FastQCFastQC Report
Thu 26 May 2016
SRR936393_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936393_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1581630
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74650.4719814368720877No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66270.41899812219039856No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45140.285401769061032No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31050.19631645833728495No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18620.11772664908986298No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16630.10514469250077453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG17600.039.6760032
GTACCTG18650.037.4517061
GGTATCA91050.026.3538781
GTACATG81300.025.4817391
GTATCAA122350.024.3887081
ACATGGG81100.024.3651033
TACATGG83550.024.3343942
ACCTGGG29250.022.2479763
GAGTACT83750.020.75831212-13
CATGGGA47350.020.364414
CATGGGG34250.020.2482764
AGTACTT86150.020.06974412-13
ATCAACG149350.019.8142953
TATCAAC150450.019.765392
TCAACGC150750.019.661794
CAACGCA152450.019.5048545
AACGCAG154950.019.3146
TATACCG1250.006031949519.0000955
GTACTTT92650.018.73803514-15
AGAGTAC135250.018.33338710-11
Produced by FastQC (version 0.11.5)