 35.73900258935646 10-11 25.19752937552795 26.26229930813943 27.08609667624821 21.454074640084414 12-13 26.578226634166658 23.833487442920546 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22.170849078592596 38-39 22.043594168942594 26.089582455415638 29.72420968223589 22.14261369340588 40-41 22.25969705175864 25.98818503777022 29.766741300157403 21.985376610313743 42-43 22.12306934839809 26.113759379505918 29.25667315678921 22.506498115306776 44-45 22.2012384979831 26.263619305580466 29.192047754650925 22.34309444178551 46-47 22.541492140863703 26.508547168405773 28.619002538876853 22.330958151853675 48-49 22.607682310607817 26.652940452421564 28.292541006798633 22.44683623017199 50-51 22.37088494157735 26.896094425142696 28.325688476701437 22.407332156578512 52-53 22.740324193121932 26.95893986464085 28.005992658801144 22.294743283436073 54-55 22.211067679442312 26.842220697531218 28.24257762869056 22.704133994335905 56-57 22.47016425644672 27.12597257106611 27.90700276744745 22.49686040503972 58-59 22.72546052528513 26.72861035613998 27.936429175004562 22.609499943570327 60-61 22.434694424320472 26.80417183264841 28.46938168713557 22.29175205589555 62-63 22.435864849803806 26.81336999797622 28.44368054443222 22.307084607787754 64-65 22.127372508396597 27.056179272353493 28.26855044592687 22.54789777332304 66-67 22.139035620860074 27.18255858639403 27.95285081338997 22.725554979355923 68-69 22.233878063628318 27.006801242077156 28.220582517815213 22.538738176479313 70-71 22.294487248887382 26.922546890822186 28.000649713783808 22.78231614650662 72-73 22.37516209807989 26.96093915854791 28.004953103458856 22.658945639913345 74-75 22.185905701452196 27.517475038741946 27.695974130899977 22.600645128905885 76-77 22.34374040842217 27.1437176444108 27.754541292381983 22.758000654785047 78-79 22.389857855828165 27.269101672500724 27.735399799472617 22.605640672198493 80-81 22.39823453999323 27.49730822351706 27.54984863054372 22.55460860594599 82-83 22.38249084401332 27.438609728765385 27.60195476918601 22.576944658035288 84-85 22.310220175355017 27.410939641219876 27.45918752985518 22.81965265356993 86-87 22.423850803944056 27.357342254215073 27.513690021527392 22.705116920313476 88-89 22.34232177621581 27.379852753619854 27.329783740871438 22.948041729292896 90-91 22.320244949170363 27.352785254448754 27.482057588378726 22.844912208002157 92-93 22.45931732597651 27.222514794062995 27.61788646741725 22.70028141254324 94-95 22.192551411553648 27.0699147251949 27.63941524203612 23.09811862121533 96-97 22.203120668779597 27.001589333084375 27.719331002993545 23.07595899514248 98-99 22.103783084763574 27.235696832618256 27.354430877631025 23.30608920498715 100-101 22.098333369582043 27.114280669927343 27.523390013522903 23.26399594696771 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 265.0 1 240.0 2 673.0 3 968.0 4 614.0 5 498.5 6 789.5 7 1496.0 8 2359.0 9 2711.0 10 2382.5 11 1989.5 12 1957.5 13 2079.5 14 2263.0 15 2465.0 16 2573.0 17 2730.0 18 2920.0 19 3019.5 20 3317.5 21 3812.0 22 4414.0 23 5215.0 24 6129.5 25 7297.5 26 9078.0 27 11153.5 