##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936393_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1581630 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.514143636628035 31.0 31.0 34.0 30.0 34.0 2 31.55654166903764 33.0 31.0 34.0 30.0 34.0 3 31.613370383718063 33.0 31.0 34.0 30.0 34.0 4 35.25082035621479 37.0 35.0 37.0 33.0 37.0 5 35.232642906368746 37.0 35.0 37.0 33.0 37.0 6 35.36332328041325 37.0 35.0 37.0 33.0 37.0 7 35.23993095730354 37.0 35.0 37.0 32.0 37.0 8 35.18967457622832 37.0 35.0 37.0 32.0 37.0 9 36.701961267806 39.0 37.0 39.0 32.0 39.0 10-11 36.60893413756694 39.0 37.0 39.0 32.0 39.0 12-13 36.50376130953509 39.0 37.0 39.0 32.0 39.0 14-15 37.57707712929067 40.0 37.0 41.0 32.0 41.0 16-17 37.45785297446305 40.0 37.0 41.0 32.0 41.0 18-19 37.41974134279193 40.0 37.0 41.0 32.0 41.0 20-21 37.333730392063885 40.0 37.0 41.0 31.5 41.0 22-23 37.196572839412504 39.0 36.5 41.0 31.0 41.0 24-25 37.31737606140501 39.5 37.0 41.0 32.0 41.0 26-27 37.28195690521804 40.0 37.0 41.0 31.5 41.0 28-29 37.17114400966092 39.0 36.5 41.0 31.0 41.0 30-31 36.95907513135184 39.0 36.0 41.0 31.0 41.0 32-33 36.74726326637709 39.0 35.5 41.0 30.0 41.0 34-35 36.50243988796368 39.0 35.0 41.0 30.0 41.0 36-37 36.29853948142107 39.0 35.0 40.0 30.0 41.0 38-39 36.084763503474264 39.0 35.0 40.0 30.0 41.0 40-41 35.87987329527134 38.0 35.0 40.0 29.0 41.0 42-43 35.62849180908304 38.0 35.0 40.0 27.5 41.0 44-45 35.33880205863571 38.0 35.0 40.0 26.5 41.0 46-47 35.003934864664934 38.0 34.0 40.0 25.5 41.0 48-49 34.845652270126386 38.0 34.0 40.0 24.0 41.0 50-51 33.916957505864204 37.5 33.0 39.5 23.5 40.5 52-53 34.04851197814912 38.0 33.5 39.5 23.5 40.5 54-55 34.45214051326796 38.0 34.0 40.0 24.0 41.0 56-57 34.367714952296055 38.0 34.0 40.0 23.0 41.0 58-59 34.104025909978944 38.0 34.0 40.0 23.0 41.0 60-61 33.78532747861384 37.0 33.0 40.0 22.0 41.0 62-63 33.45865815645884 37.0 33.0 40.0 21.5 41.0 64-65 33.119592761897536 36.0 33.0 39.5 20.5 41.0 66-67 32.73936793055266 36.0 32.0 39.0 19.5 41.0 68-69 32.33909447848106 35.0 32.0 39.0 18.0 40.5 70-71 31.90926133166417 35.0 31.0 38.0 15.5 40.0 72-73 31.51876292179587 35.0 31.0 37.5 12.5 40.0 74-75 31.095667760474953 35.0 31.0 37.0 9.0 39.0 76-77 30.675895436985893 35.0 30.5 36.5 7.0 39.0 78-79 30.235157717038753 34.0 30.0 36.0 6.0 38.5 80-81 29.7796077464388 34.0 29.5 35.5 2.0 37.0 82-83 29.29996901930287 34.0 29.0 35.0 2.0 37.0 84-85 28.993709337835018 34.0 29.0 35.0 2.0 36.5 86-87 28.68328812680589 34.0 29.0 35.0 2.0 36.0 88-89 28.288236502848328 34.0 28.5 35.0 2.0 36.0 90-91 27.887015294348238 34.0 27.0 35.0 2.0 35.0 92-93 27.537719947143138 34.0 27.0 35.0 2.0 35.0 94-95 27.08868382617932 33.0 26.0 35.0 2.0 35.0 96-97 26.514665250406228 33.0 25.0 35.0 2.0 35.0 98-99 25.83565404045194 33.0 23.5 35.0 2.0 35.0 100-101 23.40032751022679 30.5 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.41031601685498487 1101 2 0.45593748122824707 1101 3 0.48136101635865103 1101 4 0.4946404531924955 1101 5 0.39060129200163374 1101 6 0.2893068675553607 1101 7 0.3549101853011578 1101 8 0.60648629773344 1101 9 0.6812488512869521 1101 10-11 0.6452025416074179 1101 12-13 0.6531474569426408 1101 14-15 0.8051531474573395 1101 16-17 0.7808822871839354 1101 18-19 0.8940964129718125 1101 20-21 0.9204529843199936 1101 22-23 0.7432481260552777 1101 24-25 0.8126774082071222 1101 26-27 0.7276666456108885 1101 