##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936394_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1563926 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.30121374029206 31.0 31.0 34.0 30.0 34.0 2 31.509999833751724 31.0 31.0 34.0 30.0 34.0 3 31.776300796840772 31.0 31.0 34.0 30.0 34.0 4 35.62670676234042 37.0 35.0 37.0 33.0 37.0 5 35.42036899444091 37.0 35.0 37.0 33.0 37.0 6 35.53488783996174 37.0 35.0 37.0 33.0 37.0 7 35.4635698875778 37.0 35.0 37.0 33.0 37.0 8 35.44152152979105 37.0 35.0 37.0 33.0 37.0 9 37.03146696199181 39.0 37.0 39.0 33.0 39.0 10-11 36.90013498081112 39.0 37.0 39.0 33.0 39.0 12-13 36.76344117304782 39.0 37.0 39.0 32.0 39.0 14-15 37.807103724856546 39.5 37.0 41.0 33.0 41.0 16-17 37.712962122248754 39.0 37.0 41.0 32.0 41.0 18-19 37.77617067559463 39.5 37.0 41.0 32.5 41.0 20-21 37.85917556201508 40.0 37.0 41.0 33.0 41.0 22-23 37.75831752909025 39.5 37.0 41.0 32.0 41.0 24-25 37.633357972180264 39.0 36.5 41.0 32.0 41.0 26-27 37.39249075723532 39.0 36.0 41.0 32.0 41.0 28-29 37.286808966664665 39.0 36.0 41.0 31.5 41.0 30-31 37.23302509198005 39.0 36.0 41.0 31.5 41.0 32-33 37.0167421604347 39.0 35.5 41.0 31.0 41.0 34-35 36.807903634826715 39.0 35.0 40.5 30.5 41.0 36-37 36.547632049086715 39.0 35.0 40.0 30.0 41.0 38-39 36.36947400324568 39.0 35.0 40.0 30.0 41.0 40-41 36.10052361812515 38.0 35.0 40.0 30.0 41.0 42-43 35.81589921773792 38.0 35.0 40.0 29.0 41.0 44-45 35.49548412137147 38.0 35.0 40.0 27.5 41.0 46-47 35.04016142707519 38.0 34.0 40.0 26.5 41.0 48-49 34.53599179245054 38.0 33.5 40.0 24.0 41.0 50-51 34.30605923809694 38.0 33.0 40.0 24.0 41.0 52-53 33.997317648021706 38.0 33.0 40.0 23.0 41.0 54-55 33.692563458884884 37.0 33.0 40.0 22.5 41.0 56-57 33.389319571386366 37.0 32.5 40.0 22.0 41.0 58-59 32.98671836135469 36.5 32.0 39.5 20.5 41.0 60-61 32.59469118104053 36.0 31.0 39.0 19.0 40.0 62-63 32.27716720612101 35.5 31.0 39.0 18.0 40.0 64-65 31.80805549623192 35.0 31.0 38.5 16.5 40.0 66-67 31.37282454540688 35.0 30.0 38.0 13.0 40.0 68-69 30.901876751201783 34.5 29.5 37.5 10.0 39.5 70-71 30.40810178998239 34.0 29.0 37.0 8.0 39.0 72-73 29.96279203747492 34.0 29.0 36.0 7.0 39.0 74-75 29.563189370852584 34.0 28.5 36.0 6.5 38.5 76-77 28.233620388688465 32.0 26.5 34.5 2.0 36.5 78-79 29.032946571640856 33.5 28.5 35.0 2.0 37.0 80-81 28.869670304093674 34.0 28.5 35.0 2.0 37.0 82-83 28.504909439449182 33.5 27.5 35.0 2.0 36.0 84-85 28.006728579229453 33.0 27.0 35.0 2.0 36.0 86-87 27.569659625839073 33.0 26.0 35.0 2.0 35.5 88-89 27.0963718871609 32.5 25.5 35.0 2.0 35.0 90-91 26.576330337880435 32.0 25.0 34.0 2.0 35.0 92-93 26.029076183911513 32.0 24.0 34.0 2.0 35.0 94-95 25.402231307619417 32.0 21.5 34.0 2.0 35.0 96-97 24.600910145364935 31.0 18.0 34.0 2.0 35.0 98-99 23.584175338219328 31.0 2.0 34.0 2.0 35.0 100-101 20.699590645593204 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4327762313040857 1101 2 0.47617011588928193 1101 3 0.21773745918333276 1101 4 0.14166132801150866 1101 5 0.29374765707539296 1101 6 0.19499724145585162 1101 7 0.21192581198324945 1101 8 0.15878242152652433 1101 9 0.3056305587585655 1101 10-11 0.26171720076510496 1101 12-13 0.22416355193412585 1101 14-15 0.34584551435712996 1101 16-17 0.2759790492279137 1101 18-19 