##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936392_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1596601 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.62934133199215 31.0 31.0 34.0 30.0 34.0 2 31.7860880708455 31.0 31.0 34.0 30.0 34.0 3 31.856099927283022 31.0 31.0 34.0 30.0 34.0 4 35.61476474084633 37.0 35.0 37.0 33.0 37.0 5 35.436986448085655 37.0 35.0 37.0 33.0 37.0 6 35.52915349545691 37.0 35.0 37.0 33.0 37.0 7 35.46799732682116 37.0 35.0 37.0 33.0 37.0 8 35.433501544844326 37.0 35.0 37.0 33.0 37.0 9 37.010236120358186 39.0 37.0 39.0 33.0 39.0 10-11 36.87852788517607 39.0 37.0 39.0 33.0 39.0 12-13 36.75266300096267 39.0 36.0 39.0 32.0 39.0 14-15 37.78897169674828 39.0 37.0 41.0 32.5 41.0 16-17 37.67185383198432 39.0 37.0 41.0 32.0 41.0 18-19 37.83751607320802 39.5 37.0 41.0 32.5 41.0 20-21 37.859159865238716 40.0 37.0 41.0 33.0 41.0 22-23 37.75417089178825 39.0 37.0 41.0 32.0 41.0 24-25 37.60220086295824 39.0 36.5 41.0 32.0 41.0 26-27 37.358533847843006 39.0 36.0 41.0 32.0 41.0 28-29 37.24947654423366 39.0 36.0 41.0 31.5 41.0 30-31 37.17177522749892 39.0 36.0 41.0 31.0 41.0 32-33 36.9681460803294 39.0 35.5 40.5 31.0 41.0 34-35 36.741727269367864 39.0 35.0 40.0 30.5 41.0 36-37 36.492039964900435 39.0 35.0 40.0 30.0 41.0 38-39 36.291235568560964 38.0 35.0 40.0 30.0 41.0 40-41 36.062141073442895 38.0 35.0 40.0 30.0 41.0 42-43 35.73842149666699 38.0 35.0 40.0 29.0 41.0 44-45 35.40760997894903 38.0 34.5 40.0 27.5 41.0 46-47 34.969508975630106 38.0 34.0 40.0 26.0 41.0 48-49 34.530588731937414 38.0 33.5 40.0 24.0 41.0 50-51 34.19250708223282 38.0 33.0 40.0 23.5 41.0 52-53 33.80073199252662 37.5 33.0 40.0 23.0 41.0 54-55 33.57142611084423 37.0 33.0 40.0 22.0 41.0 56-57 33.24134646038679 37.0 32.0 40.0 21.5 41.0 58-59 32.83241868193744 36.0 31.5 39.0 19.5 40.5 60-61 32.38372548933641 36.0 31.0 39.0 18.0 40.0 62-63 32.01258392046604 35.0 31.0 39.0 17.5 40.0 64-65 31.55884375620459 35.0 30.0 38.0 13.5 40.0 66-67 31.111119810146683 35.0 29.5 38.0 12.0 40.0 68-69 30.715574210463352 34.0 29.0 37.5 8.0 39.5 70-71 30.312234553279122 34.0 29.0 37.0 7.5 39.0 72-73 29.828429582594524 34.0 29.0 36.0 7.0 39.0 74-75 29.295230304878928 34.0 28.0 36.0 3.5 38.5 76-77 27.910536821660514 31.5 26.0 34.5 2.0 36.5 78-79 28.671523442613402 33.0 28.0 35.0 2.0 37.0 80-81 28.434255646839755 33.0 27.0 35.0 2.0 37.0 82-83 28.088183271838112 33.0 26.5 35.0 2.0 36.0 84-85 27.67070889971884 33.0 26.0 35.0 2.0 36.0 86-87 27.220741437591485 32.5 26.0 35.0 2.0 35.5 88-89 26.673440014130016 32.0 25.0 34.5 2.0 35.0 90-91 26.114148118409044 32.0 24.0 34.0 2.0 35.0 92-93 25.395872231070882 31.5 21.5 34.0 2.0 35.0 94-95 24.740502479955857 31.0 19.0 34.0 2.0 35.0 96-97 23.880461680783114 31.0 7.5 34.0 2.0 35.0 98-99 22.82540722447249 31.0 2.0 34.0 2.0 35.0 100-101 19.550925058922047 26.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3040436577964307 1101 2 0.2848555481466484 1101 3 0.3258895126679455 1101 4 0.26449447140225857 1101 5 0.06605170235280156 1101 6 0.19872176369791106 1101 7 0.17615365390659576 1101 8 0.14451670871875422 1101 9 0.1425433599412429 1101 10-11 0.3148283598200976 1101 12-13 0.2940393080234003 1101 14-15 0.5225243210708825 1101 16-17 0.4859216656360772 1101 18-19 0.3771365725058047 1101 20-21 0.2414990415749898 1101 22-23 0.22045784013469216 1101 24-25 0.27790565175696 1101 26-27 