28 12951.0 29 15183.0 30 18299.0 31 21910.5 32 26085.5 33 28872.0 34 31014.5 35 33211.5 36 35604.5 37 38723.0 38 40577.5 39 41201.5 40 41963.0 41 44449.5 42 46883.0 43 48753.5 44 50702.0 45 53396.5 46 54945.0 47 56613.5 48 63113.0 49 63027.5 50 56684.5 51 53504.5 52 51449.5 53 51132.0 54 50926.0 55 48464.5 56 44741.0 57 40570.5 58 37373.0 59 33616.0 60 28263.5 61 23733.0 62 20372.0 63 16296.5 64 12211.5 65 9648.5 66 7395.5 67 5856.5 68 4451.0 69 3042.5 70 2317.0 71 1892.5 72 1620.0 73 1459.5 74 1172.0 75 923.0 76 701.0 77 433.0 78 276.5 79 191.0 80 129.5 81 85.5 82 68.0 83 42.0 84 31.0 85 25.5 86 15.5 87 12.0 88 12.5 89 10.5 90 5.0 91 1.5 92 2.0 93 2.0 94 2.5 95 1.5 96 1.0 97 1.0 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012340737349465385 2 0.00639416442977481 3 0.009271538423173475 4 0.03484819614227272 5 0.046485575404462874 6 0.05486193080746787 7 0.02455359141033527 8 0.021995925638425347 9 0.01387533681261134 10-11 0.04332046401172434 12-13 0.04121038974989865 14-15 0.01758395218188073 16-17 0.021196655084703496 18-19 0.028134323491009163 20-21 0.023498554279422428 22-23 0.013171978725336108 24-25 0.002493724127612176 26-27 0.0020781034396768136 28-29 0.0011829204195083398 30-31 0.005722777164648455 32-33 0.0013747453524015842 34-35 0.0015985411074437026 36-37 0.006106427030434945 38-39 0.005946572919690574 40-41 0.003932411124311508 42-43 7.033580872752291E-4 44-45 9.910954866150957E-4 46-47 0.0029093448155475387 48-49 5.435039765308589E-4 50-51 0.0013108037081038361 52-53 7.992705537218513E-4 54-55 0.002493724127612176 56-57 0.001918249328932443 58-59 0.0020781034396768136 60-61 5.754747986797329E-4 62-63 0.0013747453524015842 64-65 0.003101169748440783 66-67 0.002493724127612176 68-69 0.006010514563988322 70-71 0.010262633909788571 72-73 0.0037086153692693895 74-75 0.0033569363256317755 76-77 0.0034528487920783973 78-79 0.03507199189731483 80-81 0.023498554279422428 82-83 0.0037405861914182642 84-85 0.008120588825814009 86-87 0.006681901829114677 88-89 0.005403068943159715 90-91 0.011061904463510422 92-93 0.01326789119178273 94-95 0.006362193607625936 96-97 0.003996352768609257 98-99 0.022219721393467465 100-101 0.04168995208213176 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1563926.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.52569341270366 #Duplication Level Percentage of deduplicated Percentage of total 1 82.17484389589062 50.55864251775349 2 10.775478049288491 13.259375176716837 3 3.016241773748711 5.56729099890758 4 1.2899052474937884 3.1744925915496194 5 0.6693614240511859 2.0591462879231996 6 0.4254938051556611 1.5707280843007125 7 0.2773766826764317 1.1946054916728048 8 0.21373858744627097 1.0520331841346895 9 0.14802733566633505 0.8196736023815675 >10 0.8976767516334697 10.357449076957021 >50 0.07175994525126957 3.08428283613836 >100 0.0360349491942528 4.175453509319124 >500 0.0031242711565871895 1.33697468682547 >1k 7.289966032036775E-4 0.8771133177395694 >5k 2.0828474377247927E-4 0.9127386376799012 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8011 0.51223651246926 