0.3246482781520186 1101 20-21 0.19583857207565813 1101 22-23 0.07809291133937535 1101 24-25 -0.001317709625858754 1101 26-27 0.14197894930883592 1101 28-29 0.13959209901180003 1101 30-31 -0.026455681496102557 1101 32-33 0.15722836267587326 1101 34-35 0.06590632565677623 1101 36-37 0.18173018818249886 1101 38-39 0.1603889769814444 1101 40-41 0.39708003402785863 1101 42-43 0.5666043891332748 1101 44-45 0.46940789672066785 1101 46-47 0.39686706880978306 1101 48-49 0.32423557039812323 1101 50-51 0.33065199616213903 1101 52-53 0.3905437414184263 1101 54-55 0.34636269472278514 1101 56-57 0.24374944106289576 1101 58-59 0.2674571201280358 1101 60-61 0.3646301144496462 1101 62-63 0.07607756262427756 1101 64-65 0.34361817493348923 1101 66-67 0.16171730706086151 1101 68-69 0.14186136262384252 1101 70-71 0.05381093856136587 1101 72-73 0.06879089403829042 1101 74-75 0.13423575729222037 1101 76-77 0.441643874080647 1101 78-79 0.5635234729281358 1101 80-81 0.32164249168933523 1101 82-83 0.4901008681322736 1101 84-85 0.4362229121703969 1101 86-87 0.410633499325165 1101 88-89 0.3799574233395724 1101 90-91 0.2850873000540517 1101 92-93 0.45773084468858727 1101 94-95 0.052045826903547976 1101 96-97 0.28658132820706683 1101 98-99 0.3218834037681866 1101 100-101 0.0015646213829114686 1102 1 0.4349238924676939 1102 2 0.4449888100477075 1102 3 0.23254376271986743 1102 4 0.11086758678258235 1102 5 0.1260072128163401 1102 6 0.023773484730696737 1102 7 0.012855267593273823 1102 8 -0.13776173572233574 1102 9 -0.02049663746739583 1102 10-11 0.13223691886987154 1102 12-13 0.05560489805190372 1102 14-15 0.3125678940992316 1102 16-17 0.37038632336104627 1102 18-19 0.13274895084209248 1102 20-21 0.20696555528416383 1102 22-23 0.04385957391525608 1102 24-25 -0.07840332547842621 1102 26-27 -0.2869538141126995 1102 28-29 -0.08460782964323954 1102 30-31 0.08093929930371502 1102 32-33 0.2327150757909493 1102 34-35 0.1816657392801062 1102 36-37 0.23332731047550936 1102 38-39 0.019206246784285952 1102 40-41 0.3002998407097408 1102 42-43 0.13746814634168913 1102 44-45 0.09922169402088343 1102 46-47 0.14831447479754445 1102 48-49 0.3245830750097909 1102 50-51 0.20588862041395828 1102 52-53 0.15130907184030207 1102 54-55 0.3318825940513648 1102 56-57 0.21886884212137403 1102 58-59 0.30172415882281456 1102 60-61 0.30153624878036567 1102 62-63 0.19231706038632268 1102 64-65 0.09499007099877588 1102 66-67 0.1485817599143715 1102 68-69 -0.044108925572889035 1102 70-71 -0.085302501823314 1102 72-73 -0.017526326484382793 1102 74-75 -0.5618029444839685 1102 76-77 -0.05859060409968819 1102 78-79 -0.014993334149895787 1102 80-81 -0.19015040955131823 1102 82-83 0.016178657023697696 1102 84-85 -0.006512429207578663 1102 86-87 0.023118643741469214 1102 88-89 -0.08406217808249394 1102 90-91 -0.1139632461290887 1102 92-93 0.19902669669804496 1102 94-95 0.021522808617746136 1102 96-97 -0.10698513101662144 1102 98-99 -0.5121542119368598 1102 100-101 -0.37891239148906664 1103 1 0.3652914059176524 1103 2 0.41844263736906484 1103 3 0.2550473128982631 1103 4 0.16866932649724475 1103 5 -0.02147088805453734 1103 6 0.05117093674603268 1103 7 0.10992401635262183 1103 8 0.10198601074148428 1103 9 0.060334816858727436 1103 10-11 0.05053796127351262 1103 