FastQCFastQC Report
Thu 26 May 2016
SRR936394_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936394_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563926
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74540.47662101659541434No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65170.41670769588842443No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44190.2825581261517489No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30460.1947662485309407No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19030.12168094909861464No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16350.10454458842681816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1208.731149E-1139.5810932
GTACCTG17150.036.9296761
TACCTGG16500.036.84642
TATAACG1658.418829E-825.9076272
ATCACGC1701.12502676E-725.1432253
GTATCAA118750.024.9297121
GGTATCA88700.024.8287451
TAAGGTG8800.022.6655485
GTACATG81350.022.6484971
GTACACG3750.021.7512051
TACATGG83950.021.7259882
ACATGGG82450.021.4279023
AGTACTT83950.020.6759912-13
GTAAGGT8050.020.6490254
GAGTACT81650.020.53138212-13
GTAGGAC3052.0918378E-1020.2428133
ACCTGGG29300.020.0993253
TCACGCA2601.4937541E-820.0930924
ATCAACG147800.020.051073
ATTCGCG3200.020.0385780-81
Produced by FastQC (version 0.11.5)