FastQCFastQC Report
Thu 26 May 2016
SRR936393_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936393_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1581630
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78630.4971453500502646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61400.38820710279901116No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38720.24481073323090735No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26380.16678995719605721No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17700.11190986513912862No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16860.10659888848845811No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16160.10217307461289935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG16800.040.7324032
GTACCTG19100.037.567881
TATAACG958.672432E-735.015572
GTATCAA111800.027.6276931
GGTATCA78850.027.4812341
GTACATG80350.026.849921
TACATGG82000.025.6730042
ACATGGG80600.024.8807953
ATCAACG131700.023.3816993
TCAACGC132150.023.3035534
TATCAAC131500.023.2365722
CAACGCA133050.023.2173585
AACGCAG136150.022.757816
GAGTACT71100.022.5272912-13
ATAACGC1754.0352406E-621.7239483
TAACGCA2005.3624535E-721.3858644
ACGCAGA148550.020.8601157
CATGGGA44800.020.7918134
ACCTGGG31550.020.785793
GTAGTAC3900.020.7143423
Produced by FastQC (version 0.11.5)