12-13 0.15655402997602152 1103 14-15 0.16266097494150245 1103 16-17 0.12465576281459789 1103 18-19 0.056875339738049036 1103 20-21 0.11129333727521384 1103 22-23 0.06328439411590381 1103 24-25 0.093205808787026 1103 26-27 0.04328880686058767 1103 28-29 0.14038568078334634 1103 30-31 0.22788391906561145 1103 32-33 0.18756927670813894 1103 34-35 0.13765593366191098 1103 36-37 0.16600371235061573 1103 38-39 0.18725693162515 1103 40-41 0.21322980676036707 1103 42-43 0.14315065859553044 1103 44-45 0.3174905430504964 1103 46-47 0.22117743282146307 1103 48-49 0.4047334422432982 1103 50-51 0.1836452894049998 1103 52-53 0.4040152875791776 1103 54-55 0.3084507803810226 1103 56-57 0.192042408943621 1103 58-59 0.200279719751002 1103 60-61 0.31310804891168686 1103 62-63 0.3344565327712772 1103 64-65 0.3094653826255467 1103 66-67 0.17471724439283776 1103 68-69 0.28214260774788613 1103 70-71 0.28171086924286115 1103 72-73 0.35018053821182704 1103 74-75 0.37782907125388476 1103 76-77 0.34586187949823355 1103 78-79 0.39218127100409816 1103 80-81 0.49679129626486684 1103 82-83 0.6187543178149681 1103 84-85 0.6488412779930464 1103 86-87 0.6272311245908639 1103 88-89 0.6109797242023447 1103 90-91 0.3141227369013109 1103 92-93 0.2860350160766707 1103 94-95 0.33254293138520197 1103 96-97 -0.15439019237490115 1103 98-99 -0.2358768475689459 1103 100-101 -3.214941973794794E-4 1104 1 0.29628688103077394 1104 2 0.4970625468713301 1104 3 0.2369477653869545 1104 4 0.10588652106738294 1104 5 0.11484132914003453 1104 6 0.23951935303562522 1104 7 0.02960727427117149 1104 8 0.095198680424744 1104 9 0.1774162648225328 1104 10-11 0.16507416036852618 1104 12-13 0.10904271775882535 1104 14-15 0.24495735503199967 1104 16-17 0.1368163962987552 1104 18-19 0.1329500003715367 1104 20-21 -0.019928382181795712 1104 22-23 0.05706267465888715 1104 24-25 0.10338680426213642 1104 26-27 0.2313544177203184 1104 28-29 0.28518206087383646 1104 30-31 0.22731830820588073 1104 32-33 0.07868918621040422 1104 34-35 0.29546136352617225 1104 36-37 0.26272316936418605 1104 38-39 0.1895193750640587 1104 40-41 0.163738856534124 1104 42-43 0.27069590746430805 1104 44-45 0.507535791919274 1104 46-47 0.3563584282965735 1104 48-49 0.45167914360076367 1104 50-51 0.5945615789977552 1104 52-53 0.3290718486651514 1104 54-55 0.28808878943079463 1104 56-57 0.4230944451427163 1104 58-59 0.325279719751002 1104 60-61 0.1456872344320459 1104 62-63 0.14328006218304523 1104 64-65 0.17880927402826075 1104 66-67 0.47222855661003393 1104 68-69 0.4246765443994711 1104 70-71 0.0871407334962555 1104 72-73 0.3654520314244998 1104 74-75 0.23529513460229978 1104 76-77 0.4360768116249325 1104 78-79 0.4674075153479933 1104 80-81 0.5262030609707473 1104 82-83 0.36366382007741294 1104 84-85 0.5818163911152183 1104 86-87 0.5678419843193723 1104 88-89 0.5991018961480457 1104 90-91 0.3585231893899987 1104 92-93 0.336939993452237 1104 94-95 0.16314247889651767 1104 96-97 -0.07435625572331617 1104 98-99 0.23782224745368197 1104 100-101 0.3619522614587254 1105 1 0.3261944280951852 1105 2 0.3368931048391417 1105 3 0.15851766244704635 1105 4 0.01778263149289927 1105 5 -0.0979565846382755 1105 6 0.009084664267604126 1105 7 -0.0179230820827172 1105 8 -0.06713861916745856 1105 9 