28-29 0.7591209752915518 1101 30-31 0.7464759265032654 1101 32-33 0.6596047998693209 1101 34-35 0.5814393157081241 1101 36-37 0.4846215907540312 1101 38-39 0.485427542035616 1101 40-41 0.501793215004966 1101 42-43 0.39900743803784167 1101 44-45 0.48454234544522023 1101 46-47 0.5844410252594585 1101 48-49 0.5292453551576131 1101 50-51 0.4006728656436067 1101 52-53 0.34009333641513706 1101 54-55 0.47466731864260225 1101 56-57 0.42873601708839004 1101 58-59 0.3792995447515537 1101 60-61 0.29231538922552147 1101 62-63 0.3456392431407167 1101 64-65 0.3975677790327694 1101 66-67 0.4500900789993807 1101 68-69 0.21890426826798404 1101 70-71 0.1864949089457788 1101 72-73 0.3991443272460131 1101 74-75 0.27914646127443277 1101 76-77 0.17144072641365682 1101 78-79 0.005348442757775018 1101 80-81 0.22941620678667363 1101 82-83 0.3840357117591857 1101 84-85 0.2953637380460101 1101 86-87 0.21670954046178537 1101 88-89 0.36989987716274086 1101 90-91 0.033709022528103816 1101 92-93 0.0989525628894583 1101 94-95 0.25387669800698376 1101 96-97 -0.02017371466262574 1101 98-99 -0.05893630245525827 1101 100-101 0.3637360535424605 1102 1 0.31485259770930796 1102 2 0.27360250063788527 1102 3 0.2398366412039863 1102 4 0.2502471262082224 1102 5 0.3578305006933533 1102 6 0.2878489575970775 1102 7 0.4119361489715203 1102 8 0.16093326680550035 1102 9 0.44950990778112043 1102 10-11 0.3893002871899469 1102 12-13 0.37770745934229666 1102 14-15 0.38744319717500986 1102 16-17 0.28366000247093126 1102 18-19 0.4537038708717702 1102 20-21 0.43613817075499384 1102 22-23 0.3848138076172489 1102 24-25 0.19162651321413193 1102 26-27 0.26063272061482934 1102 28-29 0.3692016133974292 1102 30-31 0.2116110690697397 1102 32-33 0.23051246598856068 1102 34-35 0.10034683934838995 1102 36-37 0.3062547748593758 1102 38-39 0.1199636354618363 1102 40-41 3.856101266919154E-4 1102 42-43 0.12369742126531946 1102 44-45 -0.034366115556785815 1102 46-47 0.12034385459212871 1102 48-49 0.4575893619166109 1102 50-51 0.46443721040275676 1102 52-53 0.35399097401359114 1102 54-55 0.44844368662832323 1102 56-57 0.4126661998864307 1102 58-59 0.5497431543191382 1102 60-61 0.47537620025589433 1102 62-63 0.6004032279529525 1102 64-65 0.6680132008330446 1102 66-67 0.5084902149793251 1102 68-69 0.4291576308970022 1102 70-71 0.13484346503376443 1102 72-73 0.21097270755991815 1102 74-75 0.2873465017684609 1102 76-77 0.3931352139767412 1102 78-79 0.42783487600887327 1102 80-81 0.5013585952843691 1102 82-83 0.5304638011160101 1102 84-85 0.4777265270346369 1102 86-87 0.3290523448981304 1102 88-89 0.24953388114715125 1102 90-91 0.2698901233157365 1102 92-93 0.09271754505459029 1102 94-95 0.3678005192433531 1102 96-97 0.15353785038152878 1102 98-99 0.5591359693846449 1102 100-101 0.5536499162477106 1103 1 0.16336456642705244 1103 2 0.3078134322862027 1103 3 0.1664635756943369 1103 4 0.3162787860782714 1103 5 0.32692430398927286 1103 6 0.1539071994358565 1103 7 0.2032332248307398 1103 8 0.18427509229115202 1103 9 0.49926197502605163 1103 10-11 0.33368931802875323 1103 12-13 0.3310977161812474 1103 14-15 0.25757615049383986 1103 16-17 0.4264036218668181 1103 18-19 0.31060127068321464 1103 20-21 0.5714121327229051 1103 22-23 0.4230454173045075 1103 24-25 0.3633115616020106 1103 26-27 0.17889673794527283 1103 28-29 0.06522220572816195 1103 30-31 0.06763399663883973 1103 32-33 0.26666208023947746 