##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936392_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1596601 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.51759957559841 31.0 31.0 34.0 30.0 34.0 2 31.578049869691927 33.0 31.0 34.0 30.0 34.0 3 31.535086724861127 33.0 31.0 34.0 28.0 34.0 4 35.21486457793775 37.0 35.0 37.0 33.0 37.0 5 35.2591016791296 37.0 35.0 37.0 33.0 37.0 6 35.32732285649326 37.0 35.0 37.0 33.0 37.0 7 35.20750394118505 37.0 35.0 37.0 32.0 37.0 8 35.18701291055185 37.0 35.0 37.0 32.0 37.0 9 36.71114072958742 39.0 37.0 39.0 32.0 39.0 10-11 36.59386847434018 39.0 37.0 39.0 32.0 39.0 12-13 36.49545503228421 39.0 37.0 39.0 32.0 39.0 14-15 37.512963476786 40.0 37.0 41.0 32.0 41.0 16-17 37.433339638394315 40.0 37.0 41.0 32.0 41.0 18-19 37.32722577525631 40.0 37.0 41.0 32.0 41.0 20-21 37.30880132230908 39.5 37.0 41.0 32.0 41.0 22-23 37.28650771232137 39.0 37.0 41.0 31.5 41.0 24-25 37.38907435232723 40.0 37.0 41.0 32.0 41.0 26-27 37.30455041679167 40.0 37.0 41.0 32.0 41.0 28-29 37.182738830803686 39.5 36.5 41.0 31.0 41.0 30-31 36.96445605382935 39.0 36.0 41.0 31.0 41.0 32-33 36.76508157016061 39.0 35.5 41.0 30.5 41.0 34-35 36.54479390906056 39.0 35.0 41.0 30.0 41.0 36-37 36.32589388331837 39.0 35.0 40.5 30.0 41.0 38-39 36.121170223493536 39.0 35.0 40.0 30.0 41.0 40-41 35.87286585690476 38.5 35.0 40.0 28.5 41.0 42-43 35.588013536256085 38.0 35.0 40.0 27.5 41.0 44-45 35.40341231152931 38.0 35.0 40.0 27.0 41.0 46-47 35.19369836295981 38.0 35.0 40.0 26.0 41.0 48-49 34.916947314952196 38.0 34.0 40.0 24.5 41.0 50-51 33.922420191394096 37.5 33.0 39.5 23.5 40.5 52-53 34.019885368980724 37.5 33.5 39.5 23.5 40.5 54-55 34.44657368998266 38.0 34.0 40.0 24.0 41.0 56-57 34.29854891735631 38.0 34.0 40.0 23.0 41.0 58-59 34.0777307542711 38.0 34.0 40.0 23.0 41.0 60-61 33.78680866415591 37.0 33.0 40.0 22.0 41.0 62-63 33.45058690305217 37.0 33.0 40.0 21.5 41.0 64-65 33.094462548877274 36.0 33.0 39.5 20.0 41.0 66-67 32.74528232163202 36.0 32.0 39.0 19.5 41.0 68-69 32.31611247894747 35.0 32.0 39.0 18.0 40.0 70-71 31.924925200472753 35.0 31.0 38.0 16.0 40.0 72-73 31.518352111767435 35.0 31.0 37.5 13.0 39.5 74-75 31.110236057725132 35.0 31.0 37.0 9.0 39.0 76-77 30.66131550713046 35.0 30.5 36.0 7.0 39.0 78-79 30.21408636221573 34.0 30.0 36.0 6.0 38.5 80-81 29.77722142225891 34.0 29.5 35.5 2.0 37.0 82-83 29.385729120801003 34.0 29.0 35.0 2.0 37.0 84-85 29.019645797541152 34.0 29.0 35.0 2.0 36.0 86-87 28.68683973015174 34.0 29.0 35.0 2.0 36.0 88-89 28.34648168202325 34.0 28.5 35.0 2.0 36.0 90-91 27.998280096279537 34.0 27.5 35.0 2.0 35.0 92-93 27.593378683841486 34.0 27.0 35.0 2.0 35.0 94-95 27.11701044907275 33.0 26.0 35.0 2.0 35.0 96-97 26.554475413706992 33.0 25.0 35.0 2.0 35.0 98-99 25.849033665893984 33.0 23.5 35.0 2.0 35.0 100-101 23.31839921182562 29.5 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5123979977792317 1101 2 0.4247321594586637 1101 3 0.4782921241303377 1101 4 0.5754370488230691 1101 5 0.3900063239262863 1101 6 0.44940173804258876 1101 7 0.4554057266397038 1101 8 0.44446506889200066 1101 9 