0.007020756191728594 1105 10-11 -0.10879703016932041 1105 12-13 -0.012684229948511927 1105 14-15 0.0438328622408406 1105 16-17 0.11970206545893802 1105 18-19 0.07239980256643719 1105 20-21 9.382783907483372E-4 1105 22-23 -0.014298244741247856 1105 24-25 0.04327906523283076 1105 26-27 0.15280624113292873 1105 28-29 0.16527128900228405 1105 30-31 0.012690570293827363 1105 32-33 0.20304454218340595 1105 34-35 0.15059039390029483 1105 36-37 0.06610578892328078 1105 38-39 0.06328848902925444 1105 40-41 0.0786813940256792 1105 42-43 0.19969111342029322 1105 44-45 0.02028806324997845 1105 46-47 0.4061547195144328 1105 48-49 0.47481745227829464 1105 50-51 0.2991156034047222 1105 52-53 0.33940456242923744 1105 54-55 0.3858505955259375 1105 56-57 0.38933553883479277 1105 58-59 0.6153838150021542 1105 60-61 0.47921100913229253 1105 62-63 0.45874745608180234 1105 64-65 0.362399195363281 1105 66-67 0.20869230165220998 1105 68-69 0.1866289619891397 1105 70-71 0.06584684945727304 1105 72-73 -0.17753883754675925 1105 74-75 0.08667754848471532 1105 76-77 0.08415417906504175 1105 78-79 0.10399999875419752 1105 80-81 -0.19077059717938027 1105 82-83 0.04539217705086429 1105 84-85 0.24131801875410375 1105 86-87 0.27383225403513123 1105 88-89 0.0862893183844875 1105 90-91 -0.15927323894564438 1105 92-93 -0.08701835575120853 1105 94-95 -0.09803579453665989 1105 96-97 -0.04967600677836259 1105 98-99 0.021132346940252233 1105 100-101 0.21416309528720845 1106 1 0.3195193552535436 1106 2 0.4015950709644578 1106 3 0.1650528482474769 1106 4 0.10704222104101291 1106 5 0.0317569124535666 1106 6 0.08636674972433411 1106 7 0.10736130632919583 1106 8 0.09647546616233882 1106 9 0.13737702128544527 1106 10-11 0.014379500152806202 1106 12-13 0.011981870693738017 1106 14-15 0.06839048566363459 1106 16-17 0.09627649086968404 1106 18-19 0.0870764465467957 1106 20-21 0.11266770965278283 1106 22-23 -0.009346502532345369 1106 24-25 0.0816210670293458 1106 26-27 0.18529678742419975 1106 28-29 0.13440450097065337 1106 30-31 -0.04162769733468963 1106 32-33 0.052288083579284717 1106 34-35 -0.19594073129018597 1106 36-37 0.14020754897426002 1106 38-39 0.10244989859842946 1106 40-41 0.2449165804089759 1106 42-43 0.0787318898451943 1106 44-45 -0.0587142766198383 1106 46-47 0.05942104954552008 1106 48-49 -0.14170294823903617 1106 50-51 -0.032407941332898815 1106 52-53 -0.012262392430642421 1106 54-55 -0.22144891752468254 1106 56-57 -0.10567305191336374 1106 58-59 -0.20323369302677463 1106 60-61 0.01594204326666926 1106 62-63 -0.3530599492532005 1106 64-65 -0.20904017752829418 1106 66-67 -0.14156023979094456 1106 68-69 -0.013002636421067137 1106 70-71 -0.17332420972724805 1106 72-73 -0.13496670991019855 1106 74-75 0.07810606717809421 1106 76-77 -0.09057331644529754 1106 78-79 -0.06145544689935889 1106 80-81 -0.21536205901839267 1106 82-83 -0.07277578195186862 1106 84-85 -0.06681589459235227 1106 86-87 0.026332087271644156 1106 88-89 -0.044966174286660276 1106 90-91 -0.1061143823149635 1106 92-93 0.28629628064286905 1106 94-95 0.3137767594186158 1106 96-97 0.2857415463252835 1106 98-99 0.5173107106892978 1106 100-101 0.5580865576469094 1107 1 0.48678352737667296 1107 2 0.5049116251620553 1107 3 0.3317295960019564 1107 4 0.2223881724328507 