0.30551054506045006 1101 28-29 0.28286518356067347 1101 30-31 0.43178628446062106 1101 32-33 0.4360181249363606 1101 34-35 0.4065748106481948 1101 36-37 0.4914608930559723 1101 38-39 0.5608838566316336 1101 40-41 0.706207573678391 1101 42-43 0.667057400770048 1101 44-45 0.802374749081153 1101 46-47 0.6907336344589439 1101 48-49 0.8302232712170436 1101 50-51 0.8278688079028811 1101 52-53 0.8730521305873751 1101 54-55 0.8089851486102546 1101 56-57 0.9465537391161192 1101 58-59 0.9662484565355598 1101 60-61 1.1189273650532883 1101 62-63 1.0472835472002942 1101 64-65 0.9457301851651287 1101 66-67 0.8235750538652482 1101 68-69 0.8276876637761177 1101 70-71 0.7710896046131985 1101 72-73 1.075019157248704 1101 74-75 1.0567871432564004 1101 76-77 0.9226662120761446 1101 78-79 0.9154298055618924 1101 80-81 0.9837049768691379 1101 82-83 1.069100576202004 1101 84-85 1.054116372431885 1101 86-87 0.9790381062599636 1101 88-89 0.9772312867545985 1101 90-91 1.2669646396971856 1101 92-93 0.9991782051331661 1101 94-95 0.901262569323702 1101 96-97 1.024846777934755 1101 98-99 1.2057133453851954 1101 100-101 1.8784660115819172 1102 1 0.2030283659261869 1102 2 0.21496675886459826 1102 3 0.29113719427119733 1102 4 0.2333996907371514 1102 5 0.29081136120255024 1102 6 0.257448242684994 1102 7 0.2475010172120662 1102 8 0.3409074517540418 1102 9 0.3660160603714502 1102 10-11 0.29521485338657527 1102 12-13 0.27933445196796924 1102 14-15 0.480934500762892 1102 16-17 0.4860962948226515 1102 18-19 0.4004870800882969 1102 20-21 0.29302845065266325 1102 22-23 0.3176268488309475 1102 24-25 0.31937857677838366 1102 26-27 0.27925256461765713 1102 28-29 0.28365648693200995 1102 30-31 0.48612704897166026 1102 32-33 0.3986581058524479 1102 34-35 0.2886265148450704 1102 36-37 0.2010944287117269 1102 38-39 0.09283354312386649 1102 40-41 0.1380197139121364 1102 42-43 0.18593242083416328 1102 44-45 0.25908764727852684 1102 46-47 -0.04169765426955507 1102 48-49 0.029987640772368707 1102 50-51 0.09608512223414323 1102 52-53 0.1289919020753345 1102 54-55 0.133009207785598 1102 56-57 -0.09387918265698403 1102 58-59 0.15355959213599135 1102 60-61 0.18674296805215107 1102 62-63 0.03476417303577506 1102 64-65 0.13076876219579603 1102 66-67 0.09573186548206536 1102 68-69 0.09055137941572156 1102 70-71 0.1516530747885625 1102 72-73 0.3829418027048277 1102 74-75 0.10644781384516833 1102 76-77 0.10373193545970594 1102 78-79 0.32329728659376755 1102 80-81 0.27168515954934946 1102 82-83 0.37184969322227346 1102 84-85 0.3465466650920028 1102 86-87 0.4984769835069045 1102 88-89 0.3565215975469691 1102 90-91 0.4898013155695544 1102 92-93 0.5085617085911736 1102 94-95 0.48757934952313065 1102 96-97 0.8577003171814326 1102 98-99 0.7495030810590997 1102 100-101 1.548476327551306 1103 1 0.1712215441135463 1103 2 0.20405299662573384 1103 3 0.14906570223493887 1103 4 0.16538946633426832 1103 5 0.2259393631984139 1103 6 0.08497053197081783 1103 7 -0.010811781236760964 1103 8 0.06522279754982918 1103 9 0.09781806761082379 1103 10-11 0.17862011352672624 1103 12-13 0.10712701388995072 1103 14-15 0.17815364700773983 1103 16-17 0.19043225574582578 1103 18-19 0.24728079313097595 1103 20-21 0.12255535317505917 1103 22-23 0.11005527582231167 1103 24-25 0.1402414358470736 1103 26-27 0.03837836941735162 1103 28-29 0.004664002043206494 1103 30-31 0.07353890945407926 