0.678339697134227 1101 10-11 0.717073272015341 1101 12-13 0.6712840715623685 1101 14-15 0.9213401955480762 1101 16-17 1.0448302142659855 1101 18-19 1.0429426665783552 1101 20-21 0.9314917738077355 1101 22-23 0.8305877195019988 1101 24-25 0.7777427576983982 1101 26-27 0.5953779350280541 1101 28-29 0.7340357019121697 1101 30-31 0.7229571040165368 1101 32-33 0.7219342044536248 1101 34-35 0.7570771810617316 1101 36-37 0.713140513198411 1101 38-39 0.7481291271496886 1101 40-41 0.7302295246200075 1101 42-43 0.7274177870798084 1101 44-45 0.7535181286988433 1101 46-47 0.8257201528290139 1101 48-49 0.9036821511233839 1101 50-51 0.8073872338110064 1101 52-53 0.7970941070772639 1101 54-55 0.7929457824581263 1101 56-57 0.9037758440826806 1101 58-59 0.8483393272236341 1101 60-61 0.7480868294961454 1101 62-63 0.6525385944315545 1101 64-65 0.5550862030386412 1101 66-67 0.5576895413437981 1101 68-69 0.8204442694877372 1101 70-71 0.6634395806509552 1101 72-73 0.7726263082457301 1101 74-75 0.6197682266616305 1101 76-77 0.7329622632393118 1101 78-79 0.7394247016405942 1101 80-81 0.7204235990219487 1101 82-83 0.767677138385455 1101 84-85 0.7230778003750835 1101 86-87 0.6564914248839315 1101 88-89 0.6545029056447191 1101 90-91 0.66898055525402 1101 92-93 0.5276802474515421 1101 94-95 0.634812439991137 1101 96-97 0.5810348347621002 1101 98-99 0.6768591058277806 1101 100-101 0.8365326901510137 1102 1 0.24252849542641997 1102 2 0.4316415115256973 1102 3 0.5684030049402828 1102 4 0.39793671098458816 1102 5 0.39791650266663225 1102 6 0.41201142452213446 1102 7 0.345258010332671 1102 8 0.3231655105246105 1102 9 0.4446944104092836 1102 10-11 0.4316173613645873 1102 12-13 0.32042041048446634 1102 14-15 0.5264810821250805 1102 16-17 0.3698018072838636 1102 18-19 0.48521923479921014 1102 20-21 0.44997312480636253 1102 22-23 0.2664041180866903 1102 24-25 0.4518302924418407 1102 26-27 0.3911079421274195 1102 28-29 0.19562536995493218 1102 30-31 0.1849277523555486 1102 32-33 0.3804445302568098 1102 34-35 -0.035830685150422426 1102 36-37 0.08442579670040828 1102 38-39 -0.0384408574398023 1102 40-41 -2.3214990418551906E-4 1102 42-43 -0.17488899944496694 1102 44-45 -0.19899177329944706 1102 46-47 -0.09459056975198621 1102 48-49 0.22187635527426153 1102 50-51 -0.026591164828253966 1102 52-53 -0.17160396809988043 1102 54-55 0.15036128952989714 1102 56-57 0.08049947063442886 1102 58-59 -0.0753353466908564 1102 60-61 -0.11092936581445656 1102 62-63 -0.10378691564110198 1102 64-65 0.08378804043586996 1102 66-67 0.006045774733401288 1102 68-69 0.013111810410606495 1102 70-71 -0.21040994450357786 1102 72-73 0.20022736319000245 1102 74-75 -0.023881647053396904 1102 76-77 -0.09676444604892609 1102 78-79 -0.15906752363327925 1102 80-81 0.01976387818756109 1102 82-83 -0.014671609899181703 1102 84-85 0.027327840186096353 1102 86-87 -0.2229127676123106 1102 88-89 -0.13167388570813898 1102 90-91 0.18242303738029975 1102 92-93 0.10259014132902067 1102 94-95 0.17276470737903438 1102 96-97 -0.03750253715270446 1102 98-99 0.07884042542171343 1102 100-101 0.8818888543868333 1103 1 