1107 5 0.09402612202824656 1107 6 0.10369818063888658 1107 7 0.04499116218200783 1107 8 0.06551193063206995 1107 9 0.3267659032680683 1107 10-11 0.26905635181052645 1107 12-13 0.3629430684011865 1107 14-15 0.39135235874211816 1107 16-17 0.36442402604370727 1107 18-19 0.20893972844184105 1107 20-21 0.1917493905844907 1107 22-23 0.43768641067320146 1107 24-25 0.229333247642856 1107 26-27 0.33901253414521904 1107 28-29 0.38219869092431935 1107 30-31 0.5043459594840556 1107 32-33 0.39809363980485557 1107 34-35 0.3505414947624388 1107 36-37 0.5131491052591954 1107 38-39 0.4163660672408085 1107 40-41 0.7053750905478822 1107 42-43 0.5530572145891881 1107 44-45 0.27858179008603656 1107 46-47 0.5560148788065291 1107 48-49 0.5551235500549083 1107 50-51 0.477416295210233 1107 52-53 0.22912022567115997 1107 54-55 0.26755329598532995 1107 56-57 0.6450619707134209 1107 58-59 0.22552542401836462 1107 60-61 0.2726837862063505 1107 62-63 0.2858525549446753 1107 64-65 0.24794147056257998 1107 66-67 0.09101138386411023 1107 68-69 0.2175460283295756 1107 70-71 0.013550213934486521 1107 72-73 0.24071028461447241 1107 74-75 0.550967283996318 1107 76-77 0.4327633504502053 1107 78-79 0.43351396658542285 1107 80-81 0.4746331725652233 1107 82-83 0.2578114754294347 1107 84-85 0.3906177325768603 1107 86-87 0.6798583961277238 1107 88-89 0.7196677616235974 1107 90-91 0.5906278801409002 1107 92-93 1.0037371810386269 1107 94-95 1.0624255534916784 1107 96-97 1.0689242508894168 1107 98-99 1.2606572855005602 1107 100-101 1.447149443225328 1108 1 0.35047401838629 1108 2 0.3733729853730239 1108 3 0.04664986128005921 1108 4 -0.05160090007049689 1108 5 -0.04786931795382543 1108 6 -0.033496865409425425 1108 7 -0.06772023968213858 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-1.6539569464286217 2316 80-81 -1.465210445221203 2316 82-83 -1.33350738139913 2316 84-85 -1.7218143096468665 2316 86-87 -1.517931786221233 2316 88-89 -1.9021744494104205 2316 90-91 -1.5203709669567473 2316 92-93 -1.6830573273384282 2316 94-95 -1.9955355545638334 2316 96-97 -2.071247256556852 2316 98-99 -2.3532224953422265 2316 100-101 -2.785493558974707 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 13.0 4 27.0 5 73.0 6 176.0 7 425.0 8 998.0 9 1812.0 10 2928.0 11 4617.0 12 6567.0 13 8544.0 14 10236.0 15 13100.0 16 16502.0 17 20032.0 18 17266.0 19 12373.0 20 11614.0 21 12247.0 22 12954.0 23 15003.0 24 17166.0 25 20681.0 26 25189.0 27 30976.0 28 37483.0 29 45966.0 30 56250.0 31 67463.0 32 82264.0 33 102141.0 34 130120.0 35 168858.0 36 222020.0 37 255709.0 38 128606.0 39 5521.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.511640705134 19.401390528798828 14.824168641017756 22.262800125049413 2 16.136924787801625 21.20685608751788 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25.517287902368786 30.385772728377177 21.43336705189376 22-23 22.175231647406143 25.64171015284157 30.3282100534111 21.854848146341183 24-25 22.266047114761182 25.86685687174457 30.401886022740204 21.46520999075404 26-27 21.94534779778583 26.006825131112343 30.376916810641934 21.670910260459895 28-29 21.655244557606945 26.388364922637003 30.41940603327779 21.53698448647826 30-31 21.827311522412185 25.914205659347054 30.389353460457848 21.869129357782914 