1103 32-33 0.09192930201130167 1103 34-35 0.08878561460740286 1103 36-37 0.0691083734666833 1103 38-39 0.09434356837849123 1103 40-41 0.20540842067398302 1103 42-43 0.14393949387999072 1103 44-45 0.06862531693752061 1103 46-47 0.07040749601078744 1103 48-49 -0.048575252873710895 1103 50-51 0.08380030083292667 1103 52-53 0.17884243145955026 1103 54-55 0.053674888194230164 1103 56-57 -0.22722426063118206 1103 58-59 0.11655631384928 1103 60-61 0.011309765879467193 1103 62-63 0.05181432775865602 1103 64-65 0.07718803274417496 1103 66-67 -0.04660114542075178 1103 68-69 0.011307880183867525 1103 70-71 -0.17269159613535479 1103 72-73 -0.23819395513088892 1103 74-75 0.010173855872015025 1103 76-77 0.02987091030574618 1103 78-79 -0.36979555150964316 1103 80-81 -0.2004253305304431 1103 82-83 -0.10055076547525132 1103 84-85 0.2589487870057532 1103 86-87 0.3985067463235161 1103 88-89 0.34424990680014034 1103 90-91 0.33875939814386413 1103 92-93 0.47086031717949695 1103 94-95 0.2743867622152223 1103 96-97 0.5469316486735565 1103 98-99 0.7712288429653213 1103 100-101 1.2389703903070775 1104 1 0.20981226697330513 1104 2 0.27873412578157186 1104 3 0.22753486463961892 1104 4 0.16560193958732583 1104 5 0.14601937332844983 1104 6 0.09410258946307692 1104 7 0.12035933270255583 1104 8 0.19220668606789815 1104 9 0.1495349158813113 1104 10-11 0.35041439649388195 1104 12-13 0.14033722720022013 1104 14-15 0.20615719252101883 1104 16-17 0.3722889715102653 1104 18-19 0.23518269487389887 1104 20-21 0.3983960712058874 1104 22-23 0.332499748405759 1104 24-25 0.3228096150800681 1104 26-27 0.3016531187954854 1104 28-29 0.30451741696054313 1104 30-31 0.24394460459573963 1104 32-33 0.2446728897187498 1104 34-35 0.2238960685060789 1104 36-37 0.2748736956855993 1104 38-39 0.33388569924913725 1104 40-41 0.3510481046683154 1104 42-43 0.4163559586255303 1104 44-45 0.5281244809947765 1104 46-47 0.4427454100385191 1104 48-49 0.5866757767630943 1104 50-51 0.44044953324647196 1104 52-53 0.5280368573632614 1104 54-55 0.45841604640953904 1104 56-57 0.5052311360821378 1104 58-59 0.39416549612772656 1104 60-61 0.5183256756191028 1104 62-63 0.4198287330159758 1104 64-65 0.6134018452225796 1104 66-67 0.5029233651932543 1104 68-69 0.7118429264665522 1104 70-71 0.694219665161679 1104 72-73 0.8173753122227012 1104 74-75 0.6860609317035049 1104 76-77 0.6769292288482234 1104 78-79 0.6504033577943282 1104 80-81 0.7836112749625279 1104 82-83 0.5957434005243059 1104 84-85 0.8100614815314522 1104 86-87 0.8632072126915773 1104 88-89 0.8327334272458664 1104 90-91 1.05936887445095 1104 92-93 1.0843800546569895 1104 94-95 1.1032867731608142 1104 96-97 1.3224246831993902 1104 98-99 1.3912429649249667 1104 100-101 2.224150917455365 1105 1 0.011848797748957196 1105 2 -0.013545377230183675 1105 3 0.028781189163115783 1105 4 0.18024804095385605 1105 5 0.13278873700430438 1105 6 0.115731363946594 1105 7 0.009545469592914912 1105 8 0.18195928645693016 1105 9 0.04856363739665426 1105 10-11 0.12712964198657062 1105 12-13 0.09282927503683425 1105 14-15 0.23486113402540099 1105 16-17 0.197656472913863 1105 18-19 0.17666426839252125 1105 20-21 0.15054416504526813 1105 22-23 0.20444486745619628 1105 24-25 0.17833290396597334 1105 26-27 0.12401395169692364 1105 28-29 -0.04290217269333141 1105 30-31 0.20304241289102976 1105 32-33 -6.300386074187259E-4 1105 