1103 34-35 0.05359440442762775 1103 36-37 0.12007634277320278 1103 38-39 0.23205709592487977 1103 40-41 0.12507786794197528 1103 42-43 -0.015362918561550032 1103 44-45 0.04248372240500231 1103 46-47 0.18934486942711004 1103 48-49 0.296848103844205 1103 50-51 0.4312771360229348 1103 52-53 0.18200082300565157 1103 54-55 0.18219763200113448 1103 56-57 0.2187338137324062 1103 58-59 0.20277113867304308 1103 60-61 0.07002431212871585 1103 62-63 -0.02976441856151979 1103 64-65 0.1595158211444101 1103 66-67 0.15384886660275043 1103 68-69 0.22208192072989874 1103 70-71 0.32084970434352655 1103 72-73 0.3418174800418683 1103 74-75 0.08045711381208065 1103 76-77 0.12380692417862704 1103 78-79 0.0920005810459088 1103 80-81 -0.01357264560913407 1103 82-83 0.015054488483489337 1103 84-85 -0.06254562139709208 1103 86-87 0.18290523978631512 1103 88-89 0.28327528082444076 1103 90-91 0.2725941243988892 1103 92-93 0.04823234090134321 1103 94-95 0.25077650727898515 1103 96-97 0.3444524377958409 1103 98-99 0.2521001011169304 1103 100-101 0.6265413388426317 1104 1 0.0803765280352593 1104 2 0.14314636402481895 1104 3 0.19332492687651026 1104 4 0.294780027154836 1104 5 0.34778779053036857 1104 6 0.19595335966718608 1104 7 0.15098114080852554 1104 8 0.17229704392979528 1104 9 0.16522905625344464 1104 10-11 0.2873204828540139 1104 12-13 0.10428190112492075 1104 14-15 0.1940251826030135 1104 16-17 0.19478743681396082 1104 18-19 0.4494706347346096 1104 20-21 0.5602404274074004 1104 22-23 0.222670954013509 1104 24-25 0.25676136981055464 1104 26-27 -0.05275155193745462 1104 28-29 0.3439087242431853 1104 30-31 0.30853390587677865 1104 32-33 0.09014677879839184 1104 34-35 0.011294972760666155 1104 36-37 -0.08980828456571999 1104 38-39 0.01633605866900467 1104 40-41 0.2511000267886061 1104 42-43 0.17573123265188428 1104 44-45 0.2662301916491927 1104 46-47 0.2737666133614667 1104 48-49 0.21118931583720268 1104 50-51 0.24380540223183544 1104 52-53 0.3037975519732612 1104 54-55 0.3062038556852542 1104 56-57 0.29585892082605625 1104 58-59 0.38803415322661294 1104 60-61 0.4345144781246475 1104 62-63 0.566714162398803 1104 64-65 0.2430913624132245 1104 66-67 0.35670005466756294 1104 68-69 0.2250555724356076 1104 70-71 0.3322816604055028 1104 72-73 0.41847931599146193 1104 74-75 0.14791478972018268 1104 76-77 0.44176540441352685 1104 78-79 0.2614095481885528 1104 80-81 0.33646119629365856 1104 82-83 0.20533547243603678 1104 84-85 0.25616848555820937 1104 86-87 0.3863537017633405 1104 88-89 0.6535945672348866 1104 90-91 0.5856675523323851 1104 92-93 0.31009313684056394 1104 94-95 0.35095737999682797 1104 96-97 0.3208311928977139 1104 98-99 0.8869132448032602 1104 100-101 1.2915274391573526 1105 1 0.30090078372143125 1105 2 0.3379930273387366 1105 3 0.4043312878489438 1105 4 0.4754005270073165 1105 5 0.4626319747887493 1105 6 0.37945221084444114 1105 7 0.3247012799713431 1105 8 0.3615887506497799 1105 9 0.47910912032153163 1105 10-11 0.3906807974358344 1105 12-13 0.4424776909913959 1105 14-15 0.27452621651119813 1105 16-17 0.398686042151553 1105 18-19 0.46723526261481396 1105 20-21 0.4542990544883665 1105 22-23 0.3155735600560803 1105 24-25 0.3949896972808702 1105 26-27 0.4203655440555778 1105 28-29 0.23782984121264406 1105 30-31 0.3291357288864205 1105 32-33 0.33731975167016515 1105 34-35 0.3691842441959423 1105 36-37 0.3244964468796141 1105 38-39 