32-33 21.62086034170425 26.15564494934672 30.307468601869598 21.91602610707944 34-35 22.397039236636633 26.074622695136135 29.925776301999907 21.60256176622732 36-37 22.446278973034858 25.797634679432274 29.913270726608243 21.842815620924625 38-39 22.014489829911867 26.090006263090064 30.032683802830633 21.862820104167433 40-41 22.263249334503932 26.01781093197585 29.979199051584178 21.73974068193604 42-43 22.083017588959976 26.26612694362644 29.4401652422199 22.210690225193684 44-45 22.281717320981315 26.321774843804054 29.341019668124773 22.05548816708986 46-47 22.589497066959268 26.684724017939697 28.654215790463113 22.071563124637926 48-49 22.79686503817985 26.81634855987052 28.24739749696729 22.13938890498234 50-51 22.52521470907851 27.022539857415843 28.242040745729398 22.21020468777625 52-53 22.913797226666258 27.091542009880463 27.949208833846797 22.045451929606482 54-55 22.454355178771156 26.9686377504462 28.138191370920968 22.43881569986168 56-57 22.68582980496147 27.261176676713323 27.82541776956596 22.22757574875925 58-59 22.906489853479467 26.876697867301232 27.879312786761133 22.337499492458168 60-61 22.55063705977331 26.969577798027274 28.371748757054014 22.108036385145397 62-63 22.562393293560206 26.99329228132773 28.322526808494313 22.12178761661775 64-65 22.227615556721624 27.170190933634164 28.223745785924486 22.378447723719724 66-67 22.291845707621004 27.29169224394586 27.894772515678874 22.521689532754262 68-69 22.42826048869445 27.08044263077059 28.06396354805419 22.427333332480774 70-71 22.489551019129316 27.09467977186645 27.861380637038547 22.554388571965685 72-73 22.568530278456358 27.092850650494544 27.85100696932175 22.487612101727354 74-75 22.377657254882905 27.613614710670454 27.46785973249374 22.540868301952905 76-77 22.463395350041708 27.34245941173593 27.605326789037584 22.58881844918478 78-79 22.531770227991053 27.392211438081425 27.60845202371836 22.467566310209158 80-81 22.56563115327035 27.652089497928312 27.363191556442057 22.419087792359278 82-83 22.560914780059626 27.569125853241776 27.404872415014754 22.465086951683848 84-85 22.480946331915106 27.573296312555296 27.263443399167354 22.68231395636225 86-87 22.585044193148505 27.495035205199265 27.21430167854988 22.705618923102357 88-89 22.45699133111015 27.49465629521043 27.150689277157596 22.89766309652183 90-91 22.474008431910278 27.502455896197453 27.23355444926528 22.78998122262699 92-93 22.570840203224076 27.433300114873845 27.322134971812268 22.67372471008981 94-95 22.273706679490175 27.33648826620122 27.351100160538998 23.03870489376961 96-97 22.274144597081662 27.181624360678004 27.446209982823515 23.09802105941682 98-99 22.183228288450522 27.312415588933455 27.042715836539248 23.46164028607678 100-101 22.085290296712365 27.397220853542454 27.138196653117003 23.379292196628178 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 532.0 1 412.5 2 656.0 3 926.0 4 677.5 5 543.5 6 790.5 7 1408.0 8 2038.5 9 2345.0 10 2199.5 11 1931.0 12 1937.0 13 2068.0 14 2309.0 15 2529.0 16 2634.0 17 2860.0 18 3114.5 19 3338.0 20 3677.5 21 4188.5 22 4752.5 23 5542.5 24 6573.0 25 7741.0 26 9480.0 27 11635.0 28 13570.0 29 15769.0 30 18886.0 31 22363.0 32 26302.0 33 29006.0 34 31219.0 35 33518.5 36 36228.0 37 