34-35 0.20938161679606537 1105 36-37 0.09947149365239483 1105 38-39 -0.003632109681269924 1105 40-41 0.20958284090806956 1105 42-43 0.14597932036579664 1105 44-45 0.34900063258241687 1105 46-47 0.301722421295338 1105 48-49 0.3568746725708678 1105 50-51 0.5685862713009655 1105 52-53 0.5698072585124052 1105 54-55 0.3858254432352126 1105 56-57 0.08805896453800699 1105 58-59 0.14558072993535376 1105 60-61 0.40022622185727386 1105 62-63 0.3699049942397252 1105 64-65 0.4180585741386693 1105 66-67 0.31505822623811497 1105 68-69 0.2623379692557748 1105 70-71 0.47615048773771207 1105 72-73 0.46577191498327153 1105 74-75 0.6912473732071227 1105 76-77 0.5657581851130971 1105 78-79 0.6038047748625992 1105 80-81 0.4575406657329282 1105 82-83 0.7344003952062153 1105 84-85 0.8073927240914749 1105 86-87 0.823643716089034 1105 88-89 0.6932794434255136 1105 90-91 0.8481561159651747 1105 92-93 0.7237105591156592 1105 94-95 0.7832122354068076 1105 96-97 0.9256871898396817 1105 98-99 0.7474383761790726 1105 100-101 1.1062416928225112 1106 1 0.12599127839041202 1106 2 0.09435494140238632 1106 3 0.04001896283800477 1106 4 0.05982795286095666 1106 5 0.20562567102998486 1106 6 0.035729745375945754 1106 7 -0.0851594204352466 1106 8 0.12876414643488232 1106 9 0.1497274398833568 1106 10-11 0.056651555111990604 1106 12-13 0.2411428875296906 1106 14-15 0.16893057196106298 1106 16-17 0.11120723423805856 1106 18-19 0.18001590161187409 1106 20-21 -0.01141076717238576 1106 22-23 0.10232074441722716 1106 24-25 0.06681134717703685 1106 26-27 0.04667999477474183 1106 28-29 -0.24444542962134364 1106 30-31 -0.007993635437735236 1106 32-33 0.05135650592617935 1106 34-35 0.1053504975795505 1106 36-37 -0.06358334515240216 1106 38-39 0.1764572442016359 1106 40-41 -0.00351657132493699 1106 42-43 0.23355999011527473 1106 44-45 0.19253409778534092 1106 46-47 0.26403717180647845 1106 48-49 0.02142747341954987 1106 50-51 0.06260477444933343 1106 52-53 -0.1890636692610741 1106 54-55 0.01695742637949138 1106 56-57 0.03768644234696694 1106 58-59 0.060451912414144715 1106 60-61 0.0011591584525874055 1106 62-63 0.0021281010296263503 1106 64-65 -0.004888523067787531 1106 66-67 -0.058461612858394574 1106 68-69 0.06352887339059166 1106 70-71 0.15232155183499074 1106 72-73 0.30895610142253815 1106 74-75 0.21247401764039964 1106 76-77 0.1637900847566982 1106 78-79 0.01584455656885808 1106 80-81 0.2982121467062555 1106 82-83 0.49655190847567354 1106 84-85 0.6846404513485176 1106 86-87 0.7979562783930234 1106 88-89 0.6174561524447775 1106 90-91 0.764588936813869 1106 92-93 0.7016086004569644 1106 94-95 0.7615589909568428 1106 96-97 0.8303823935380521 1106 98-99 0.9810470547290571 1106 100-101 1.4370524398806985 1107 1 -0.1233162862918995 1107 2 -6.930277522343431E-4 1107 3 -0.13055057716529106 1107 4 0.09438123104626328 1107 5 -0.021288267080578294 1107 6 -0.1684996476934728 1107 7 -0.05250405362191657 1107 8 -0.13416321526469233 1107 9 -0.14724386130271228 1107 10-11 -0.11976751309744316 1107 12-13 -0.24902619687615868 1107 14-15 -0.10243974966581249 1107 16-17 0.014594845615405916 1107 18-19 -0.24080830774084205 1107 20-21 -0.26070136418150724 1107 22-23 -0.19298263449436348 1107 24-25 -0.14963831837469854 1107 26-27 -0.2108817259077398 1107 28-29 -0.11397713418145372 1107 30-31 -0.2510149857066608 1107 32-33 -0.24854495816806832 1107 34-35 -0.2251190010226196 1107 36-37 -0.16626229394642422 1107 38-39 -0.24152395438246543 1107 40-41 -0.3173711700888262 1107 42-43 -0.2525962395813579 1107 44-45 -0.32070007029581404 1107 46-47 -0.5011399491064026 1107 48-49 -0.5195482233193829 1107 50-51 -0.3755533013301431 1107 52-53 -0.34637590344704705 1107 54-55 -0.4251237296928707 1107 56-57 -0.4944498679183553 1107 58-59 -0.5048561989357481 1107 60-61 -0.41229830420773084 1107 62-63 -0.26408913824973723 1107 64-65 -0.324480354891616 1107 66-67 -0.45232804580307473 1107 68-69 -0.4434395957365709 1107 70-71 -0.2637069760681925 1107 72-73 -0.08139894868146058 1107 74-75 -0.251717867616172 1107 76-77 -0.3032267638342745 1107 78-79 -0.1851017459900497 1107 80-81 0.08203627769916011 1107 82-83 -0.005418963891024475 1107 84-85 0.1834499376969454 1107 86-87 0.20794045835154762 1107 88-89 -0.042956075632833546 1107 90-91 0.06821434241838631 1107 92-93 0.45041052847824403 1107 94-95 0.4947949298460692 1107 96-97 0.7167022224545434 1107 98-99 0.5368265835707113 1107 100-101 0.9021937435779854 1108 1 -0.0379873592526323 1108 2 0.06253751963011212 1108 3 0.011080214937631894 1108 4 0.10357872874114804 1108 5 0.0036789401141064104 1108 6 0.021925411594182265 1108 7 -0.04527474473416504 1108 8 0.02971053026313797 1108 9 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68-69 -1.1897853996075867 2316 70-71 -1.1712300561993736 2316 72-73 -0.6341536357963946 2316 74-75 -0.7580163264131627 2316 76-77 -0.7718280699706526 2316 78-79 -1.0391353188133152 2316 80-81 -0.6312317172203663 2316 82-83 -0.8901237562172355 2316 84-85 -0.8348267300439502 2316 86-87 -0.6994454205891323 2316 88-89 -0.7501514368582143 2316 90-91 -0.8854168683643522 2316 92-93 -1.0636169307168473 2316 94-95 -1.007938667621275 2316 96-97 -1.1132967349111915 2316 98-99 -2.063173890353955 2316 100-101 -2.482244940048865 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 12.0 4 38.0 5 79.0 6 195.0 7 448.0 8 1028.0 9 1846.0 10 3002.0 11 4689.0 12 6881.0 13 8993.0 14 10538.0 15 13728.0 16 17038.0 17 20748.0 18 18584.0 19 13496.0 20 12719.0 21 13273.0 22 14211.0 23 16285.0 24 18917.0 25 22303.0 26 27607.0 27 33753.0 28 40542.0 29 49253.0 30 58828.0 31 71526.0 32 86869.0 33 106953.0 34 135189.0 35 173531.0 36 225088.0 37 248654.0 38 115303.0 39 4451.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.575426693854794 19.31653808687045 14.789088414662796 22.318946804611958 2 16.392461954291907 21.212013016502397 38.73035858021153 23.665166448994164 3 19.792117528636453 25.59163008094688 29.855826478143467 24.760425912273195 4 13.282780105987657 17.285345555965453 36.281011974814 33.15086236323289 5 13.341029065062548 37.80318385324411 33.62851036856834 15.227276713125 6 31.3631896760681 36.22226216819356 17.417438671277292 14.997109484461053 7 28.370832787903804 33.029103702177316 20.541951307809526 18.058112202109356 8 27.158757886284672 34.00736940538056 19.349480552749245 19.48439215558552 9 26.923821292859017 16.468798403608666 20.16101706061815 36.44636324291417 10-11 25.37199337843331 26.57645836373646 26.74891848370382 21.302629774126412 12-13 26.615607591717804 24.02604438732242 27.566576599260166 21.791771421699604 14-15 22.974640791256668 25.31796483714294 26.482752875721182 25.22464149587921 16-17 21.901401790428544 28.608306020101455 27.145166513111292 22.345125676358716 18-19 