0.23822653864385757 1105 40-41 -0.029727577494732316 1105 42-43 0.30604046255007233 1105 44-45 0.11726832843237389 1105 46-47 0.15419446994035724 1105 48-49 0.0967337751803754 1105 50-51 0.19334241924371298 1105 52-53 0.06571106004988536 1105 54-55 0.3426824476850072 1105 56-57 0.3833343965755276 1105 58-59 0.32178413789390703 1105 60-61 0.14434441749754257 1105 62-63 0.12342255768161436 1105 64-65 0.468707042955927 1105 66-67 0.4065069594828117 1105 68-69 0.5085864124674444 1105 70-71 0.45534465617626196 1105 72-73 0.5456876283163012 1105 74-75 0.6402106727449137 1105 76-77 0.2110670144438913 1105 78-79 0.3056613350130135 1105 80-81 0.17995499897762457 1105 82-83 0.13562736831311994 1105 84-85 0.4547663343319499 1105 86-87 0.4773308434510497 1105 88-89 0.29187825382287613 1105 90-91 0.4210652051057977 1105 92-93 0.45011280788797237 1105 94-95 0.46043818571569517 1105 96-97 0.5975539912349426 1105 98-99 0.5533093565596019 1105 100-101 0.8029603074310607 1106 1 0.012235350841830694 1106 2 0.12472095591889598 1106 3 0.1513520660965355 1106 4 0.23929515776281107 1106 5 0.05613141744737504 1106 6 0.12134738524988364 1106 7 0.19313064588448725 1106 8 0.3487755758876929 1106 9 0.40864233606440337 1106 10-11 0.30755745501718934 1106 12-13 0.3912031190489458 1106 14-15 0.4744409623147732 1106 16-17 0.4755433847986197 1106 18-19 0.40895668943546326 1106 20-21 0.3501543581141888 1106 22-23 0.4103907193582046 1106 24-25 0.12617099385260389 1106 26-27 -0.1251654730440137 1106 28-29 0.290560546815513 1106 30-31 0.23908485687591252 1106 32-33 0.2333592217250029 1106 34-35 0.1612458195652593 1106 36-37 -0.06542529157133714 1106 38-39 0.06665472241891024 1106 40-41 -0.04291192358203233 1106 42-43 -0.18565067615613629 1106 44-45 0.044924649498405245 1106 46-47 0.014895357612523696 1106 48-49 0.20114011144055866 1106 50-51 -0.01733278864896448 1106 52-53 0.17199178645704905 1106 54-55 0.2345019518949556 1106 56-57 0.028641542099400397 1106 58-59 0.13940410654387847 1106 60-61 0.3787777626077329 1106 62-63 0.40426701168409096 1106 64-65 0.21189956382868047 1106 66-67 0.18808891404462713 1106 68-69 0.14624021564613088 1106 70-71 0.2301700570513141 1106 72-73 0.31436190385599616 1106 74-75 0.174716081527599 1106 76-77 0.11541634760446584 1106 78-79 0.29362857175520674 1106 80-81 0.5482977081576266 1106 82-83 0.5231803224227853 1106 84-85 0.5341013248848618 1106 86-87 0.7091977133297007 1106 88-89 0.75325523098223 1106 90-91 0.8407953570405979 1106 92-93 1.010210003682765 1106 94-95 1.055507634659019 1106 96-97 1.2415646251347887 1106 98-99 1.4006418986841211 1106 100-101 1.4101460039608504 1107 1 0.1604800356531335 1107 2 0.16720197380185908 1107 3 0.10855233935020081 1107 4 0.209275133964141 1107 5 0.30446244101223385 1107 6 0.34339994870103396 1107 7 0.273656228148603 1107 8 0.3968326926958028 1107 9 0.31008227315859216 1107 10-11 0.4370384820434339 1107 12-13 0.3147254005864184 1107 14-15 0.3332519562031351 1107 16-17 0.3117410705020447 1107 18-19 0.43718462896959664 1107 20-21 0.5049182066020919 1107 22-23 0.40839976860003446 1107 24-25 0.4110294492379367 1107 26-27 0.22964729202967504 1107 28-29 0.26256282218780314 1107 30-31 0.33530946302881404 1107 32-33 0.28892199757471104 1107 34-35 0.37983568958690483 1107 36-37 0.3488560656581967 1107 38-39 0.2904974108587979 1107 40-41 0.2073453946396313 1107 42-43 0.4077692266899078 1107 44-45 