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6234 0.39861221055216167 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3867 0.24726233849939194 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2572 0.16445790913380812 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1729 0.11055510299080647 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1701 0.10876473695046952 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1632 0.1043527634939249 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 6.39416442977481E-5 0.0 22-23 0.0 0.0 0.0 6.39416442977481E-5 0.0 24-25 0.0 0.0 0.0 1.278832885954962E-4 0.0 26-27 0.0 0.0 0.0 1.9182493289324431E-4 0.0 28-29 0.0 0.0 0.0 4.475915100842367E-4 0.0 30-31 0.0 0.0 0.0 0.0011189787752105918 3.197082214887405E-5 32-33 0.0 0.0 0.0 0.0022379575504211835 6.39416442977481E-5 34-35 0.0 0.0 0.0 0.0040602944129070045 6.39416442977481E-5 36-37 0.0 0.0 0.0 0.00639416442977481 6.39416442977481E-5 38-39 0.0 0.0 0.0 0.00876000526879149 6.39416442977481E-5 40-41 0.0 0.0 0.0 0.011957087483678895 6.39416442977481E-5 42-43 0.0 0.0 0.0 0.01752001053758298 6.39416442977481E-5 44-45 0.0 0.0 0.0 0.0261201616956301 6.39416442977481E-5 46-47 0.0 0.0 0.0 0.04204163112576938 6.39416442977481E-5 48-49 0.0 0.0 0.0 0.06400558594204586 6.39416442977481E-5 50-51 0.0 0.0 0.0 0.08894282721816761 6.39416442977481E-5 52-53 0.0 0.0 0.0 0.11362430191709838 6.39416442977481E-5 54-55 0.0 0.0 0.0 0.14444417446861296 6.39416442977481E-5 56-57 0.0 0.0 0.0 0.17414506824491696 6.39416442977481E-5 58-59 0.0 0.0 0.0 0.21254202564571467 6.39416442977481E-5 60-61 0.0 0.0 0.0 0.2632477495738289 6.39416442977481E-5 62-63 0.0 0.0 0.0 0.3117794575958197 6.39416442977481E-5 64-65 0.0 0.0 0.0 0.36638562182609663 6.39416442977481E-5 66-67 0.0 0.0 0.0 0.44631267719828177 6.39416442977481E-5 68-69 0.0 0.0 0.0 0.5347439712620674 6.39416442977481E-5 70-71 0.0 0.0 0.0 0.6272995013830578 6.39416442977481E-5 72-73 0.0 0.0 0.0 0.7250982463364635 1.9182493289324431E-4 74-75 0.0 0.0 0.0 0.8219378666254029 1.9182493289324431E-4 76-77 0.0 0.0 0.0 0.9190971951358313 1.9182493289324431E-4 78-79 0.0 0.0 0.0 1.0391156614827044 1.9182493289324431E-4 80-81 0.0 0.0 0.0 1.1915205706663872 1.9182493289324431E-4 82-83 0.0 0.0 0.0 1.3637473895823715 1.9182493289324431E-4 84-85 0.0 0.0 0.0 1.5409936275757294 1.9182493289324431E-4 86-87 0.0 0.0 0.0 1.7715032552691112 1.9182493289324431E-4 88-89 0.0 0.0 0.0 2.0356781586852577 1.9182493289324431E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1885 0.0 36.050816 2 GTACCTG 2090 0.0 34.334835 1 GTATCAA 11295 0.0 29.241716 1 GGTATCA 7765 0.0 28.09154 1 GTACATG 8315 0.0 26.462023 1 TACATGG 8325 0.0 25.63023 2 ACATGGG 8135 0.0 24.826862 3 ATCAACG 13320 0.0 24.474302 3 TCAACGC 13340 0.0 24.437609 4 TATCAAC 13525 0.0 24.243885 2 CAACGCA 13560 0.0 24.14781 5 AACGCAG 13835 0.0 23.770115 6 GAGTACT 7235 0.0 22.687613 12-13 CATGGGG 3385 0.0 22.181395 4 GTACACG 345 0.0 22.039675 1 TATCACG 110 0.0028702675 21.600697 2 ACGCAGA 15135 0.0 21.567276 7 CGCAGAG 15325 0.0 21.36189 8 ACCTGGG 3070 0.0 21.206676 3 AGTACTT 7495 0.0 20.759598 12-13 >>END_MODULE