FastQCFastQC Report
Thu 26 May 2016
SRR936392_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936392_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1596601
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77870.4877236078394039No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62810.3933982253549885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38760.2427657254379773No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26480.1658523325489587No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17810.1115494729115164No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16730.10478510285287307No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16060.10058868809427025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG19600.035.407072
GTACCTG20400.035.1846771
TATAACG1106.973096E-834.5692942
GTATCAA112800.028.697551
GTACATG84500.027.5642491
GTACACG2600.027.4236161
GGTATCA79300.026.7942221
TAACGCA1806.6465873E-926.4095824
TACATGG86950.026.404062
ACATGGG86150.025.5473653
ATCAACG134500.023.8562343
TCAACGC134650.023.7951014
ATAACGC2002.0389052E-823.767883
TATCAAC135750.023.635082
CAACGCA136250.023.5171475
ACCTGGG28550.023.3100053
AACGCAG138800.023.0850966
CATGGGG36150.022.7494984
GTATAGG4100.022.0280741
GAGTACT71550.021.88828512-13
Produced by FastQC (version 0.11.5)