21.82214768778841 26.57609793448799 30.135461635905553 21.466292741818044 20-21 22.630137398135165 25.56355658051072 30.327896575287127 21.478409446066987 22-23 22.216867092571714 25.596956031567082 30.32090066391081 21.865276211950395 24-25 22.291073348945666 25.826114351675844 30.364161114768184 21.518651184610306 26-27 22.014618747572936 25.97758959776522 30.298137268411228 21.70965438625061 28-29 21.692232048905147 26.319461842962372 30.359742195720802 21.628563912411686 30-31 21.879694609441376 25.867190369906645 30.378055322679092 21.875059697972883 32-33 21.620792099673597 26.138387250538837 30.282193886467258 21.958626763320307 34-35 22.399198283826316 26.072091821555514 29.844855393576875 21.683854501041292 36-37 22.513648607048204 25.77666234600397 29.802645197597506 21.90704384935032 38-39 22.06567962151682 26.087454868473834 30.031470323976166 21.815395186033175 40-41 22.301905487852615 25.99359638832293 29.88837522759315 21.816122896231303 42-43 22.082047083855688 26.282801906253827 29.43058856542092 22.20456244446957 44-45 22.306287854588273 26.286351365740305 29.262418810324633 22.14494196934679 46-47 22.644007668800995 26.691266874149367 28.5422638997018 22.122461557347837 48-49 22.784492599082107 26.79064946127456 28.222072945977132 22.2027849936662 50-51 22.587168099190844 26.99535074242186 28.196622946650972 22.22085821173632 52-53 23.000151572470433 27.056844687071745 27.859114015067053 22.08388972539077 54-55 22.43332099435852 26.960910503599283 28.121713651854257 22.484054850187935 56-57 22.67475621421202 27.25179640699808 27.755185009092443 22.318262369697457 58-59 22.86389448628439 26.8777934988631 27.81468943826025 22.44362257659226 60-61 22.5813888148463 26.996150794632722 28.263860851022375 22.158599539498596 62-63 22.590231258119843 26.9664895596211 28.28282796645949 22.16045121579957 64-65 22.273892436583136 27.138984002174972 28.158878235365602 22.428245325876283 66-67 22.310109288333987 27.254845036802642 27.830615452694595 22.604430222168773 68-69 22.482307616665757 26.996801309790676 28.024334347368864 22.496556726174706 70-71 22.48353453699566 27.048580436108118 27.864940456263454 22.602944570632776 72-73 22.572702885692795 27.048179225742686 27.854329290787117 22.524788597777405 74-75 22.421052664544014 27.621835710122816 27.42983233291776 22.52727929241541 76-77 22.511108160348346 27.31946655776682 27.533111525053023 22.636313756831818 78-79 22.618750090041672 27.312599006691585 27.5095945274707 22.559056375796047 80-81 22.640818017840818 27.633534421764534 27.278484561000077 22.447162999394575 82-83 22.585633337937573 27.528047480652024 27.389356568759275 22.49696261265113 84-85 22.490644194984156 27.519019371927666 27.238509674373816 22.751826758714362 86-87 22.586155434793163 27.476186732722326 27.17886736762586 22.758790464858656 88-89 22.511861481083503 27.486619121537693 27.069740339687133 22.931779057691674 90-91 22.51254590557659 27.501176423409667 27.177730477167362 22.808547193846383 92-93 22.580193479897243 27.403126138455626 27.254897845583837 22.7617825360633 94-95 22.28919795249105 27.23393018409224 27.34525823628674 23.131613627129962 96-97 22.308642346633782 27.169390545439963 27.396411773773316 23.125555334152942 98-99 22.17852250752883 27.310093704609283 27.01056980974593 23.500813978115957 100-101 22.111918984930305 27.357970612610664 