0.21328433966184335 1103 2 0.26657824365597094 1103 3 0.360520673111143 1103 4 0.3654886755493294 1103 5 0.2992527655948578 1103 6 0.3326169227648137 1103 7 0.3510245560514207 1103 8 0.20606195780722203 1103 9 0.3551652319067884 1103 10-11 0.32662793154136693 1103 12-13 0.2639771983762529 1103 14-15 0.5241142816716291 1103 16-17 0.689442421273263 1103 18-19 0.7759122269689627 1103 20-21 0.5977903723585882 1103 22-23 0.5089557543850773 1103 24-25 0.44035738317028006 1103 26-27 0.48793010492127564 1103 28-29 0.47231999032583616 1103 30-31 0.4460231553134477 1103 32-33 0.45607709953195297 1103 34-35 0.2611300060472246 1103 36-37 0.2799861036075697 1103 38-39 0.2178837410904393 1103 40-41 0.28589575142332535 1103 42-43 0.4115380619583462 1103 44-45 0.4650659052883981 1103 46-47 0.6178130840714431 1103 48-49 0.61657616663053 1103 50-51 0.5115391612934346 1103 52-53 0.5325054890350955 1103 54-55 0.5432074901750497 1103 56-57 0.4195712646757457 1103 58-59 0.5721711420408511 1103 60-61 0.540960573040465 1103 62-63 0.6109693137601795 1103 64-65 0.5959787588139704 1103 66-67 0.5269071018924123 1103 68-69 0.41729086001384275 1103 70-71 0.49108651240818446 1103 72-73 0.5311745648287136 1103 74-75 0.448434556611808 1103 76-77 0.30460136085166667 1103 78-79 0.38172613688678325 1103 80-81 0.36699418548864315 1103 82-83 0.4015384017871 1103 84-85 0.6175047976384 1103 86-87 0.3695434525975152 1103 88-89 0.2050442385341107 1103 90-91 0.3982100231510479 1103 92-93 0.40115824346800366 1103 94-95 0.37199503218765173 1103 96-97 0.397127594197606 1103 98-99 0.5871751082945238 1103 100-101 0.9641841365427091 1104 1 0.3162458734182607 1104 2 0.339943755598469 1104 3 0.388792492933824 1104 4 0.45753037181559364 1104 5 0.35995358393881105 1104 6 0.26416190378172644 1104 7 0.3843957339473292 1104 8 0.3460839777625395 1104 9 0.4701926710031543 1104 10-11 0.5031599388126722 1104 12-13 0.5600404338502756 1104 14-15 0.5644863459458591 1104 16-17 0.5560800427747026 1104 18-19 0.6606712415223015 1104 20-21 0.7323074034848531 1104 22-23 0.5060165410512028 1104 24-25 0.4571256006055364 1104 26-27 0.17230216061072667 1104 28-29 0.05789198334640844 1104 30-31 0.18907431415114928 1104 32-33 0.29785856971809466 1104 34-35 0.28392688376349895 1104 36-37 0.14455908602380418 1104 38-39 0.014443820585491096 1104 40-41 0.23321526183344332 1104 42-43 0.24346206091959033 1104 44-45 0.4247807618904105 1104 46-47 0.5882685709249316 1104 48-49 0.5914892994091403 1104 50-51 0.42872824746529403 1104 52-53 0.39623287082806513 1104 54-55 0.4790341291701168 1104 56-57 0.49104383309105515 1104 58-59 0.4146114939308987 1104 60-61 0.5072449309312645 1104 62-63 0.39993293791001605 1104 64-65 0.473869951046467 1104 66-67 0.5485439614948078 1104 68-69 0.42055629490796065 1104 70-71 0.39116399002874047 1104 72-73 0.46523381267339303 1104 74-75 0.48889354176229105 1104 76-77 0.5258289411676316 1104 78-79 0.3408293280633501 1104 80-81 0.568639887412278 1104 82-83 0.7163100145020138 1104 84-85 0.586923672560328 1104 86-87 0.49645544360067007 1104 88-89 0.4161804124273729 1104 90-91 