39401.0 38 40875.0 39 41074.5 40 41839.5 41 44485.5 42 46837.0 43 48599.5 44 50439.5 45 52867.5 46 54188.5 47 56286.5 48 63203.0 49 63154.5 50 56721.0 51 53401.0 52 51282.0 53 51058.5 54 50647.0 55 47861.0 56 44073.5 57 39967.5 58 36915.0 59 33304.0 60 28038.5 61 23535.5 62 20048.5 63 15913.5 64 12065.5 65 9456.0 66 7052.5 67 5738.0 68 4433.0 69 2973.0 70 2184.5 71 1726.5 72 1522.5 73 1349.0 74 1097.0 75 899.0 76 650.5 77 395.0 78 273.5 79 207.0 80 137.0 81 104.5 82 74.0 83 48.5 84 37.0 85 21.5 86 12.5 87 6.0 88 1.5 89 2.0 90 2.0 91 1.5 92 0.5 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0063135979579596 2 0.011317671040701414 3 0.0035167904363761454 4 0.01112584610780817 5 0.0 6 0.0 7 0.0 8 0.0 9 0.003005257281994161 10-11 1.278832885954962E-4 12-13 1.5985411074437026E-4 14-15 3.197082214887405E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0012148912416572138 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0037725570135671385 34-35 3.197082214887405E-4 36-37 0.002333870016867806 38-39 9.591246644662214E-5 40-41 0.0035167904363761454 42-43 0.010294604731937445 44-45 0.0015985411074437026 46-47 0.001278832885954962 48-49 0.00914365513457798 50-51 4.7956233223311076E-4 52-53 0.0020461326175279394 54-55 0.010678254597723933 56-57 0.00354876125852502 58-59 9.910954866150957E-4 60-61 0.006458106074072559 62-63 0.003580732080673894 64-65 0.017104389849647617 66-67 0.0024297824833144277 68-69 2.557665771909924E-4 70-71 0.0011189787752105918 72-73 3.197082214887405E-5 74-75 0.0 76-77 0.001246862063806088 78-79 0.010134750621193074 80-81 0.014386869966993322 82-83 0.01176526255078565 84-85 0.007641026493580898 86-87 0.010582342131277312 88-89 0.025832424296290235 90-91 0.02186804234982985 92-93 0.002333870016867806 94-95 0.008408326225153875 96-97 0.010230663087639697 98-99 0.009591246644662215 100-101 0.011029933641361548 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1563926.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.68609680085629 #Duplication Level Percentage of deduplicated Percentage of total 1 81.42845543413385 49.415751288200596 2 11.327894102018664 13.748913560499076 3 3.1330543985965007 5.703985275467275 4 1.3026752863108328 3.162171141005696 5 0.6814801360627658 2.0678184752482864 6 0.42391786593665864 1.5435552388706693 7 0.2809078471410294 1.1933040562604458 8 0.20863876659798844 1.0129177908941431 9 0.15836797045445683 0.8649660586638885 >10 0.9363142043287873 10.57068299083989 >50 0.07460427905065395 3.0975257128750235 >100 0.03935268855465812 4.338718697121199 >500 0.003279210858766651 1.3808848197105161 >1k 8.46247963552684E-4 1.0019455286152077 >5k 2.11561990888171E-4 0.8968593657280285 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7454 0.47662101659541434 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6517 0.41670769588842443 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4419 0.2825581261517489 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3046 0.1947662485309407 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1903 0.12168094909861464 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1635 0.10454458842681816 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.39416442977481E-5 2 0.0 0.0 0.0 0.0 