0.4659024848643796 1107 46-47 0.39785801773433604 1107 48-49 0.33327824299678355 1107 50-51 0.45355857734506344 1107 52-53 0.5694916199186082 1107 54-55 0.6951534894692912 1107 56-57 0.54338770485775 1107 58-59 0.5899232493215294 1107 60-61 0.4767833504623056 1107 62-63 0.4376563367658264 1107 64-65 0.49528954200235376 1107 66-67 0.4719510776859508 1107 68-69 0.4192731353981145 1107 70-71 0.6229842824385621 1107 72-73 0.5942604525551047 1107 74-75 0.5511772114550126 1107 76-77 0.5920963939478696 1107 78-79 0.6196376967556922 1107 80-81 0.6184152908292688 1107 82-83 0.907803468784607 1107 84-85 0.7408610876296251 1107 86-87 0.6539823823884277 1107 88-89 0.6507717182880803 1107 90-91 0.6293947230189509 1107 92-93 0.5076991917458145 1107 94-95 0.6063518212917955 1107 96-97 0.9069497077313251 1107 98-99 1.3702368771908695 1107 100-101 1.88596298931461 1108 1 -0.04303936470552472 1108 2 -0.002914018648894512 1108 3 0.09503623003386252 1108 4 0.12792862750630718 1108 5 0.29097252570620213 1108 6 -0.055879380194987505 1108 7 0.038235881447370446 1108 8 0.18170262936002501 1108 9 0.2005510116147562 1108 10-11 0.05916033120622899 1108 12-13 0.07818979352344257 1108 14-15 0.20879010878660864 1108 16-17 0.23959034076378316 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-2.2917398946457936 2316 60-61 -1.6682452134281718 2316 62-63 -1.6589634962119533 2316 64-65 -2.003892131822923 2316 66-67 -2.03520909175802 2316 68-69 -2.531056065531608 2316 70-71 -2.4774990345364536 2316 72-73 -1.9329106836901744 2316 74-75 -1.4611555177526085 2316 76-77 -1.5260078851719925 2316 78-79 -2.0384914693244056 2316 80-81 -1.7760952049114955 2316 82-83 -1.5270329102808127 2316 84-85 -1.5617992476945979 2316 86-87 -2.20429582980638 2316 88-89 -2.4363794040370372 2316 90-91 -2.309774477792633 2316 92-93 -2.9671111288608465 2316 94-95 -3.1386656182849677 2316 96-97 -4.2819241573179525 2316 98-99 -5.78036836423135 2316 100-101 -6.243416224757549 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3948.0 3 1390.0 4 1414.0 5 1524.0 6 1653.0 7 1967.0 8 2447.0 9 2948.0 10 3920.0 11 5164.0 12 6928.0 13 8506.0 14 9419.0 15 10898.0 16 13735.0 17 17909.0 18 14916.0 19 10082.0 20 10026.0 21 10359.0 22 11424.0 23 12656.0 24 14629.0 25 16839.0 26 19461.0 27 23327.0 28 28002.0 29 34357.0 30 42094.0 31 52560.0 32 66749.0 33 87069.0 34 116918.0 35 160145.0 36 221532.0 37 283992.0 38 217687.0 39 33028.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.77178270766043 17.869261063617582 13.794254944657405 22.564701284064583 2 15.784334681167797 19.957920751380826 40.45463801348509 23.803106553966288 3 19.46735384323521 25.445094381382994 29.3774939134284 25.710057861953395 4 12.595617579022674 16.262373434996494 37.00059522792928 34.14141375805155 5 13.458154154853222 37.33592438383478 33.71414690800885 15.491774553303147 6 31.211118146435822 35.547035854643305 18.268960112239082 14.972885886681794 7 28.697648263634335 32.738467610886595 20.748527595060335 17.81535653041874 8 27.03456764666629 33.496986376232066 19.821225601475533 19.647220375626112 9 27.197907152670076 16.59875623338081 20.475849523467364 35.727487090481745 10-11 25.20873758003887 26.245886369710398 27.098232147121287 21.447143903129444 12-13 26.56192627690895 23.835064405630387 27.639406903352498 21.96360241410817 14-15 23.150071408964006 24.989848415657097 26.639085560462327 25.22099461491657 