0.6064906852949683 1104 92-93 0.5988431434875352 1104 94-95 0.7571693353719624 1104 96-97 0.7671486912893002 1104 98-99 0.39984988900710405 1104 100-101 0.9565801695168439 1105 1 0.3299220220460981 1105 2 0.3671085985422806 1105 3 0.42222441449159476 1105 4 0.46056509680192903 1105 5 0.44903692927065464 1105 6 0.3638031199285692 1105 7 0.40392643837782316 1105 8 0.38826273949732126 1105 9 0.34265036043760944 1105 10-11 0.5187334457221198 1105 12-13 0.5354126986899175 1105 14-15 0.6865775282861861 1105 16-17 0.7201004074658286 1105 18-19 0.7169934002560225 1105 20-21 0.6092175952956325 1105 22-23 0.4717956464933266 1105 24-25 0.2961823539400186 1105 26-27 0.3782652734252352 1105 28-29 0.5441310486400113 1105 30-31 0.3347671659447755 1105 32-33 0.5752379214406531 1105 34-35 0.4112814317041682 1105 36-37 0.48030918229453334 1105 38-39 0.2468051560371336 1105 40-41 0.288175447832522 1105 42-43 0.3089355665032869 1105 44-45 0.32266271315414485 1105 46-47 0.5096777340493404 1105 48-49 0.424642321015412 1105 50-51 0.5546742674063623 1105 52-53 0.5484610335569116 1105 54-55 0.24389674334998546 1105 56-57 0.29253310826874923 1105 58-59 0.3431747522074815 1105 60-61 0.16622067810472885 1105 62-63 0.5053672014134349 1105 64-65 0.42205209127524057 1105 66-67 0.26382013133458315 1105 68-69 0.4135696137218048 1105 70-71 0.5082387654920772 1105 72-73 0.3495290260830366 1105 74-75 0.06699554835586952 1105 76-77 0.26671682714854583 1105 78-79 0.4563272366220268 1105 80-81 0.5584484466131556 1105 82-83 0.37519450102364615 1105 84-85 0.4836485250865117 1105 86-87 0.5550056263223802 1105 88-89 0.47063958978042564 1105 90-91 0.4459969590023114 1105 92-93 0.5559124063142029 1105 94-95 0.5603201093763666 1105 96-97 0.7982901554739641 1105 98-99 0.9746304395123211 1105 100-101 1.4228146532790724 1106 1 0.25499600264458167 1106 2 0.02602996549420311 1106 3 0.09691774867812697 1106 4 0.22444261491831696 1106 5 0.32419023865165286 1106 6 0.16754766431034085 1106 7 0.32346591111274847 1106 8 0.42156545800592227 1106 9 0.48666833033595935 1106 10-11 0.5420603110463773 1106 12-13 0.39204498014052547 1106 14-15 0.34709378331520924 1106 16-17 0.41348290017756284 1106 18-19 0.5033590127815017 1106 20-21 0.3256262944227899 1106 22-23 0.1792335118872188 1106 24-25 -0.04933370347281851 1106 26-27 -0.07383962124534094 1106 28-29 0.056017505281452884 1106 30-31 -0.01840604809111568 1106 32-33 -0.04232143712858516 1106 34-35 0.13287833985781106 1106 36-37 -0.010618509629992445 1106 38-39 0.04934204595990366 1106 40-41 0.09921406830843438 1106 42-43 0.057472422548997315 1106 44-45 -0.09132625421660379 1106 46-47 0.048412388211609425 1106 48-49 0.13305854335933986 1106 50-51 -0.29135825833549944 1106 52-53 -0.06874277271899842 1106 54-55 0.20942871670756347 1106 56-57 0.2531508690076194 1106 58-59 0.13805401499246983 1106 60-61 0.20140027984852082 1106 62-63 0.09028036635267966 1106 64-65 -0.10812515475816298 1106 66-67 -0.08113240627680085 1106 68-69 0.02819578349982521 1106 70-71 -0.209943146792682 1106 72-73 -0.08607491720676563 1106 74-75 0.010824873217426756 1106 76-77 