27.011300311295138 23.518810091163886 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 549.0 1 447.0 2 737.0 3 1011.0 4 698.0 5 529.5 6 774.0 7 1443.5 8 2063.0 9 2387.5 10 2269.0 11 1950.0 12 1888.5 13 2049.0 14 2241.5 15 2418.5 16 2697.5 17 2869.5 18 3070.0 19 3348.5 20 3663.0 21 4096.0 22 4777.5 23 5557.0 24 6513.0 25 7859.5 26 9613.5 27 11607.5 28 13625.5 29 16103.0 30 19226.5 31 22781.0 32 26710.5 33 29375.0 34 31599.0 35 33961.0 36 36486.0 37 39670.0 38 41459.0 39 41753.5 40 42494.5 41 45356.5 42 47985.5 43 49641.5 44 51678.5 45 54175.0 46 55340.0 47 57438.0 48 64217.0 49 64161.0 50 58214.5 51 54928.0 52 52503.0 53 52329.5 54 51948.5 55 49000.0 56 45241.5 57 41197.5 58 37915.0 59 34163.0 60 28881.0 61 24357.0 62 20748.0 63 16515.0 64 12501.5 65 9795.5 66 7411.0 67 5948.0 68 4539.5 69 3095.5 70 2329.5 71 1826.5 72 1598.0 73 1408.5 74 1115.0 75 899.0 76 690.5 77 452.0 78 286.5 79 200.0 80 155.5 81 120.0 82 76.5 83 50.0 84 29.5 85 17.0 86 8.5 87 6.5 88 3.0 89 1.5 90 2.0 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019792045727141598 2 0.010397087312359193 3 0.007641232844023021 4 0.0 5 0.002818487524434721 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0011900280658724378 14-15 9.394958414782403E-5 16-17 0.0 18-19 0.0014718768183159098 20-21 0.0 22-23 6.263305609854936E-5 24-25 0.0 26-27 0.0013152941780695364 28-29 0.0025679553000405235 30-31 0.0014092437622173605 32-33 0.006889636170840429 34-35 0.0016284594585622833 36-37 5.950140329362189E-4 38-39 0.0010960784817246137 40-41 1.2526611219709871E-4 42-43 0.004165098230553533 44-45 0.0019416247390550301 46-47 1.2526611219709871E-4 48-49 5.323809768376695E-4 50-51 1.2526611219709871E-4 52-53 3.131652804927468E-4 54-55 0.002129523907350678 56-57 0.013779272341680858 58-59 0.023330813396709633 60-61 0.018633334189318434 62-63 0.01271451038800552 64-65 0.016221961529524282 66-67 0.0027871709963854466 68-69 6.263305609854935E-4 70-71 9.394958414782403E-5 72-73 0.0 74-75 9.081793134289657E-4 76-77 0.0016910925146608328 78-79 0.007390700619628824 80-81 0.012871093028251894 82-83 0.015376415272193867 84-85 0.02502190591137047 86-87 0.010616303008704116 88-89 0.03592005767251806 90-91 0.041463083137239676 92-93 0.02596140175284871 94-95 0.02614930092114436 96-97 0.04478263511046279 98-99 0.04603529623243378 100-101 0.05314414809961912 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1596601.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.00349326069625 #Duplication Level Percentage of deduplicated Percentage of total 1 81.25229302899123 48.754214171811924 2 11.263359256474564 13.51681802477344 3 3.2535703188498917 5.856767541009325 4 1.348377889836814 3.236295345027804 5 0.7072913543511011 2.121997600707751 6 0.4155745119978315 1.4961553457987409 7 0.29551711087205806 1.2412441279442374 8 0.21364527083804047 1.025557005512799 9 0.16199404621198327 0.8748187794138289 >10 0.9663784930319435 10.80275077733214 >50 0.07687804142543259 3.191369608686228 >100 0.040294043472343444 4.4156993229384405 >500 0.0038822922811165158 1.640290533813299 >1k 7.344877288598814E-4 0.9299156022117385 >5k 2.0985363681710897E-4 0.8961062130182582 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7530 0.47162691242207666 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6703 0.41982937502857637 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4439 