6.39416442977481E-5 3 0.0 0.0 0.0 0.0 6.39416442977481E-5 4 0.0 0.0 0.0 0.0 6.39416442977481E-5 5 0.0 0.0 0.0 0.0 6.39416442977481E-5 6 0.0 0.0 0.0 0.0 6.39416442977481E-5 7 0.0 0.0 0.0 0.0 6.39416442977481E-5 8 0.0 0.0 0.0 0.0 6.39416442977481E-5 9 0.0 0.0 0.0 0.0 6.39416442977481E-5 10-11 0.0 0.0 0.0 0.0 6.39416442977481E-5 12-13 0.0 0.0 0.0 0.0 6.39416442977481E-5 14-15 0.0 0.0 0.0 0.0 9.591246644662216E-5 16-17 0.0 0.0 0.0 0.0 1.278832885954962E-4 18-19 0.0 0.0 0.0 0.0 1.278832885954962E-4 20-21 0.0 0.0 0.0 6.39416442977481E-5 1.278832885954962E-4 22-23 0.0 0.0 0.0 6.39416442977481E-5 1.278832885954962E-4 24-25 0.0 0.0 0.0 1.278832885954962E-4 1.278832885954962E-4 26-27 0.0 0.0 0.0 1.278832885954962E-4 1.9182493289324431E-4 28-29 0.0 0.0 0.0 4.475915100842367E-4 1.9182493289324431E-4 30-31 0.0 0.0 0.0 0.0011509495973594658 1.9182493289324431E-4 32-33 0.0 0.0 0.0 0.0021420450839745617 2.557665771909924E-4 34-35 0.0 0.0 0.0 0.003964381946460383 3.197082214887405E-4 36-37 0.0 0.0 0.0 0.006330222785477062 3.197082214887405E-4 38-39 0.0 0.0 0.0 0.00876000526879149 3.197082214887405E-4 40-41 0.0 0.0 0.0 0.011829204195083399 3.197082214887405E-4 42-43 0.0 0.0 0.0 0.017232273138243114 3.197082214887405E-4 44-45 0.0 0.0 0.0 0.025864395118439108 3.197082214887405E-4 46-47 0.0 0.0 0.0 0.041945718659322756 3.197082214887405E-4 48-49 0.0 0.0 0.0 0.06368587772055712 3.197082214887405E-4 50-51 0.0 0.0 0.0 0.08852720653023224 3.197082214887405E-4 52-53 0.0 0.0 0.0 0.1125053231418878 3.516790436376146E-4 54-55 0.0 0.0 0.0 0.14316534158265798 3.8364986578648863E-4 56-57 0.0 0.0 0.0 0.17254652713747326 3.8364986578648863E-4 58-59 0.0 0.0 0.0 0.21036800973959124 4.1562068793536266E-4 60-61 0.0 0.0 0.0 0.26094585037911 4.4759151008423673E-4 62-63 0.0 0.0 0.0 0.3088701127802722 4.4759151008423673E-4 64-65 0.0 0.0 0.0 0.362485181523934 4.4759151008423673E-4 66-67 0.0 0.0 0.0 0.44174084963099275 4.4759151008423673E-4 68-69 0.0 0.0 0.0 0.5301401728726296 4.4759151008423673E-4 70-71 0.0 0.0 0.0 0.6228235862822153 4.4759151008423673E-4 72-73 0.0 0.0 0.0 0.7207821853463655 5.115331543819848E-4 74-75 0.0 0.0 0.0 0.8164708560379456 5.115331543819848E-4 76-77 0.0 0.0 0.0 0.913822009481267 5.115331543819848E-4 78-79 0.0 0.0 0.0 1.03412821322748 5.115331543819848E-4 80-81 0.0 0.0 0.0 1.1857977935017385 5.115331543819848E-4 82-83 0.0 0.0 0.0 1.356841691998215 5.754747986797329E-4 84-85 0.0 0.0 0.0 1.5318819432633002 5.754747986797329E-4 86-87 0.0 0.0 0.0 1.7587149264095616 5.754747986797329E-4 88-89 0.0 0.0 0.0 2.0187016521242054 5.754747986797329E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 120 8.731149E-11 39.581093 2 GTACCTG 1715 0.0 36.929676 1 TACCTGG 1650 0.0 36.8464 2 TATAACG 165 8.418829E-8 25.907627 2 ATCACGC 170 1.12502676E-7 25.143225 3 GTATCAA 11875 0.0 24.929712 1 GGTATCA 8870 0.0 24.828745 1 TAAGGTG 880 0.0 22.665548 5 GTACATG 8135 0.0 22.648497 1 GTACACG 375 0.0 21.751205 1 TACATGG 8395 0.0 21.725988 2 ACATGGG 8245 0.0 21.427902 3 AGTACTT 8395 0.0 20.67599 12-13 GTAAGGT 805 0.0 20.649025 4 GAGTACT 8165 0.0 20.531382 12-13 GTAGGAC 305 2.0918378E-10 20.242813 3 ACCTGGG 2930 0.0 20.099325 3 TCACGCA 260 1.4937541E-8 20.093092 4 ATCAACG 14780 0.0 20.05107 3 ATTCGCG 320 0.0 20.03857 80-81 >>END_MODULE