0.06676845829762357 1106 78-79 0.3167902921195527 1106 80-81 0.34382911498245505 1106 82-83 0.4587944593674109 1106 84-85 0.49503168781120266 1106 86-87 0.4986097314692408 1106 88-89 0.7046262484922536 1106 90-91 0.7222472891468108 1106 92-93 0.8320807100584346 1106 94-95 0.9157677915894666 1106 96-97 0.8245999273119651 1106 98-99 1.035108833789856 1106 100-101 1.4862845801736349 1107 1 0.09765507766575965 1107 2 0.14524915636448554 1107 3 0.1495388222814249 1107 4 0.24554176227731261 1107 5 0.26520783011820726 1107 6 0.21025067017992427 1107 7 0.28105043017793463 1107 8 0.22315134929311853 1107 9 0.2659833793046644 1107 10-11 0.35941873432380333 1107 12-13 0.16189021645085688 1107 14-15 0.1470975162033099 1107 16-17 0.3306220394167525 1107 18-19 0.29752386082210336 1107 20-21 0.09153055725759884 1107 22-23 0.20354556529034795 1107 24-25 0.09475994204102278 1107 26-27 0.06345554037637413 1107 28-29 0.20352396687990648 1107 30-31 0.14040134660596237 1107 32-33 0.2975518544581064 1107 34-35 0.13317959318270312 1107 36-37 0.1553264139198447 1107 38-39 0.09392507001882677 1107 40-41 0.14314877299997164 1107 42-43 0.12297936417834876 1107 44-45 0.1126847186233988 1107 46-47 0.2005004300742499 1107 48-49 0.31296940445949417 1107 50-51 0.24197488989594262 1107 52-53 0.16192333493995648 1107 54-55 0.220741218674263 1107 56-57 0.40255231119814283 1107 58-59 0.48082924144920725 1107 60-61 0.38555614427743023 1107 62-63 0.4223186034669695 1107 64-65 0.4202281356438675 1107 66-67 0.4536938971952864 1107 68-69 0.3088876820220392 1107 70-71 0.41391731970109547 1107 72-73 0.3497164766659928 1107 74-75 0.3427659318398142 1107 76-77 0.37499765639304883 1107 78-79 0.3707735613767795 1107 80-81 0.2713243964267953 1107 82-83 0.43686466728002316 1107 84-85 0.23924077520335985 1107 86-87 0.3455832230276705 1107 88-89 0.4629880720913917 1107 90-91 0.5518759624166769 1107 92-93 0.6228677571218242 1107 94-95 0.5557074422034809 1107 96-97 0.7388760030778698 1107 98-99 0.8956879312207064 1107 100-101 1.1651297171459483 1108 1 0.03890228391730233 1108 2 -0.09505168117058815 1108 3 0.11822246881655829 1108 4 0.16839366289494961 1108 5 0.16121802846349453 1108 6 0.0846703585210733 1108 7 0.27062498618754915 1108 8 0.2619222488894266 1108 9 -0.05795586496544303 1108 10-11 0.10488823918657175 1108 12-13 0.08328583108793453 1108 14-15 0.3741121485577992 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78-79 -3.505358881610082 2316 80-81 -4.133311060720935 2316 82-83 -3.3622618895625003 2316 84-85 -3.3662114911043908 2316 86-87 -4.031622185400604 2316 88-89 -5.918584789086598 2316 90-91 -4.90604521443305 2316 92-93 -4.103758041441239 2316 94-95 -3.58703369066604 2316 96-97 -5.51611323883224 2316 98-99 -7.373842267258944 2316 100-101 -7.924470480006715 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4045.0 3 1429.0 4 1372.0 5 1533.0 6 1731.0 7 1934.0 8 2469.0 9 3012.0 10 3936.0 11 5208.0 12 6932.0 13 8745.0 14 9276.0 15 11298.0 16 13986.0 17 18368.0 18 14631.0 19 10059.0 20 9924.0 21 10279.0 22 11402.0 23 12932.0 24 14540.0 25 17048.0 26 19562.0 27 23586.0 28 28443.0 29 34318.0 30 42261.0 31 53055.0 32 66871.0 33 87961.0 34 116638.0 35 161710.0 36 224609.0 37 288003.0 38 220803.0 39 32674.