16-17 22.143143145042686 28.330224358548605 27.113897275424804 22.412735220983905 18-19 21.976148944952172 26.429568856044444 29.971360389410144 21.622921809593244 20-21 22.854461774175036 25.47616887155178 30.026959427437028 21.642409926836155 22-23 22.34751245849671 25.575070169453262 29.969731568048957 22.107685804001072 24-25 22.321154766624364 25.726771664994256 30.104852900542443 21.847220667838936 26-27 22.001069894730453 25.937074761107432 30.091231921814217 21.970623422347902 28-29 21.738619101288773 26.256107565873187 30.13052131068245 21.874752022155587 30-31 21.91917374827182 25.83386631771602 30.085263801186656 22.161696132825504 32-33 21.685311026490954 26.105676136642053 29.95793756550027 22.251075271366723 34-35 22.492591771768712 26.045284958727954 29.619919084095443 21.842204185407883 36-37 22.497639110680577 25.69444290757865 29.644043967078122 22.16387401466265 38-39 22.165742111350056 26.02446693953326 29.71252895229219 22.097261996824496 40-41 22.298421285240355 25.983922640510926 29.710015724552875 22.007640349695848 42-43 22.16093778155734 26.17252727450912 29.19154909095508 22.474985852978463 44-45 22.198125766427996 26.192640204753836 29.18750650051071 22.421727528307454 46-47 22.56127959656644 26.51889024157772 28.619895948688168 22.29993421316767 48-49 22.629744549488105 26.63953199809688 28.258096537247468 22.47262691516754 50-51 22.455554456124755 26.862947731338593 28.29862170367024 22.382876108866412 52-53 22.784520801331063 26.90182401483061 27.997333069464435 22.31632211437389 54-55 22.25188028162335 26.80590814678052 28.182062476881782 22.760149094714347 56-57 22.479621882814406 27.138900016409362 27.861417737095493 22.520060363680745 58-59 22.685367560906055 26.71965667884714 27.942994373038644 22.651981387208163 60-61 22.433751829359093 26.812895254589563 28.456117354670084 22.297235561381264 62-63 22.38883779630319 26.887900899844237 28.374637183803376 22.348624120049205 64-65 22.07674993519344 27.045453108501988 28.23533316894596 22.64246378735861 66-67 22.17454368871879 27.132619388305457 27.913250431066917 22.77958649190884 68-69 22.245036368609014 26.998961348571104 28.1548839540211 22.601118328798783 70-71 22.382808325845872 26.93579583859458 27.89783709856197 22.783558736997584 72-73 22.38882314986085 26.957483621708654 28.01237109099985 22.641322137430645 74-75 22.164842366629774 27.487922567860767 27.720982785727177 22.626252279782282 76-77 22.391388265629857 27.129720113574535 27.71317909108125 22.76571252971436 78-79 22.42537384211691 27.194018526129433 27.701748284910373 22.678859346843286 80-81 22.409210403595385 27.50496768043706 27.493206489521633 22.59261542644592 82-83 22.463239141355622 27.432922162234963 27.518534946487268 22.585303749922147 84-85 22.310189116879126 27.419481308686024 27.455525251944003 22.81480432249085 86-87 22.442123819050234 27.338694686152838 27.45165234963321 22.767529145163717 88-89 22.370730168224583 27.36590419567177 27.237612046183905 23.025753589919738 90-91 22.330110592429183 27.394309336337937 27.43101330131458 22.844566769918302 92-93 22.53314809521762 27.193484457957183 27.566100160112512 22.707267286712685 94-95 22.242115689770245 27.064372317187797 27.598682120128654 23.094829872913305 96-97 22.17238202490169 27.05762064977594 27.67678006751822 23.093217257804145 98-99 22.199702529260144 27.169436905090137 