0.2780281360214606 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3042 0.19052975665178715 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1917 0.12006756854091913 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1703 0.10666409453582955 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 6.263305609854936E-5 7 0.0 0.0 0.0 0.0 6.263305609854936E-5 8 0.0 0.0 0.0 0.0 6.263305609854936E-5 9 0.0 0.0 0.0 0.0 6.263305609854936E-5 10-11 0.0 0.0 0.0 0.0 6.263305609854936E-5 12-13 0.0 0.0 0.0 0.0 9.394958414782404E-5 14-15 0.0 0.0 0.0 0.0 1.2526611219709871E-4 16-17 0.0 0.0 0.0 0.0 1.2526611219709871E-4 18-19 0.0 0.0 0.0 0.0 1.2526611219709871E-4 20-21 0.0 0.0 0.0 0.0 1.2526611219709871E-4 22-23 0.0 0.0 0.0 0.0 1.565826402463734E-4 24-25 0.0 0.0 0.0 2.5053222439419743E-4 2.5053222439419743E-4 26-27 0.0 0.0 0.0 4.071148646405708E-4 2.5053222439419743E-4 28-29 0.0 0.0 0.0 7.202801451333176E-4 2.5053222439419743E-4 30-31 0.0 0.0 0.0 0.0014092437622173605 2.5053222439419743E-4 32-33 0.0 0.0 0.0 0.0029437536366318197 2.5053222439419743E-4 34-35 0.0 0.0 0.0 0.00535512629642597 3.131652804927468E-4 36-37 0.0 0.0 0.0 0.007891765068417218 3.131652804927468E-4 38-39 0.0 0.0 0.0 0.010835518705049039 3.131652804927468E-4 40-41 0.0 0.0 0.0 0.01406112109412433 3.131652804927468E-4 42-43 0.0 0.0 0.0 0.02129523907350678 3.131652804927468E-4 44-45 0.0 0.0 0.0 0.031504427217570324 3.131652804927468E-4 46-47 0.0 0.0 0.0 0.04944879778980472 3.131652804927468E-4 48-49 0.0 0.0 0.0 0.07613047968778675 3.131652804927468E-4 50-51 0.0 0.0 0.0 0.10068263767841809 3.131652804927468E-4 52-53 0.0 0.0 0.0 0.12401345107512772 3.131652804927468E-4 54-55 0.0 0.0 0.0 0.15454706592317052 3.131652804927468E-4 56-57 0.0 0.0 0.0 0.18714757162246548 3.131652804927468E-4 58-59 0.0 0.0 0.0 0.22338079457547627 3.444818085420214E-4 60-61 0.0 0.0 0.0 0.2737690882067592 3.757983365912961E-4 62-63 0.0 0.0 0.0 0.32497161156732335 3.757983365912961E-4 64-65 0.0 0.0 0.0 0.3817797934487076 3.757983365912961E-4 66-67 0.0 0.0 0.0 0.45994584745969724 3.757983365912961E-4 68-69 0.0 0.0 0.0 0.5518285407562691 4.071148646405708E-4 70-71 0.0 0.0 0.0 0.6452144273992062 5.010644487883949E-4 72-73 0.0 0.0 0.0 0.7442372890910127 6.263305609854935E-4 74-75 0.0 0.0 0.0 0.8397840161693497 6.263305609854935E-4 76-77 0.0 0.0 0.0 0.9431911917880548 6.263305609854935E-4 78-79 0.0 0.0 0.0 1.0667348949424433 6.263305609854935E-4 80-81 0.0 0.0 0.0 1.222565938515634 6.263305609854935E-4 82-83 0.0 0.0 0.0 1.3892011842658247 6.263305609854935E-4 84-85 0.0 0.0 0.0 1.5654506041271428 6.263305609854935E-4 86-87 0.0 0.0 0.0 1.7877040036928449 6.263305609854935E-4 88-89 0.0 0.0 0.0 2.0389251917041262 6.263305609854935E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1850 0.0 39.79916 2 GTACCTG 2035 0.0 36.888268 1 GTACACG 355 0.0 34.7969 1 GGTATCA 8675 0.0 27.329184 1 GTATCAA 11995 0.0 25.943981 1 TATCACG 95 0.0012264289 25.001171 2 GTACATG 7975 0.0 23.710896 1 TACATGG 8280 0.0 22.833195 2 ACCTGGG 3145 0.0 22.502209 3 ACATGGG 8345 0.0 21.798769 3 TATCAAC 14560 0.0 21.33685 2 TCAACGC 14650 0.0 21.23354 4 ATCAACG 14595 0.0 21.21793 3 GAGTACT 8390 0.0 21.172356 12-13 CAACGCA 14830 0.0 21.007841 5 TACACGG 505 0.0 20.694036 2 AACGCAG 15160 0.0 20.644527 6 TAAGGTG 800 0.0 20.18402 5 AGTACTT 8545 0.0 20.1213 12-13 CATGGGA 4735 0.0 19.859299 4 >>END_MODULE