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.74607838467305 17.91163485668586 13.82286357516941 22.51942318347168 2 15.716011282062848 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26.26475990348225 29.183529349131515 22.315893642574284 46-47 22.543451076650957 26.507669918692567 28.545914444776056 22.40296455988042 48-49 22.635537386265344 26.685170698282402 28.27158398310398 22.407707932348274 50-51 22.48124819340346 26.90304491570886 28.16809310153362 22.447613789354055 52-53 22.77559275197067 26.915829548988334 27.994403104848775 22.314174594192217 54-55 22.23372932088771 26.837984991848295 28.1864763788528 22.74180930841119 56-57 22.561435441582308 27.088999181988203 27.867425718140442 22.48213965828904 58-59 22.71747746156479 26.74346974931266 27.902969558432382 22.636083230690165 60-61 22.39905419583157 26.87264527645981 28.461846823569942 22.266453704138677 62-63 22.457486987729947 26.867378191999098 28.350902246691344 22.32423257357961 64-65 22.116483109022216 27.056881156864122 28.222394951246155 22.604240782867507 66-67 22.189565919973443 27.175207237903166 27.87297342156276 22.76225342056063 68-69 22.22639786390874 27.00751803407449 28.156080475471967 22.610003626544806 70-71 22.368082746949177 26.87747580216068 27.97209783295967 22.78234361793047 72-73 22.367342489712932 26.967666802083944 27.98323373324759 22.68175697495553 74-75 22.19895087898651 27.49980581113316 27.66896937077796 22.632273939102372 76-77 22.37207277192367 27.157284083647614 27.640456829259126 22.830186315169588 78-79 22.392972586949984 27.219667735792324 27.74437456149143 22.642985115766262 80-81 22.440886927849103 27.515672449562523 27.505838018781887 22.53760260380649 82-83 22.391010574413144 27.443866981304915 27.580098869901676 22.585023574380262 84-85 22.335807524794895 27.429858848635043 27.411005678812767 22.823327947757303 86-87 22.429964492920192 27.394692289721235 27.42228246198287 22.7530607553757 88-89 22.36035076214447 27.437702013772558 27.208775254363935 22.993171969719032 90-91 22.381552948958177 27.346838045241967 27.446305350219806 22.82530365558005 92-93 22.478362699843967 27.221812628528724 27.581646082234585 22.718178589392725 94-95 22.25580803904766 27.127996101247298 27.557397607629124 23.05879825207591 96-97 22.228399239673532 27.031303488838613 27.6819156869482 23.058381584539653 98-99 22.170869369514698 27.17953183530571 27.264480695277598 23.385118099901987 100-101 22.2382453919205 27.026726500885328 27.374436939935144 23.360591167259027 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 276.0 1 246.0 2 734.5 3 1035.5 4 630.0 5 491.0 6 778.0 7 1532.5 8 2382.0 9 2716.5 10 2356.0 11 1980.0 12 2023.5 13 2131.5 14 2312.5 15 2442.0 16 2516.0 17 2632.5 18 2788.0 19 3041.0 20 3355.5 21 3808.0 22 4415.0 23 5235.0 24 6200.0 25 7540.0 26 9261.5 27 11270.5 28 13294.5 29 15443.5 30 18601.0 31 22419.0 32 26601.0 33 29267.0 34 31350.0 35 33680.0 36 36557.0 37 39622.5 38 