27.290252774243484 23.34060779140623 100-101 22.177734699266487 27.066632723891864 27.398428618186475 23.35720395865517 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 266.0 1 260.5 2 677.5 3 949.0 4 603.5 5 462.5 6 754.0 7 1484.0 8 2301.0 9 2658.0 10 2389.5 11 1958.5 12 1921.5 13 2099.0 14 2297.5 15 2466.0 16 2592.5 17 2675.5 18 2798.5 19 3021.5 20 3327.0 21 3786.0 22 4363.0 23 5131.0 24 6182.5 25 7438.5 26 9086.0 27 11277.0 28 13254.0 29 15374.5 30 18441.5 31 21888.5 32 26058.0 33 28882.0 34 31083.5 35 33706.5 36 36395.5 37 39327.5 38 40678.5 39 41425.5 40 42604.5 41 44879.5 42 47177.5 43 49377.0 44 51430.5 45 53815.5 46 55277.0 47 57216.0 48 63952.0 49 64005.0 50 57897.0 51 54562.5 52 52403.0 53 52267.0 54 51691.0 55 48917.0 56 45098.5 57 40910.5 58 37721.0 59 33973.5 60 28554.0 61 24035.0 62 20632.0 63 16452.5 64 12531.5 65 9894.0 66 7484.5 67 5986.0 68 4563.5 69 3071.5 70 2351.0 71 1918.0 72 1678.5 73 1494.0 74 1181.0 75 939.5 76 691.0 77 439.5 78 278.0 79 199.0 80 157.5 81 101.5 82 60.5 83 43.5 84 29.0 85 21.0 86 16.5 87 13.5 88 7.5 89 4.5 90 3.0 91 1.0 92 0.5 93 3.5 94 3.5 95 2.0 96 1.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03028521209132351 2 0.020738099302618187 3 0.017703255502235036 4 0.04571233474327118 5 0.05083363365641774 6 0.04545943109323925 7 0.045080075618191356 8 0.040654261742632605 9 0.03957942122999691 10-11 0.04789362872479657 12-13 0.04258265207412606 14-15 0.03768264385475743 16-17 0.04571233474327118 18-19 0.04915814697495622 20-21 0.011349051295182818 22-23 0.017671642545981043 24-25 0.005121298913146564 26-27 0.010495501476325057 28-29 0.0055322673444484485 30-31 0.007998077932259758 32-33 0.003319360406669069 34-35 0.007049689244640024 36-37 0.008124529757275721 38-39 0.009420660963689358 40-41 0.0017070996377155216 42-43 0.0015806478126995567 44-45 0.0014858089439375834 46-47 4.109684313018847E-4 48-49 7.903239063497783E-4 50-51 2.2129069377793798E-4 52-53 0.0023077458065413527 54-55 0.005374202563178493 56-57 0.013625184145470178 58-59 0.00866195001359357 60-61 0.008440659319815634 62-63 0.004963234131876609 64-65 0.0 66-67 0.005627106213210422 68-69 0.015964542908265524 70-71 0.00613291351327428 72-73 0.0024974235440652997 74-75 0.004078071356764856 76-77 0.007176141069655988 78-79 0.005058073000638582 80-81 0.009958081220007207 82-83 0.005721945081972395 84-85 0.014352282139311976 86-87 0.004046458400510865 88-89 0.0052793636944165195 90-91 0.0036038770129549896 92-93 0.01530067082693171 94-95 0.009515499832451332 96-97 0.008282594538545678 98-99 0.018841321927378717 100-101 0.03398392797304047 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1581630.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.588532715959566 #Duplication Level Percentage of deduplicated Percentage of total 1 82.07975546938582 50.55171705044229 2 10.887789658827916 13.411259792144186 3 3.0388059788441666 5.614668043364924 4 1.261903577641052 3.108751591037294 5 0.6535348064474581 2.0125124903953786 6 0.427501886498343 1.5797528353642576 7 0.2876296974909409 1.2400283723801655 8 0.20574284958802422 1.0137120178341423 9 0.15622650274592478 0.8659584967920603 >10 0.8876825487318026 10.204224868954025 >50 0.0712908202425758 3.004477689503785 >100 0.037820067109672915 4.202916039877829 >500 0.003288484911523729 1.3693759795816929 >1k 8.221212278809322E-4 0.9343731552303813 >5k 2.0553030697023305E-4 