FastQCFastQC Report
Thu 26 May 2016
SRR936392_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936392_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1596601
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75300.47162691242207666No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67030.41982937502857637No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44390.2780281360214606No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30420.19052975665178715No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19170.12006756854091913No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17030.10666409453582955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG18500.039.799162
GTACCTG20350.036.8882681
GTACACG3550.034.79691
GGTATCA86750.027.3291841
GTATCAA119950.025.9439811
TATCACG950.001226428925.0011712
GTACATG79750.023.7108961
TACATGG82800.022.8331952
ACCTGGG31450.022.5022093
ACATGGG83450.021.7987693
TATCAAC145600.021.336852
TCAACGC146500.021.233544
ATCAACG145950.021.217933
GAGTACT83900.021.17235612-13
CAACGCA148300.021.0078415
TACACGG5050.020.6940362
AACGCAG151600.020.6445276
TAAGGTG8000.020.184025
AGTACTT85450.020.121312-13
CATGGGA47350.019.8592994
Produced by FastQC (version 0.11.5)