40878.0 39 41664.0 40 42753.5 41 45222.0 42 47654.0 43 49606.5 44 51858.0 45 54895.0 46 56391.5 47 58134.0 48 64446.5 49 64510.0 50 58486.5 51 54844.5 52 52638.0 53 52394.5 54 52053.5 55 49316.5 56 45447.5 57 41215.5 58 38089.5 59 34470.0 60 29288.5 61 24753.5 62 20978.0 63 16541.5 64 12477.5 65 9865.5 66 7508.0 67 6080.5 68 4653.5 69 3182.0 70 2398.0 71 1925.5 72 1614.5 73 1390.5 74 1134.5 75 939.5 76 707.5 77 436.0 78 285.5 79 194.5 80 130.5 81 89.0 82 68.0 83 45.5 84 28.5 85 20.0 86 14.5 87 11.5 88 8.5 89 9.5 90 9.0 91 5.5 92 2.5 93 1.5 94 1.0 95 0.5 96 2.0 97 2.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06795686586692605 2 0.02749591162726317 3 0.014342969846567803 4 0.017913054044185115 5 0.023863194373547305 6 0.028184875244347207 7 0.07140168395234626 8 0.05229860184228872 9 0.06507574528639279 10-11 0.0556494703435611 12-13 0.03729798490668614 14-15 0.03754851713108034 16-17 0.04684952596171492 18-19 0.04255916161896429 20-21 0.01678565903441123 22-23 0.012276078995315673 24-25 0.02013652753568362 26-27 0.02289238200401979 28-29 0.005543025464721618 30-31 6.889636170840429E-4 32-33 0.009050476606240383 34-35 0.010491036896507018 36-37 0.004102465174454983 38-39 0.008862577437944734 40-41 0.004039832118356433 42-43 0.001565826402463734 44-45 2.1921569634492276E-4 46-47 4.071148646405708E-4 48-49 7.515966731825922E-4 50-51 0.0013466107061188112 52-53 9.394958414782403E-5 54-55 1.2526611219709871E-4 56-57 0.0029437536366318197 58-59 0.0031629693329767424 60-61 0.004760112263489751 62-63 0.0019416247390550301 64-65 5.636975048869441E-4 66-67 3.757983365912961E-4 68-69 0.002505322243941974 70-71 0.0014092437622173605 72-73 0.018821233357614084 74-75 0.01359137317338521 76-77 0.023863194373547305 78-79 0.01759988876369237 80-81 0.010584986480654842 82-83 0.003664033781765137 84-85 0.0032882354451738414 86-87 0.0011273950097738883 88-89 0.0012839776500202617 90-91 0.006044089913510012 92-93 0.010522353424556291 94-95 0.012088179827020025 96-97 0.011367899681886708 98-99 0.021702353938147352 100-101 0.01888386641371263 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1596601.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.42283174446399 #Duplication Level Percentage of deduplicated Percentage of total 1 81.99620881668015 50.364393378308804 2 10.954429687674846 13.457041831252267 3 3.030726806235183 5.584674680484611 4 1.2605482278037978 3.0970576680869977 5 0.6800473378760893 2.0885216606316845 6 0.4132638710160684 1.5230302329291503 7 0.2888349239836363 1.241874125644036 8 0.20172593975046785 0.9912462764629495 9 0.15207967121109975 0.8407047650897506 >10 0.9076843028010902 10.34486302682146 >50 0.0739682871643628 3.1623597671556314 >100 0.03638288645763331 4.181444177614375 >500 0.0031769120428404803 1.3769602188714372 >1k 7.173672354801085E-4 0.8602932713744426 >5k 2.0496206728003101E-4 0.8855349192723828 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7787 0.4877236078394039 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6281 0.3933982253549885 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3876 