0.8862715770975431 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7863 0.4971453500502646 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6140 0.38820710279901116 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3872 0.24481073323090735 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2638 0.16678995719605721 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1770 0.11190986513912862 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1686 0.10659888848845811 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1616 0.10217307461289935 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.1612956253991134E-5 0.0 20-21 0.0 0.0 0.0 6.322591250798227E-5 1.2645182501596453E-4 22-23 0.0 0.0 0.0 9.48388687619734E-5 1.2645182501596453E-4 24-25 0.0 0.0 0.0 1.2645182501596453E-4 1.2645182501596453E-4 26-27 0.0 0.0 0.0 3.161295625399113E-4 1.2645182501596453E-4 28-29 0.0 0.0 0.0 7.270979938417961E-4 1.2645182501596453E-4 30-31 0.0 0.0 0.0 0.001643873725207539 1.5806478126995565E-4 32-33 0.0 0.0 0.0 0.002876779019113193 1.896777375239468E-4 34-35 0.0 0.0 0.0 0.004931621175622618 1.896777375239468E-4 36-37 0.0 0.0 0.0 0.008187755669783704 1.896777375239468E-4 38-39 0.0 0.0 0.0 0.011349051295182818 1.896777375239468E-4 40-41 0.0 0.0 0.0 0.015806478126995566 1.896777375239468E-4 42-43 0.0 0.0 0.0 0.022634876677857654 1.896777375239468E-4 44-45 0.0 0.0 0.0 0.031391665560213194 2.2129069377793795E-4 46-47 0.0 0.0 0.0 0.04906330810619425 2.845166062859202E-4 48-49 0.0 0.0 0.0 0.074859480409451 3.1612956253991136E-4 50-51 0.0 0.0 0.0 0.099865328806358 3.1612956253991136E-4 52-53 0.0 0.0 0.0 0.12635698614720256 3.477425187939025E-4 54-55 0.0 0.0 0.0 0.15683187597605003 3.793554750478936E-4 56-57 0.0 0.0 0.0 0.19034160960528063 3.793554750478936E-4 58-59 0.0 0.0 0.0 0.2308061936103893 3.793554750478936E-4 60-61 0.0 0.0 0.0 0.28590757636109587 3.793554750478936E-4 62-63 0.0 0.0 0.0 0.33737346914259336 3.793554750478936E-4 64-65 0.0 0.0 0.0 0.3950671143061272 3.793554750478936E-4 66-67 0.0 0.0 0.0 0.47542724910377265 3.793554750478936E-4 68-69 0.0 0.0 0.0 0.5734590264473992 3.793554750478936E-4 70-71 0.0 0.0 0.0 0.6712062871847397 3.793554750478936E-4 72-73 0.0 0.0 0.0 0.7721464565037335 3.793554750478936E-4 74-75 0.0 0.0 0.0 0.8715692039225356 3.793554750478936E-4 76-77 0.0 0.0 0.0 0.9739003433167049 3.793554750478936E-4 78-79 0.0 0.0 0.0 1.0929231236129815 3.793554750478936E-4 80-81 0.0 0.0 0.0 1.2505137105391273 3.793554750478936E-4 82-83 0.0 0.0 0.0 1.419832704235504 3.793554750478936E-4 84-85 0.0 0.0 0.0 1.601512363827191 3.793554750478936E-4 86-87 0.0 0.0 0.0 1.8287462933808794 3.793554750478936E-4 88-89 0.0 0.0 0.0 2.092050606020371 3.793554750478936E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1680 0.0 40.732403 2 GTACCTG 1910 0.0 37.56788 1 TATAACG 95 8.672432E-7 35.01557 2 GTATCAA 11180 0.0 27.627693 1 GGTATCA 7885 0.0 27.481234 1 GTACATG 8035 0.0 26.84992 1 TACATGG 8200 0.0 25.673004 2 ACATGGG 8060 0.0 24.880795 3 ATCAACG 13170 0.0 23.381699 3 TCAACGC 13215 0.0 23.303553 4 TATCAAC 13150 0.0 23.236572 2 CAACGCA 13305 0.0 23.217358 5 AACGCAG 13615 0.0 22.75781 6 GAGTACT 7110 0.0 22.52729 12-13 ATAACGC 175 4.0352406E-6 21.723948 3 TAACGCA 200 5.3624535E-7 21.385864 4 ACGCAGA 14855 0.0 20.860115 7 CATGGGA 4480 0.0 20.791813 4 ACCTGGG 3155 0.0 20.78579 3 GTAGTAC 390 0.0 20.714342 3 >>END_MODULE