0.2427657254379773 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2648 0.1658523325489587 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1781 0.1115494729115164 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1673 0.10478510285287307 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1606 0.10058868809427025 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 3.131652804927468E-5 22-23 0.0 0.0 0.0 0.0 9.394958414782404E-5 24-25 0.0 0.0 0.0 2.5053222439419743E-4 1.2526611219709871E-4 26-27 0.0 0.0 0.0 4.071148646405708E-4 1.2526611219709871E-4 28-29 0.0 0.0 0.0 7.202801451333176E-4 1.2526611219709871E-4 30-31 0.0 0.0 0.0 0.0014092437622173605 1.2526611219709871E-4 32-33 0.0 0.0 0.0 0.0029437536366318197 1.2526611219709871E-4 34-35 0.0 0.0 0.0 0.005323809768376695 1.2526611219709871E-4 36-37 0.0 0.0 0.0 0.007891765068417218 1.2526611219709871E-4 38-39 0.0 0.0 0.0 0.010741569120901216 1.2526611219709871E-4 40-41 0.0 0.0 0.0 0.013967171509976506 1.2526611219709871E-4 42-43 0.0 0.0 0.0 0.021263922545457503 1.2526611219709871E-4 44-45 0.0 0.0 0.0 0.0312852115212254 1.2526611219709871E-4 46-47 0.0 0.0 0.0 0.04907299945321342 1.2526611219709871E-4 48-49 0.0 0.0 0.0 0.07603653010363892 1.2526611219709871E-4 50-51 0.0 0.0 0.0 0.10068263767841809 1.2526611219709871E-4 52-53 0.0 0.0 0.0 0.12501557997270452 1.565826402463734E-4 54-55 0.0 0.0 0.0 0.1562068419097821 1.8789916829564806E-4 56-57 0.0 0.0 0.0 0.1893397285859147 1.8789916829564806E-4 58-59 0.0 0.0 0.0 0.22651244738040374 1.8789916829564806E-4 60-61 0.0 0.0 0.0 0.2775583881007215 1.8789916829564806E-4 62-63 0.0 0.0 0.0 0.33007620563935514 1.8789916829564806E-4 64-65 0.0 0.0 0.0 0.387416768497577 1.8789916829564806E-4 66-67 0.0 0.0 0.0 0.4669607497427347 1.8789916829564806E-4 68-69 0.0 0.0 0.0 0.5595010901283415 2.5053222439419743E-4 70-71 0.0 0.0 0.0 0.6537638395566582 3.131652804927468E-4 72-73 0.0 0.0 0.0 0.7538201466740908 4.384313926898455E-4 74-75 0.0 0.0 0.0 0.8503376861219554 4.384313926898455E-4 76-77 0.0 0.0 0.0 0.9549662063345821 4.384313926898455E-4 78-79 0.0 0.0 0.0 1.0794494053304489 4.384313926898455E-4 80-81 0.0 0.0 0.0 1.2380049868439267 4.697479207391202E-4 82-83 0.0 0.0 0.0 1.4078971515112417 5.010644487883949E-4 84-85 0.0 0.0 0.0 1.587027691953093 5.010644487883949E-4 86-87 0.0 0.0 0.0 1.814385685590827 5.010644487883949E-4 88-89 0.0 0.0 0.0 2.071901495740013 5.010644487883949E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1960 0.0 35.40707 2 GTACCTG 2040 0.0 35.184677 1 TATAACG 110 6.973096E-8 34.569294 2 GTATCAA 11280 0.0 28.69755 1 GTACATG 8450 0.0 27.564249 1 GTACACG 260 0.0 27.423616 1 GGTATCA 7930 0.0 26.794222 1 TAACGCA 180 6.6465873E-9 26.409582 4 TACATGG 8695 0.0 26.40406 2 ACATGGG 8615 0.0 25.547365 3 ATCAACG 13450 0.0 23.856234 3 TCAACGC 13465 0.0 23.795101 4 ATAACGC 200 2.0389052E-8 23.76788 3 TATCAAC 13575 0.0 23.63508 2 CAACGCA 13625 0.0 23.517147 5 ACCTGGG 2855 0.0 23.310005 3 AACGCAG 13880 0.0 23.085096 6 CATGGGG 3615 0.0 22.749498 4 GTATAGG 410 0.0 22.028074 1 GAGTACT 7155 0.0 21.888285 12-13 >>END_MODULE